MD02G1277600.v1.1

zinc finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0000854
Physical Location & Seq
Forward (+)
33344674 .. 33349186
4513 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1277600.v1.1.491

Sequence Viewer

Length: 2667 bp
ATGGACGACAGCTGTGCGGTGTGTGCCGATACTCTGGAATGGGTTGCCTACGGTGCCTGTGGACATCGCGAAGTCTGCTCCACTTGCGTCGTTCGTCTCCGCTTCATCTGCAACGATCGCCGGTGCTGCATCTGCAAGACCGAGTCCGACGTCATTTTTATCACCAAGGGTTTAGGAGATTATACAAGGATGATTAATGACTTCTCAGTCTTGCCGACTGAAGTAAGAGAGGGTCGCGTGGGACCGTATTGGTACCACGAGGACACTCAAGCATTTTTCGATGATGTGGACCACTACAAGATGATCAAGGCAATGTGCAAGCTTTCATGCAGCGAATGTGACAAGATGGATGAGCAATCAAATGATGGCCCAAAGCGTCGCGGGAAGATTCGGAACCTTAATCATCTGAAGAATCATTTATTCCACCAACATAGGTTGTGGATGTGCTCTTTATGTTTGGAAGGGAGGAAGGTGTTTATATGTGAACAAAAACTATATACTAGAGCTCAATTGAGGCGGCATATAAATACAGGTGACTCTGAAGTGGATGGAAGTGAGAGTGAAAGAGGTGGCTTCATGGGACATCCTATGTGTGAGTTTTGCAGAACCCCATTTTATGGGGATAATGAGCTGTACTCCCACATGACTACTGATCACTATACTTGTCATATATGCCAAAGGCAGAATCCTGGACAATATGAATATTATAAGAATTATGATGACCTGGAGATGCACTTCCGCCAAGGTCATTTTCTATGTGAAGATGAGTCCTGCCTCAGCAAAAAGTTTGTTGTCTTCCAATCTGAGGCAGAAATGAAGAGGCATAATACACTTGAACATGGAGGGAGCATGTCTCGTTCCAAGCGTAATGCTGCTCTGCAGATACCAACCAGCTTCAGATATCAACGAAGTAGTGAACAAGATCATCGACGTGGAAGAGGCGGAAGAGGTCTGGCATTTCGTCGTGATTCTTCTGAAAATCAACTTTCTATGGCTGTTCAAGCAAGTTTGGAAACTGCTCATGCTGATAGAACATATGATGATCCTTCATCCTCCAGACTGCAAGTTCCTCCAGATATTGGGGATACAGGTGATATTGATCCGATCGCTGATTCATTTGAATCATTAAGTGCAACAGATATCGAAACATCTTCAAGGTACCGTCAAGCCGTGGGGCCTATTTCTAGGAATGGGCGTTTGGAAGAATCTTCCTTTCCTCCCCTCTCAATGGCAGCTGGCAACAGTTCAGACCCCAAACAAGACTCAGATAGTCTGCCTAGCAACACAATGGCAGCGCATCTCCGCCGGCAAAACAACCACAAGGTGGCTATTAATAGCTCTGGAATGGCTTGGCCAGCAGCAACACGTGGGCCTGTGGCCGTTAATGGTTCTGGACAGGCTTGGCCAGCAGCAACACGTGGGCCTGTGGCTGTTAATAGTTCTGGACAGGCTTGGCCAGCAGGAACTCGGGGGCCTGTAGTACAACCTACTAATTCAGCTCAGGCTTCGCCTGCGACAAGTGTTTCATCTCGTGTTTCTGGTCAGAGCAATATGGCCTTTGGTAATGGACCCAGACCATCTAGTTATGCCAGTTCCACTCAGGCTCAAGTTGAGACTAGACAGAATGCCTTTGGTAATGGATTGAGACCATCTAGTTATGCAAGTTCTGCAGCTCATGTTGAACAGACAGCAATACCTGGATTGTTATCATCAGGTTCTTCAAGGGATTCAAGCAACACCAAATCTGGTAGGATCAGCCACTCTACGTCAGCTCCTAATTTGGTCGAAAATGGATCTGTTCAACCCTCCAATTCTGATTTTCCGCCTGTTTCTGCAGTACAAGTGCGTAAGTTGCCTTCAACTACCCATCCTATATTGAAAGCGGAGGATGTTCAAACTGCTAACAAGTCCTTAGTGGAAAAGATTCGTGCCGGTCTTGAATTTGACGAAGAAAAATATACTATTTTTAAAGACATATCTGGACAGTATCGCCAGGGTTTGGTTGACACAGGAATATATTTAGATTTTGTGCGGCAGTTTGGCTTGCTTCATCTTGTCCTTGATTTGGCTAGACTCTGCCCTGATTCTCAGAAGCAGAAGGAGCTTATCGATGCCTACAATACTGGTACTCGCAATGGATGGTCTCAGGACAGTCCTAAATTGAAAGATGGTAAAAATTCTAAGAAAGGTAAAGGGAAGATTTCAGAGTCTGAAAACAGTAATTCTAAGAACACGCTAGCAGATAGCATTATTAGCTCTGTACGGGAACTTCAATCAAACTACAGGCCTACGGAAGAAGCAGTGGAGGTGTTGACAAAGGATGGGTACCGTGCTGCCAAAGGAAAATCAAATCTGTTAGTGAATGGGCACCAGGAGGAGTTGAATTCTCGCAGTCAACCTCTAGTGCAACCGAGGGGCCAAAAGGATTCCACACCAGCTGCTAGGCCGAATTTAGTGGATGGTGGCGGAGGGAGTAAGCAGCGGAAGAAAACCTCCAAATTTGATAGAGTCCGCTTGGGTGATGCTGCAGCACTTTTAGATAGCAGAAATTCTGATTCTCAGCCAGATGCCGGTGATGAAGGATTAGATGGGAGGAGCAGTAACTCGGCTGGAGGATTGCCTGTCAAAGGTGTTTGGCGAAAAGGAACTCAAAAACTCTTTAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

889

Amino Acids

97.81

Weight (kDa)

8.4

Isoelectric Point (pI)

50.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RING_ZNF598 PF25447 2 - 60 1.1e-07 ZNF598 RING finger domain
zf-C2H2_13 PF23230 219 - 275 1.6e-17 C2H2-type zinc finger
PAH_ZNF598 PF23202 632 - 707 1.6e-20 ZNF598 PAH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 708
AasI GACNNNNNNGTC 1 cut(s) 206
AatII GACGTC 1 cut(s) 153
Acc65I GGTACC 3 cut(s) 252, 1158, 2325
AccB1I GGYRCC 5 cut(s) 53, 252, 1158, 2325, 2367
AccB7I CCANNNNNTGG 4 cut(s) 617, 1079, 1324, 1999
AccII CGCG 3 cut(s) 69, 237, 381
AclWI GGATC 4 cut(s) 1037, 1094, 1760, 1801
AcoI YGGCCR 4 cut(s) 1352, 1377, 1403, 1454
AcsI RAATTY 6 cut(s) 1940, 2176, 2383, 2449, 2498, 2548
AcuI CTGAAG 4 cut(s) 240, 428, 561, 880
AcvI CACGTG 2 cut(s) 1367, 1418
AcyI GRCGYC 1 cut(s) 150
AdeI CACNNNGTG 1 cut(s) 1324
AfaI GTAC 8 cut(s) 254, 635, 1160, 1482, 1839, 2128, 2262, 2327
AfiI CCNNNNNNNGG 9 cut(s) 617, 805, 1079, 1228, 1324, 1999, 2065, 2570, 2627
AflIII ACRYGT 2 cut(s) 1364, 1415
AjiI CACGTC 1 cut(s) 932
AjnI CCWGG 5 cut(s) 688, 723, 1696, 1992, 2370
AjuI GAANNNNNNNTTGG 4 cut(s) 735, 767, 1181, 1213
Alw21I GWGCWC 2 cut(s) 449, 508
Alw26I GTCTC 5 cut(s) 101, 858, 1607, 1639, 2148
AlwI GGATC 4 cut(s) 1037, 1094, 1760, 1801
AlwNI CAGNNNCTG 1 cut(s) 2210
Ama87I CYCGRG 1 cut(s) 1467
ApoI RAATTY 6 cut(s) 1940, 2176, 2383, 2449, 2498, 2548
AseI ATTAAT 2 cut(s) 195, 1332
Asp700I GAANNNNTTC 1 cut(s) 1923
Asp718I GGTACC 3 cut(s) 252, 1158, 2325
AspLEI GCGC 1 cut(s) 1297
AspS9I GGNCC 9 cut(s) 242, 289, 368, 1175, 1370, 1421, 1472, 1568, 2416
AsuHPI GGTGA 5 cut(s) 154, 545, 1103, 2531, 2585
AsuNHI GCTAGC 1 cut(s) 2236
AvaI CYCGRG 1 cut(s) 1467
AvaII GGWCC 3 cut(s) 242, 289, 1568
BaeGI GKGCMC 1 cut(s) 2370
BalI TGGCCA 3 cut(s) 1354, 1405, 1456
BanI GGYRCC 5 cut(s) 53, 252, 1158, 2325, 2367
BanII GRGCYC 1 cut(s) 508
BarI GAAGNNNNNNTAC 4 cut(s) 1840, 1872, 2253, 2285
BauI CACGAG 2 cut(s) 257, 1530
BbrPI CACGTG 2 cut(s) 1367, 1418
BbsI GAAGAC 1 cut(s) 787
Bbv12I GWGCWC 2 cut(s) 449, 508
BbvCI CCTCAGC 1 cut(s) 776
BceAI ACGGC 2 cut(s) 1154, 1364
BcgI CGANNNNNNTGC 1 cut(s) 30
BciT130I CCWGG 5 cut(s) 690, 725, 1698, 1994, 2372
BciVI GTATCC 1 cut(s) 1078
BclI TGATCA 2 cut(s) 303, 652
BcoDI GTCTC 5 cut(s) 101, 858, 1607, 1639, 2148
BfmI CTRYAG 6 cut(s) 878, 1476, 1668, 1833, 2281, 2526
BfuI GTATCC 1 cut(s) 1078
Bme1390I CCNGG 5 cut(s) 690, 725, 1698, 1994, 2372
Bme18I GGWCC 3 cut(s) 242, 289, 1568
BmeT110I CYCGRG 1 cut(s) 1467
BmgBI CACGTC 1 cut(s) 932
BmgT120I GGNCC 9 cut(s) 242, 289, 368, 1175, 1370, 1421, 1472, 1568, 2416
BmrFI CCNGG 5 cut(s) 690, 725, 1698, 1994, 2372
BmsI GCATC 6 cut(s) 138, 720, 1306, 2101, 2512, 2557
BmtI GCTAGC 1 cut(s) 2240
BpiI GAAGAC 1 cut(s) 787
BplI GAGNNNNNCTC 4 cut(s) 620, 652, 838, 870
BpmI CTGGAG 4 cut(s) 746, 1039, 1056, 2631
Bpu10I CCTNAGC 2 cut(s) 776, 1500
BpuEI CTTGAG 2 cut(s) 252, 1590
Bsa29I ATCGAT 1 cut(s) 2109
BsaAI YACGTR 2 cut(s) 1367, 1418
BsaBI GATNNNNATC 2 cut(s) 1098, 1705
BsaHI GRCGYC 1 cut(s) 150
BsaI GGTCTC 2 cut(s) 1639, 2148
BsaJI CCNNGG 5 cut(s) 165, 742, 1170, 1993, 2411
BsaXI ACNNNNNCTCC 4 cut(s) 1756, 1786, 2584, 2614
Bsc4I CCNNNNNNNGG 9 cut(s) 617, 805, 1079, 1228, 1324, 1999, 2065, 2570, 2627
Bse118I RCCGGY 4 cut(s) 120, 1305, 1931, 2570
Bse1I ACTGG 2 cut(s) 1590, 2128
Bse3DI GCAATG 2 cut(s) 318, 2140
Bse8I GATNNNNATC 2 cut(s) 1098, 1705
BseBI CCWGG 5 cut(s) 690, 725, 1698, 1994, 2372
BseCI ATCGAT 1 cut(s) 2109
BseDI CCNNGG 5 cut(s) 165, 742, 1170, 1993, 2411
BseJI GATNNNNATC 2 cut(s) 1098, 1705
BseLI CCNNNNNNNGG 9 cut(s) 617, 805, 1079, 1228, 1324, 1999, 2065, 2570, 2627
BseMI GCAATG 2 cut(s) 318, 2140
BseMII CTCAG 9 cut(s) 219, 790, 795, 1278, 1514, 1613, 2102, 2159, 2573
BseNI ACTGG 2 cut(s) 1590, 2128
BseRI GAGGAG 2 cut(s) 2390, 2608
BseSI GKGCMC 1 cut(s) 2370
Bsh1236I CGCG 3 cut(s) 69, 237, 381
Bsh1285I CGRYCG 2 cut(s) 118, 1107
BshNI GGYRCC 5 cut(s) 53, 252, 1158, 2325, 2367
BshVI ATCGAT 1 cut(s) 2109
BsiEI CGRYCG 2 cut(s) 118, 1107
BsiHKAI GWGCWC 2 cut(s) 449, 508
BsiHKCI CYCGRG 1 cut(s) 1467
BsiSI CCGG 4 cut(s) 121, 1306, 1932, 2571
BslFI GGGAC 2 cut(s) 255, 594
BslI CCNNNNNNNGG 9 cut(s) 617, 805, 1079, 1228, 1324, 1999, 2065, 2570, 2627
BsmAI GTCTC 5 cut(s) 101, 858, 1607, 1639, 2148
BsmBI CGTCTC 1 cut(s) 101
BsmFI GGGAC 2 cut(s) 255, 594
BsmI GAATGC 1 cut(s) 1630
Bso31I GGTCTC 2 cut(s) 1639, 2148
BsoBI CYCGRG 1 cut(s) 1467
Bsp1286I GDGCHC 3 cut(s) 449, 508, 2370
Bsp143I GATC 9 cut(s) 115, 303, 652, 922, 1042, 1099, 1104, 1752, 1793
Bsp68I TCGCGA 1 cut(s) 69
BspCNI CTCAG 9 cut(s) 218, 789, 796, 1277, 1513, 1612, 2101, 2158, 2572
BspDI ATCGAT 1 cut(s) 2109
BspFNI CGCG 3 cut(s) 69, 237, 381
BspMAI CTGCAG 4 cut(s) 882, 1672, 1837, 2530
BspOI GCTAGC 1 cut(s) 2240
BspPI GGATC 4 cut(s) 1037, 1094, 1760, 1801
BspT107I GGYRCC 5 cut(s) 53, 252, 1158, 2325, 2367
BspTNI GGTCTC 2 cut(s) 1639, 2148
BsrDI GCAATG 2 cut(s) 318, 2140
BsrFI RCCGGY 4 cut(s) 120, 1305, 1931, 2570
BsrI ACTGG 2 cut(s) 1590, 2128
BssAI RCCGGY 4 cut(s) 120, 1305, 1931, 2570
BssECI CCNNGG 5 cut(s) 165, 742, 1170, 1993, 2411
BssMI GATC 9 cut(s) 115, 303, 652, 922, 1042, 1099, 1104, 1752, 1793
BssNI GRCGYC 1 cut(s) 150
BssSI CACGAG 2 cut(s) 257, 1530
BssT1I CCWWGG 2 cut(s) 165, 742
Bst2BI CACGAG 2 cut(s) 257, 1530
Bst2UI CCWGG 5 cut(s) 690, 725, 1698, 1994, 2372
Bst4CI ACNGT 8 cut(s) 53, 246, 1163, 1244, 1986, 2153, 2219, 2330
Bst6I CTCTTC 3 cut(s) 812, 931, 940
BstACI GRCGYC 1 cut(s) 150
BstAPI GCANNNNNTGC 1 cut(s) 1667
BstBAI YACGTR 2 cut(s) 1367, 1418
BstC8I GCNNGC 9 cut(s) 320, 1237, 1307, 1356, 1407, 1458, 1512, 2045, 2238
BstDSI CCRYGG 1 cut(s) 1170
BstENI CCTNNNNNAGG 1 cut(s) 2625
BstFNI CGCG 3 cut(s) 69, 237, 381
BstHHI GCGC 1 cut(s) 1297
BstKTI GATC 9 cut(s) 118, 306, 655, 925, 1045, 1102, 1107, 1755, 1796
BstMAI GTCTC 5 cut(s) 101, 858, 1607, 1639, 2148
BstMBI GATC 9 cut(s) 115, 303, 652, 922, 1042, 1099, 1104, 1752, 1793
BstMCI CGRYCG 2 cut(s) 118, 1107
BstNI CCWGG 5 cut(s) 690, 725, 1698, 1994, 2372
BstNSI RCATGY 1 cut(s) 853
BstSCI CCNGG 5 cut(s) 688, 723, 1696, 1992, 2370
BstSFI CTRYAG 6 cut(s) 878, 1476, 1668, 1833, 2281, 2526
BstSLI GKGCMC 1 cut(s) 2370
BstUI CGCG 3 cut(s) 69, 237, 381
BstV2I GAAGAC 1 cut(s) 787
BstX2I RGATCY 1 cut(s) 1793
BstYI RGATCY 1 cut(s) 1793
Bsu15I ATCGAT 1 cut(s) 2109
BsuI GTATCC 1 cut(s) 1078
BsuTUI ATCGAT 1 cut(s) 2109
BtgI CCRYGG 1 cut(s) 1170
BtgZI GCGATG 1 cut(s) 50
BtrI CACGTC 1 cut(s) 932
BtsI GCAGTG 1 cut(s) 2307
BtsIMutI CAGTG 1 cut(s) 2307
BtuMI TCGCGA 1 cut(s) 69
Cac8I GCNNGC 9 cut(s) 320, 1237, 1307, 1356, 1407, 1458, 1512, 2045, 2238
CaiI CAGNNNCTG 1 cut(s) 2210
CfoI GCGC 1 cut(s) 1297
Cfr10I RCCGGY 4 cut(s) 120, 1305, 1931, 2570
Cfr13I GGNCC 9 cut(s) 242, 289, 368, 1175, 1370, 1421, 1472, 1568, 2416
ClaI ATCGAT 1 cut(s) 2109
CseI GACGC 2 cut(s) 76, 365
Csp6I GTAC 8 cut(s) 253, 634, 1159, 1481, 1838, 2127, 2261, 2326
CviAII CATG 7 cut(s) 327, 577, 643, 839, 850, 1022, 1676
CviQI GTAC 8 cut(s) 253, 634, 1159, 1481, 1838, 2127, 2261, 2326
DpnI GATC 9 cut(s) 117, 305, 654, 924, 1044, 1101, 1106, 1754, 1795
DpnII GATC 9 cut(s) 115, 303, 652, 922, 1042, 1099, 1104, 1752, 1793
DraI TTTAAA 1 cut(s) 1969
DraIII CACNNNGTG 1 cut(s) 1324
DrdI GACNNNNNNGTC 1 cut(s) 206
DseDI GACNNNNNNGTC 1 cut(s) 206
EaeI YGGCCR 4 cut(s) 1352, 1377, 1403, 1454
Eam1104I CTCTTC 3 cut(s) 812, 931, 940
EarI CTCTTC 3 cut(s) 812, 931, 940
EciI GGCGGA 5 cut(s) 728, 957, 1292, 1812, 2481
Ecl136II GAGCTC 1 cut(s) 506
Eco130I CCWWGG 2 cut(s) 165, 742
Eco147I AGGCCT 1 cut(s) 2287
Eco24I GRGCYC 1 cut(s) 508
Eco31I GGTCTC 2 cut(s) 1639, 2148
Eco32I GATATC 2 cut(s) 902, 1141
Eco47I GGWCC 3 cut(s) 242, 289, 1568
Eco53kI GAGCTC 1 cut(s) 506
Eco57I CTGAAG 4 cut(s) 240, 428, 561, 880
Eco72I CACGTG 2 cut(s) 1367, 1418
Eco88I CYCGRG 1 cut(s) 1467
EcoICRI GAGCTC 1 cut(s) 506
EcoNI CCTNNNNNAGG 1 cut(s) 2625
EcoO109I RGGNCCY 2 cut(s) 1175, 1472
EcoRI GAATTC 1 cut(s) 2383
EcoRII CCWGG 5 cut(s) 688, 723, 1696, 1992, 2370
EcoRV GATATC 2 cut(s) 902, 1141
EcoT14I CCWWGG 2 cut(s) 165, 742
EcoT38I GRGCYC 1 cut(s) 508
ErhI CCWWGG 2 cut(s) 165, 742
Esp3I CGTCTC 1 cut(s) 101
FaeI CATG 7 cut(s) 330, 580, 646, 842, 853, 1025, 1679
FaqI GGGAC 2 cut(s) 255, 594
FatI CATG 7 cut(s) 326, 576, 642, 838, 849, 1021, 1675
FauI CCCGC 1 cut(s) 374
FauNDI CATATG 1 cut(s) 1036
FbaI TGATCA 2 cut(s) 303, 652
FriOI GRGCYC 1 cut(s) 508
GlaI GCGC 1 cut(s) 1296
GsuI CTGGAG 4 cut(s) 746, 1039, 1056, 2631
HapII CCGG 4 cut(s) 121, 1306, 1932, 2571
HgaI GACGC 2 cut(s) 76, 365
HhaI GCGC 1 cut(s) 1297
Hin1I GRCGYC 1 cut(s) 150
Hin1II CATG 7 cut(s) 330, 580, 646, 842, 853, 1025, 1679
Hin6I GCGC 1 cut(s) 1295
HinP1I GCGC 1 cut(s) 1295
HincII GTYRAC 3 cut(s) 2005, 2313, 2396
HindII GTYRAC 3 cut(s) 2005, 2313, 2396
HindIII AAGCTT 1 cut(s) 320
HpaII CCGG 4 cut(s) 121, 1306, 1932, 2571
HphI GGTGA 5 cut(s) 154, 545, 1103, 2531, 2585
Hpy166II GTNNAC 7 cut(s) 62, 289, 485, 917, 2005, 2313, 2396
Hpy8I GTNNAC 7 cut(s) 62, 289, 485, 917, 2005, 2313, 2396
Hpy99I CGWCG 5 cut(s) 92, 152, 381, 933, 966
HpyAV CCTTC 6 cut(s) 455, 463, 1056, 1866, 2092, 2573
HpyCH4III ACNGT 8 cut(s) 53, 246, 1163, 1244, 1986, 2153, 2219, 2330
HpyCH4IV ACGT 5 cut(s) 150, 931, 1366, 1417, 1766
HpySE526I ACGT 5 cut(s) 150, 931, 1366, 1417, 1766
Hsp92I GRCGYC 1 cut(s) 150
Hsp92II CATG 7 cut(s) 330, 580, 646, 842, 853, 1025, 1679
HspAI GCGC 1 cut(s) 1295
KpnI GGTACC 3 cut(s) 256, 1162, 2329
KroI GCCGGC 1 cut(s) 1305
KroNI GCCGGC 1 cut(s) 1307
Ksp22I TGATCA 2 cut(s) 303, 652
Kzo9I GATC 9 cut(s) 115, 303, 652, 922, 1042, 1099, 1104, 1752, 1793
LmnI GCTCC 5 cut(s) 83, 846, 1777, 2101, 2595
LweI GCATC 6 cut(s) 138, 720, 1306, 2101, 2512, 2557
MaeII ACGT 5 cut(s) 150, 931, 1366, 1417, 1766
MaeIII GTNAC 3 cut(s) 338, 533, 2600
MalI GATC 9 cut(s) 117, 305, 654, 924, 1044, 1101, 1106, 1754, 1795
MboI GATC 9 cut(s) 115, 303, 652, 922, 1042, 1099, 1104, 1752, 1793
MfeI CAATTG 1 cut(s) 509
MflI RGATCY 1 cut(s) 1793
MhlI GDGCHC 3 cut(s) 449, 508, 2370
MlsI TGGCCA 3 cut(s) 1354, 1405, 1456
MluNI TGGCCA 3 cut(s) 1354, 1405, 1456
MlyI GAGTC 7 cut(s) 152, 530, 776, 1256, 2067, 2216, 2517
MmeI TCCRAC 1 cut(s) 171
Mox20I TGGCCA 3 cut(s) 1354, 1405, 1456
MroNI GCCGGC 1 cut(s) 1305
MroXI GAANNNNTTC 1 cut(s) 1923
MscI TGGCCA 3 cut(s) 1354, 1405, 1456
MseI TTAA 7 cut(s) 195, 399, 1127, 1332, 1383, 1434, 1968
MslI CAYNNNNRTG 1 cut(s) 930
Msp20I TGGCCA 3 cut(s) 1354, 1405, 1456
MspA1I CMGCKG 4 cut(s) 12, 1235, 2438, 2482
MspI CCGG 4 cut(s) 121, 1306, 1932, 2571
MspR9I CCNGG 5 cut(s) 690, 725, 1698, 1994, 2372
MunI CAATTG 1 cut(s) 509
Mva1269I GAATGC 1 cut(s) 1630
MvaI CCWGG 5 cut(s) 690, 725, 1698, 1994, 2372
MvnI CGCG 3 cut(s) 69, 237, 381
NaeI GCCGGC 1 cut(s) 1307
NdeI CATATG 1 cut(s) 1036
NdeII GATC 9 cut(s) 115, 303, 652, 922, 1042, 1099, 1104, 1752, 1793
NgoMIV GCCGGC 1 cut(s) 1305
NheI GCTAGC 1 cut(s) 2236
NlaIII CATG 7 cut(s) 330, 580, 646, 842, 853, 1025, 1679
NmeAIII GCCGAG 1 cut(s) 2585
NmuCI GTSAC 2 cut(s) 338, 533
NruI TCGCGA 1 cut(s) 69
NspI RCATGY 1 cut(s) 853
PceI AGGCCT 1 cut(s) 2287
PcsI WCGNNNNNNNCGW 1 cut(s) 862
PctI GAATGC 1 cut(s) 1630
PdiI GCCGGC 1 cut(s) 1307
PdmI GAANNNNTTC 1 cut(s) 1923
PflFI GACNNNGTC 1 cut(s) 142
PflMI CCANNNNNTGG 4 cut(s) 617, 1079, 1324, 1999
PfoI TCCNGGA 1 cut(s) 688
Ple19I CGATCG 2 cut(s) 118, 1107
PleI GAGTC 7 cut(s) 151, 530, 775, 1256, 2067, 2215, 2516
PmaCI CACGTG 2 cut(s) 1367, 1418
PmlI CACGTG 2 cut(s) 1367, 1418
PpsI GAGTC 7 cut(s) 151, 530, 775, 1256, 2067, 2215, 2516
Ppu21I YACGTR 2 cut(s) 1367, 1418
PshBI ATTAAT 2 cut(s) 195, 1332
PsiI TTATAA 1 cut(s) 708
Psp124BI GAGCTC 1 cut(s) 508
Psp6I CCWGG 5 cut(s) 688, 723, 1696, 1992, 2370
PspCI CACGTG 2 cut(s) 1367, 1418
PspGI CCWGG 5 cut(s) 688, 723, 1696, 1992, 2370
PspPI GGNCC 9 cut(s) 242, 289, 368, 1175, 1370, 1421, 1472, 1568, 2416
PstI CTGCAG 4 cut(s) 882, 1672, 1837, 2530
PstNI CAGNNNCTG 1 cut(s) 2210
PsuI RGATCY 1 cut(s) 1793
PsyI GACNNNGTC 1 cut(s) 142
PvuI CGATCG 2 cut(s) 118, 1107
PvuII CAGCTG 3 cut(s) 12, 1235, 2438
RruI TCGCGA 1 cut(s) 69
RsaI GTAC 8 cut(s) 254, 635, 1160, 1482, 1839, 2128, 2262, 2327
RsaNI GTAC 8 cut(s) 253, 634, 1159, 1481, 1838, 2127, 2261, 2326
RseI CAYNNNNRTG 1 cut(s) 930
SacI GAGCTC 1 cut(s) 508
SaqAI TTAA 7 cut(s) 195, 399, 1127, 1332, 1383, 1434, 1968
Sau3AI GATC 9 cut(s) 115, 303, 652, 922, 1042, 1099, 1104, 1752, 1793
Sau96I GGNCC 9 cut(s) 242, 289, 368, 1175, 1370, 1421, 1472, 1568, 2416
SchI GAGTC 7 cut(s) 152, 530, 776, 1256, 2067, 2216, 2517
ScrFI CCNGG 5 cut(s) 690, 725, 1698, 1994, 2372
SduI GDGCHC 3 cut(s) 449, 508, 2370
SfaNI GCATC 6 cut(s) 138, 720, 1306, 2101, 2512, 2557
SfcI CTRYAG 6 cut(s) 878, 1476, 1668, 1833, 2281, 2526
SgrAI CRCCGGYG 1 cut(s) 120
SinI GGWCC 3 cut(s) 242, 289, 1568
SmiMI CAYNNNNRTG 1 cut(s) 930
SmlI CTYRAG 2 cut(s) 267, 1605
SmoI CTYRAG 2 cut(s) 267, 1605
SseBI AGGCCT 1 cut(s) 2287
SspI AATATT 1 cut(s) 704
SstI GAGCTC 1 cut(s) 508
StuI AGGCCT 1 cut(s) 2287
StyD4I CCNGG 5 cut(s) 688, 723, 1696, 1992, 2370
StyI CCWWGG 2 cut(s) 165, 742
TaaI ACNGT 8 cut(s) 53, 246, 1163, 1244, 1986, 2153, 2219, 2330
TaiI ACGT 5 cut(s) 153, 934, 1369, 1420, 1769
TaqI TCGA 5 cut(s) 279, 928, 1143, 1785, 2109
TaqII GACCGA 1 cut(s) 155
TatI WGTACW 3 cut(s) 633, 1480, 1837
TauI GCSGC 2 cut(s) 520, 2035
Tru1I TTAA 7 cut(s) 195, 399, 1127, 1332, 1383, 1434, 1968
Tru9I TTAA 7 cut(s) 195, 399, 1127, 1332, 1383, 1434, 1968
TscAI CASTG 1 cut(s) 2307
TseFI GTSAC 2 cut(s) 338, 533
Tsp45I GTSAC 2 cut(s) 338, 533
TspGWI ACGGA 1 cut(s) 2306
TspRI CASTG 1 cut(s) 2307
Tth111I GACNNNGTC 1 cut(s) 142
Van91I CCANNNNNTGG 4 cut(s) 617, 1079, 1324, 1999
VpaK11BI GGWCC 3 cut(s) 242, 289, 1568
VspI ATTAAT 2 cut(s) 195, 1332
XagI CCTNNNNNAGG 1 cut(s) 2625
XapI RAATTY 6 cut(s) 1940, 2176, 2383, 2449, 2498, 2548
XceI RCATGY 1 cut(s) 853
XmnI GAANNNNTTC 1 cut(s) 1923
ZraI GACGTC 1 cut(s) 151
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.