FvH4_2g28710

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0001875
Physical Location & Seq
Reverse (-)
22436363 .. 22436884
522 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g28710.t1

Sequence Viewer

Length: 369 bp
ATGGCAGATATGATAAAGTTTCTTTGCGTTGTTTTCATTTGCAGTCTCATTATTGGAGGTGATTGCCAATGCACTAACGCAGATCTTAATATCAAGCAGACATTTACCGGAAGGAAGTTCTATAATGGTGTTCCAGAGTTTAGCTTTAACCTCACTAATAGGTGCCGATGCGATGTACTGATTCAACTAGACTGCCGTGGGTTTAGACCTTGGATAACCATTGATCCTGCAGTATTGAACATATCCGGCGATAAGTGCATCCTCAACGGTGGCCAACCGATCCATCCGAATCAAGTTTTCAGCTTCAACTACGTTTTCCGCAATGAGTTCCCTTTCTTACCGATCACCTCTACGTACATTTGCTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

123

Amino Acids

13.85

Weight (kDa)

7.48

Isoelectric Point (pI)

32.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPD1_C PF24068 26 - 116 1.8e-18 Tapetum determinant 1, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 162
AciI CCGC 1 cut(s) 319
AclWI GGATC 2 cut(s) 218, 274
AcoI YGGCCR 1 cut(s) 271
AfaI GTAC 2 cut(s) 177, 356
AgsI TTSAA 3 cut(s) 185, 238, 307
AluBI AGCT 2 cut(s) 144, 303
AluI AGCT 2 cut(s) 144, 303
Alw26I GTCTC 1 cut(s) 50
AlwI GGATC 2 cut(s) 218, 274
AoxI GGCC 1 cut(s) 271
AsuHPI GGTGA 2 cut(s) 71, 337
BalI TGGCCA 1 cut(s) 273
BanI GGYRCC 1 cut(s) 162
BccI CCATC 1 cut(s) 291
BceAI ACGGC 1 cut(s) 180
BcoDI GTCTC 1 cut(s) 50
BfaI CTAG 1 cut(s) 188
BfmI CTRYAG 1 cut(s) 228
BglII AGATCT 1 cut(s) 82
BmiI GGNNCC 1 cut(s) 164
BmsI GCATC 2 cut(s) 158, 267
BsaAI YACGTR 1 cut(s) 354
BsaJI CCNNGG 2 cut(s) 196, 209
BsaWI WCCGGW 1 cut(s) 107
Bse3DI GCAATG 1 cut(s) 328
BseDI CCNNGG 2 cut(s) 196, 209
BseGI GGATG 2 cut(s) 258, 283
BseMI GCAATG 1 cut(s) 328
BshFI GGCC 1 cut(s) 273
BshNI GGYRCC 1 cut(s) 162
BsiSI CCGG 2 cut(s) 108, 246
BsmAI GTCTC 1 cut(s) 50
BsnI GGCC 1 cut(s) 273
Bsp143I GATC 4 cut(s) 82, 223, 279, 342
BspACI CCGC 1 cut(s) 319
BspANI GGCC 1 cut(s) 273
BspLI GGNNCC 1 cut(s) 164
BspMAI CTGCAG 1 cut(s) 232
BspPI GGATC 2 cut(s) 218, 274
BspT107I GGYRCC 1 cut(s) 162
BsrDI GCAATG 1 cut(s) 328
BssECI CCNNGG 2 cut(s) 196, 209
BssMI GATC 4 cut(s) 82, 223, 279, 342
BssT1I CCWWGG 1 cut(s) 209
Bst4CI ACNGT 1 cut(s) 269
BstBAI YACGTR 1 cut(s) 354
BstDSI CCRYGG 1 cut(s) 196
BstF5I GGATG 2 cut(s) 258, 283
BstKTI GATC 4 cut(s) 85, 226, 282, 345
BstMAI GTCTC 1 cut(s) 50
BstMBI GATC 4 cut(s) 82, 223, 279, 342
BstMWI GCNNNNNNNGC 1 cut(s) 255
BstSFI CTRYAG 1 cut(s) 228
BstSNI TACGTA 1 cut(s) 354
BstX2I RGATCY 1 cut(s) 82
BstYI RGATCY 1 cut(s) 82
BsuRI GGCC 1 cut(s) 273
BtgI CCRYGG 1 cut(s) 196
BtgZI GCGATG 1 cut(s) 186
BtsCI GGATG 2 cut(s) 258, 283
Csp6I GTAC 2 cut(s) 176, 355
CviJI RGCY 3 cut(s) 144, 273, 303
CviKI_1 RGCY 3 cut(s) 144, 273, 303
CviQI GTAC 2 cut(s) 176, 355
DpnI GATC 4 cut(s) 84, 225, 281, 344
DpnII GATC 4 cut(s) 82, 223, 279, 342
EaeI YGGCCR 1 cut(s) 271
Eco105I TACGTA 1 cut(s) 354
Eco130I CCWWGG 1 cut(s) 209
EcoT14I CCWWGG 1 cut(s) 209
ErhI CCWWGG 1 cut(s) 209
FaiI YATR 3 cut(s) 11, 123, 242
FokI GGATG 2 cut(s) 245, 270
FspBI CTAG 1 cut(s) 188
HaeIII GGCC 1 cut(s) 273
HapII CCGG 2 cut(s) 108, 246
HinfI GANTC 2 cut(s) 181, 289
HpaII CCGG 2 cut(s) 108, 246
HphI GGTGA 2 cut(s) 71, 337
Hpy188I TCNGA 1 cut(s) 288
Hpy188III TCNNGA 2 cut(s) 134, 366
HpyAV CCTTC 1 cut(s) 105
HpyCH4III ACNGT 1 cut(s) 269
HpyCH4IV ACGT 2 cut(s) 312, 353
HpyCH4V TGCA 4 cut(s) 42, 72, 230, 258
HpyF10VI GCNNNNNNNGC 1 cut(s) 255
HpySE526I ACGT 2 cut(s) 312, 353
Kzo9I GATC 4 cut(s) 82, 223, 279, 342
LpnPI CCDG 4 cut(s) 121, 147, 240, 259
LweI GCATC 2 cut(s) 158, 267
MaeI CTAG 1 cut(s) 188
MaeII ACGT 2 cut(s) 312, 353
MalI GATC 4 cut(s) 84, 225, 281, 344
MboI GATC 4 cut(s) 82, 223, 279, 342
MflI RGATCY 1 cut(s) 82
MlsI TGGCCA 1 cut(s) 273
MluNI TGGCCA 1 cut(s) 273
MnlI CCTC 4 cut(s) 50, 161, 272, 358
Mox20I TGGCCA 1 cut(s) 273
MscI TGGCCA 1 cut(s) 273
MseI TTAA 2 cut(s) 87, 147
Msp20I TGGCCA 1 cut(s) 273
MspI CCGG 2 cut(s) 108, 246
MwoI GCNNNNNNNGC 1 cut(s) 255
NdeII GATC 4 cut(s) 82, 223, 279, 342
NlaIV GGNNCC 1 cut(s) 164
PfeI GAWTC 2 cut(s) 181, 289
Ppu21I YACGTR 1 cut(s) 354
PspN4I GGNNCC 1 cut(s) 164
PstI CTGCAG 1 cut(s) 232
PsuI RGATCY 1 cut(s) 82
RsaI GTAC 2 cut(s) 177, 356
RsaNI GTAC 2 cut(s) 176, 355
SaqAI TTAA 2 cut(s) 87, 147
Sau3AI GATC 4 cut(s) 82, 223, 279, 342
SetI ASST 9 cut(s) 61, 146, 153, 164, 211, 305, 315, 350, 356
SfaNI GCATC 2 cut(s) 158, 267
SfcI CTRYAG 1 cut(s) 228
SgeI CNNG 9 cut(s) 106, 120, 146, 200, 209, 222, 239, 258, 305
SnaBI TACGTA 1 cut(s) 354
SsiI CCGC 1 cut(s) 319
SspMI CTAG 1 cut(s) 188
StyI CCWWGG 1 cut(s) 209
TaaI ACNGT 1 cut(s) 269
TaiI ACGT 2 cut(s) 315, 356
TatI WGTACW 1 cut(s) 175
TfiI GAWTC 2 cut(s) 181, 289
Tru1I TTAA 2 cut(s) 87, 147
Tru9I TTAA 2 cut(s) 87, 147
TspDTI ATGAA 1 cut(s) 25
XspI CTAG 1 cut(s) 188
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.