Rw6G033410

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0001875
Physical Location & Seq
Reverse (-)
57958065 .. 57958505
441 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G033410.1

Sequence Viewer

Length: 363 bp
ATGAAGCAGTTTCTCCGCGCAGTTTTCCTCTTCGGTCTCAATGTTGGAGGCCAGTGCTTTGATTGCTCTGTGAAGGATATCTCAATTGAACAATCTCAGACGGGAAAGTCGGTCCAAAACAAGCCAGAATGGAATGTGACGATCGCGAATGGTTGCTCGTGTTCTCAACTGAATGTTAAACTAGGCTGCGATGGATTTCAAACTGTTGAAGACATTGATCCTTCCATTTTAAGTGTATCTGGTGGTGAGTGTCTGGTCAAGAATGGCCAACCTGTCTATGCCAATGGAGGTTTCAACTTCATCTATGCTTGGGACACTTCATTTTCTTTCAATCCAATCTCCTCCCAAATTGCTTGCTCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

120

Amino Acids

12.95

Weight (kDa)

4.61

Isoelectric Point (pI)

47.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPD1_C PF24068 24 - 116 2.1e-30 Tapetum determinant 1, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 106
AccII CGCG 2 cut(s) 18, 146
AciI CCGC 1 cut(s) 16
AclWI GGATC 1 cut(s) 212
AcoI YGGCCR 1 cut(s) 265
AgsI TTSAA 5 cut(s) 89, 200, 209, 295, 331
AjuI GAANNNNNNNTTGG 2 cut(s) 275, 307
Alw26I GTCTC 1 cut(s) 41
AlwI GGATC 1 cut(s) 212
AoxI GGCC 2 cut(s) 49, 265
ApeKI GCWGC 1 cut(s) 186
AspLEI GCGC 1 cut(s) 20
AspS9I GGNCC 1 cut(s) 112
AsuHPI GGTGA 1 cut(s) 257
AvaII GGWCC 1 cut(s) 112
BalI TGGCCA 1 cut(s) 267
BauI CACGAG 1 cut(s) 157
BbsI GAAGAC 1 cut(s) 216
BbvI GCAGC 1 cut(s) 173
BccI CCATC 1 cut(s) 185
BcoDI GTCTC 1 cut(s) 41
BfaI CTAG 1 cut(s) 182
BisI GCNGC 1 cut(s) 187
BlsI GCNGC 1 cut(s) 188
Bme18I GGWCC 1 cut(s) 112
BmgT120I GGNCC 1 cut(s) 112
BpiI GAAGAC 1 cut(s) 216
BsaI GGTCTC 1 cut(s) 41
Bse1I ACTGG 1 cut(s) 52
BseMII CTCAG 1 cut(s) 110
BseNI ACTGG 1 cut(s) 52
BseRI GAGGAG 1 cut(s) 331
BseXI GCAGC 1 cut(s) 173
Bsh1236I CGCG 2 cut(s) 18, 146
Bsh1285I CGRYCG 1 cut(s) 144
BshFI GGCC 2 cut(s) 51, 267
BsiEI CGRYCG 1 cut(s) 144
BslFI GGGAC 1 cut(s) 326
BsmAI GTCTC 1 cut(s) 41
BsmFI GGGAC 1 cut(s) 326
BsnI GGCC 2 cut(s) 51, 267
Bso31I GGTCTC 1 cut(s) 41
Bsp143I GATC 2 cut(s) 141, 217
Bsp68I TCGCGA 1 cut(s) 146
BspACI CCGC 1 cut(s) 16
BspANI GGCC 2 cut(s) 51, 267
BspCNI CTCAG 1 cut(s) 109
BspFNI CGCG 2 cut(s) 18, 146
BspPI GGATC 1 cut(s) 212
BspTNI GGTCTC 1 cut(s) 41
BsrI ACTGG 1 cut(s) 52
BssMI GATC 2 cut(s) 141, 217
BssSI CACGAG 1 cut(s) 157
Bst2BI CACGAG 1 cut(s) 157
Bst4CI ACNGT 1 cut(s) 205
Bst6I CTCTTC 1 cut(s) 35
BstC8I GCNNGC 1 cut(s) 355
BstDEI CTNAG 1 cut(s) 96
BstFNI CGCG 2 cut(s) 18, 146
BstHHI GCGC 1 cut(s) 20
BstKTI GATC 2 cut(s) 144, 220
BstMAI GTCTC 1 cut(s) 41
BstMBI GATC 2 cut(s) 141, 217
BstMCI CGRYCG 1 cut(s) 144
BstMWI GCNNNNNNNGC 1 cut(s) 63
BstUI CGCG 2 cut(s) 18, 146
BstV1I GCAGC 1 cut(s) 173
BstV2I GAAGAC 1 cut(s) 216
BsuRI GGCC 2 cut(s) 51, 267
BtgZI GCGATG 1 cut(s) 204
BtsIMutI CAGTG 1 cut(s) 59
BtuMI TCGCGA 1 cut(s) 146
Cac8I GCNNGC 1 cut(s) 355
CfoI GCGC 1 cut(s) 20
Cfr13I GGNCC 1 cut(s) 112
CviJI RGCY 4 cut(s) 51, 124, 186, 267
CviKI_1 RGCY 4 cut(s) 51, 124, 186, 267
DdeI CTNAG 1 cut(s) 96
DpnI GATC 2 cut(s) 143, 219
DpnII GATC 2 cut(s) 141, 217
DrdI GACNNNNNNGTC 1 cut(s) 106
DseDI GACNNNNNNGTC 1 cut(s) 106
EaeI YGGCCR 1 cut(s) 265
Eam1104I CTCTTC 1 cut(s) 35
EarI CTCTTC 1 cut(s) 35
Eco31I GGTCTC 1 cut(s) 41
Eco32I GATATC 1 cut(s) 79
Eco47I GGWCC 1 cut(s) 112
EcoRV GATATC 1 cut(s) 79
FaiI YATR 2 cut(s) 279, 306
FaqI GGGAC 1 cut(s) 326
Fnu4HI GCNGC 1 cut(s) 187
Fsp4HI GCNGC 1 cut(s) 187
FspBI CTAG 1 cut(s) 182
GlaI GCGC 1 cut(s) 19
GluI GCNGC 1 cut(s) 187
HaeIII GGCC 2 cut(s) 51, 267
HhaI GCGC 1 cut(s) 20
Hin6I GCGC 1 cut(s) 18
HinP1I GCGC 1 cut(s) 18
HphI GGTGA 1 cut(s) 257
Hpy188I TCNGA 1 cut(s) 99
Hpy188III TCNNGA 3 cut(s) 145, 259, 360
HpyAV CCTTC 2 cut(s) 67, 231
HpyCH4III ACNGT 1 cut(s) 205
HpyF10VI GCNNNNNNNGC 1 cut(s) 63
HpyF3I CTNAG 1 cut(s) 96
HspAI GCGC 1 cut(s) 18
Kzo9I GATC 2 cut(s) 141, 217
LmnI GCTCC 1 cut(s) 362
LpnPI CCDG 5 cut(s) 65, 138, 225, 239, 285
Lsp1109I GCAGC 1 cut(s) 173
MaeI CTAG 1 cut(s) 182
MaeIII GTNAC 1 cut(s) 136
MalI GATC 2 cut(s) 143, 219
MboI GATC 2 cut(s) 141, 217
MboII GAAGA 2 cut(s) 22, 221
MfeI CAATTG 1 cut(s) 84
MlsI TGGCCA 1 cut(s) 267
MluCI AATT 2 cut(s) 84, 348
MluNI TGGCCA 1 cut(s) 267
MmeI TCCRAC 1 cut(s) 25
MnlI CCTC 4 cut(s) 38, 41, 281, 352
Mox20I TGGCCA 1 cut(s) 267
MscI TGGCCA 1 cut(s) 267
MseI TTAA 2 cut(s) 177, 230
Msp20I TGGCCA 1 cut(s) 267
MunI CAATTG 1 cut(s) 84
MvnI CGCG 2 cut(s) 18, 146
MwoI GCNNNNNNNGC 1 cut(s) 63
NdeII GATC 2 cut(s) 141, 217
NmuCI GTSAC 1 cut(s) 136
NruI TCGCGA 1 cut(s) 146
PkrI GCNGC 1 cut(s) 188
Ple19I CGATCG 1 cut(s) 144
PspPI GGNCC 1 cut(s) 112
PvuI CGATCG 1 cut(s) 144
RruI TCGCGA 1 cut(s) 146
SaqAI TTAA 2 cut(s) 177, 230
SatI GCNGC 1 cut(s) 187
Sau3AI GATC 2 cut(s) 141, 217
Sau96I GGNCC 1 cut(s) 112
SetI ASST 2 cut(s) 274, 292
SinI GGWCC 1 cut(s) 112
Sse9I AATT 2 cut(s) 84, 348
SsiI CCGC 1 cut(s) 16
SspMI CTAG 1 cut(s) 182
TaaI ACNGT 1 cut(s) 205
TaqII GACCGA 2 cut(s) 23, 100
TasI AATT 2 cut(s) 84, 348
Tru1I TTAA 2 cut(s) 177, 230
Tru9I TTAA 2 cut(s) 177, 230
TscAI CASTG 1 cut(s) 59
TseFI GTSAC 1 cut(s) 136
TseI GCWGC 1 cut(s) 186
Tsp45I GTSAC 1 cut(s) 136
TspDTI ATGAA 3 cut(s) 17, 289, 309
TspRI CASTG 1 cut(s) 59
VpaK11BI GGWCC 1 cut(s) 112
XspI CTAG 1 cut(s) 182
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.