Rorug06G0272600

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0001875
Physical Location & Seq
Reverse (-)
42014948 .. 42018558
3611 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0272600.1

Sequence Viewer

Length: 936 bp
ATGAAAACCCTAGCAGCGCTCCCTCTCGCGCTAACTCTAGTGCCGTCTCCCCAGGTCGGCAATAAGCAGAGCCGCTCTGGCCGGCTTCCAACCCCAAGGCATTTGGGTCAGTTTCCTCCCATAACTTTTGAGCCTTCTAGTCTTCGGGTTCACCTACCCCTGTCGAGATCTGCTGCTGTTATCAATGACCATGGCGGCGGGATTGTTCTATTATGTCCAGACCGTGGAGACGCCTACGGCGGCCCAAACCAGCCTTCAAACCTCAGACCCTTGGGTTCGATCCTCTTTCTCTCGTCGACTGTCCTTGTTTTGTCAAGACTTGAACCGTCCTCTTGGGAAGGCTGCCTGCAATCGTCGTGGTGGCCTGATTGGTGGAGGCTAGATTTTCAGATCTCTGCACTGGATGGTGGCGGTGGCCTATACTATGGTAGAGAGGTTTTTACAATCAGGTTGCCTTCGTCAAGAAGCATAGATGCTCAATGGTGCAGTCCAAAGTCTGGGGATCGTCACCATGCTTCATCGTCGTCGGGTTGGTCTTGGCTGCTGGAGGTGTTGAAGTCAAGTTGTTTGATGGAGGCGCTGGAGGTGCGAAAAATTCTGCTTCGATTTGCAAGGGCAGTGACTAGAGGCGTCAGGCCTCGATCTGAGGGTCTGGAGACAGGCAATTTTGCATCCCGCCGAACTGGTGGTGGAACACCATGCATCGTGCCGGACAATGAACGCAAAAGCAGCTGGACTTGGAGAGAATTAGGGTTAGGGCTTTTCTTGAATCTTGAGAGCAATCGGAACCCGATGATCTACGACGTCAACTCCCCTCTTTTCAGGTCCTTCCTCAGCCAGAAGGGTGGCTCCTCCGATAAGAGGAAGATGGAAGAGCAGAAGCCAAAGGAACACAGGCCCAAGGCCAATGAGAACAAGCCCGTTATGAATGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

311

Amino Acids

34.54

Weight (kDa)

9.79

Isoelectric Point (pI)

60.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Wound_ind PF08186 265 - 311 1e-19 Wound-inducible basic protein family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 807
AccB7I CCANNNNNTGG 2 cut(s) 224, 497
AccBSI CCGCTC 1 cut(s) 75
AccI GTMKAC 1 cut(s) 296
AccII CGCG 1 cut(s) 29
AciI CCGC 6 cut(s) 73, 195, 198, 240, 411, 676
AclWI GGATC 2 cut(s) 274, 510
AcoI YGGCCR 1 cut(s) 79
AcsI RAATTY 1 cut(s) 594
AcyI GRCGYC 3 cut(s) 231, 630, 804
AfeI AGCGCT 1 cut(s) 18
AfiI CCNNNNNNNGG 4 cut(s) 56, 224, 497, 861
AgsI TTSAA 4 cut(s) 258, 323, 556, 769
AjnI CCWGG 1 cut(s) 51
AjuI GAANNNNNNNTTGG 2 cut(s) 238, 270
AluBI AGCT 1 cut(s) 732
AluI AGCT 1 cut(s) 732
Alw26I GTCTC 3 cut(s) 51, 222, 650
AlwI GGATC 2 cut(s) 274, 510
Aor51HI AGCGCT 1 cut(s) 18
AoxI GGCC 7 cut(s) 79, 241, 362, 415, 635, 896, 903
ApeKI GCWGC 5 cut(s) 14, 173, 342, 541, 729
ApoI RAATTY 1 cut(s) 594
AspLEI GCGC 3 cut(s) 19, 31, 580
AspS9I GGNCC 3 cut(s) 242, 825, 897
AsuHPI GGTGA 2 cut(s) 143, 500
AvaII GGWCC 1 cut(s) 825
BbsI GAAGAC 1 cut(s) 134
BbvCI CCTCAGC 1 cut(s) 833
BbvI GCAGC 5 cut(s) 26, 160, 329, 528, 741
BccI CCATC 3 cut(s) 398, 565, 862
BceAI ACGGC 2 cut(s) 28, 253
BciT130I CCWGG 1 cut(s) 53
BcoDI GTCTC 3 cut(s) 51, 222, 650
BfaI CTAG 5 cut(s) 11, 38, 138, 380, 624
BfoI RGCGCY 2 cut(s) 20, 581
BglI GCCNNNNNGGC 1 cut(s) 78
BglII AGATCT 2 cut(s) 167, 390
BisI GCNGC 8 cut(s) 15, 73, 174, 196, 241, 343, 542, 730
BlsI GCNGC 8 cut(s) 16, 74, 175, 197, 242, 344, 543, 731
Bme1390I CCNGG 1 cut(s) 53
Bme18I GGWCC 1 cut(s) 825
BmgT120I GGNCC 3 cut(s) 242, 825, 897
BmiI GGNNCC 2 cut(s) 788, 850
BmrFI CCNGG 1 cut(s) 53
BmsI GCATC 3 cut(s) 463, 680, 711
BpiI GAAGAC 1 cut(s) 134
BpmI CTGGAG 3 cut(s) 566, 602, 674
Bpu10I CCTNAGC 1 cut(s) 833
BpuEI CTTGAG 1 cut(s) 794
BsaHI GRCGYC 3 cut(s) 231, 630, 804
BsaJI CCNNGG 6 cut(s) 51, 95, 190, 223, 270, 900
BsaXI ACNNNNNCTCC 2 cut(s) 794, 824
Bsc4I CCNNNNNNNGG 4 cut(s) 56, 224, 497, 861
Bse118I RCCGGY 1 cut(s) 81
Bse1I ACTGG 2 cut(s) 405, 688
BseBI CCWGG 1 cut(s) 53
BseDI CCNNGG 6 cut(s) 51, 95, 190, 223, 270, 900
BseGI GGATG 2 cut(s) 409, 671
BseLI CCNNNNNNNGG 4 cut(s) 56, 224, 497, 861
BseMII CTCAG 3 cut(s) 277, 636, 847
BseNI ACTGG 2 cut(s) 405, 688
BseRI GAGGAG 1 cut(s) 841
BseXI GCAGC 5 cut(s) 26, 160, 329, 528, 741
BsgI GTGCAG 2 cut(s) 381, 505
Bsh1236I CGCG 1 cut(s) 29
BshFI GGCC 7 cut(s) 81, 243, 364, 417, 637, 898, 905
BsiSI CCGG 2 cut(s) 82, 710
BslI CCNNNNNNNGG 4 cut(s) 56, 224, 497, 861
BsmAI GTCTC 3 cut(s) 51, 222, 650
BsmBI CGTCTC 2 cut(s) 51, 222
BsnI GGCC 7 cut(s) 81, 243, 364, 417, 637, 898, 905
Bsp143I GATC 6 cut(s) 167, 279, 390, 502, 641, 795
Bsp19I CCATGG 1 cut(s) 190
BspACI CCGC 6 cut(s) 73, 195, 198, 240, 411, 676
BspANI GGCC 7 cut(s) 81, 243, 364, 417, 637, 898, 905
BspCNI CTCAG 3 cut(s) 276, 637, 846
BspFNI CGCG 1 cut(s) 29
BspLI GGNNCC 2 cut(s) 788, 850
BspPI GGATC 2 cut(s) 274, 510
BspQI GCTCTTC 1 cut(s) 867
BsrBI CCGCTC 1 cut(s) 75
BsrFI RCCGGY 1 cut(s) 81
BsrI ACTGG 2 cut(s) 405, 688
BssAI RCCGGY 1 cut(s) 81
BssECI CCNNGG 6 cut(s) 51, 95, 190, 223, 270, 900
BssMI GATC 6 cut(s) 167, 279, 390, 502, 641, 795
BssNI GRCGYC 3 cut(s) 231, 630, 804
BssT1I CCWWGG 4 cut(s) 95, 190, 270, 900
Bst2UI CCWGG 1 cut(s) 53
Bst4CI ACNGT 3 cut(s) 224, 301, 327
Bst6I CTCTTC 1 cut(s) 867
BstACI GRCGYC 3 cut(s) 231, 630, 804
BstC8I GCNNGC 2 cut(s) 83, 347
BstDEI CTNAG 3 cut(s) 263, 645, 833
BstDSI CCRYGG 2 cut(s) 190, 223
BstF5I GGATG 2 cut(s) 409, 671
BstFNI CGCG 1 cut(s) 29
BstH2I RGCGCY 2 cut(s) 20, 581
BstHHI GCGC 3 cut(s) 19, 31, 580
BstKTI GATC 6 cut(s) 170, 282, 393, 505, 644, 798
BstMAI GTCTC 3 cut(s) 51, 222, 650
BstMBI GATC 6 cut(s) 167, 279, 390, 502, 641, 795
BstMWI GCNNNNNNNGC 3 cut(s) 78, 586, 729
BstNI CCWGG 1 cut(s) 53
BstSCI CCNGG 1 cut(s) 51
BstUI CGCG 1 cut(s) 29
BstV1I GCAGC 5 cut(s) 26, 160, 329, 528, 741
BstV2I GAAGAC 1 cut(s) 134
BstX2I RGATCY 2 cut(s) 167, 390
BstXI CCANNNNNNTGG 1 cut(s) 845
BstYI RGATCY 2 cut(s) 167, 390
BsuRI GGCC 7 cut(s) 81, 243, 364, 417, 637, 898, 905
BtgI CCRYGG 2 cut(s) 190, 223
BtsCI GGATG 2 cut(s) 409, 671
BtsI GCAGTG 1 cut(s) 624
BtsIMutI CAGTG 2 cut(s) 398, 624
Cac8I GCNNGC 2 cut(s) 83, 347
CfoI GCGC 3 cut(s) 19, 31, 580
Cfr10I RCCGGY 1 cut(s) 81
Cfr13I GGNCC 3 cut(s) 242, 825, 897
CseI GACGC 2 cut(s) 239, 619
CspCI CAANNNNNGTGG 2 cut(s) 338, 373
CviAII CATG 3 cut(s) 191, 512, 699
DdeI CTNAG 3 cut(s) 263, 645, 833
DpnI GATC 6 cut(s) 169, 281, 392, 504, 643, 797
DpnII GATC 6 cut(s) 167, 279, 390, 502, 641, 795
EaeI YGGCCR 1 cut(s) 79
Eam1104I CTCTTC 1 cut(s) 867
EarI CTCTTC 1 cut(s) 867
Eco130I CCWWGG 4 cut(s) 95, 190, 270, 900
Eco147I AGGCCT 1 cut(s) 637
Eco47I GGWCC 1 cut(s) 825
Eco47III AGCGCT 1 cut(s) 18
EcoO109I RGGNCCY 1 cut(s) 825
EcoRII CCWGG 1 cut(s) 51
EcoT14I CCWWGG 4 cut(s) 95, 190, 270, 900
EcoT22I ATGCAT 1 cut(s) 704
ErhI CCWWGG 4 cut(s) 95, 190, 270, 900
Esp3I CGTCTC 2 cut(s) 51, 222
FaeI CATG 3 cut(s) 194, 515, 702
FaiI YATR 9 cut(s) 122, 192, 214, 421, 426, 470, 513, 700, 926
FatI CATG 3 cut(s) 190, 511, 698
FauI CCCGC 2 cut(s) 191, 683
FblI GTMKAC 1 cut(s) 296
Fnu4HI GCNGC 8 cut(s) 15, 73, 174, 196, 241, 343, 542, 730
FokI GGATG 2 cut(s) 416, 658
Fsp4HI GCNGC 8 cut(s) 15, 73, 174, 196, 241, 343, 542, 730
FspBI CTAG 5 cut(s) 11, 38, 138, 380, 624
GlaI GCGC 3 cut(s) 18, 30, 579
GluI GCNGC 8 cut(s) 15, 73, 174, 196, 241, 343, 542, 730
GsuI CTGGAG 3 cut(s) 566, 602, 674
HaeII RGCGCY 2 cut(s) 20, 581
HaeIII GGCC 7 cut(s) 81, 243, 364, 417, 637, 898, 905
HapII CCGG 2 cut(s) 82, 710
HgaI GACGC 2 cut(s) 239, 619
HhaI GCGC 3 cut(s) 19, 31, 580
Hin1I GRCGYC 3 cut(s) 231, 630, 804
Hin1II CATG 3 cut(s) 194, 515, 702
Hin6I GCGC 3 cut(s) 17, 29, 578
HinP1I GCGC 3 cut(s) 17, 29, 578
HincII GTYRAC 2 cut(s) 297, 808
HindII GTYRAC 2 cut(s) 297, 808
HinfI GANTC 1 cut(s) 769
HpaII CCGG 2 cut(s) 82, 710
HphI GGTGA 2 cut(s) 143, 500
Hpy166II GTNNAC 3 cut(s) 151, 297, 808
Hpy188I TCNGA 5 cut(s) 266, 390, 646, 786, 856
Hpy188III TCNNGA 7 cut(s) 165, 218, 315, 462, 653, 766, 773
Hpy8I GTNNAC 3 cut(s) 151, 297, 808
Hpy99I CGWCG 5 cut(s) 298, 358, 526, 529, 806
HpyAV CCTTC 6 cut(s) 144, 264, 332, 465, 835, 838
HpyCH4III ACNGT 3 cut(s) 224, 301, 327
HpyCH4IV ACGT 1 cut(s) 804
HpyCH4V TGCA 6 cut(s) 349, 398, 486, 611, 671, 702
HpyF10VI GCNNNNNNNGC 3 cut(s) 78, 586, 729
HpyF3I CTNAG 3 cut(s) 263, 645, 833
HpySE526I ACGT 1 cut(s) 804
Hsp92I GRCGYC 3 cut(s) 231, 630, 804
Hsp92II CATG 3 cut(s) 194, 515, 702
HspAI GCGC 3 cut(s) 17, 29, 578
KroI GCCGGC 1 cut(s) 81
KroNI GCCGGC 1 cut(s) 83
Kzo9I GATC 6 cut(s) 167, 279, 390, 502, 641, 795
LguI GCTCTTC 1 cut(s) 867
LmnI GCTCC 2 cut(s) 24, 854
Lsp1109I GCAGC 5 cut(s) 26, 160, 329, 528, 741
LweI GCATC 3 cut(s) 463, 680, 711
MaeI CTAG 5 cut(s) 11, 38, 138, 380, 624
MaeII ACGT 1 cut(s) 804
MaeIII GTNAC 2 cut(s) 506, 619
MalI GATC 6 cut(s) 169, 281, 392, 504, 643, 797
MbiI CCGCTC 1 cut(s) 75
MboI GATC 6 cut(s) 167, 279, 390, 502, 641, 795
MboII GAAGA 3 cut(s) 134, 877, 884
MflI RGATCY 2 cut(s) 167, 390
MluCI AATT 3 cut(s) 594, 664, 746
MmeI TCCRAC 1 cut(s) 113
Mph1103I ATGCAT 1 cut(s) 704
MroNI GCCGGC 1 cut(s) 81
MspA1I CMGCKG 1 cut(s) 732
MspI CCGG 2 cut(s) 82, 710
MspR9I CCNGG 1 cut(s) 53
MvaI CCWGG 1 cut(s) 53
MvnI CGCG 1 cut(s) 29
MwoI GCNNNNNNNGC 3 cut(s) 78, 586, 729
NaeI GCCGGC 1 cut(s) 83
NcoI CCATGG 1 cut(s) 190
NdeII GATC 6 cut(s) 167, 279, 390, 502, 641, 795
NgoMIV GCCGGC 1 cut(s) 81
NlaIII CATG 3 cut(s) 194, 515, 702
NlaIV GGNNCC 2 cut(s) 788, 850
NmuCI GTSAC 2 cut(s) 506, 619
NsiI ATGCAT 1 cut(s) 704
PceI AGGCCT 1 cut(s) 637
PciSI GCTCTTC 1 cut(s) 867
PdiI GCCGGC 1 cut(s) 83
PfeI GAWTC 1 cut(s) 769
PflMI CCANNNNNTGG 2 cut(s) 224, 497
PkrI GCNGC 8 cut(s) 16, 74, 175, 197, 242, 344, 543, 731
PpuMI RGGWCCY 1 cut(s) 825
Psp5II RGGWCCY 1 cut(s) 825
Psp6I CCWGG 1 cut(s) 51
PspGI CCWGG 1 cut(s) 51
PspN4I GGNNCC 2 cut(s) 788, 850
PspPI GGNCC 3 cut(s) 242, 825, 897
PspPPI RGGWCCY 1 cut(s) 825
PsuI RGATCY 2 cut(s) 167, 390
PvuII CAGCTG 1 cut(s) 732
SalI GTCGAC 1 cut(s) 295
SapI GCTCTTC 1 cut(s) 867
SatI GCNGC 8 cut(s) 15, 73, 174, 196, 241, 343, 542, 730
Sau3AI GATC 6 cut(s) 167, 279, 390, 502, 641, 795
Sau96I GGNCC 3 cut(s) 242, 825, 897
ScrFI CCNGG 1 cut(s) 53
SfaNI GCATC 3 cut(s) 463, 680, 711
SinI GGWCC 1 cut(s) 825
SmlI CTYRAG 1 cut(s) 773
SmoI CTYRAG 1 cut(s) 773
Sse9I AATT 3 cut(s) 594, 664, 746
SseBI AGGCCT 1 cut(s) 637
SsiI CCGC 6 cut(s) 73, 195, 198, 240, 411, 676
SspMI CTAG 5 cut(s) 11, 38, 138, 380, 624
StuI AGGCCT 1 cut(s) 637
StyD4I CCNGG 1 cut(s) 51
StyI CCWWGG 4 cut(s) 95, 190, 270, 900
TaaI ACNGT 3 cut(s) 224, 301, 327
TaiI ACGT 1 cut(s) 807
TaqI TCGA 5 cut(s) 164, 278, 296, 604, 640
TasI AATT 3 cut(s) 594, 664, 746
TauI GCSGC 3 cut(s) 75, 198, 243
TfiI GAWTC 1 cut(s) 769
TscAI CASTG 2 cut(s) 405, 624
TseFI GTSAC 2 cut(s) 506, 619
TseI GCWGC 5 cut(s) 14, 173, 342, 541, 729
Tsp45I GTSAC 2 cut(s) 506, 619
TspDTI ATGAA 3 cut(s) 17, 507, 732
TspRI CASTG 2 cut(s) 405, 624
Van91I CCANNNNNTGG 2 cut(s) 224, 497
VpaK11BI GGWCC 1 cut(s) 825
XapI RAATTY 1 cut(s) 594
XmiI GTMKAC 1 cut(s) 296
XspI CTAG 5 cut(s) 11, 38, 138, 380, 624
ZraI GACGTC 1 cut(s) 805
Zsp2I ATGCAT 1 cut(s) 704
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.