pycom07g03270

zinc finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0000854
Physical Location & Seq
Forward (+)
2725421 .. 2725804
384 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g03270.1

Sequence Viewer

Length: 384 bp
ATGGAAGAAGCTGTGGAGGTGTTGACAAAGGACGGGTACCGTGGTGCCAAAGGAAAATCAAAGCAGTTTGCTAATGAGCACCAGGTGGAGTTAAATTCTCGCACTCAACCTCTAGTACAACTGAGCGGCCAAAAGGATTCCCTGCCAGCTGCTAGTGGATCTAATCCGAATTTAGTGGATGGCGGTGGAGGGAGTAAGCAGCGGAAGAAAACTTCCAAATTTCATAGAGTCCGCTTGGGTGATGCTGCAGCACTTTTAGATCTTGGAAATTCTGATTCTCAGCCAGATGCCGGTGATGAAGGATTAAATGGAGGCAGTAACTCAGCTGGAGGATTGCCTGTCAAAGGTGTGTGGCGGAAAGGAACTCAGAAACTCTTTAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

128

Amino Acids

13.29

Weight (kDa)

9.47

Isoelectric Point (pI)

21.84

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 36
AccB1I GGYRCC 2 cut(s) 36, 44
AccBSI CCGCTC 1 cut(s) 126
AciI CCGC 5 cut(s) 126, 183, 202, 232, 355
AclWI GGATC 1 cut(s) 166
AcoI YGGCCR 1 cut(s) 127
AcsI RAATTY 4 cut(s) 94, 169, 218, 268
AdeI CACNNNGTG 1 cut(s) 85
AfaI GTAC 2 cut(s) 38, 117
AfiI CCNNNNNNNGG 2 cut(s) 290, 344
AjnI CCWGG 1 cut(s) 81
AluBI AGCT 3 cut(s) 11, 149, 326
AluI AGCT 3 cut(s) 11, 149, 326
Alw21I GWGCWC 1 cut(s) 81
AlwI GGATC 1 cut(s) 166
AoxI GGCC 1 cut(s) 127
ApeKI GCWGC 4 cut(s) 149, 199, 245, 248
ApoI RAATTY 4 cut(s) 94, 169, 218, 268
Asp718I GGTACC 1 cut(s) 36
AsuHPI GGTGA 2 cut(s) 251, 305
BaeI ACNNNNGTAYC 2 cut(s) 28, 61
BanI GGYRCC 2 cut(s) 36, 44
Bbv12I GWGCWC 1 cut(s) 81
BbvI GCAGC 4 cut(s) 136, 211, 232, 260
BccI CCATC 1 cut(s) 173
BciT130I CCWGG 1 cut(s) 83
BfaI CTAG 2 cut(s) 113, 153
BfmI CTRYAG 1 cut(s) 246
BglII AGATCT 1 cut(s) 259
BisI GCNGC 5 cut(s) 127, 150, 200, 246, 249
BlsI GCNGC 5 cut(s) 128, 151, 201, 247, 250
Bme1390I CCNGG 1 cut(s) 83
BmiI GGNNCC 2 cut(s) 38, 46
BmrFI CCNGG 1 cut(s) 83
BmsI GCATC 2 cut(s) 232, 277
BpmI CTGGAG 1 cut(s) 348
BsaJI CCNNGG 1 cut(s) 40
Bsc4I CCNNNNNNNGG 2 cut(s) 290, 344
Bse118I RCCGGY 1 cut(s) 290
BseBI CCWGG 1 cut(s) 83
BseDI CCNNGG 1 cut(s) 40
BseGI GGATG 1 cut(s) 184
BseLI CCNNNNNNNGG 2 cut(s) 290, 344
BseMII CTCAG 4 cut(s) 113, 293, 336, 380
BseXI GCAGC 4 cut(s) 136, 211, 232, 260
BshFI GGCC 1 cut(s) 129
BshNI GGYRCC 2 cut(s) 36, 44
BsiHKAI GWGCWC 1 cut(s) 81
BsiSI CCGG 1 cut(s) 291
BslI CCNNNNNNNGG 2 cut(s) 290, 344
BsnI GGCC 1 cut(s) 129
Bsp1286I GDGCHC 1 cut(s) 81
Bsp143I GATC 2 cut(s) 158, 259
BspACI CCGC 5 cut(s) 126, 183, 202, 232, 355
BspANI GGCC 1 cut(s) 129
BspCNI CTCAG 4 cut(s) 114, 292, 335, 379
BspLI GGNNCC 2 cut(s) 38, 46
BspMAI CTGCAG 1 cut(s) 250
BspPI GGATC 1 cut(s) 166
BspT107I GGYRCC 2 cut(s) 36, 44
BsrBI CCGCTC 1 cut(s) 126
BsrFI RCCGGY 1 cut(s) 290
BssAI RCCGGY 1 cut(s) 290
BssECI CCNNGG 1 cut(s) 40
BssMI GATC 2 cut(s) 158, 259
Bst2UI CCWGG 1 cut(s) 83
Bst4CI ACNGT 1 cut(s) 41
BstC8I GCNNGC 1 cut(s) 147
BstDEI CTNAG 4 cut(s) 122, 279, 322, 366
BstDSI CCRYGG 1 cut(s) 40
BstENI CCTNNNNNAGG 1 cut(s) 342
BstF5I GGATG 1 cut(s) 184
BstKTI GATC 2 cut(s) 161, 262
BstMBI GATC 2 cut(s) 158, 259
BstNI CCWGG 1 cut(s) 83
BstSCI CCNGG 1 cut(s) 81
BstSFI CTRYAG 1 cut(s) 246
BstV1I GCAGC 4 cut(s) 136, 211, 232, 260
BstX2I RGATCY 2 cut(s) 158, 259
BstYI RGATCY 2 cut(s) 158, 259
BsuRI GGCC 1 cut(s) 129
BtgI CCRYGG 1 cut(s) 40
BtsCI GGATG 1 cut(s) 184
Cac8I GCNNGC 1 cut(s) 147
Cfr10I RCCGGY 1 cut(s) 290
CsiI ACCWGGT 1 cut(s) 81
Csp6I GTAC 2 cut(s) 37, 116
CviJI RGCY 5 cut(s) 11, 129, 149, 283, 326
CviKI_1 RGCY 5 cut(s) 11, 129, 149, 283, 326
CviQI GTAC 2 cut(s) 37, 116
DdeI CTNAG 4 cut(s) 122, 279, 322, 366
DpnI GATC 2 cut(s) 160, 261
DpnII GATC 2 cut(s) 158, 259
DraIII CACNNNGTG 1 cut(s) 85
EaeI YGGCCR 1 cut(s) 127
EciI GGCGGA 1 cut(s) 370
EcoNI CCTNNNNNAGG 1 cut(s) 342
EcoRII CCWGG 1 cut(s) 81
FaiI YATR 1 cut(s) 225
Fnu4HI GCNGC 5 cut(s) 127, 150, 200, 246, 249
FokI GGATG 1 cut(s) 191
Fsp4HI GCNGC 5 cut(s) 127, 150, 200, 246, 249
FspBI CTAG 2 cut(s) 113, 153
GluI GCNGC 5 cut(s) 127, 150, 200, 246, 249
GsuI CTGGAG 1 cut(s) 348
HaeIII GGCC 1 cut(s) 129
HapII CCGG 1 cut(s) 291
HincII GTYRAC 1 cut(s) 24
HindII GTYRAC 1 cut(s) 24
HinfI GANTC 3 cut(s) 137, 228, 275
HpaII CCGG 1 cut(s) 291
HphI GGTGA 2 cut(s) 251, 305
Hpy166II GTNNAC 1 cut(s) 24
Hpy188I TCNGA 3 cut(s) 168, 274, 369
Hpy8I GTNNAC 1 cut(s) 24
HpyAV CCTTC 1 cut(s) 293
HpyCH4III ACNGT 1 cut(s) 41
HpyCH4V TGCA 1 cut(s) 248
HpyF3I CTNAG 4 cut(s) 122, 279, 322, 366
KpnI GGTACC 1 cut(s) 40
Kzo9I GATC 2 cut(s) 158, 259
LpnPI CCDG 8 cut(s) 68, 95, 155, 159, 297, 304, 312, 351
Lsp1109I GCAGC 4 cut(s) 136, 211, 232, 260
LweI GCATC 2 cut(s) 232, 277
MabI ACCWGGT 1 cut(s) 81
MaeI CTAG 2 cut(s) 113, 153
MaeIII GTNAC 1 cut(s) 317
MalI GATC 2 cut(s) 160, 261
MbiI CCGCTC 1 cut(s) 126
MboI GATC 2 cut(s) 158, 259
MboII GAAGA 2 cut(s) 17, 217
MflI RGATCY 2 cut(s) 158, 259
MhlI GDGCHC 1 cut(s) 81
MluCI AATT 4 cut(s) 94, 169, 218, 268
MlyI GAGTC 1 cut(s) 237
MnlI CCTC 5 cut(s) 10, 120, 182, 305, 323
MseI TTAA 2 cut(s) 92, 305
MspA1I CMGCKG 3 cut(s) 149, 202, 326
MspI CCGG 1 cut(s) 291
MspR9I CCNGG 1 cut(s) 83
MvaI CCWGG 1 cut(s) 83
NdeII GATC 2 cut(s) 158, 259
NlaIV GGNNCC 2 cut(s) 38, 46
PfeI GAWTC 2 cut(s) 137, 275
PkrI GCNGC 5 cut(s) 128, 151, 201, 247, 250
PleI GAGTC 1 cut(s) 236
PpsI GAGTC 1 cut(s) 236
Psp6I CCWGG 1 cut(s) 81
PspGI CCWGG 1 cut(s) 81
PspN4I GGNNCC 2 cut(s) 38, 46
PstI CTGCAG 1 cut(s) 250
PsuI RGATCY 2 cut(s) 158, 259
PvuII CAGCTG 2 cut(s) 149, 326
RsaI GTAC 2 cut(s) 38, 117
RsaNI GTAC 2 cut(s) 37, 116
SaqAI TTAA 2 cut(s) 92, 305
SatI GCNGC 5 cut(s) 127, 150, 200, 246, 249
Sau3AI GATC 2 cut(s) 158, 259
SchI GAGTC 1 cut(s) 237
ScrFI CCNGG 1 cut(s) 83
SduI GDGCHC 1 cut(s) 81
SetI ASST 7 cut(s) 13, 21, 87, 112, 151, 328, 349
SexAI ACCWGGT 1 cut(s) 81
SfaNI GCATC 2 cut(s) 232, 277
SfcI CTRYAG 1 cut(s) 246
Sse9I AATT 4 cut(s) 94, 169, 218, 268
SsiI CCGC 5 cut(s) 126, 183, 202, 232, 355
SspMI CTAG 2 cut(s) 113, 153
StyD4I CCNGG 1 cut(s) 81
TaaI ACNGT 1 cut(s) 41
TasI AATT 4 cut(s) 94, 169, 218, 268
TatI WGTACW 1 cut(s) 115
TauI GCSGC 1 cut(s) 129
TfiI GAWTC 2 cut(s) 137, 275
Tru1I TTAA 2 cut(s) 92, 305
Tru9I TTAA 2 cut(s) 92, 305
TseI GCWGC 4 cut(s) 149, 199, 245, 248
TspDTI ATGAA 2 cut(s) 212, 312
XagI CCTNNNNNAGG 1 cut(s) 342
XapI RAATTY 4 cut(s) 94, 169, 218, 268
XspI CTAG 2 cut(s) 113, 153
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.