RLG00000016299

ubiquitin-protein transferase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0002220
Physical Location & Seq
Forward (+)
5482172 .. 5482633
462 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000016299

Sequence Viewer

Length: 462 bp
ATGAAGGCCTGCATACGACCAAATCAAGCTGATGATCTTCAATCCGCCAGCCTACTAACAACAATTTCACCGTCACCACAACCAACAACAATTTCACCGTCACCACAACCAACAGCACGACCTATAACCAGTCTCGAAGAGCAGGTGACTGCTTCTATTTTTATTGCACCAGAAGCAGACTCACAGGGACGGGCAGGAGGTTACAAAATTTTTAGCTATAGAAGGGATGAGAAAATTGGGTTCCACGATGGAGAGTGCGTCATTTGCTTGGTAGAGTTTGAGGATGAAGATGAGTGCGCAATGCTTAGTAACTGTAAGCAGATTTATCACAGTGTATGCATTGATAAGGTAAAAGAAAATTCGTGTCCTCTTTGTCGTCAAAGTATCAAAGATGCAAGTATAGAAGAAGAATGGATTCAGCTCGTAAAGTTAAGCAGGAGCCATGCTGTTGCAGATGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

154

Amino Acids

16.89

Weight (kDa)

4.84

Isoelectric Point (pI)

72.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 85 - 126 4.5e-09 Ring finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 133
Acc16I TGCGCA 1 cut(s) 298
Acc36I ACCTGC 1 cut(s) 133
AciI CCGC 1 cut(s) 45
AcsI RAATTY 2 cut(s) 207, 358
AgsI TTSAA 1 cut(s) 41
AjuI GAANNNNNNNTTGG 2 cut(s) 76, 108
AluBI AGCT 3 cut(s) 29, 216, 421
AluI AGCT 3 cut(s) 29, 216, 421
Alw26I GTCTC 1 cut(s) 137
AoxI GGCC 1 cut(s) 6
ApoI RAATTY 2 cut(s) 207, 358
Asp700I GAANNNNTTC 1 cut(s) 414
AspLEI GCGC 1 cut(s) 299
AsuHPI GGTGA 5 cut(s) 60, 66, 87, 93, 157
BccI CCATC 1 cut(s) 242
BcoDI GTCTC 1 cut(s) 137
BfmI CTRYAG 1 cut(s) 217
BfuAI ACCTGC 1 cut(s) 133
BmiI GGNNCC 2 cut(s) 242, 440
BmsI GCATC 2 cut(s) 382, 445
BsaXI ACNNNNNCTCC 2 cut(s) 243, 273
Bse1I ACTGG 1 cut(s) 129
Bse3DI GCAATG 1 cut(s) 306
BseGI GGATG 2 cut(s) 232, 289
BseMI GCAATG 1 cut(s) 306
BseNI ACTGG 1 cut(s) 129
BshFI GGCC 1 cut(s) 8
BslFI GGGAC 1 cut(s) 201
BsmAI GTCTC 1 cut(s) 137
BsmFI GGGAC 1 cut(s) 201
BsnI GGCC 1 cut(s) 8
Bsp143I GATC 1 cut(s) 34
BspACI CCGC 1 cut(s) 45
BspANI GGCC 1 cut(s) 8
BspLI GGNNCC 2 cut(s) 242, 440
BspMI ACCTGC 1 cut(s) 133
BspQI GCTCTTC 1 cut(s) 132
BsrDI GCAATG 1 cut(s) 306
BsrI ACTGG 1 cut(s) 129
BssMI GATC 1 cut(s) 34
Bst4CI ACNGT 4 cut(s) 72, 99, 314, 332
Bst6I CTCTTC 1 cut(s) 132
BstC8I GCNNGC 2 cut(s) 10, 49
BstDEI CTNAG 1 cut(s) 305
BstF5I GGATG 2 cut(s) 232, 289
BstHHI GCGC 1 cut(s) 299
BstKTI GATC 1 cut(s) 37
BstMAI GTCTC 1 cut(s) 137
BstMBI GATC 1 cut(s) 34
BstMWI GCNNNNNNNGC 2 cut(s) 173, 264
BstSFI CTRYAG 1 cut(s) 217
BsuRI GGCC 1 cut(s) 8
BtsCI GGATG 2 cut(s) 232, 289
BtsIMutI CAGTG 1 cut(s) 337
BveI ACCTGC 1 cut(s) 133
Cac8I GCNNGC 2 cut(s) 10, 49
CfoI GCGC 1 cut(s) 299
CseI GACGC 1 cut(s) 247
CviAII CATG 2 cut(s) 443, 459
CviJI RGCY 6 cut(s) 8, 29, 51, 216, 421, 441
CviKI_1 RGCY 6 cut(s) 8, 29, 51, 216, 421, 441
DdeI CTNAG 1 cut(s) 305
DpnI GATC 1 cut(s) 36
DpnII GATC 1 cut(s) 34
Eam1104I CTCTTC 1 cut(s) 132
EarI CTCTTC 1 cut(s) 132
EciI GGCGGA 1 cut(s) 34
Eco147I AGGCCT 1 cut(s) 8
EcoT22I ATGCAT 2 cut(s) 341, 460
FaeI CATG 2 cut(s) 446, 462
FaiI YATR 7 cut(s) 14, 125, 219, 337, 401, 444, 460
FaqI GGGAC 1 cut(s) 201
FatI CATG 2 cut(s) 442, 458
FokI GGATG 2 cut(s) 239, 296
FspI TGCGCA 1 cut(s) 298
GlaI GCGC 1 cut(s) 298
HaeIII GGCC 1 cut(s) 8
HgaI GACGC 1 cut(s) 247
HhaI GCGC 1 cut(s) 299
Hin1II CATG 2 cut(s) 446, 462
Hin6I GCGC 1 cut(s) 297
HinP1I GCGC 1 cut(s) 297
HinfI GANTC 2 cut(s) 179, 415
HphI GGTGA 5 cut(s) 60, 66, 87, 93, 157
Hpy188III TCNNGA 1 cut(s) 134
HpyAV CCTTC 1 cut(s) 216
HpyCH4III ACNGT 4 cut(s) 72, 99, 314, 332
HpyCH4V TGCA 6 cut(s) 12, 167, 339, 395, 452, 458
HpyF10VI GCNNNNNNNGC 2 cut(s) 173, 264
HpyF3I CTNAG 1 cut(s) 305
Hsp92II CATG 2 cut(s) 446, 462
HspAI GCGC 1 cut(s) 297
Kzo9I GATC 1 cut(s) 34
LguI GCTCTTC 1 cut(s) 132
LmnI GCTCC 1 cut(s) 438
LpnPI CCDG 8 cut(s) 22, 61, 128, 142, 170, 180, 183, 421
LweI GCATC 2 cut(s) 382, 445
MaeIII GTNAC 5 cut(s) 72, 99, 145, 200, 308
MalI GATC 1 cut(s) 36
MboI GATC 1 cut(s) 34
MboII GAAGA 5 cut(s) 29, 149, 299, 416, 419
MluCI AATT 5 cut(s) 63, 90, 207, 234, 358
MlyI GAGTC 1 cut(s) 173
MnlI CCTC 3 cut(s) 191, 274, 378
Mph1103I ATGCAT 2 cut(s) 341, 460
MroXI GAANNNNTTC 1 cut(s) 414
MseI TTAA 1 cut(s) 431
MwoI GCNNNNNNNGC 2 cut(s) 173, 264
NdeII GATC 1 cut(s) 34
NlaIII CATG 2 cut(s) 446, 462
NlaIV GGNNCC 2 cut(s) 242, 440
NmuCI GTSAC 3 cut(s) 72, 99, 145
NsbI TGCGCA 1 cut(s) 298
NsiI ATGCAT 2 cut(s) 341, 460
PaqCI CACCTGC 1 cut(s) 133
PceI AGGCCT 1 cut(s) 8
PciSI GCTCTTC 1 cut(s) 132
PdmI GAANNNNTTC 1 cut(s) 414
PfeI GAWTC 1 cut(s) 415
PleI GAGTC 1 cut(s) 173
PpsI GAGTC 1 cut(s) 173
PspN4I GGNNCC 2 cut(s) 242, 440
SapI GCTCTTC 1 cut(s) 132
SaqAI TTAA 1 cut(s) 431
Sau3AI GATC 1 cut(s) 34
SchI GAGTC 1 cut(s) 173
SetI ASST 7 cut(s) 31, 124, 147, 202, 218, 351, 423
SfaNI GCATC 2 cut(s) 382, 445
SfcI CTRYAG 1 cut(s) 217
Sse9I AATT 5 cut(s) 63, 90, 207, 234, 358
SseBI AGGCCT 1 cut(s) 8
SsiI CCGC 1 cut(s) 45
StuI AGGCCT 1 cut(s) 8
TaaI ACNGT 4 cut(s) 72, 99, 314, 332
TaqI TCGA 1 cut(s) 135
TasI AATT 5 cut(s) 63, 90, 207, 234, 358
TfiI GAWTC 1 cut(s) 415
Tru1I TTAA 1 cut(s) 431
Tru9I TTAA 1 cut(s) 431
TscAI CASTG 1 cut(s) 337
TseFI GTSAC 3 cut(s) 72, 99, 145
Tsp45I GTSAC 3 cut(s) 72, 99, 145
TspDTI ATGAA 2 cut(s) 17, 300
TspRI CASTG 1 cut(s) 337
XapI RAATTY 2 cut(s) 207, 358
XmnI GAANNNNTTC 1 cut(s) 414
Zsp2I ATGCAT 2 cut(s) 341, 460
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.