AT4G32100

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0001875
Physical Location & Seq
Reverse (-)
15511757 .. 15512218
462 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G32100.1

Sequence Viewer

Length: 369 bp
ATGGCAACAAACGCTTGCAAATTCCTTTGCCTAGTTCTCTTATTTGCCTTTGTCACCCAAGGGTATGGGGATGATTCTTATTCTCTCGAGAGCTTATCTGTGATACAATCGAAGACCGGGAACATGGTTGAGAACAAACCGGAGTGGGAAGTGAAAGTGCTGAATTCTAGTCCCTGCTATTTCACTCACACAACGTTGTCATGTGTCCGTTTCAAATCGGTCACACCCATCGACAGTAAGGTTCTATCTAAATCCGGTGACACGTGTCTCCTCGGCAACGGCGACTCTATCCATGATATCAGTTTCAAATACGTATGGGACACAAGCTTCGACCTCAAGGTTGTCGACGGCTACATCGCTTGCTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

122

Amino Acids

13.47

Weight (kDa)

5.17

Isoelectric Point (pI)

22.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPD1_C PF24068 29 - 116 2e-21 Tapetum determinant 1, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 345
AclI AACGTT 1 cut(s) 194
AcsI RAATTY 2 cut(s) 20, 163
AcvI CACGTG 1 cut(s) 264
AflIII ACRYGT 2 cut(s) 261, 263
AgsI TTSAA 2 cut(s) 214, 307
AluBI AGCT 2 cut(s) 93, 327
AluI AGCT 2 cut(s) 93, 327
Alw26I GTCTC 1 cut(s) 272
Ama87I CYCGRG 1 cut(s) 86
ApoI RAATTY 2 cut(s) 20, 163
ArsI GACNNNNNNTTYG 2 cut(s) 311, 343
AsuC2I CCSGG 1 cut(s) 118
AsuHPI GGTGA 2 cut(s) 46, 269
AvaI CYCGRG 1 cut(s) 86
BbrPI CACGTG 1 cut(s) 264
BbsI GAAGAC 1 cut(s) 119
BccI CCATC 1 cut(s) 236
BceAI ACGGC 2 cut(s) 295, 364
BcnI CCSGG 1 cut(s) 118
BcoDI GTCTC 1 cut(s) 272
BfaI CTAG 2 cut(s) 32, 168
Bme1390I CCNGG 1 cut(s) 118
BmeT110I CYCGRG 1 cut(s) 86
BmrFI CCNGG 1 cut(s) 118
BoxI GACNNNNGTC 1 cut(s) 264
BpiI GAAGAC 1 cut(s) 119
BpuEI CTTGAG 1 cut(s) 320
BpuMI CCSGG 1 cut(s) 118
BsaAI YACGTR 2 cut(s) 264, 313
BsaJI CCNNGG 2 cut(s) 58, 271
BsaWI WCCGGW 2 cut(s) 139, 254
BseDI CCNNGG 2 cut(s) 58, 271
BseGI GGATG 1 cut(s) 76
BseRI GAGGAG 1 cut(s) 260
BsiHKCI CYCGRG 1 cut(s) 86
BsiSI CCGG 3 cut(s) 117, 140, 255
BslFI GGGAC 2 cut(s) 156, 332
BsmAI GTCTC 1 cut(s) 272
BsmFI GGGAC 2 cut(s) 156, 332
BsoBI CYCGRG 1 cut(s) 86
BssECI CCNNGG 2 cut(s) 58, 271
BssT1I CCWWGG 1 cut(s) 58
Bst4CI ACNGT 1 cut(s) 236
BstBAI YACGTR 2 cut(s) 264, 313
BstC8I GCNNGC 2 cut(s) 16, 361
BstF5I GGATG 1 cut(s) 76
BstMAI GTCTC 1 cut(s) 272
BstMWI GCNNNNNNNGC 1 cut(s) 11
BstPAI GACNNNNGTC 1 cut(s) 264
BstSCI CCNGG 1 cut(s) 116
BstSNI TACGTA 1 cut(s) 313
BstV2I GAAGAC 1 cut(s) 119
BstXI CCANNNNNNTGG 1 cut(s) 65
BtgZI GCGATG 1 cut(s) 340
BtsCI GGATG 1 cut(s) 76
Cac8I GCNNGC 2 cut(s) 16, 361
CviAII CATG 3 cut(s) 124, 201, 293
CviJI RGCY 3 cut(s) 93, 327, 351
CviKI_1 RGCY 3 cut(s) 93, 327, 351
Eco105I TACGTA 1 cut(s) 313
Eco130I CCWWGG 1 cut(s) 58
Eco32I GATATC 1 cut(s) 298
Eco72I CACGTG 1 cut(s) 264
Eco88I CYCGRG 1 cut(s) 86
EcoRI GAATTC 1 cut(s) 163
EcoRV GATATC 1 cut(s) 298
EcoT14I CCWWGG 1 cut(s) 58
ErhI CCWWGG 1 cut(s) 58
FaeI CATG 3 cut(s) 127, 204, 296
FaiI YATR 5 cut(s) 66, 125, 202, 294, 316
FaqI GGGAC 2 cut(s) 156, 332
FatI CATG 3 cut(s) 123, 200, 292
FblI GTMKAC 1 cut(s) 345
FokI GGATG 1 cut(s) 83
FspBI CTAG 2 cut(s) 32, 168
HapII CCGG 3 cut(s) 117, 140, 255
Hin1II CATG 3 cut(s) 127, 204, 296
HincII GTYRAC 1 cut(s) 346
HindII GTYRAC 1 cut(s) 346
HindIII AAGCTT 1 cut(s) 325
HinfI GANTC 2 cut(s) 74, 284
HpaII CCGG 3 cut(s) 117, 140, 255
HphI GGTGA 2 cut(s) 46, 269
Hpy166II GTNNAC 1 cut(s) 346
Hpy188III TCNNGA 2 cut(s) 86, 88
Hpy8I GTNNAC 1 cut(s) 346
Hpy99I CGWCG 1 cut(s) 350
HpyCH4III ACNGT 1 cut(s) 236
HpyCH4IV ACGT 3 cut(s) 194, 263, 312
HpyCH4V TGCA 1 cut(s) 18
HpyF10VI GCNNNNNNNGC 1 cut(s) 11
HpySE526I ACGT 3 cut(s) 194, 263, 312
Hsp92II CATG 3 cut(s) 127, 204, 296
LpnPI CCDG 4 cut(s) 130, 153, 187, 268
MaeI CTAG 2 cut(s) 32, 168
MaeII ACGT 3 cut(s) 194, 263, 312
MaeIII GTNAC 3 cut(s) 52, 220, 257
MboII GAAGA 1 cut(s) 124
MluCI AATT 2 cut(s) 20, 163
MlyI GAGTC 1 cut(s) 278
MnlI CCTC 2 cut(s) 281, 344
MseI TTAA 1 cut(s) 367
MspI CCGG 3 cut(s) 117, 140, 255
MspR9I CCNGG 1 cut(s) 118
MwoI GCNNNNNNNGC 1 cut(s) 11
NciI CCSGG 1 cut(s) 118
NlaIII CATG 3 cut(s) 127, 204, 296
NmeAIII GCCGAG 1 cut(s) 252
NmuCI GTSAC 3 cut(s) 52, 220, 257
PaeR7I CTCGAG 1 cut(s) 86
PcsI WCGNNNNNNNCGW 1 cut(s) 279
PfeI GAWTC 1 cut(s) 74
PleI GAGTC 1 cut(s) 278
PmaCI CACGTG 1 cut(s) 264
PmlI CACGTG 1 cut(s) 264
PpsI GAGTC 1 cut(s) 278
Ppu21I YACGTR 2 cut(s) 264, 313
PshAI GACNNNNGTC 1 cut(s) 264
Psp1406I AACGTT 1 cut(s) 194
PspCI CACGTG 1 cut(s) 264
SalI GTCGAC 1 cut(s) 344
SaqAI TTAA 1 cut(s) 367
SchI GAGTC 1 cut(s) 278
ScrFI CCNGG 1 cut(s) 118
SetI ASST 8 cut(s) 95, 197, 243, 266, 315, 329, 336, 342
Sfr274I CTCGAG 1 cut(s) 86
SlaI CTCGAG 1 cut(s) 86
SmlI CTYRAG 2 cut(s) 86, 335
SmoI CTYRAG 2 cut(s) 86, 335
SnaBI TACGTA 1 cut(s) 313
Sse9I AATT 2 cut(s) 20, 163
SspMI CTAG 2 cut(s) 32, 168
StyD4I CCNGG 1 cut(s) 116
StyI CCWWGG 1 cut(s) 58
TaaI ACNGT 1 cut(s) 236
TaiI ACGT 3 cut(s) 197, 266, 315
TaqI TCGA 5 cut(s) 87, 110, 231, 330, 345
TaqII GACCGA 1 cut(s) 208
TasI AATT 2 cut(s) 20, 163
TfiI GAWTC 1 cut(s) 74
Tru1I TTAA 1 cut(s) 367
Tru9I TTAA 1 cut(s) 367
TseFI GTSAC 3 cut(s) 52, 220, 257
Tsp45I GTSAC 3 cut(s) 52, 220, 257
TspGWI ACGGA 1 cut(s) 197
XapI RAATTY 2 cut(s) 20, 163
XhoI CTCGAG 1 cut(s) 86
XmiI GTMKAC 1 cut(s) 345
XspI CTAG 2 cut(s) 32, 168
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.