AT5G41450

Contains the following InterPro domains Zinc finger, RING-type (InterPro IPR001841), Zinc finger, C3HC4 RING-type (InterPro IPR018957)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0002220
Physical Location & Seq
Reverse (-)
16588600 .. 16589094
495 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G41450.1

Sequence Viewer

Length: 495 bp
ATGATGGAGTCTCCACCGTCAGATAATCTGGATTTTTTTTCGACAGTATTGTTTTTATTTATATATATGGTATTTCCAATTGCCATTACAGTAATCTTTATTTATAAACTTTGCATAGATCTTAGTCAGCAACCTCCTACGGAAATAGCTCGTGAGACACACCAAAATTCTCATCCACCACCTGATCAGCTTCAACAAGACATTGAGACTGGACATGTAACGCTGCCTCAGCCTCAACAAAACATTGCAGTCGGATATATGACATGGATCCATGAAACGACAATTTTAGAGTTTAAAGATATAAAAGAAGGATCCAATAAGATTTTTTGTCCAATATGTCTTGAAGAGTTTGAAGATGGCCATGAGATAATTCGTATAAATATGTGTAGACATGTTTTTCATCGTTTTTGTATTGATCCTTGGCTCAATCAGAATCTAACCTGCCCGAATTGTCGATGCTCCCTTACTGCAAGAAAGAGGAAAGAGGGTGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

164

Amino Acids

19.14

Weight (kDa)

5.3

Isoelectric Point (pI)

54.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 110 - 152 2.3e-13 Ring finger domain
zf-C3HC4_2 PF13923 110 - 151 2e-09 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 110 - 151 2.2e-08 Zinc finger, C3HC4 type (RING finger)
zf-RING_UBOX PF13445 110 - 149 2.3e-07 RING-type zinc-finger
zf-rbx1 PF12678 110 - 152 5.8e-07 RING-H2 zinc finger domain
zf-RING_11 PF17123 110 - 138 2.6e-06 RING-like zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 105
Acc36I ACCTGC 1 cut(s) 449
AccI GTMKAC 1 cut(s) 388
AclWI GGATC 5 cut(s) 262, 275, 306, 319, 410
AcoI YGGCCR 1 cut(s) 358
AcsI RAATTY 1 cut(s) 166
AflIII ACRYGT 2 cut(s) 214, 391
AgsI TTSAA 3 cut(s) 194, 344, 353
AluBI AGCT 2 cut(s) 149, 190
AluI AGCT 2 cut(s) 149, 190
Alw26I GTCTC 3 cut(s) 15, 149, 200
AlwI GGATC 5 cut(s) 262, 275, 306, 319, 410
AoxI GGCC 1 cut(s) 358
ApeKI GCWGC 1 cut(s) 223
ApoI RAATTY 1 cut(s) 166
BalI TGGCCA 1 cut(s) 360
BamHI GGATCC 2 cut(s) 267, 311
BauI CACGAG 1 cut(s) 150
BbvCI CCTCAGC 1 cut(s) 228
BbvI GCAGC 1 cut(s) 210
BccI CCATC 1 cut(s) 350
BclI TGATCA 1 cut(s) 184
BcoDI GTCTC 3 cut(s) 15, 149, 200
BfuAI ACCTGC 1 cut(s) 449
BglII AGATCT 1 cut(s) 118
BisI GCNGC 1 cut(s) 224
BlsI GCNGC 1 cut(s) 225
BmiI GGNNCC 2 cut(s) 269, 313
BmsI GCATC 1 cut(s) 446
Bpu10I CCTNAGC 1 cut(s) 228
BsaJI CCNNGG 1 cut(s) 419
Bse1I ACTGG 1 cut(s) 214
Bse3DI GCAATG 1 cut(s) 243
BseDI CCNNGG 1 cut(s) 419
BseGI GGATG 1 cut(s) 172
BseMI GCAATG 1 cut(s) 243
BseMII CTCAG 1 cut(s) 242
BseNI ACTGG 1 cut(s) 214
BseXI GCAGC 1 cut(s) 210
BshFI GGCC 1 cut(s) 360
BsmAI GTCTC 3 cut(s) 15, 149, 200
BsnI GGCC 1 cut(s) 360
Bsp143I GATC 5 cut(s) 118, 184, 267, 311, 415
BspANI GGCC 1 cut(s) 360
BspCNI CTCAG 1 cut(s) 241
BspLI GGNNCC 2 cut(s) 269, 313
BspMI ACCTGC 1 cut(s) 449
BspPI GGATC 5 cut(s) 262, 275, 306, 319, 410
BsrDI GCAATG 1 cut(s) 243
BsrI ACTGG 1 cut(s) 214
BssECI CCNNGG 1 cut(s) 419
BssMI GATC 5 cut(s) 118, 184, 267, 311, 415
BssSI CACGAG 1 cut(s) 150
BssT1I CCWWGG 1 cut(s) 419
Bst2BI CACGAG 1 cut(s) 150
Bst4CI ACNGT 3 cut(s) 18, 46, 91
Bst6I CTCTTC 1 cut(s) 339
BstDEI CTNAG 2 cut(s) 122, 228
BstF5I GGATG 1 cut(s) 172
BstKTI GATC 5 cut(s) 121, 187, 270, 314, 418
BstMAI GTCTC 3 cut(s) 15, 149, 200
BstMBI GATC 5 cut(s) 118, 184, 267, 311, 415
BstMWI GCNNNNNNNGC 1 cut(s) 229
BstNSI RCATGY 2 cut(s) 218, 395
BstV1I GCAGC 1 cut(s) 210
BstX2I RGATCY 3 cut(s) 118, 267, 311
BstYI RGATCY 3 cut(s) 118, 267, 311
BsuRI GGCC 1 cut(s) 360
BtsCI GGATG 1 cut(s) 172
BveI ACCTGC 1 cut(s) 449
CviAII CATG 5 cut(s) 215, 264, 272, 362, 392
CviJI RGCY 5 cut(s) 149, 190, 232, 360, 424
CviKI_1 RGCY 5 cut(s) 149, 190, 232, 360, 424
DdeI CTNAG 2 cut(s) 122, 228
DpnI GATC 5 cut(s) 120, 186, 269, 313, 417
DpnII GATC 5 cut(s) 118, 184, 267, 311, 415
DraI TTTAAA 1 cut(s) 295
EaeI YGGCCR 1 cut(s) 358
Eam1104I CTCTTC 1 cut(s) 339
EarI CTCTTC 1 cut(s) 339
Eco130I CCWWGG 1 cut(s) 419
EcoT14I CCWWGG 1 cut(s) 419
ErhI CCWWGG 1 cut(s) 419
FaeI CATG 5 cut(s) 218, 267, 275, 365, 395
FatI CATG 5 cut(s) 214, 263, 271, 361, 391
FbaI TGATCA 1 cut(s) 184
FblI GTMKAC 1 cut(s) 388
Fnu4HI GCNGC 1 cut(s) 224
FokI GGATG 1 cut(s) 159
Fsp4HI GCNGC 1 cut(s) 224
GluI GCNGC 1 cut(s) 224
HaeIII GGCC 1 cut(s) 360
Hin1II CATG 5 cut(s) 218, 267, 275, 365, 395
HinfI GANTC 2 cut(s) 8, 433
Hpy166II GTNNAC 1 cut(s) 389
Hpy188I TCNGA 3 cut(s) 22, 254, 432
Hpy188III TCNNGA 3 cut(s) 29, 152, 341
Hpy8I GTNNAC 1 cut(s) 389
HpyAV CCTTC 1 cut(s) 302
HpyCH4III ACNGT 3 cut(s) 18, 46, 91
HpyCH4V TGCA 3 cut(s) 114, 248, 470
HpyF10VI GCNNNNNNNGC 1 cut(s) 229
HpyF3I CTNAG 2 cut(s) 122, 228
Hsp92II CATG 5 cut(s) 218, 267, 275, 365, 395
Ksp22I TGATCA 1 cut(s) 184
Kzo9I GATC 5 cut(s) 118, 184, 267, 311, 415
LmnI GCTCC 1 cut(s) 464
LpnPI CCDG 4 cut(s) 14, 195, 195, 454
Lsp1109I GCAGC 1 cut(s) 210
LweI GCATC 1 cut(s) 446
MaeIII GTNAC 1 cut(s) 217
MalI GATC 5 cut(s) 120, 186, 269, 313, 417
MboI GATC 5 cut(s) 118, 184, 267, 311, 415
MboII GAAGA 2 cut(s) 356, 365
MfeI CAATTG 1 cut(s) 78
MflI RGATCY 3 cut(s) 118, 267, 311
MlsI TGGCCA 1 cut(s) 360
MluCI AATT 5 cut(s) 78, 166, 282, 369, 448
MluNI TGGCCA 1 cut(s) 360
MlyI GAGTC 1 cut(s) 17
MmeI TCCRAC 1 cut(s) 232
MnlI CCTC 5 cut(s) 144, 237, 243, 471, 478
Mox20I TGGCCA 1 cut(s) 360
MscI TGGCCA 1 cut(s) 360
MseI TTAA 1 cut(s) 294
Msp20I TGGCCA 1 cut(s) 360
MunI CAATTG 1 cut(s) 78
MwoI GCNNNNNNNGC 1 cut(s) 229
NdeII GATC 5 cut(s) 118, 184, 267, 311, 415
NlaIII CATG 5 cut(s) 218, 267, 275, 365, 395
NlaIV GGNNCC 2 cut(s) 269, 313
NspI RCATGY 2 cut(s) 218, 395
PciI ACATGT 2 cut(s) 214, 391
PfeI GAWTC 1 cut(s) 433
PkrI GCNGC 1 cut(s) 225
PleI GAGTC 1 cut(s) 16
PpsI GAGTC 1 cut(s) 16
PscI ACATGT 2 cut(s) 214, 391
PsiI TTATAA 1 cut(s) 105
PspN4I GGNNCC 2 cut(s) 269, 313
PsuI RGATCY 3 cut(s) 118, 267, 311
SaqAI TTAA 1 cut(s) 294
SatI GCNGC 1 cut(s) 224
Sau3AI GATC 5 cut(s) 118, 184, 267, 311, 415
SchI GAGTC 1 cut(s) 17
SetI ASST 5 cut(s) 136, 151, 184, 192, 443
SfaNI GCATC 1 cut(s) 446
Sse9I AATT 5 cut(s) 78, 166, 282, 369, 448
StyI CCWWGG 1 cut(s) 419
TaaI ACNGT 3 cut(s) 18, 46, 91
TaqI TCGA 2 cut(s) 41, 454
TasI AATT 5 cut(s) 78, 166, 282, 369, 448
TfiI GAWTC 1 cut(s) 433
Tru1I TTAA 1 cut(s) 294
Tru9I TTAA 1 cut(s) 294
TseI GCWGC 1 cut(s) 223
TspDTI ATGAA 2 cut(s) 288, 389
TspGWI ACGGA 1 cut(s) 155
XapI RAATTY 1 cut(s) 166
XceI RCATGY 2 cut(s) 218, 395
XmiI GTMKAC 1 cut(s) 388
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.