RchiOBHm_Chr6g0297461

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0001875
Physical Location & Seq
Reverse (-)
59198620 .. 59200492
1873 bp
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UTR
Exon/CDS
Intron
PRQ26698

Sequence Viewer

Length: 405 bp
ATGAAGTTCTGTGTTCAAATGCAGCAGCAGCAGCTATACATCATGAAGCAGTTTCTCTGCGCAGTTTTCCTCTTCGGTCTCAATGTTGGAGGCCAGTGCTTTGGTTGCTCTGTGAAGGATATCTCAATTGAACAATCTCAGACGGGAAAGTCGGTCAAAAACAAGCCAGAATGGAATGTGACGATCGCGAATGGTTGCTCGTGTTCTCAACTGAATGTTAAACTAGCCTGCGATGGATTTCAAACTGTTGAAGACATTGATCCTTCCATTTTAAGTGTATCTGGTGGTGAGTGTCTAGTCAAGAATGGCCAACCTGTCTATGCCAATGGAGGTTTCAACTTCTTCTATGCTTGGGACACTTCATTTTCTTTCAATCCAATCTCCTCCCAAATTGCTTGCTCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

134

Amino Acids

14.65

Weight (kDa)

5.17

Isoelectric Point (pI)

49.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPD1_C PF24068 38 - 130 1.7e-30 Tapetum determinant 1, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 148
Acc16I TGCGCA 1 cut(s) 61
AccII CGCG 1 cut(s) 188
AclWI GGATC 1 cut(s) 254
AcoI YGGCCR 1 cut(s) 307
AgsI TTSAA 6 cut(s) 17, 131, 242, 251, 337, 373
AjuI GAANNNNNNNTTGG 2 cut(s) 317, 349
AluBI AGCT 1 cut(s) 34
AluI AGCT 1 cut(s) 34
Alw26I GTCTC 1 cut(s) 83
AlwI GGATC 1 cut(s) 254
AoxI GGCC 2 cut(s) 91, 307
ApeKI GCWGC 4 cut(s) 22, 25, 28, 31
AspLEI GCGC 1 cut(s) 62
AsuHPI GGTGA 1 cut(s) 299
BalI TGGCCA 1 cut(s) 309
BauI CACGAG 1 cut(s) 199
BbsI GAAGAC 1 cut(s) 258
BbvI GCAGC 4 cut(s) 34, 37, 40, 43
BccI CCATC 1 cut(s) 227
BcoDI GTCTC 1 cut(s) 83
BfaI CTAG 2 cut(s) 224, 296
BisI GCNGC 4 cut(s) 23, 26, 29, 32
BlsI GCNGC 4 cut(s) 24, 27, 30, 33
BpiI GAAGAC 1 cut(s) 258
BsaI GGTCTC 1 cut(s) 83
Bse1I ACTGG 1 cut(s) 94
BseMII CTCAG 1 cut(s) 152
BseNI ACTGG 1 cut(s) 94
BseRI GAGGAG 1 cut(s) 373
BseXI GCAGC 4 cut(s) 34, 37, 40, 43
Bsh1236I CGCG 1 cut(s) 188
Bsh1285I CGRYCG 1 cut(s) 186
BshFI GGCC 2 cut(s) 93, 309
BsiEI CGRYCG 1 cut(s) 186
BslFI GGGAC 1 cut(s) 368
BsmAI GTCTC 1 cut(s) 83
BsmFI GGGAC 1 cut(s) 368
BsnI GGCC 2 cut(s) 93, 309
Bso31I GGTCTC 1 cut(s) 83
Bsp143I GATC 2 cut(s) 183, 259
Bsp68I TCGCGA 1 cut(s) 188
BspANI GGCC 2 cut(s) 93, 309
BspCNI CTCAG 1 cut(s) 151
BspFNI CGCG 1 cut(s) 188
BspHI TCATGA 1 cut(s) 42
BspPI GGATC 1 cut(s) 254
BspTNI GGTCTC 1 cut(s) 83
BsrI ACTGG 1 cut(s) 94
BssMI GATC 2 cut(s) 183, 259
BssSI CACGAG 1 cut(s) 199
Bst2BI CACGAG 1 cut(s) 199
Bst4CI ACNGT 1 cut(s) 247
Bst6I CTCTTC 1 cut(s) 77
BstC8I GCNNGC 2 cut(s) 229, 397
BstDEI CTNAG 1 cut(s) 138
BstFNI CGCG 1 cut(s) 188
BstHHI GCGC 1 cut(s) 62
BstKTI GATC 2 cut(s) 186, 262
BstMAI GTCTC 1 cut(s) 83
BstMBI GATC 2 cut(s) 183, 259
BstMCI CGRYCG 1 cut(s) 186
BstMWI GCNNNNNNNGC 3 cut(s) 28, 31, 105
BstUI CGCG 1 cut(s) 188
BstV1I GCAGC 4 cut(s) 34, 37, 40, 43
BstV2I GAAGAC 1 cut(s) 258
BstXI CCANNNNNNTGG 1 cut(s) 101
BsuRI GGCC 2 cut(s) 93, 309
BtgZI GCGATG 1 cut(s) 246
BtsIMutI CAGTG 1 cut(s) 101
BtuMI TCGCGA 1 cut(s) 188
Cac8I GCNNGC 2 cut(s) 229, 397
CciI TCATGA 1 cut(s) 42
CfoI GCGC 1 cut(s) 62
CviAII CATG 1 cut(s) 43
CviJI RGCY 5 cut(s) 34, 93, 166, 227, 309
CviKI_1 RGCY 5 cut(s) 34, 93, 166, 227, 309
DdeI CTNAG 1 cut(s) 138
DpnI GATC 2 cut(s) 185, 261
DpnII GATC 2 cut(s) 183, 259
DrdI GACNNNNNNGTC 1 cut(s) 148
DseDI GACNNNNNNGTC 1 cut(s) 148
EaeI YGGCCR 1 cut(s) 307
Eam1104I CTCTTC 1 cut(s) 77
EarI CTCTTC 1 cut(s) 77
Eco31I GGTCTC 1 cut(s) 83
Eco32I GATATC 1 cut(s) 121
EcoRV GATATC 1 cut(s) 121
FaeI CATG 1 cut(s) 46
FaiI YATR 4 cut(s) 37, 44, 321, 348
FaqI GGGAC 1 cut(s) 368
FatI CATG 1 cut(s) 42
Fnu4HI GCNGC 4 cut(s) 23, 26, 29, 32
Fsp4HI GCNGC 4 cut(s) 23, 26, 29, 32
FspBI CTAG 2 cut(s) 224, 296
FspI TGCGCA 1 cut(s) 61
GlaI GCGC 1 cut(s) 61
GluI GCNGC 4 cut(s) 23, 26, 29, 32
HaeIII GGCC 2 cut(s) 93, 309
HhaI GCGC 1 cut(s) 62
Hin1II CATG 1 cut(s) 46
Hin6I GCGC 1 cut(s) 60
HinP1I GCGC 1 cut(s) 60
HphI GGTGA 1 cut(s) 299
Hpy188I TCNGA 1 cut(s) 141
Hpy188III TCNNGA 4 cut(s) 43, 187, 301, 402
HpyAV CCTTC 2 cut(s) 109, 273
HpyCH4III ACNGT 1 cut(s) 247
HpyCH4V TGCA 1 cut(s) 22
HpyF10VI GCNNNNNNNGC 3 cut(s) 28, 31, 105
HpyF3I CTNAG 1 cut(s) 138
Hsp92II CATG 1 cut(s) 46
HspAI GCGC 1 cut(s) 60
Kzo9I GATC 2 cut(s) 183, 259
LmnI GCTCC 1 cut(s) 404
LpnPI CCDG 5 cut(s) 107, 180, 241, 267, 327
Lsp1109I GCAGC 4 cut(s) 34, 37, 40, 43
MaeI CTAG 2 cut(s) 224, 296
MaeIII GTNAC 1 cut(s) 178
MalI GATC 2 cut(s) 185, 261
MboI GATC 2 cut(s) 183, 259
MboII GAAGA 3 cut(s) 64, 263, 334
MfeI CAATTG 1 cut(s) 126
MlsI TGGCCA 1 cut(s) 309
MluCI AATT 2 cut(s) 126, 390
MluNI TGGCCA 1 cut(s) 309
MmeI TCCRAC 1 cut(s) 67
MnlI CCTC 4 cut(s) 80, 83, 323, 394
Mox20I TGGCCA 1 cut(s) 309
MscI TGGCCA 1 cut(s) 309
MseI TTAA 2 cut(s) 219, 272
Msp20I TGGCCA 1 cut(s) 309
MunI CAATTG 1 cut(s) 126
MvnI CGCG 1 cut(s) 188
MwoI GCNNNNNNNGC 3 cut(s) 28, 31, 105
NdeII GATC 2 cut(s) 183, 259
NlaIII CATG 1 cut(s) 46
NmuCI GTSAC 1 cut(s) 178
NruI TCGCGA 1 cut(s) 188
NsbI TGCGCA 1 cut(s) 61
PagI TCATGA 1 cut(s) 42
PkrI GCNGC 4 cut(s) 24, 27, 30, 33
Ple19I CGATCG 1 cut(s) 186
PvuI CGATCG 1 cut(s) 186
RruI TCGCGA 1 cut(s) 188
SaqAI TTAA 2 cut(s) 219, 272
SatI GCNGC 4 cut(s) 23, 26, 29, 32
Sau3AI GATC 2 cut(s) 183, 259
SetI ASST 3 cut(s) 36, 316, 334
Sse9I AATT 2 cut(s) 126, 390
SspMI CTAG 2 cut(s) 224, 296
TaaI ACNGT 1 cut(s) 247
TaqII GACCGA 2 cut(s) 65, 142
TasI AATT 2 cut(s) 126, 390
Tru1I TTAA 2 cut(s) 219, 272
Tru9I TTAA 2 cut(s) 219, 272
TscAI CASTG 1 cut(s) 101
TseFI GTSAC 1 cut(s) 178
TseI GCWGC 4 cut(s) 22, 25, 28, 31
Tsp45I GTSAC 1 cut(s) 178
TspDTI ATGAA 3 cut(s) 17, 59, 351
TspRI CASTG 1 cut(s) 101
XspI CTAG 2 cut(s) 224, 296
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.