AT5G28520

Jacalin-related lectin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0000646
Physical Location & Seq
Forward (+)
10492977 .. 10495011
2035 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G28520.1

Sequence Viewer

Length: 1362 bp
ATGGCTCAGAAGTTAGAAGCAATAGGTAGAGGCCTCCAGTGGGATGATTCATCTGACCATGACAATGTAACAAAGATTCTTGTACGAGGTGGTCGTGAAGGCATTCAGTACGTCAAGTTTGATTATGTCAAGAGTGGGCAACCCCAAACTGGATTAATCCATGGTCTCTCGGGTAGAGGAGGATTCACACAAACGTTTGAGATTGATCAAAAGGATGAACATCTAGTATCTGTTGAGGGCTACTATGATGTCACTAAGGGTGTGATCCAAGCACTTAAATTCAAAACCAACAAGAAGACTTCGGAGATGATAGGATACGATGACACGGGTATTAAGCTTTCACTTGAAGTCAAGGGTAAAAAGATAATTGGCTTCCATGGATATGCCGAGACTAACTTAAACTCTCTTGGAGCTTATTTTACAACGACTGGTCCTATTGGATTGAACCCCCAAGTTGGTCATATTAAATTGGCCTATCAAGGTGGTGGCGGAGGGATCCCTTGGGATCATGGTCCTAATCACAACGGTGTGAAAAGGGTTAGCTTTATCTTTGATGAAAACGAGATAAGGCAATGGAGGGTCGACTATGACGATGGTGGAGTGATAAGACAATATGAGCCGATCAATGGATATGATATGTTTGAGGTCAAAGAATATCCAACTGAATACATCATATCGGTGGAGTGTACCTACGATGATGTAATACCTAGAAGTGGCAGGCGTATGATCAGGTCAATAATGTTCAAAACTTCAAAAGGGAGAGTCTCTCCGATATTTGGATACCCTGCTGCTCGCAAATTTGTGCTCGAAAACAACGGTGGTGCTCTTATCGGCTTCCATGGAAGAGTTGGTGCTGGTATAGATGCCCTCGGAGCTTATTTCTCTTCGTTTATTCCTTCTCCGCCTCCTCCTTCCCCCGAAAAACTACAACCAGAAGGTGGTGAGGCAGTAGGAGATCCATGGGACGATGGTATTTTCAATGGTGTGAGAGAGATACATCTAGAAGACGGTGAGGGTATCGCCCTCAAGTTTGTGTACGATAAGGACGTCCAGGTGACTGAACTGAAAGTTCATGGGGAACCATCAGGAATAGGATTCAATGAGTTCAAGCTAGATTATCCGAGTGAATACATCACTACGGTGGAAGGTTTTTGGGACAAAACAAGTGGAAATGAACGCGGAGTTATAACCAGGCTTCGGTTCACGACCAACAAACAAACCTTTCGACCGGTTGGATTGGAGTCGACAACAAGCTTCAGTCTCGGGAAGGAAGGTTACAAGATTGTAGGGTTCCACGGAAATTCTAGTACTGACAAGCTTCACCAGCTTGGGGTCTATGTCGTGCCCATCACGCGCGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Jacalin TIR

Protein Analysis

453

Amino Acids

50.2

Weight (kDa)

5.9

Isoelectric Point (pI)

28.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Jacalin PF01419 10 - 139 3.7e-33 Jacalin-like lectin domain
Jacalin PF01419 160 - 293 1.7e-23 Jacalin-like lectin domain
Jacalin PF01419 311 - 446 4.9e-33 Jacalin-like lectin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1187
AatII GACGTC 1 cut(s) 1050
AccB7I CCANNNNNTGG 1 cut(s) 938
AccI GTMKAC 2 cut(s) 582, 1244
AccII CGCG 3 cut(s) 1179, 1354, 1356
AciI CCGC 3 cut(s) 489, 902, 1179
AclI AACGTT 1 cut(s) 194
AclWI GGATC 5 cut(s) 259, 490, 503, 513, 950
AcsI RAATTY 3 cut(s) 278, 797, 1300
AcuI CTGAAG 1 cut(s) 1240
AcyI GRCGYC 1 cut(s) 1047
AfaI GTAC 5 cut(s) 84, 110, 688, 1037, 1309
AfiI CCNNNNNNNGG 9 cut(s) 40, 149, 455, 626, 713, 776, 938, 1197, 1330
AgeI ACCGGT 1 cut(s) 1228
AgsI TTSAA 8 cut(s) 283, 347, 445, 745, 753, 979, 1099, 1108
AjnI CCWGG 2 cut(s) 1050, 1190
AleI CACNNNNGTG 2 cut(s) 525, 1139
AluBI AGCT 8 cut(s) 337, 413, 543, 875, 1111, 1254, 1318, 1327
AluI AGCT 8 cut(s) 337, 413, 543, 875, 1111, 1254, 1318, 1327
Alw21I GWGCWC 2 cut(s) 807, 826
Alw26I GTCTC 4 cut(s) 170, 383, 769, 1265
AlwI GGATC 5 cut(s) 259, 490, 503, 513, 950
Ama87I CYCGRG 2 cut(s) 169, 1262
AoxI GGCC 2 cut(s) 31, 471
ApeKI GCWGC 1 cut(s) 788
ApoI RAATTY 3 cut(s) 278, 797, 1300
AseI ATTAAT 1 cut(s) 155
AsiGI ACCGGT 1 cut(s) 1228
Asp700I GAANNNNTTC 1 cut(s) 102
AspLEI GCGC 1 cut(s) 1356
AspS9I GGNCC 2 cut(s) 431, 512
AsuHPI GGTGA 4 cut(s) 953, 1022, 1066, 1313
AvaI CYCGRG 2 cut(s) 169, 1262
AvaII GGWCC 2 cut(s) 431, 512
BaeGI GKGCMC 1 cut(s) 1347
BamHI GGATCC 1 cut(s) 495
BarI GAAGNNNNNNTAC 2 cut(s) 1259, 1291
BbsI GAAGAC 2 cut(s) 302, 1011
Bbv12I GWGCWC 2 cut(s) 807, 826
BbvI GCAGC 1 cut(s) 775
BccI CCATC 4 cut(s) 587, 962, 1090, 1355
BciT130I CCWGG 2 cut(s) 1052, 1192
BciVI GTATCC 2 cut(s) 308, 773
BclI TGATCA 2 cut(s) 205, 726
BcoDI GTCTC 4 cut(s) 170, 383, 769, 1265
BfaI CTAG 5 cut(s) 224, 708, 1001, 1112, 1305
BfuI GTATCC 2 cut(s) 308, 773
BisI GCNGC 1 cut(s) 789
BlsI GCNGC 1 cut(s) 790
BmcAI AGTACT 1 cut(s) 1309
Bme1390I CCNGG 2 cut(s) 1052, 1192
Bme18I GGWCC 2 cut(s) 431, 512
BmeT110I CYCGRG 2 cut(s) 169, 1262
BmgT120I GGNCC 2 cut(s) 431, 512
BmiI GGNNCC 3 cut(s) 497, 1080, 1292
BmrFI CCNGG 2 cut(s) 1052, 1192
BmsI GCATC 1 cut(s) 853
BpiI GAAGAC 2 cut(s) 302, 1011
BplI GAGNNNNNCTC 2 cut(s) 751, 783
BpmI CTGGAG 1 cut(s) 20
BpuEI CTTGAG 1 cut(s) 1010
BsaBI GATNNNNATC 1 cut(s) 219
BsaHI GRCGYC 1 cut(s) 1047
BsaI GGTCTC 1 cut(s) 170
BsaJI CCNNGG 7 cut(s) 160, 376, 500, 838, 868, 959, 1294
BsaWI WCCGGW 1 cut(s) 1228
Bsc4I CCNNNNNNNGG 9 cut(s) 40, 149, 455, 626, 713, 776, 938, 1197, 1330
Bse118I RCCGGY 1 cut(s) 1228
Bse1I ACTGG 3 cut(s) 37, 154, 433
Bse3DI GCAATG 1 cut(s) 578
Bse8I GATNNNNATC 1 cut(s) 219
BseBI CCWGG 2 cut(s) 1052, 1192
BseDI CCNNGG 7 cut(s) 160, 376, 500, 838, 868, 959, 1294
BseGI GGATG 2 cut(s) 49, 220
BseJI GATNNNNATC 1 cut(s) 219
BseLI CCNNNNNNNGG 9 cut(s) 40, 149, 455, 626, 713, 776, 938, 1197, 1330
BseMI GCAATG 1 cut(s) 578
BseMII CTCAG 1 cut(s) 20
BseNI ACTGG 3 cut(s) 37, 154, 433
BseRI GAGGAG 2 cut(s) 192, 897
BseSI GKGCMC 1 cut(s) 1347
BseXI GCAGC 1 cut(s) 775
Bsh1236I CGCG 3 cut(s) 1179, 1354, 1356
Bsh1285I CGRYCG 1 cut(s) 1229
BshFI GGCC 2 cut(s) 33, 473
BshTI ACCGGT 1 cut(s) 1228
BsiEI CGRYCG 1 cut(s) 1229
BsiHKAI GWGCWC 2 cut(s) 807, 826
BsiHKCI CYCGRG 2 cut(s) 169, 1262
BsiSI CCGG 1 cut(s) 1229
BslFI GGGAC 2 cut(s) 977, 1169
BslI CCNNNNNNNGG 9 cut(s) 40, 149, 455, 626, 713, 776, 938, 1197, 1330
BsmAI GTCTC 4 cut(s) 170, 383, 769, 1265
BsmFI GGGAC 2 cut(s) 977, 1169
BsmI GAATGC 1 cut(s) 102
BsnI GGCC 2 cut(s) 33, 473
Bso31I GGTCTC 1 cut(s) 170
BsoBI CYCGRG 2 cut(s) 169, 1262
Bsp1286I GDGCHC 3 cut(s) 807, 826, 1347
Bsp143I GATC 7 cut(s) 205, 264, 495, 505, 621, 726, 955
Bsp19I CCATGG 4 cut(s) 160, 376, 838, 959
BspACI CCGC 3 cut(s) 489, 902, 1179
BspANI GGCC 2 cut(s) 33, 473
BspCNI CTCAG 1 cut(s) 19
BspFNI CGCG 3 cut(s) 1179, 1354, 1356
BspLI GGNNCC 3 cut(s) 497, 1080, 1292
BspPI GGATC 5 cut(s) 259, 490, 503, 513, 950
BspTNI GGTCTC 1 cut(s) 170
BsrDI GCAATG 1 cut(s) 578
BsrFI RCCGGY 1 cut(s) 1228
BsrI ACTGG 3 cut(s) 37, 154, 433
BssAI RCCGGY 1 cut(s) 1228
BssECI CCNNGG 7 cut(s) 160, 376, 500, 838, 868, 959, 1294
BssMI GATC 7 cut(s) 205, 264, 495, 505, 621, 726, 955
BssNI GRCGYC 1 cut(s) 1047
BssT1I CCWWGG 5 cut(s) 160, 376, 500, 838, 959
Bst2UI CCWGG 2 cut(s) 1052, 1192
Bst4CI ACNGT 4 cut(s) 527, 818, 1010, 1141
Bst6I CTCTTC 2 cut(s) 838, 889
BstACI GRCGYC 1 cut(s) 1047
BstC8I GCNNGC 2 cut(s) 719, 793
BstDEI CTNAG 2 cut(s) 6, 255
BstDSI CCRYGG 5 cut(s) 160, 376, 838, 959, 1294
BstF5I GGATG 2 cut(s) 49, 220
BstFNI CGCG 3 cut(s) 1179, 1354, 1356
BstHHI GCGC 1 cut(s) 1356
BstKTI GATC 7 cut(s) 208, 267, 498, 508, 624, 729, 958
BstMAI GTCTC 4 cut(s) 170, 383, 769, 1265
BstMBI GATC 7 cut(s) 205, 264, 495, 505, 621, 726, 955
BstMCI CGRYCG 1 cut(s) 1229
BstMWI GCNNNNNNNGC 3 cut(s) 872, 1324, 1351
BstNI CCWGG 2 cut(s) 1052, 1192
BstSCI CCNGG 2 cut(s) 1050, 1190
BstSLI GKGCMC 1 cut(s) 1347
BstUI CGCG 3 cut(s) 1179, 1354, 1356
BstV1I GCAGC 1 cut(s) 775
BstV2I GAAGAC 2 cut(s) 302, 1011
BstX2I RGATCY 2 cut(s) 495, 955
BstYI RGATCY 2 cut(s) 495, 955
BsuI GTATCC 2 cut(s) 308, 773
BsuRI GGCC 2 cut(s) 33, 473
BtgI CCRYGG 5 cut(s) 160, 376, 838, 959, 1294
BtsCI GGATG 2 cut(s) 49, 220
BtsIMutI CAGTG 1 cut(s) 44
Cac8I GCNNGC 2 cut(s) 719, 793
CfoI GCGC 1 cut(s) 1356
Cfr10I RCCGGY 1 cut(s) 1228
Cfr13I GGNCC 2 cut(s) 431, 512
Csp6I GTAC 5 cut(s) 83, 109, 687, 1036, 1308
CspAI ACCGGT 1 cut(s) 1228
CspCI CAANNNNNGTGG 2 cut(s) 1147, 1182
CviAII CATG 7 cut(s) 59, 161, 377, 509, 839, 960, 1073
CviQI GTAC 5 cut(s) 83, 109, 687, 1036, 1308
DdeI CTNAG 2 cut(s) 6, 255
DpnI GATC 7 cut(s) 207, 266, 497, 507, 623, 728, 957
DpnII GATC 7 cut(s) 205, 264, 495, 505, 621, 726, 955
Eam1104I CTCTTC 2 cut(s) 838, 889
EarI CTCTTC 2 cut(s) 838, 889
EciI GGCGGA 2 cut(s) 504, 891
Eco130I CCWWGG 5 cut(s) 160, 376, 500, 838, 959
Eco147I AGGCCT 1 cut(s) 33
Eco31I GGTCTC 1 cut(s) 170
Eco47I GGWCC 2 cut(s) 431, 512
Eco57I CTGAAG 1 cut(s) 1240
Eco88I CYCGRG 2 cut(s) 169, 1262
EcoRII CCWGG 2 cut(s) 1050, 1190
EcoT14I CCWWGG 5 cut(s) 160, 376, 500, 838, 959
ErhI CCWWGG 5 cut(s) 160, 376, 500, 838, 959
FaeI CATG 7 cut(s) 62, 164, 380, 512, 842, 963, 1076
FaqI GGGAC 2 cut(s) 977, 1169
FatI CATG 7 cut(s) 58, 160, 376, 508, 838, 959, 1072
FbaI TGATCA 2 cut(s) 205, 726
FblI GTMKAC 2 cut(s) 582, 1244
Fnu4HI GCNGC 1 cut(s) 789
FokI GGATG 2 cut(s) 56, 227
Fsp4HI GCNGC 1 cut(s) 789
FspBI CTAG 5 cut(s) 224, 708, 1001, 1112, 1305
GlaI GCGC 1 cut(s) 1355
GluI GCNGC 1 cut(s) 789
GsuI CTGGAG 1 cut(s) 20
HaeIII GGCC 2 cut(s) 33, 473
HapII CCGG 1 cut(s) 1229
HhaI GCGC 1 cut(s) 1356
Hin1I GRCGYC 1 cut(s) 1047
Hin1II CATG 7 cut(s) 62, 164, 380, 512, 842, 963, 1076
Hin6I GCGC 1 cut(s) 1354
HinP1I GCGC 1 cut(s) 1354
HincII GTYRAC 2 cut(s) 583, 1245
HindII GTYRAC 2 cut(s) 583, 1245
HindIII AAGCTT 3 cut(s) 335, 1252, 1316
HinfI GANTC 6 cut(s) 47, 76, 183, 762, 1095, 1241
HpaII CCGG 1 cut(s) 1229
HphI GGTGA 4 cut(s) 953, 1022, 1066, 1313
Hpy166II GTNNAC 5 cut(s) 583, 687, 1036, 1203, 1245
Hpy188I TCNGA 6 cut(s) 9, 55, 304, 771, 872, 1122
Hpy188III TCNNGA 6 cut(s) 95, 130, 1001, 1086, 1204, 1264
Hpy8I GTNNAC 5 cut(s) 583, 687, 1036, 1203, 1245
HpyAV CCTTC 7 cut(s) 92, 906, 921, 929, 1139, 1261, 1265
HpyCH4III ACNGT 4 cut(s) 527, 818, 1010, 1141
HpyCH4IV ACGT 3 cut(s) 111, 194, 1047
HpyF10VI GCNNNNNNNGC 3 cut(s) 872, 1324, 1351
HpyF3I CTNAG 2 cut(s) 6, 255
HpySE526I ACGT 3 cut(s) 111, 194, 1047
Hsp92I GRCGYC 1 cut(s) 1047
Hsp92II CATG 7 cut(s) 62, 164, 380, 512, 842, 963, 1076
HspAI GCGC 1 cut(s) 1354
Ksp22I TGATCA 2 cut(s) 205, 726
Kzo9I GATC 7 cut(s) 205, 264, 495, 505, 621, 726, 955
LmnI GCTCC 2 cut(s) 410, 872
Lsp1109I GCAGC 1 cut(s) 775
LweI GCATC 1 cut(s) 853
MaeI CTAG 5 cut(s) 224, 708, 1001, 1112, 1305
MaeII ACGT 3 cut(s) 111, 194, 1047
MaeIII GTNAC 4 cut(s) 67, 250, 1054, 1274
MalI GATC 7 cut(s) 207, 266, 497, 507, 623, 728, 957
MboI GATC 7 cut(s) 205, 264, 495, 505, 621, 726, 955
MboII GAAGA 4 cut(s) 307, 855, 876, 1016
MflI RGATCY 2 cut(s) 495, 955
MhlI GDGCHC 3 cut(s) 807, 826, 1347
MluCI AATT 5 cut(s) 278, 366, 467, 797, 1300
MlyI GAGTC 2 cut(s) 771, 1250
MmeI TCCRAC 2 cut(s) 683, 1213
MroXI GAANNNNTTC 1 cut(s) 102
MseI TTAA 5 cut(s) 155, 276, 333, 398, 465
MslI CAYNNNNRTG 5 cut(s) 63, 381, 525, 677, 1139
MspI CCGG 1 cut(s) 1229
MspR9I CCNGG 2 cut(s) 1052, 1192
Mva1269I GAATGC 1 cut(s) 102
MvaI CCWGG 2 cut(s) 1052, 1192
MvnI CGCG 3 cut(s) 1179, 1354, 1356
MwoI GCNNNNNNNGC 3 cut(s) 872, 1324, 1351
NcoI CCATGG 4 cut(s) 160, 376, 838, 959
NdeII GATC 7 cut(s) 205, 264, 495, 505, 621, 726, 955
NlaIII CATG 7 cut(s) 62, 164, 380, 512, 842, 963, 1076
NlaIV GGNNCC 3 cut(s) 497, 1080, 1292
NmeAIII GCCGAG 1 cut(s) 412
NmuCI GTSAC 2 cut(s) 250, 1054
OliI CACNNNNGTG 2 cut(s) 525, 1139
PceI AGGCCT 1 cut(s) 33
PcsI WCGNNNNNNNCGW 3 cut(s) 91, 588, 1044
PctI GAATGC 1 cut(s) 102
PdmI GAANNNNTTC 1 cut(s) 102
PfeI GAWTC 4 cut(s) 47, 76, 183, 1095
PflMI CCANNNNNTGG 1 cut(s) 938
PinAI ACCGGT 1 cut(s) 1228
PkrI GCNGC 1 cut(s) 790
PleI GAGTC 2 cut(s) 770, 1249
PpsI GAGTC 2 cut(s) 770, 1249
PshBI ATTAAT 1 cut(s) 155
PsiI TTATAA 1 cut(s) 1187
Psp1406I AACGTT 1 cut(s) 194
Psp6I CCWGG 2 cut(s) 1050, 1190
PspGI CCWGG 2 cut(s) 1050, 1190
PspN4I GGNNCC 3 cut(s) 497, 1080, 1292
PspPI GGNCC 2 cut(s) 431, 512
PsuI RGATCY 2 cut(s) 495, 955
RsaI GTAC 5 cut(s) 84, 110, 688, 1037, 1309
RsaNI GTAC 5 cut(s) 83, 109, 687, 1036, 1308
RseI CAYNNNNRTG 5 cut(s) 63, 381, 525, 677, 1139
SalI GTCGAC 2 cut(s) 581, 1243
SaqAI TTAA 5 cut(s) 155, 276, 333, 398, 465
SatI GCNGC 1 cut(s) 789
Sau3AI GATC 7 cut(s) 205, 264, 495, 505, 621, 726, 955
Sau96I GGNCC 2 cut(s) 431, 512
ScaI AGTACT 1 cut(s) 1309
SchI GAGTC 2 cut(s) 771, 1250
ScrFI CCNGG 2 cut(s) 1052, 1192
SduI GDGCHC 3 cut(s) 807, 826, 1347
SfaNI GCATC 1 cut(s) 853
SinI GGWCC 2 cut(s) 431, 512
SmiMI CAYNNNNRTG 5 cut(s) 63, 381, 525, 677, 1139
SmlI CTYRAG 1 cut(s) 1025
SmoI CTYRAG 1 cut(s) 1025
Sse9I AATT 5 cut(s) 278, 366, 467, 797, 1300
SseBI AGGCCT 1 cut(s) 33
SsiI CCGC 3 cut(s) 489, 902, 1179
SspMI CTAG 5 cut(s) 224, 708, 1001, 1112, 1305
StuI AGGCCT 1 cut(s) 33
StyD4I CCNGG 2 cut(s) 1050, 1190
StyI CCWWGG 5 cut(s) 160, 376, 500, 838, 959
TaaI ACNGT 4 cut(s) 527, 818, 1010, 1141
TaiI ACGT 3 cut(s) 114, 197, 1050
TaqI TCGA 4 cut(s) 582, 807, 1225, 1244
TasI AATT 5 cut(s) 278, 366, 467, 797, 1300
TatI WGTACW 1 cut(s) 1307
TfiI GAWTC 4 cut(s) 47, 76, 183, 1095
Tru1I TTAA 5 cut(s) 155, 276, 333, 398, 465
Tru9I TTAA 5 cut(s) 155, 276, 333, 398, 465
TscAI CASTG 1 cut(s) 44
TseFI GTSAC 2 cut(s) 250, 1054
TseI GCWGC 1 cut(s) 788
Tsp45I GTSAC 2 cut(s) 250, 1054
TspDTI ATGAA 5 cut(s) 39, 231, 570, 1061, 1188
TspGWI ACGGA 1 cut(s) 1311
TspRI CASTG 1 cut(s) 44
Van91I CCANNNNNTGG 1 cut(s) 938
VpaK11BI GGWCC 2 cut(s) 431, 512
VspI ATTAAT 1 cut(s) 155
XapI RAATTY 3 cut(s) 278, 797, 1300
XbaI TCTAGA 1 cut(s) 1000
XcmI CCANNNNNNNNNTGG 1 cut(s) 845
XmiI GTMKAC 2 cut(s) 582, 1244
XmnI GAANNNNTTC 1 cut(s) 102
XspI CTAG 5 cut(s) 224, 708, 1001, 1112, 1305
ZraI GACGTC 1 cut(s) 1048
ZrmI AGTACT 1 cut(s) 1309
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.