FvH4_2g28701

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0001875
Physical Location & Seq
Reverse (-)
22433992 .. 22434647
656 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g28701.t1

Sequence Viewer

Length: 375 bp
ATGTCGGATCTTGTGAAGTTTCTTATCCTGGCCTTACTTTGCAGTGTCATTCTAGGAGGTGAATGTGATGATTGCAGCTCGCAAAAGCTTCAGGTTGCGCAGACGAAAACTGGAAAGCTAGTAGAAAATAAGCCAGAGTGGAAAGTAACCATCACGAACACCTGCAGTACTTGTTCTCAACTGGCTATTCAACTTAGCTGCAATGGGTTTCAAACTGTTGAAGACGTTGATCCTTCAATCCTAGGGCCATCTGGTGGCATTTGTTACCTTAACAGTGGTAACGTAATACCTGGCCATCAGAGTTTCACCTTCACATATGCTTGGGACGCTGCCTTTCCTTTCGAGGCAGTTTTTTCTCAAGCTCTTTGTTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

125

Amino Acids

13.31

Weight (kDa)

4.5

Isoelectric Point (pI)

40.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPD1_C PF24068 27 - 119 9.1e-29 Tapetum determinant 1, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 170
Acc16I TGCGCA 1 cut(s) 99
Acc36I ACCTGC 1 cut(s) 170
AccB7I CCANNNNNTGG 1 cut(s) 254
AclWI GGATC 2 cut(s) 15, 224
AcoI YGGCCR 1 cut(s) 292
AcuI CTGAAG 1 cut(s) 74
AfaI GTAC 1 cut(s) 169
AfiI CCNNNNNNNGG 1 cut(s) 254
AgsI TTSAA 4 cut(s) 191, 212, 221, 237
AjnI CCWGG 2 cut(s) 27, 289
AluBI AGCT 5 cut(s) 78, 88, 118, 198, 362
AluI AGCT 5 cut(s) 78, 88, 118, 198, 362
AlwI GGATC 2 cut(s) 15, 224
AoxI GGCC 3 cut(s) 30, 245, 292
ApeKI GCWGC 3 cut(s) 75, 198, 329
AspA2I CCTAGG 1 cut(s) 241
AspLEI GCGC 1 cut(s) 100
AspS9I GGNCC 1 cut(s) 245
AsuHPI GGTGA 2 cut(s) 71, 298
AvrII CCTAGG 1 cut(s) 241
BalI TGGCCA 1 cut(s) 294
BbsI GAAGAC 1 cut(s) 228
BbvI GCAGC 3 cut(s) 87, 185, 316
BccI CCATC 3 cut(s) 158, 256, 303
BciT130I CCWGG 2 cut(s) 29, 291
BfaI CTAG 3 cut(s) 53, 119, 242
BfmI CTRYAG 1 cut(s) 163
BfuAI ACCTGC 1 cut(s) 170
BisI GCNGC 3 cut(s) 76, 199, 330
BlnI CCTAGG 1 cut(s) 241
BlsI GCNGC 3 cut(s) 77, 200, 331
BmcAI AGTACT 1 cut(s) 169
Bme1390I CCNGG 2 cut(s) 29, 291
BmgT120I GGNCC 1 cut(s) 245
BmrFI CCNGG 2 cut(s) 29, 291
BpiI GAAGAC 1 cut(s) 228
BpuEI CTTGAG 1 cut(s) 342
BsaJI CCNNGG 1 cut(s) 241
Bsc4I CCNNNNNNNGG 1 cut(s) 254
Bse1I ACTGG 2 cut(s) 115, 186
Bse3DI GCAATG 1 cut(s) 208
BseBI CCWGG 2 cut(s) 29, 291
BseDI CCNNGG 1 cut(s) 241
BseLI CCNNNNNNNGG 1 cut(s) 254
BseMI GCAATG 1 cut(s) 208
BseNI ACTGG 2 cut(s) 115, 186
BseXI GCAGC 3 cut(s) 87, 185, 316
BshFI GGCC 3 cut(s) 32, 247, 294
BslFI GGGAC 1 cut(s) 338
BslI CCNNNNNNNGG 1 cut(s) 254
BsmFI GGGAC 1 cut(s) 338
BsnI GGCC 3 cut(s) 32, 247, 294
Bsp143I GATC 2 cut(s) 7, 229
BspANI GGCC 3 cut(s) 32, 247, 294
BspMAI CTGCAG 1 cut(s) 167
BspMI ACCTGC 1 cut(s) 170
BspPI GGATC 2 cut(s) 15, 224
BsrDI GCAATG 1 cut(s) 208
BsrI ACTGG 2 cut(s) 115, 186
BssECI CCNNGG 1 cut(s) 241
BssMI GATC 2 cut(s) 7, 229
BssT1I CCWWGG 1 cut(s) 241
Bst2UI CCWGG 2 cut(s) 29, 291
Bst4CI ACNGT 2 cut(s) 217, 275
BstC8I GCNNGC 1 cut(s) 80
BstDEI CTNAG 1 cut(s) 194
BstHHI GCGC 1 cut(s) 100
BstKTI GATC 2 cut(s) 10, 232
BstMBI GATC 2 cut(s) 7, 229
BstMWI GCNNNNNNNGC 1 cut(s) 326
BstNI CCWGG 2 cut(s) 29, 291
BstSCI CCNGG 2 cut(s) 27, 289
BstSFI CTRYAG 1 cut(s) 163
BstV1I GCAGC 3 cut(s) 87, 185, 316
BstV2I GAAGAC 1 cut(s) 228
BstX2I RGATCY 1 cut(s) 7
BstYI RGATCY 1 cut(s) 7
BsuRI GGCC 3 cut(s) 32, 247, 294
BtsI GCAGTG 1 cut(s) 49
BtsIMutI CAGTG 2 cut(s) 49, 280
BveI ACCTGC 1 cut(s) 170
Cac8I GCNNGC 1 cut(s) 80
CfoI GCGC 1 cut(s) 100
Cfr13I GGNCC 1 cut(s) 245
CseI GACGC 1 cut(s) 335
Csp6I GTAC 1 cut(s) 168
CviQI GTAC 1 cut(s) 168
DdeI CTNAG 1 cut(s) 194
DpnI GATC 2 cut(s) 9, 231
DpnII GATC 2 cut(s) 7, 229
EaeI YGGCCR 1 cut(s) 292
Eco130I CCWWGG 1 cut(s) 241
Eco57I CTGAAG 1 cut(s) 74
EcoRII CCWGG 2 cut(s) 27, 289
EcoT14I CCWWGG 1 cut(s) 241
ErhI CCWWGG 1 cut(s) 241
FaiI YATR 2 cut(s) 316, 318
FaqI GGGAC 1 cut(s) 338
FauNDI CATATG 1 cut(s) 316
Fnu4HI GCNGC 3 cut(s) 76, 199, 330
Fsp4HI GCNGC 3 cut(s) 76, 199, 330
FspBI CTAG 3 cut(s) 53, 119, 242
FspI TGCGCA 1 cut(s) 99
GlaI GCGC 1 cut(s) 99
GluI GCNGC 3 cut(s) 76, 199, 330
HaeIII GGCC 3 cut(s) 32, 247, 294
HgaI GACGC 1 cut(s) 335
HhaI GCGC 1 cut(s) 100
Hin6I GCGC 1 cut(s) 98
HinP1I GCGC 1 cut(s) 98
HindIII AAGCTT 1 cut(s) 86
HphI GGTGA 2 cut(s) 71, 298
Hpy188I TCNGA 2 cut(s) 7, 300
Hpy188III TCNNGA 1 cut(s) 154
HpyAV CCTTC 2 cut(s) 243, 319
HpyCH4III ACNGT 2 cut(s) 217, 275
HpyCH4IV ACGT 2 cut(s) 225, 282
HpyCH4V TGCA 4 cut(s) 42, 75, 165, 201
HpyF10VI GCNNNNNNNGC 1 cut(s) 326
HpyF3I CTNAG 1 cut(s) 194
HpySE526I ACGT 2 cut(s) 225, 282
HspAI GCGC 1 cut(s) 98
Kzo9I GATC 2 cut(s) 7, 229
Lsp1109I GCAGC 3 cut(s) 87, 185, 316
MaeI CTAG 3 cut(s) 53, 119, 242
MaeII ACGT 2 cut(s) 225, 282
MaeIII GTNAC 3 cut(s) 145, 263, 278
MalI GATC 2 cut(s) 9, 231
MboI GATC 2 cut(s) 7, 229
MboII GAAGA 1 cut(s) 233
MflI RGATCY 1 cut(s) 7
MlsI TGGCCA 1 cut(s) 294
MluNI TGGCCA 1 cut(s) 294
MnlI CCTC 2 cut(s) 50, 337
Mox20I TGGCCA 1 cut(s) 294
MscI TGGCCA 1 cut(s) 294
MseI TTAA 2 cut(s) 270, 373
Msp20I TGGCCA 1 cut(s) 294
MspR9I CCNGG 2 cut(s) 29, 291
MvaI CCWGG 2 cut(s) 29, 291
MwoI GCNNNNNNNGC 1 cut(s) 326
NdeI CATATG 1 cut(s) 316
NdeII GATC 2 cut(s) 7, 229
NsbI TGCGCA 1 cut(s) 99
PaqCI CACCTGC 1 cut(s) 170
PflMI CCANNNNNTGG 1 cut(s) 254
PkrI GCNGC 3 cut(s) 77, 200, 331
Psp6I CCWGG 2 cut(s) 27, 289
PspGI CCWGG 2 cut(s) 27, 289
PspPI GGNCC 1 cut(s) 245
PstI CTGCAG 1 cut(s) 167
PsuI RGATCY 1 cut(s) 7
RsaI GTAC 1 cut(s) 169
RsaNI GTAC 1 cut(s) 168
SaqAI TTAA 2 cut(s) 270, 373
SatI GCNGC 3 cut(s) 76, 199, 330
Sau3AI GATC 2 cut(s) 7, 229
Sau96I GGNCC 1 cut(s) 245
ScaI AGTACT 1 cut(s) 169
ScrFI CCNGG 2 cut(s) 29, 291
SfcI CTRYAG 1 cut(s) 163
SmlI CTYRAG 1 cut(s) 357
SmoI CTYRAG 1 cut(s) 357
SspMI CTAG 3 cut(s) 53, 119, 242
StyD4I CCNGG 2 cut(s) 27, 289
StyI CCWWGG 1 cut(s) 241
TaaI ACNGT 2 cut(s) 217, 275
TaiI ACGT 2 cut(s) 228, 285
TaqI TCGA 1 cut(s) 342
TatI WGTACW 1 cut(s) 167
Tru1I TTAA 2 cut(s) 270, 373
Tru9I TTAA 2 cut(s) 270, 373
TscAI CASTG 2 cut(s) 49, 280
TseI GCWGC 3 cut(s) 75, 198, 329
TspRI CASTG 2 cut(s) 49, 280
Van91I CCANNNNNTGG 1 cut(s) 254
XmaJI CCTAGG 1 cut(s) 241
XspI CTAG 3 cut(s) 53, 119, 242
ZrmI AGTACT 1 cut(s) 169
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.