RLG00000016300

ubiquitin-protein transferase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0002220
Physical Location & Seq
Forward (+)
5490712 .. 5492396
1685 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000016300

Sequence Viewer

Length: 666 bp
ATGAAGACGGGTCTTGGAGTTGATGAAGAAATCCATGACGTTGAAACCGGCACACTAACAACAACAACTTCACCACAACCATGCACCACCAGCAGTACGTGCAGCAGCAGAAGGCCGAAAGGACTGAATCTTATGAAATTTTTCTTCACCGAAAGGATGTGGAAGCAGTTGGATTTCACAGCAGATCGACAGTTTGTTATTTGTTTGGGAGAGTTTGAGGAAGATGACGAATGCACGATGCTTAAGAATGTGGTCGTCGCAGTCCTTCTCTGTATTCCATATCGAGCCGTTACCTACATCTACAAGAAGATGAACTGTCTTGGAGTTGATGAAGAAATCCATGATATTGAAACCGGCACGCTACCAACTACAACTCCACCACAGTCACCACAAACAGTGCAGCAACAGAAGCAGCAGAAGGCCCAAAGGGTTGAATCTTATGAAATTTTTCATCACCGAAAGGATGTGGAAGCTGTTGGATTTCACAGCGGAGACTGTGTAATTTGTTTGGGAGAGTTTGAGGAAGACGAAGAGTGCGCGATGCTTAAGAAATGTAAGCACACGTACCACCGTGACTGCATTGATAAGGTGAAAGAAGATTCGTGTCCGCTTTGTCGTCATAGTATCGATATGAAAATAGAGAAGCCTAGCAAATTGTTTTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

222

Amino Acids

25.34

Weight (kDa)

5.41

Isoelectric Point (pI)

57.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 165 - 206 1.4e-10 Ring finger domain
zf-RING_11 PF17123 165 - 194 2.3e-08 RING-like zinc finger
zf-RING_5 PF14634 165 - 207 6.4e-07 zinc-RING finger domain
zf-C3HC4_2 PF13923 166 - 205 6.1e-06 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 539
AciI CCGC 2 cut(s) 489, 608
AcsI RAATTY 2 cut(s) 137, 444
AfaI GTAC 2 cut(s) 97, 566
AflII CTTAAG 2 cut(s) 242, 545
AflIII ACRYGT 1 cut(s) 561
AgsI TTSAA 3 cut(s) 44, 350, 434
AluBI AGCT 1 cut(s) 473
AluI AGCT 1 cut(s) 473
Alw26I GTCTC 1 cut(s) 486
AoxI GGCC 2 cut(s) 113, 420
ApeKI GCWGC 4 cut(s) 102, 105, 400, 412
ApoI RAATTY 2 cut(s) 137, 444
Asp700I GAANNNNTTC 2 cut(s) 140, 447
AspLEI GCGC 1 cut(s) 539
AspS9I GGNCC 1 cut(s) 421
AsuHPI GGTGA 5 cut(s) 63, 139, 378, 446, 601
BbsI GAAGAC 2 cut(s) 11, 531
BbvI GCAGC 4 cut(s) 114, 117, 412, 424
BceAI ACGGC 1 cut(s) 272
BcoDI GTCTC 1 cut(s) 486
BfaI CTAG 1 cut(s) 648
BfrI CTTAAG 2 cut(s) 242, 545
BisI GCNGC 4 cut(s) 103, 106, 401, 413
BlsI GCNGC 4 cut(s) 104, 107, 402, 414
BmgT120I GGNCC 1 cut(s) 421
BmsI GCATC 2 cut(s) 228, 531
BpiI GAAGAC 2 cut(s) 11, 531
Bsa29I ATCGAT 1 cut(s) 627
BsaAI YACGTR 2 cut(s) 99, 564
BsaXI ACNNNNNCTCC 4 cut(s) 358, 388, 483, 513
Bse118I RCCGGY 2 cut(s) 47, 353
BseCI ATCGAT 1 cut(s) 627
BseGI GGATG 2 cut(s) 162, 469
BseXI GCAGC 4 cut(s) 114, 117, 412, 424
BsgI GTGCAG 2 cut(s) 121, 419
Bsh1236I CGCG 1 cut(s) 539
BshFI GGCC 2 cut(s) 115, 422
BshVI ATCGAT 1 cut(s) 627
BsiSI CCGG 2 cut(s) 48, 354
BsmAI GTCTC 1 cut(s) 486
BsmI GAATGC 1 cut(s) 236
BsnI GGCC 2 cut(s) 115, 422
Bsp143I GATC 1 cut(s) 184
BspACI CCGC 2 cut(s) 489, 608
BspANI GGCC 2 cut(s) 115, 422
BspDI ATCGAT 1 cut(s) 627
BspFNI CGCG 1 cut(s) 539
BspTI CTTAAG 2 cut(s) 242, 545
BsrFI RCCGGY 2 cut(s) 47, 353
BssAI RCCGGY 2 cut(s) 47, 353
BssMI GATC 1 cut(s) 184
Bst4CI ACNGT 6 cut(s) 192, 317, 384, 397, 497, 572
Bst6I CTCTTC 1 cut(s) 525
BstAFI CTTAAG 2 cut(s) 242, 545
BstAPI GCANNNNNTGC 1 cut(s) 99
BstBAI YACGTR 2 cut(s) 99, 564
BstC8I GCNNGC 1 cut(s) 359
BstF5I GGATG 2 cut(s) 162, 469
BstFNI CGCG 1 cut(s) 539
BstHHI GCGC 1 cut(s) 539
BstKTI GATC 1 cut(s) 187
BstMAI GTCTC 1 cut(s) 486
BstMBI GATC 1 cut(s) 184
BstMWI GCNNNNNNNGC 3 cut(s) 90, 99, 409
BstUI CGCG 1 cut(s) 539
BstV1I GCAGC 4 cut(s) 114, 117, 412, 424
BstV2I GAAGAC 2 cut(s) 11, 531
Bsu15I ATCGAT 1 cut(s) 627
BsuRI GGCC 2 cut(s) 115, 422
BsuTUI ATCGAT 1 cut(s) 627
BtgZI GCGATG 1 cut(s) 554
BtsCI GGATG 2 cut(s) 162, 469
BtsIMutI CAGTG 1 cut(s) 402
Cac8I GCNNGC 1 cut(s) 359
CfoI GCGC 1 cut(s) 539
Cfr10I RCCGGY 2 cut(s) 47, 353
Cfr13I GGNCC 1 cut(s) 421
ClaI ATCGAT 1 cut(s) 627
Csp6I GTAC 2 cut(s) 96, 565
CviAII CATG 3 cut(s) 35, 81, 341
CviJI RGCY 5 cut(s) 115, 287, 422, 473, 646
CviKI_1 RGCY 5 cut(s) 115, 287, 422, 473, 646
CviQI GTAC 2 cut(s) 96, 565
DpnI GATC 1 cut(s) 186
DpnII GATC 1 cut(s) 184
Eam1104I CTCTTC 1 cut(s) 525
EarI CTCTTC 1 cut(s) 525
FaeI CATG 3 cut(s) 38, 84, 344
FaiI YATR 8 cut(s) 36, 82, 134, 280, 342, 441, 621, 632
FatI CATG 3 cut(s) 34, 80, 340
Fnu4HI GCNGC 4 cut(s) 103, 106, 401, 413
FokI GGATG 2 cut(s) 169, 476
Fsp4HI GCNGC 4 cut(s) 103, 106, 401, 413
FspBI CTAG 1 cut(s) 648
GlaI GCGC 1 cut(s) 538
GluI GCNGC 4 cut(s) 103, 106, 401, 413
HaeIII GGCC 2 cut(s) 115, 422
HapII CCGG 2 cut(s) 48, 354
HhaI GCGC 1 cut(s) 539
Hin1II CATG 3 cut(s) 38, 84, 344
Hin6I GCGC 1 cut(s) 537
HinP1I GCGC 1 cut(s) 537
HinfI GANTC 3 cut(s) 127, 434, 599
HpaII CCGG 2 cut(s) 48, 354
HphI GGTGA 5 cut(s) 63, 139, 378, 446, 601
Hpy99I CGWCG 1 cut(s) 260
HpyAV CCTTC 3 cut(s) 105, 275, 412
HpyCH4III ACNGT 6 cut(s) 192, 317, 384, 397, 497, 572
HpyCH4IV ACGT 3 cut(s) 39, 98, 563
HpyCH4V TGCA 5 cut(s) 84, 102, 234, 400, 579
HpyF10VI GCNNNNNNNGC 3 cut(s) 90, 99, 409
HpySE526I ACGT 3 cut(s) 39, 98, 563
Hsp92II CATG 3 cut(s) 38, 84, 344
HspAI GCGC 1 cut(s) 537
Kzo9I GATC 1 cut(s) 184
LpnPI CCDG 3 cut(s) 61, 103, 367
Lsp1109I GCAGC 4 cut(s) 114, 117, 412, 424
LweI GCATC 2 cut(s) 228, 531
MaeI CTAG 1 cut(s) 648
MaeII ACGT 3 cut(s) 39, 98, 563
MaeIII GTNAC 3 cut(s) 289, 384, 572
MalI GATC 1 cut(s) 186
MboI GATC 1 cut(s) 184
MboII GAAGA 9 cut(s) 16, 38, 136, 233, 319, 344, 536, 542, 608
MluCI AATT 4 cut(s) 137, 444, 501, 653
MmeI TCCRAC 2 cut(s) 150, 457
MnlI CCTC 2 cut(s) 211, 514
MroXI GAANNNNTTC 2 cut(s) 140, 447
MseI TTAA 2 cut(s) 243, 546
MslI CAYNNNNRTG 1 cut(s) 79
MspA1I CMGCKG 1 cut(s) 489
MspCI CTTAAG 2 cut(s) 242, 545
MspI CCGG 2 cut(s) 48, 354
Mva1269I GAATGC 1 cut(s) 236
MvnI CGCG 1 cut(s) 539
MwoI GCNNNNNNNGC 3 cut(s) 90, 99, 409
NdeII GATC 1 cut(s) 184
NlaIII CATG 3 cut(s) 38, 84, 344
NmuCI GTSAC 2 cut(s) 384, 572
PctI GAATGC 1 cut(s) 236
PdmI GAANNNNTTC 2 cut(s) 140, 447
PfeI GAWTC 3 cut(s) 127, 434, 599
PkrI GCNGC 4 cut(s) 104, 107, 402, 414
Ppu21I YACGTR 2 cut(s) 99, 564
PspPI GGNCC 1 cut(s) 421
RsaI GTAC 2 cut(s) 97, 566
RsaNI GTAC 2 cut(s) 96, 565
RseI CAYNNNNRTG 1 cut(s) 79
SaqAI TTAA 2 cut(s) 243, 546
SatI GCNGC 4 cut(s) 103, 106, 401, 413
Sau3AI GATC 1 cut(s) 184
Sau96I GGNCC 1 cut(s) 421
SetI ASST 6 cut(s) 42, 101, 296, 475, 566, 591
SfaNI GCATC 2 cut(s) 228, 531
SmiMI CAYNNNNRTG 1 cut(s) 79
SmlI CTYRAG 2 cut(s) 242, 545
SmoI CTYRAG 2 cut(s) 242, 545
Sse9I AATT 4 cut(s) 137, 444, 501, 653
SsiI CCGC 2 cut(s) 489, 608
SspMI CTAG 1 cut(s) 648
TaaI ACNGT 6 cut(s) 192, 317, 384, 397, 497, 572
TaiI ACGT 3 cut(s) 42, 101, 566
TaqI TCGA 3 cut(s) 187, 283, 627
TasI AATT 4 cut(s) 137, 444, 501, 653
TfiI GAWTC 3 cut(s) 127, 434, 599
Tru1I TTAA 2 cut(s) 243, 546
Tru9I TTAA 2 cut(s) 243, 546
TscAI CASTG 1 cut(s) 402
TseFI GTSAC 2 cut(s) 384, 572
TseI GCWGC 4 cut(s) 102, 105, 400, 412
Tsp45I GTSAC 2 cut(s) 384, 572
TspDTI ATGAA 8 cut(s) 17, 39, 149, 326, 345, 440, 456, 647
TspRI CASTG 1 cut(s) 402
Vha464I CTTAAG 2 cut(s) 242, 545
XapI RAATTY 2 cut(s) 137, 444
XmnI GAANNNNTTC 2 cut(s) 140, 447
XspI CTAG 1 cut(s) 648
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.