zinc finger
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 678 |
| AarI | CACCTGC | 1 cut(s) | 92 |
| AasI | GACNNNNNNGTC | 1 cut(s) | 1696 |
| AatII | GACGTC | 1 cut(s) | 1400 |
| Acc36I | ACCTGC | 1 cut(s) | 92 |
| Acc65I | GGTACC | 2 cut(s) | 243, 1110 |
| AccB1I | GGYRCC | 2 cut(s) | 243, 1110 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 1601 |
| AccI | GTMKAC | 1 cut(s) | 1926 |
| AccII | CGCG | 1 cut(s) | 1917 |
| AciI | CCGC | 7 cut(s) | 118, 278, 1291, 1361, 1481, 1915, 2292 |
| AclWI | GGATC | 7 cut(s) | 241, 288, 765, 778, 989, 1040, 1242 |
| AcoI | YGGCCR | 6 cut(s) | 61, 1352, 1472, 1592, 1727, 2293 |
| AcsI | RAATTY | 1 cut(s) | 2200 |
| AcuI | CTGAAG | 2 cut(s) | 691, 850 |
| AcyI | GRCGYC | 2 cut(s) | 1397, 1637 |
| AfaI | GTAC | 6 cut(s) | 245, 502, 799, 1112, 1507, 1971 |
| AfiI | CCNNNNNNNGG | 8 cut(s) | 39, 40, 1297, 1361, 1481, 1601, 2053, 2270 |
| AflII | CTTAAG | 1 cut(s) | 1072 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 624 |
| AhlI | ACTAGT | 3 cut(s) | 1382, 1502, 1676 |
| AjnI | CCWGG | 3 cut(s) | 33, 1192, 2268 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 1142, 1174 |
| AleI | CACNNNNGTG | 1 cut(s) | 86 |
| Alw21I | GWGCWC | 1 cut(s) | 1851 |
| Alw26I | GTCTC | 6 cut(s) | 171, 192, 828, 956, 1826, 2157 |
| Alw44I | GTGCAC | 1 cut(s) | 1847 |
| AlwI | GGATC | 7 cut(s) | 241, 288, 765, 778, 989, 1040, 1242 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 1601, 1693 |
| Ama87I | CYCGRG | 1 cut(s) | 1657 |
| ApaLI | GTGCAC | 1 cut(s) | 1847 |
| ApoI | RAATTY | 1 cut(s) | 2200 |
| Asp718I | GGTACC | 2 cut(s) | 243, 1110 |
| AspLEI | GCGC | 2 cut(s) | 1156, 1919 |
| AspS9I | GGNCC | 3 cut(s) | 115, 875, 2164 |
| AsuHPI | GGTGA | 1 cut(s) | 154 |
| AvaI | CYCGRG | 1 cut(s) | 1657 |
| AvaII | GGWCC | 3 cut(s) | 115, 875, 2164 |
| BaeGI | GKGCMC | 2 cut(s) | 1851, 2069 |
| BalI | TGGCCA | 5 cut(s) | 63, 1354, 1474, 1594, 1729 |
| BamHI | GGATCC | 1 cut(s) | 770 |
| BanI | GGYRCC | 2 cut(s) | 243, 1110 |
| BbsI | GAAGAC | 1 cut(s) | 1944 |
| Bbv12I | GWGCWC | 1 cut(s) | 1851 |
| BbvCI | CCTCAGC | 1 cut(s) | 1432 |
| BceAI | ACGGC | 4 cut(s) | 105, 506, 607, 1504 |
| BcgI | CGANNNNNNTGC | 1 cut(s) | 30 |
| BciT130I | CCWGG | 3 cut(s) | 35, 1194, 2270 |
| BclI | TGATCA | 2 cut(s) | 886, 943 |
| BcoDI | GTCTC | 6 cut(s) | 171, 192, 828, 956, 1826, 2157 |
| BcuI | ACTAGT | 3 cut(s) | 1382, 1502, 1676 |
| BfmI | CTRYAG | 4 cut(s) | 513, 1198, 1808, 2220 |
| BfrI | CTTAAG | 1 cut(s) | 1072 |
| BfuAI | ACCTGC | 1 cut(s) | 92 |
| BglII | AGATCT | 1 cut(s) | 2236 |
| BmcAI | AGTACT | 1 cut(s) | 799 |
| Bme1390I | CCNGG | 3 cut(s) | 35, 1194, 2270 |
| Bme18I | GGWCC | 3 cut(s) | 115, 875, 2164 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 624 |
| BmeT110I | CYCGRG | 1 cut(s) | 1657 |
| BmgT120I | GGNCC | 3 cut(s) | 115, 875, 2164 |
| BmiI | GGNNCC | 3 cut(s) | 245, 772, 1112 |
| BmrFI | CCNGG | 3 cut(s) | 35, 1194, 2270 |
| BmsI | GCATC | 3 cut(s) | 138, 1109, 1506 |
| BpiI | GAAGAC | 1 cut(s) | 1944 |
| BpmI | CTGGAG | 2 cut(s) | 678, 833 |
| Bpu10I | CCTNAGC | 1 cut(s) | 1432 |
| BpuEI | CTTGAG | 4 cut(s) | 365, 716, 767, 1800 |
| BsaHI | GRCGYC | 2 cut(s) | 1397, 1637 |
| BsaI | GGTCTC | 1 cut(s) | 2157 |
| Bsc4I | CCNNNNNNNGG | 8 cut(s) | 39, 40, 1297, 1361, 1481, 1601, 2053, 2270 |
| Bse1I | ACTGG | 3 cut(s) | 628, 850, 2228 |
| Bse3DI | GCAATG | 1 cut(s) | 2131 |
| BseBI | CCWGG | 3 cut(s) | 35, 1194, 2270 |
| BseGI | GGATG | 6 cut(s) | 76, 556, 1431, 1551, 2146, 2153 |
| BseLI | CCNNNNNNNGG | 8 cut(s) | 39, 40, 1297, 1361, 1481, 1601, 2053, 2270 |
| BseMI | GCAATG | 1 cut(s) | 2131 |
| BseMII | CTCAG | 8 cut(s) | 1406, 1446, 1526, 1566, 1700, 2067, 2168, 2254 |
| BseNI | ACTGG | 3 cut(s) | 628, 850, 2228 |
| BseRI | GAGGAG | 1 cut(s) | 356 |
| BseSI | GKGCMC | 2 cut(s) | 1851, 2069 |
| BseYI | CCCAGC | 1 cut(s) | 1601 |
| BsgI | GTGCAG | 3 cut(s) | 36, 1683, 2151 |
| Bsh1236I | CGCG | 1 cut(s) | 1917 |
| BshNI | GGYRCC | 2 cut(s) | 243, 1110 |
| BsiHKAI | GWGCWC | 1 cut(s) | 1851 |
| BsiHKCI | CYCGRG | 1 cut(s) | 1657 |
| BslFI | GGGAC | 2 cut(s) | 521, 567 |
| BslI | CCNNNNNNNGG | 8 cut(s) | 39, 40, 1297, 1361, 1481, 1601, 2053, 2270 |
| BsmAI | GTCTC | 6 cut(s) | 171, 192, 828, 956, 1826, 2157 |
| BsmFI | GGGAC | 2 cut(s) | 521, 567 |
| Bso31I | GGTCTC | 1 cut(s) | 2157 |
| BsoBI | CYCGRG | 1 cut(s) | 1657 |
| Bsp1286I | GDGCHC | 2 cut(s) | 1851, 2069 |
| Bsp1407I | TGTACA | 1 cut(s) | 1969 |
| Bsp19I | CCATGG | 2 cut(s) | 1260, 1640 |
| BspACI | CCGC | 7 cut(s) | 118, 278, 1291, 1361, 1481, 1915, 2292 |
| BspCNI | CTCAG | 8 cut(s) | 1405, 1445, 1525, 1565, 1699, 2068, 2167, 2253 |
| BspFNI | CGCG | 1 cut(s) | 1917 |
| BspHI | TCATGA | 1 cut(s) | 688 |
| BspLI | GGNNCC | 3 cut(s) | 245, 772, 1112 |
| BspMAI | CTGCAG | 3 cut(s) | 1202, 1812, 2224 |
| BspMI | ACCTGC | 1 cut(s) | 92 |
| BspPI | GGATC | 7 cut(s) | 241, 288, 765, 778, 989, 1040, 1242 |
| BspT107I | GGYRCC | 2 cut(s) | 243, 1110 |
| BspTI | CTTAAG | 1 cut(s) | 1072 |
| BspTNI | GGTCTC | 1 cut(s) | 2157 |
| BsrDI | GCAATG | 1 cut(s) | 2131 |
| BsrGI | TGTACA | 1 cut(s) | 1969 |
| BsrI | ACTGG | 3 cut(s) | 628, 850, 2228 |
| BssNI | GRCGYC | 2 cut(s) | 1397, 1637 |
| BssT1I | CCWWGG | 5 cut(s) | 165, 998, 1260, 1640, 1899 |
| Bst2UI | CCWGG | 3 cut(s) | 35, 1194, 2270 |
| Bst4CI | ACNGT | 8 cut(s) | 483, 606, 666, 797, 1453, 1974, 2064, 2114 |
| Bst6I | CTCTTC | 3 cut(s) | 904, 1174, 2130 |
| BstACI | GRCGYC | 2 cut(s) | 1397, 1637 |
| BstAFI | CTTAAG | 1 cut(s) | 1072 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 23 |
| BstAUI | TGTACA | 1 cut(s) | 1969 |
| BstDSI | CCRYGG | 3 cut(s) | 1260, 1520, 1640 |
| BstF5I | GGATG | 6 cut(s) | 76, 556, 1431, 1551, 2146, 2153 |
| BstFNI | CGCG | 1 cut(s) | 1917 |
| BstHHI | GCGC | 2 cut(s) | 1156, 1919 |
| BstMAI | GTCTC | 6 cut(s) | 171, 192, 828, 956, 1826, 2157 |
| BstNI | CCWGG | 3 cut(s) | 35, 1194, 2270 |
| BstNSI | RCATGY | 1 cut(s) | 1370 |
| BstSCI | CCNGG | 3 cut(s) | 33, 1192, 2268 |
| BstSFI | CTRYAG | 4 cut(s) | 513, 1198, 1808, 2220 |
| BstSLI | GKGCMC | 2 cut(s) | 1851, 2069 |
| BstUI | CGCG | 1 cut(s) | 1917 |
| BstV2I | GAAGAC | 1 cut(s) | 1944 |
| BstX2I | RGATCY | 2 cut(s) | 770, 2236 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 88 |
| BstYI | RGATCY | 2 cut(s) | 770, 2236 |
| BtgI | CCRYGG | 3 cut(s) | 1260, 1520, 1640 |
| BtsCI | GGATG | 6 cut(s) | 76, 556, 1431, 1551, 2146, 2153 |
| BtsI | GCAGTG | 3 cut(s) | 24, 328, 1947 |
| BtsIMutI | CAGTG | 3 cut(s) | 24, 328, 1947 |
| BveI | ACCTGC | 1 cut(s) | 92 |
| CaiI | CAGNNNCTG | 2 cut(s) | 1601, 1693 |
| CciI | TCATGA | 1 cut(s) | 688 |
| CfoI | GCGC | 2 cut(s) | 1156, 1919 |
| Cfr13I | GGNCC | 3 cut(s) | 115, 875, 2164 |
| CseI | GACGC | 2 cut(s) | 1645, 1699 |
| Csp6I | GTAC | 6 cut(s) | 244, 501, 798, 1111, 1506, 1970 |
| CviQI | GTAC | 6 cut(s) | 244, 501, 798, 1111, 1506, 1970 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 1696 |
| DriI | GACNNNNNGTC | 1 cut(s) | 624 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 1696 |
| EaeI | YGGCCR | 6 cut(s) | 61, 1352, 1472, 1592, 1727, 2293 |
| Eam1104I | CTCTTC | 3 cut(s) | 904, 1174, 2130 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 624 |
| EarI | CTCTTC | 3 cut(s) | 904, 1174, 2130 |
| Eco130I | CCWWGG | 5 cut(s) | 165, 998, 1260, 1640, 1899 |
| Eco147I | AGGCCT | 1 cut(s) | 2102 |
| Eco31I | GGTCTC | 1 cut(s) | 2157 |
| Eco47I | GGWCC | 3 cut(s) | 115, 875, 2164 |
| Eco57I | CTGAAG | 2 cut(s) | 691, 850 |
| Eco88I | CYCGRG | 1 cut(s) | 1657 |
| EcoRI | GAATTC | 1 cut(s) | 2200 |
| EcoRII | CCWGG | 3 cut(s) | 33, 1192, 2268 |
| EcoT14I | CCWWGG | 5 cut(s) | 165, 998, 1260, 1640, 1899 |
| ErhI | CCWWGG | 5 cut(s) | 165, 998, 1260, 1640, 1899 |
| FalI | AAGNNNNNCTT | 6 cut(s) | 374, 406, 1066, 1098, 1748, 1780 |
| FaqI | GGGAC | 2 cut(s) | 521, 567 |
| FauI | CCCGC | 2 cut(s) | 1354, 1474 |
| FbaI | TGATCA | 2 cut(s) | 886, 943 |
| FblI | GTMKAC | 1 cut(s) | 1926 |
| FokI | GGATG | 6 cut(s) | 83, 543, 1438, 1558, 2153, 2160 |
| GlaI | GCGC | 2 cut(s) | 1155, 1918 |
| GsaI | CCCAGC | 1 cut(s) | 1605 |
| GsuI | CTGGAG | 2 cut(s) | 678, 833 |
| HgaI | GACGC | 2 cut(s) | 1645, 1699 |
| HhaI | GCGC | 2 cut(s) | 1156, 1919 |
| Hin1I | GRCGYC | 2 cut(s) | 1397, 1637 |
| Hin6I | GCGC | 2 cut(s) | 1154, 1917 |
| HinP1I | GCGC | 2 cut(s) | 1154, 1917 |
| HincII | GTYRAC | 2 cut(s) | 1211, 1927 |
| HindII | GTYRAC | 2 cut(s) | 1211, 1927 |
| HindIII | AAGCTT | 1 cut(s) | 464 |
| HphI | GGTGA | 1 cut(s) | 154 |
| Hpy166II | GTNNAC | 5 cut(s) | 458, 1211, 1849, 1927, 1993 |
| Hpy188III | TCNNGA | 5 cut(s) | 657, 689, 890, 1313, 1829 |
| Hpy8I | GTNNAC | 5 cut(s) | 458, 1211, 1849, 1927, 1993 |
| Hpy99I | CGWCG | 2 cut(s) | 155, 355 |
| HpyAV | CCTTC | 5 cut(s) | 428, 436, 945, 1092, 1124 |
| HpyCH4III | ACNGT | 8 cut(s) | 483, 606, 666, 797, 1453, 1974, 2064, 2114 |
| HpyCH4IV | ACGT | 2 cut(s) | 150, 1397 |
| HpySE526I | ACGT | 2 cut(s) | 150, 1397 |
| Hsp92I | GRCGYC | 2 cut(s) | 1397, 1637 |
| HspAI | GCGC | 2 cut(s) | 1154, 1917 |
| KpnI | GGTACC | 2 cut(s) | 247, 1114 |
| Ksp22I | TGATCA | 2 cut(s) | 886, 943 |
| LmnI | GCTCC | 3 cut(s) | 83, 833, 1805 |
| LweI | GCATC | 3 cut(s) | 138, 1109, 1506 |
| MaeII | ACGT | 2 cut(s) | 150, 1397 |
| MaeIII | GTNAC | 8 cut(s) | 160, 329, 626, 852, 1037, 1438, 1558, 2034 |
| MboII | GAAGA | 9 cut(s) | 743, 799, 918, 921, 1095, 1161, 1949, 2147, 2209 |
| MfeI | CAATTG | 1 cut(s) | 1157 |
| MflI | RGATCY | 2 cut(s) | 770, 2236 |
| MhlI | GDGCHC | 2 cut(s) | 1851, 2069 |
| MlsI | TGGCCA | 5 cut(s) | 63, 1354, 1474, 1594, 1729 |
| MluCI | AATT | 9 cut(s) | 579, 652, 682, 782, 1157, 2011, 2094, 2200, 2245 |
| MluNI | TGGCCA | 5 cut(s) | 63, 1354, 1474, 1594, 1729 |
| MlyI | GAGTC | 5 cut(s) | 152, 503, 746, 1076, 1160 |
| MmeI | TCCRAC | 1 cut(s) | 171 |
| Mox20I | TGGCCA | 5 cut(s) | 63, 1354, 1474, 1594, 1729 |
| MscI | TGGCCA | 5 cut(s) | 63, 1354, 1474, 1594, 1729 |
| MseI | TTAA | 4 cut(s) | 1056, 1073, 1956, 2010 |
| MslI | CAYNNNNRTG | 4 cut(s) | 69, 86, 645, 2000 |
| Msp20I | TGGCCA | 5 cut(s) | 63, 1354, 1474, 1594, 1729 |
| MspA1I | CMGCKG | 4 cut(s) | 1361, 1481, 1601, 1922 |
| MspCI | CTTAAG | 1 cut(s) | 1072 |
| MspR9I | CCNGG | 3 cut(s) | 35, 1194, 2270 |
| MunI | CAATTG | 1 cut(s) | 1157 |
| MvaI | CCWGG | 3 cut(s) | 35, 1194, 2270 |
| MvnI | CGCG | 1 cut(s) | 1917 |
| NcoI | CCATGG | 2 cut(s) | 1260, 1640 |
| NlaIV | GGNNCC | 3 cut(s) | 245, 772, 1112 |
| NmeAIII | GCCGAG | 1 cut(s) | 737 |
| NmuCI | GTSAC | 4 cut(s) | 160, 329, 626, 1037 |
| NspI | RCATGY | 1 cut(s) | 1370 |
| OliI | CACNNNNGTG | 1 cut(s) | 86 |
| PagI | TCATGA | 1 cut(s) | 688 |
| PaqCI | CACCTGC | 1 cut(s) | 92 |
| PceI | AGGCCT | 1 cut(s) | 2102 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 832 |
| PfeI | GAWTC | 7 cut(s) | 355, 585, 1019, 1091, 1229, 1825, 2177 |
| PflFI | GACNNNGTC | 1 cut(s) | 142 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 1601 |
| PleI | GAGTC | 5 cut(s) | 151, 503, 745, 1075, 1160 |
| PpsI | GAGTC | 5 cut(s) | 151, 503, 745, 1075, 1160 |
| PsiI | TTATAA | 1 cut(s) | 678 |
| Psp6I | CCWGG | 3 cut(s) | 33, 1192, 2268 |
| PspFI | CCCAGC | 1 cut(s) | 1601 |
| PspGI | CCWGG | 3 cut(s) | 33, 1192, 2268 |
| PspN4I | GGNNCC | 3 cut(s) | 245, 772, 1112 |
| PspPI | GGNCC | 3 cut(s) | 115, 875, 2164 |
| PstI | CTGCAG | 3 cut(s) | 1202, 1812, 2224 |
| PstNI | CAGNNNCTG | 2 cut(s) | 1601, 1693 |
| PsuI | RGATCY | 2 cut(s) | 770, 2236 |
| PsyI | GACNNNGTC | 1 cut(s) | 142 |
| PvuII | CAGCTG | 2 cut(s) | 1601, 1922 |
| RsaI | GTAC | 6 cut(s) | 245, 502, 799, 1112, 1507, 1971 |
| RsaNI | GTAC | 6 cut(s) | 244, 501, 798, 1111, 1506, 1970 |
| RseI | CAYNNNNRTG | 4 cut(s) | 69, 86, 645, 2000 |
| SalI | GTCGAC | 1 cut(s) | 1925 |
| SaqAI | TTAA | 4 cut(s) | 1056, 1073, 1956, 2010 |
| Sau96I | GGNCC | 3 cut(s) | 115, 875, 2164 |
| SbfI | CCTGCAGG | 1 cut(s) | 1812 |
| ScaI | AGTACT | 1 cut(s) | 799 |
| SchI | GAGTC | 5 cut(s) | 152, 503, 746, 1076, 1160 |
| ScrFI | CCNGG | 3 cut(s) | 35, 1194, 2270 |
| SdaI | CCTGCAGG | 1 cut(s) | 1812 |
| SduI | GDGCHC | 2 cut(s) | 1851, 2069 |
| SfaNI | GCATC | 3 cut(s) | 138, 1109, 1506 |
| SfcI | CTRYAG | 4 cut(s) | 513, 1198, 1808, 2220 |
| SinI | GGWCC | 3 cut(s) | 115, 875, 2164 |
| SmiMI | CAYNNNNRTG | 4 cut(s) | 69, 86, 645, 2000 |
| SmlI | CTYRAG | 5 cut(s) | 380, 695, 746, 1072, 1815 |
| SmoI | CTYRAG | 5 cut(s) | 380, 695, 746, 1072, 1815 |
| SpeI | ACTAGT | 3 cut(s) | 1382, 1502, 1676 |
| Sse8387I | CCTGCAGG | 1 cut(s) | 1812 |
| Sse9I | AATT | 9 cut(s) | 579, 652, 682, 782, 1157, 2011, 2094, 2200, 2245 |
| SseBI | AGGCCT | 1 cut(s) | 2102 |
| SsiI | CCGC | 7 cut(s) | 118, 278, 1291, 1361, 1481, 1915, 2292 |
| SspI | AATATT | 1 cut(s) | 674 |
| StuI | AGGCCT | 1 cut(s) | 2102 |
| StyD4I | CCNGG | 3 cut(s) | 33, 1192, 2268 |
| StyI | CCWWGG | 5 cut(s) | 165, 998, 1260, 1640, 1899 |
| TaaI | ACNGT | 8 cut(s) | 483, 606, 666, 797, 1453, 1974, 2064, 2114 |
| TaiI | ACGT | 2 cut(s) | 153, 1400 |
| TaqI | TCGA | 5 cut(s) | 270, 350, 1828, 1926, 1932 |
| TaqII | GACCGA | 1 cut(s) | 155 |
| TasI | AATT | 9 cut(s) | 579, 652, 682, 782, 1157, 2011, 2094, 2200, 2245 |
| TatI | WGTACW | 4 cut(s) | 500, 797, 1505, 1969 |
| TauI | GCSGC | 1 cut(s) | 2295 |
| TfiI | GAWTC | 7 cut(s) | 355, 585, 1019, 1091, 1229, 1825, 2177 |
| Tru1I | TTAA | 4 cut(s) | 1056, 1073, 1956, 2010 |
| Tru9I | TTAA | 4 cut(s) | 1056, 1073, 1956, 2010 |
| TscAI | CASTG | 3 cut(s) | 24, 328, 1954 |
| TseFI | GTSAC | 4 cut(s) | 160, 329, 626, 1037 |
| Tsp45I | GTSAC | 4 cut(s) | 160, 329, 626, 1037 |
| TspDTI | ATGAA | 7 cut(s) | 94, 306, 615, 684, 1377, 1671, 1950 |
| TspGWI | ACGGA | 2 cut(s) | 89, 1265 |
| TspRI | CASTG | 3 cut(s) | 24, 328, 1954 |
| Tth111I | GACNNNGTC | 1 cut(s) | 142 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 1601 |
| Vha464I | CTTAAG | 1 cut(s) | 1072 |
| VneI | GTGCAC | 1 cut(s) | 1847 |
| VpaK11BI | GGWCC | 3 cut(s) | 115, 875, 2164 |
| XapI | RAATTY | 1 cut(s) | 2200 |
| XceI | RCATGY | 1 cut(s) | 1370 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 2303 |
| XmiI | GTMKAC | 1 cut(s) | 1926 |
| ZraI | GACGTC | 1 cut(s) | 1398 |
| ZrmI | AGTACT | 1 cut(s) | 799 |