Rmu_co8217558.1_g000001

zinc finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_co8217558.1
OG0000854
Physical Location & Seq
Reverse (-)
2 .. 590
589 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_co8217558.1_g000001.1.cds

Sequence Viewer

Length: 589 bp
atggaggagaaatcaacgattaggtttcggaacattgaacagctcaagttacacttgttccaccagcataggttgtttatgtgtcctctatgtttggaagggaggaaggtgtttgtatgtgaacaaaagctttatactaaagcccagttgaaacggcatacaagtacaggggactctgtagttgatggaagtgaaagcgaaagaggtggcttcaagggacatcctatgtgtaagttttgcaaaattggattctacggcgagaatgaactgtatgctcacatgactactggccactataagtgtcatttatgttgcaattctggagaacagtatgaatattataagaactatcatgacctcgagttgcacttcagccgaggccattttctatgtgaagatgagtcctgccttgagaaaaagtttgtggtgttccgatccgaggcagaattgaagaagcacagtactatggaacatggggggcgtatgtctcgctccaagcgtaatgctgctctccaattaccaactagcttccgatatggaccaagtaatgaccaagatagtagacgtggaagaagaggtctgccatttc
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

196

Amino Acids

22.99

Weight (kDa)

8.97

Isoelectric Point (pI)

54.19

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 342
AccI GTMKAC 1 cut(s) 562
AclWI GGATC 1 cut(s) 429
AcoI YGGCCR 1 cut(s) 289
AcuI CTGAAG 1 cut(s) 355
AfaI GTAC 2 cut(s) 166, 463
AfiI CCNNNNNNNGG 1 cut(s) 439
AgsI TTSAA 4 cut(s) 38, 151, 214, 451
AjiI CACGTC 1 cut(s) 566
AluBI AGCT 3 cut(s) 43, 130, 528
AluI AGCT 3 cut(s) 43, 130, 528
Alw26I GTCTC 1 cut(s) 492
AlwI GGATC 1 cut(s) 429
Ama87I CYCGRG 1 cut(s) 359
AoxI GGCC 2 cut(s) 289, 379
ApeKI GCWGC 1 cut(s) 506
AspS9I GGNCC 1 cut(s) 539
AvaI CYCGRG 1 cut(s) 359
AvaII GGWCC 1 cut(s) 539
BalI TGGCCA 1 cut(s) 291
BbvI GCAGC 1 cut(s) 493
BccI CCATC 1 cut(s) 179
BceAI ACGGC 2 cut(s) 170, 271
BcoDI GTCTC 1 cut(s) 492
BfaI CTAG 1 cut(s) 525
BfmI CTRYAG 1 cut(s) 177
BisI GCNGC 1 cut(s) 507
BlsI GCNGC 1 cut(s) 508
BmcAI AGTACT 1 cut(s) 463
Bme18I GGWCC 1 cut(s) 539
BmeT110I CYCGRG 1 cut(s) 359
BmgBI CACGTC 1 cut(s) 566
BmgT120I GGNCC 1 cut(s) 539
BmrI ACTGGG 1 cut(s) 139
BmuI ACTGGG 1 cut(s) 139
BpmI CTGGAG 1 cut(s) 342
BpuEI CTTGAG 2 cut(s) 29, 431
BsaJI CCNNGG 2 cut(s) 376, 438
Bsc4I CCNNNNNNNGG 1 cut(s) 439
Bse1I ACTGG 2 cut(s) 145, 292
BseDI CCNNGG 2 cut(s) 376, 438
BseGI GGATG 1 cut(s) 220
BseLI CCNNNNNNNGG 1 cut(s) 439
BseNI ACTGG 2 cut(s) 145, 292
BseRI GAGGAG 1 cut(s) 20
BseXI GCAGC 1 cut(s) 493
BshFI GGCC 2 cut(s) 291, 381
BsiHKCI CYCGRG 1 cut(s) 359
BslFI GGGAC 2 cut(s) 185, 231
BslI CCNNNNNNNGG 1 cut(s) 439
BsmAI GTCTC 1 cut(s) 492
BsmFI GGGAC 2 cut(s) 185, 231
BsnI GGCC 2 cut(s) 291, 381
BsoBI CYCGRG 1 cut(s) 359
Bsp143I GATC 1 cut(s) 434
BspANI GGCC 2 cut(s) 291, 381
BspHI TCATGA 1 cut(s) 352
BspPI GGATC 1 cut(s) 429
BsrI ACTGG 2 cut(s) 145, 292
BssECI CCNNGG 2 cut(s) 376, 438
BssMI GATC 1 cut(s) 434
Bst4CI ACNGT 3 cut(s) 270, 330, 461
Bst6I CTCTTC 1 cut(s) 568
BstF5I GGATG 1 cut(s) 220
BstKTI GATC 1 cut(s) 437
BstMAI GTCTC 1 cut(s) 492
BstMBI GATC 1 cut(s) 434
BstSFI CTRYAG 1 cut(s) 177
BstV1I GCAGC 1 cut(s) 493
BsuRI GGCC 2 cut(s) 291, 381
BtrI CACGTC 1 cut(s) 566
BtsCI GGATG 1 cut(s) 220
CciI TCATGA 1 cut(s) 352
Cfr13I GGNCC 1 cut(s) 539
Csp6I GTAC 2 cut(s) 165, 462
CviAII CATG 3 cut(s) 280, 353, 473
CviJI RGCY 8 cut(s) 43, 130, 143, 210, 291, 375, 381, 528
CviKI_1 RGCY 8 cut(s) 43, 130, 143, 210, 291, 375, 381, 528
CviQI GTAC 2 cut(s) 165, 462
DpnI GATC 1 cut(s) 436
DpnII GATC 1 cut(s) 434
EaeI YGGCCR 1 cut(s) 289
Eam1104I CTCTTC 1 cut(s) 568
EarI CTCTTC 1 cut(s) 568
Eco47I GGWCC 1 cut(s) 539
Eco57I CTGAAG 1 cut(s) 355
Eco88I CYCGRG 1 cut(s) 359
FaeI CATG 3 cut(s) 283, 356, 476
FalI AAGNNNNNCTT 2 cut(s) 38, 70
FaqI GGGAC 2 cut(s) 185, 231
FatI CATG 3 cut(s) 279, 352, 472
FblI GTMKAC 1 cut(s) 562
Fnu4HI GCNGC 1 cut(s) 507
FokI GGATG 1 cut(s) 207
Fsp4HI GCNGC 1 cut(s) 507
FspBI CTAG 1 cut(s) 525
GluI GCNGC 1 cut(s) 507
GsuI CTGGAG 1 cut(s) 342
HaeIII GGCC 2 cut(s) 291, 381
Hin1II CATG 3 cut(s) 283, 356, 476
HindIII AAGCTT 1 cut(s) 128
HinfI GANTC 3 cut(s) 173, 249, 401
Hpy166II GTNNAC 2 cut(s) 122, 563
Hpy188I TCNGA 4 cut(s) 30, 434, 439, 533
Hpy188III TCNNGA 2 cut(s) 321, 353
Hpy8I GTNNAC 2 cut(s) 122, 563
HpyAV CCTTC 2 cut(s) 92, 100
HpyCH4III ACNGT 3 cut(s) 270, 330, 461
HpyCH4IV ACGT 1 cut(s) 565
HpyCH4V TGCA 3 cut(s) 240, 315, 367
HpySE526I ACGT 1 cut(s) 565
Hsp92II CATG 3 cut(s) 283, 356, 476
Kzo9I GATC 1 cut(s) 434
LmnI GCTCC 1 cut(s) 497
LpnPI CCDG 6 cut(s) 77, 153, 158, 273, 306, 418
Lsp1109I GCAGC 1 cut(s) 493
MaeI CTAG 1 cut(s) 525
MaeII ACGT 1 cut(s) 565
MaeIII GTNAC 1 cut(s) 48
MalI GATC 1 cut(s) 436
MboI GATC 1 cut(s) 434
MboII GAAGA 4 cut(s) 407, 463, 582, 585
MlsI TGGCCA 1 cut(s) 291
MluCI AATT 4 cut(s) 243, 316, 446, 515
MluNI TGGCCA 1 cut(s) 291
MlyI GAGTC 2 cut(s) 167, 410
MnlI CCTC 7 cut(s) 96, 96, 197, 368, 371, 433, 569
Mox20I TGGCCA 1 cut(s) 291
MscI TGGCCA 1 cut(s) 291
Msp20I TGGCCA 1 cut(s) 291
NdeII GATC 1 cut(s) 434
NlaIII CATG 3 cut(s) 283, 356, 476
NmeAIII GCCGAG 1 cut(s) 401
PaeR7I CTCGAG 1 cut(s) 359
PagI TCATGA 1 cut(s) 352
PcsI WCGNNNNNNNCGW 1 cut(s) 496
PfeI GAWTC 1 cut(s) 249
PkrI GCNGC 1 cut(s) 508
PleI GAGTC 2 cut(s) 167, 409
PpsI GAGTC 2 cut(s) 167, 409
PsiI TTATAA 1 cut(s) 342
PspPI GGNCC 1 cut(s) 539
PspXI VCTCGAGB 1 cut(s) 359
RsaI GTAC 2 cut(s) 166, 463
RsaNI GTAC 2 cut(s) 165, 462
SatI GCNGC 1 cut(s) 507
Sau3AI GATC 1 cut(s) 434
Sau96I GGNCC 1 cut(s) 539
ScaI AGTACT 1 cut(s) 463
SchI GAGTC 2 cut(s) 167, 410
SfcI CTRYAG 1 cut(s) 177
Sfr274I CTCGAG 1 cut(s) 359
SinI GGWCC 1 cut(s) 539
SlaI CTCGAG 1 cut(s) 359
SmlI CTYRAG 3 cut(s) 44, 359, 410
SmoI CTYRAG 3 cut(s) 44, 359, 410
Sse9I AATT 4 cut(s) 243, 316, 446, 515
SspI AATATT 1 cut(s) 338
SspMI CTAG 1 cut(s) 525
TaaI ACNGT 3 cut(s) 270, 330, 461
TaiI ACGT 1 cut(s) 568
TaqI TCGA 1 cut(s) 360
TasI AATT 4 cut(s) 243, 316, 446, 515
TatI WGTACW 2 cut(s) 164, 461
TfiI GAWTC 1 cut(s) 249
TseI GCWGC 1 cut(s) 506
TspDTI ATGAA 2 cut(s) 279, 348
VpaK11BI GGWCC 1 cut(s) 539
XhoI CTCGAG 1 cut(s) 359
XmiI GTMKAC 1 cut(s) 562
XspI CTAG 1 cut(s) 525
ZrmI AGTACT 1 cut(s) 463
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.