MD15G1128800.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0001875
Physical Location & Seq
Reverse (-)
9319221 .. 9320039
819 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1128800.v1.1.491

Sequence Viewer

Length: 483 bp
ATGCCTTACTATAGTTGTCGGTTACTGTATATAAATCAGACCATGAACGCAGCCACCATCCATCTTCCCCAAAGAATTTCAGTTCCGAAACGCACAAATTTCTCCGAGATGGCTGCCTTGGTCAAGTCCTTCATTGTGATTCTTCTTTTCAGTCTCATTAACGAAGGAATATGCCAAGAAAAGTGCGCTACGATCGACCTGCAAATTAGACAAGAGAAAACAGGGAATCTGGTGCAAGCAAAGCCAGAGTTTCAGGTGAGGGTACTGAATGCATGCCCATGTCTGCAAGGGAATGTGACATTAGACTGCAATGGATTTCAAACCGTTGAAGTCGAAAACCCCGTGACATTGCTCAGATCTGGGAATGAGTGTCTCCTCAACAATGGCTCATTCCTTACACCATTCAATGAAGTTGTCTTCGTCTATGCTTGGGACACACAGTTTCCATTCAAGCCACTTTCCTCTAACATTAAATGTGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

161

Amino Acids

18.06

Weight (kDa)

6.56

Isoelectric Point (pI)

43.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPD1_C PF24068 66 - 156 1.3e-25 Tapetum determinant 1, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 207
AcsI RAATTY 2 cut(s) 75, 97
AfaI GTAC 1 cut(s) 264
AgsI TTSAA 4 cut(s) 320, 329, 406, 451
Alw26I GTCTC 2 cut(s) 158, 377
ApeKI GCWGC 2 cut(s) 50, 113
ApoI RAATTY 2 cut(s) 75, 97
AspLEI GCGC 1 cut(s) 188
AsuHPI GGTGA 1 cut(s) 268
BbsI GAAGAC 1 cut(s) 409
BbvI GCAGC 2 cut(s) 62, 100
BccI CCATC 3 cut(s) 65, 69, 103
BcgI CGANNNNNNTGC 4 cut(s) 95, 129, 181, 215
BcoDI GTCTC 2 cut(s) 158, 377
BfmI CTRYAG 1 cut(s) 10
BfuAI ACCTGC 1 cut(s) 207
BglII AGATCT 1 cut(s) 356
BisI GCNGC 2 cut(s) 51, 114
BlsI GCNGC 2 cut(s) 52, 115
BpiI GAAGAC 1 cut(s) 409
BsaJI CCNNGG 1 cut(s) 117
Bse3DI GCAATG 2 cut(s) 316, 347
BseDI CCNNGG 1 cut(s) 117
BseGI GGATG 1 cut(s) 57
BseMI GCAATG 2 cut(s) 316, 347
BseMII CTCAG 1 cut(s) 367
BseRI GAGGAG 1 cut(s) 365
BseXI GCAGC 2 cut(s) 62, 100
Bsh1285I CGRYCG 1 cut(s) 195
BsiEI CGRYCG 1 cut(s) 195
BslFI GGGAC 1 cut(s) 446
BsmAI GTCTC 2 cut(s) 158, 377
BsmFI GGGAC 1 cut(s) 446
BsmI GAATGC 1 cut(s) 274
Bsp143I GATC 2 cut(s) 192, 356
BspCNI CTCAG 1 cut(s) 366
BspMI ACCTGC 1 cut(s) 207
BsrDI GCAATG 2 cut(s) 316, 347
BssECI CCNNGG 1 cut(s) 117
BssMI GATC 2 cut(s) 192, 356
BssT1I CCWWGG 1 cut(s) 117
Bst4CI ACNGT 3 cut(s) 27, 325, 441
BstC8I GCNNGC 2 cut(s) 237, 274
BstDEI CTNAG 1 cut(s) 353
BstF5I GGATG 1 cut(s) 57
BstHHI GCGC 1 cut(s) 188
BstKTI GATC 2 cut(s) 195, 359
BstMAI GTCTC 2 cut(s) 158, 377
BstMBI GATC 2 cut(s) 192, 356
BstMCI CGRYCG 1 cut(s) 195
BstMWI GCNNNNNNNGC 1 cut(s) 241
BstNSI RCATGY 1 cut(s) 276
BstSFI CTRYAG 1 cut(s) 10
BstV1I GCAGC 2 cut(s) 62, 100
BstV2I GAAGAC 1 cut(s) 409
BstX2I RGATCY 1 cut(s) 356
BstYI RGATCY 1 cut(s) 356
BtsCI GGATG 1 cut(s) 57
BveI ACCTGC 1 cut(s) 207
Cac8I GCNNGC 2 cut(s) 237, 274
CfoI GCGC 1 cut(s) 188
Csp6I GTAC 1 cut(s) 263
CviAII CATG 3 cut(s) 43, 273, 279
CviJI RGCY 5 cut(s) 53, 113, 244, 387, 454
CviKI_1 RGCY 5 cut(s) 53, 113, 244, 387, 454
CviQI GTAC 1 cut(s) 263
DdeI CTNAG 1 cut(s) 353
DpnI GATC 2 cut(s) 194, 358
DpnII GATC 2 cut(s) 192, 356
Eco130I CCWWGG 1 cut(s) 117
EcoT14I CCWWGG 1 cut(s) 117
EcoT22I ATGCAT 1 cut(s) 274
ErhI CCWWGG 1 cut(s) 117
FaeI CATG 3 cut(s) 46, 276, 282
FaiI YATR 8 cut(s) 12, 30, 32, 44, 172, 274, 280, 426
FaqI GGGAC 1 cut(s) 446
FatI CATG 3 cut(s) 42, 272, 278
Fnu4HI GCNGC 2 cut(s) 51, 114
FokI GGATG 1 cut(s) 44
Fsp4HI GCNGC 2 cut(s) 51, 114
GlaI GCGC 1 cut(s) 187
GluI GCNGC 2 cut(s) 51, 114
HhaI GCGC 1 cut(s) 188
Hin1II CATG 3 cut(s) 46, 276, 282
Hin6I GCGC 1 cut(s) 186
HinP1I GCGC 1 cut(s) 186
HinfI GANTC 2 cut(s) 139, 226
HphI GGTGA 1 cut(s) 268
Hpy188I TCNGA 4 cut(s) 39, 87, 106, 356
HpyAV CCTTC 2 cut(s) 139, 158
HpyCH4III ACNGT 3 cut(s) 27, 325, 441
HpyCH4V TGCA 5 cut(s) 202, 235, 272, 286, 309
HpyF10VI GCNNNNNNNGC 1 cut(s) 241
HpyF3I CTNAG 1 cut(s) 353
Hsp92II CATG 3 cut(s) 46, 276, 282
HspAI GCGC 1 cut(s) 186
Kzo9I GATC 2 cut(s) 192, 356
LpnPI CCDG 6 cut(s) 207, 212, 215, 239, 258, 345
Lsp1109I GCAGC 2 cut(s) 62, 100
MaeIII GTNAC 3 cut(s) 21, 295, 343
MalI GATC 2 cut(s) 194, 358
MboI GATC 2 cut(s) 192, 356
MboII GAAGA 3 cut(s) 56, 134, 409
MflI RGATCY 1 cut(s) 356
MluCI AATT 3 cut(s) 75, 97, 204
MnlI CCTC 3 cut(s) 252, 386, 472
Mph1103I ATGCAT 1 cut(s) 274
MseI TTAA 2 cut(s) 159, 471
MslI CAYNNNNRTG 1 cut(s) 277
Mva1269I GAATGC 1 cut(s) 274
MwoI GCNNNNNNNGC 1 cut(s) 241
NdeII GATC 2 cut(s) 192, 356
NlaIII CATG 3 cut(s) 46, 276, 282
NmuCI GTSAC 2 cut(s) 295, 343
NsiI ATGCAT 1 cut(s) 274
NspI RCATGY 1 cut(s) 276
PaeI GCATGC 1 cut(s) 276
PcsI WCGNNNNNNNCGW 1 cut(s) 339
PctI GAATGC 1 cut(s) 274
PfeI GAWTC 2 cut(s) 139, 226
PkrI GCNGC 2 cut(s) 52, 115
Ple19I CGATCG 1 cut(s) 195
PsuI RGATCY 1 cut(s) 356
PvuI CGATCG 1 cut(s) 195
RsaI GTAC 1 cut(s) 264
RsaNI GTAC 1 cut(s) 263
RseI CAYNNNNRTG 1 cut(s) 277
SaqAI TTAA 2 cut(s) 159, 471
SatI GCNGC 2 cut(s) 51, 114
Sau3AI GATC 2 cut(s) 192, 356
SetI ASST 2 cut(s) 201, 258
SfcI CTRYAG 1 cut(s) 10
SmiMI CAYNNNNRTG 1 cut(s) 277
SphI GCATGC 1 cut(s) 276
Sse9I AATT 3 cut(s) 75, 97, 204
StyI CCWWGG 1 cut(s) 117
TaaI ACNGT 3 cut(s) 27, 325, 441
TaqI TCGA 2 cut(s) 195, 333
TasI AATT 3 cut(s) 75, 97, 204
TfiI GAWTC 2 cut(s) 139, 226
Tru1I TTAA 2 cut(s) 159, 471
Tru9I TTAA 2 cut(s) 159, 471
TseFI GTSAC 2 cut(s) 295, 343
TseI GCWGC 2 cut(s) 50, 113
Tsp45I GTSAC 2 cut(s) 295, 343
TspDTI ATGAA 3 cut(s) 59, 121, 423
XapI RAATTY 2 cut(s) 75, 97
XceI RCATGY 1 cut(s) 276
Zsp2I ATGCAT 1 cut(s) 274
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.