Rroxscaffold_2G00149240

ubiquitin-protein transferase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0002220
Physical Location & Seq
Reverse (-)
86879557 .. 86881118
1562 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00149240.1

Sequence Viewer

Length: 525 bp
ATGATATTGAAACCGGCACGCTACCAACAACTCTACCACAATCACCACAAGCAATATTGCAGCAACAGAAGCAGCAGAGGGCTCAAAAGGGTTGAATCTTATGAAATTTTTTGTCACCGAAAGGATGTGGAAGCTGTTGGATTTCACAGCGGAGAGTGTGTTATTTGTCTGGGAGAGTTTGAAGAAGACGACGAGTGCGCGATGCTCAAGAAATGTAAGCACACGTACCACCGTGACTGCATTGATAAGGTGAAAGAAGATTCGTGTCCGCTTTGTCGTCATAGTATCGATAAGCAGACTCACAGGGATGGGCAGGAGGTTACAAAATTTTTAGCTATCGATCGACGGGATGAGAAAATTTGGTTCCCCGATGGAGAGTGCGTCATTTGCTTGGTAGAGTTTAAGGAAGAAGATGAGTGCGCAATGCTTAGTAACTGTAAGCACACTTATGACCGTGTATGCATTGATAAGGTAAAGAAAATTCGTGTCCTCTTTGTTGTCAAAGTATCAAAGATACAAGTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

174

Amino Acids

20.56

Weight (kDa)

7.52

Isoelectric Point (pI)

46.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 52 - 93 5.2e-11 Ring finger domain
zf-RING_11 PF17123 52 - 81 1.4e-08 RING-like zinc finger
zf-RING_5 PF14634 52 - 94 3.7e-07 zinc-RING finger domain
zf-C3HC4_2 PF13923 52 - 92 3e-06 Zinc finger, C3HC4 type (RING finger)
zf-RING_2 PF13639 126 - 159 1.4e-06 Ring finger domain
zf-RING_11 PF17123 126 - 155 6.5e-07 RING-like zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 421
AccII CGCG 1 cut(s) 200
AciI CCGC 2 cut(s) 150, 269
AcsI RAATTY 4 cut(s) 105, 326, 357, 480
AfaI GTAC 1 cut(s) 227
AflIII ACRYGT 1 cut(s) 222
AgsI TTSAA 3 cut(s) 10, 95, 182
AluBI AGCT 2 cut(s) 134, 335
AluI AGCT 2 cut(s) 134, 335
ApeKI GCWGC 2 cut(s) 60, 72
ApoI RAATTY 4 cut(s) 105, 326, 357, 480
AspLEI GCGC 2 cut(s) 200, 422
AsuHPI GGTGA 3 cut(s) 35, 107, 262
BanII GRGCYC 1 cut(s) 84
BbsI GAAGAC 1 cut(s) 192
BbvI GCAGC 2 cut(s) 72, 84
BccI CCATC 2 cut(s) 302, 365
BisI GCNGC 2 cut(s) 61, 73
BlsI GCNGC 2 cut(s) 62, 74
BmiI GGNNCC 1 cut(s) 365
BmsI GCATC 1 cut(s) 192
BpiI GAAGAC 1 cut(s) 192
BpuEI CTTGAG 1 cut(s) 191
Bsa29I ATCGAT 2 cut(s) 288, 339
BsaAI YACGTR 1 cut(s) 225
BsaXI ACNNNNNCTCC 4 cut(s) 144, 174, 366, 396
Bse118I RCCGGY 1 cut(s) 13
Bse3DI GCAATG 1 cut(s) 429
BseCI ATCGAT 2 cut(s) 288, 339
BseGI GGATG 3 cut(s) 130, 313, 355
BseMI GCAATG 1 cut(s) 429
BseXI GCAGC 2 cut(s) 72, 84
Bsh1236I CGCG 1 cut(s) 200
Bsh1285I CGRYCG 1 cut(s) 343
BshVI ATCGAT 2 cut(s) 288, 339
BsiEI CGRYCG 1 cut(s) 343
BsiSI CCGG 1 cut(s) 14
Bsp1286I GDGCHC 1 cut(s) 84
Bsp143I GATC 1 cut(s) 340
BspACI CCGC 2 cut(s) 150, 269
BspDI ATCGAT 2 cut(s) 288, 339
BspFNI CGCG 1 cut(s) 200
BspLI GGNNCC 1 cut(s) 365
BsrDI GCAATG 1 cut(s) 429
BsrFI RCCGGY 1 cut(s) 13
BssAI RCCGGY 1 cut(s) 13
BssMI GATC 1 cut(s) 340
Bst4CI ACNGT 3 cut(s) 233, 437, 455
BstBAI YACGTR 1 cut(s) 225
BstC8I GCNNGC 1 cut(s) 19
BstDEI CTNAG 1 cut(s) 428
BstF5I GGATG 3 cut(s) 130, 313, 355
BstFNI CGCG 1 cut(s) 200
BstHHI GCGC 2 cut(s) 200, 422
BstKTI GATC 1 cut(s) 343
BstMBI GATC 1 cut(s) 340
BstMCI CGRYCG 1 cut(s) 343
BstMWI GCNNNNNNNGC 2 cut(s) 69, 387
BstUI CGCG 1 cut(s) 200
BstV1I GCAGC 2 cut(s) 72, 84
BstV2I GAAGAC 1 cut(s) 192
Bsu15I ATCGAT 2 cut(s) 288, 339
BsuTUI ATCGAT 2 cut(s) 288, 339
BtgZI GCGATG 1 cut(s) 215
BtsCI GGATG 3 cut(s) 130, 313, 355
Cac8I GCNNGC 1 cut(s) 19
CfoI GCGC 2 cut(s) 200, 422
Cfr10I RCCGGY 1 cut(s) 13
ClaI ATCGAT 2 cut(s) 288, 339
CseI GACGC 1 cut(s) 370
Csp6I GTAC 1 cut(s) 226
CviJI RGCY 3 cut(s) 82, 134, 335
CviKI_1 RGCY 3 cut(s) 82, 134, 335
CviQI GTAC 1 cut(s) 226
DdeI CTNAG 1 cut(s) 428
DpnI GATC 1 cut(s) 342
DpnII GATC 1 cut(s) 340
Eco24I GRGCYC 1 cut(s) 84
EcoT22I ATGCAT 1 cut(s) 464
EcoT38I GRGCYC 1 cut(s) 84
FaiI YATR 5 cut(s) 102, 282, 450, 460, 523
Fnu4HI GCNGC 2 cut(s) 61, 73
FokI GGATG 3 cut(s) 137, 320, 362
FriOI GRGCYC 1 cut(s) 84
Fsp4HI GCNGC 2 cut(s) 61, 73
FspI TGCGCA 1 cut(s) 421
GlaI GCGC 2 cut(s) 199, 421
GluI GCNGC 2 cut(s) 61, 73
HapII CCGG 1 cut(s) 14
HgaI GACGC 1 cut(s) 370
HhaI GCGC 2 cut(s) 200, 422
Hin6I GCGC 2 cut(s) 198, 420
HinP1I GCGC 2 cut(s) 198, 420
HinfI GANTC 3 cut(s) 95, 260, 298
HpaII CCGG 1 cut(s) 14
HphI GGTGA 3 cut(s) 35, 107, 262
Hpy188III TCNNGA 1 cut(s) 208
Hpy99I CGWCG 2 cut(s) 194, 348
HpyCH4III ACNGT 3 cut(s) 233, 437, 455
HpyCH4IV ACGT 1 cut(s) 224
HpyCH4V TGCA 3 cut(s) 60, 240, 462
HpyF10VI GCNNNNNNNGC 2 cut(s) 69, 387
HpyF3I CTNAG 1 cut(s) 428
HpySE526I ACGT 1 cut(s) 224
HspAI GCGC 2 cut(s) 198, 420
Kzo9I GATC 1 cut(s) 340
LpnPI CCDG 4 cut(s) 27, 155, 289, 299
Lsp1109I GCAGC 2 cut(s) 72, 84
LweI GCATC 1 cut(s) 192
MaeII ACGT 1 cut(s) 224
MaeIII GTNAC 4 cut(s) 113, 233, 319, 431
MalI GATC 1 cut(s) 342
MboI GATC 1 cut(s) 340
MboII GAAGA 5 cut(s) 194, 197, 269, 419, 422
MhlI GDGCHC 1 cut(s) 84
MluCI AATT 4 cut(s) 105, 326, 357, 480
MlyI GAGTC 1 cut(s) 292
MmeI TCCRAC 1 cut(s) 118
MnlI CCTC 3 cut(s) 71, 310, 500
Mph1103I ATGCAT 1 cut(s) 464
MseI TTAA 1 cut(s) 402
MslI CAYNNNNRTG 2 cut(s) 306, 447
MspA1I CMGCKG 1 cut(s) 150
MspI CCGG 1 cut(s) 14
MvnI CGCG 1 cut(s) 200
MwoI GCNNNNNNNGC 2 cut(s) 69, 387
NdeII GATC 1 cut(s) 340
NlaIV GGNNCC 1 cut(s) 365
NmuCI GTSAC 2 cut(s) 113, 233
NsbI TGCGCA 1 cut(s) 421
NsiI ATGCAT 1 cut(s) 464
PfeI GAWTC 2 cut(s) 95, 260
PkrI GCNGC 2 cut(s) 62, 74
Ple19I CGATCG 1 cut(s) 343
PleI GAGTC 1 cut(s) 292
PpsI GAGTC 1 cut(s) 292
Ppu21I YACGTR 1 cut(s) 225
PspN4I GGNNCC 1 cut(s) 365
PvuI CGATCG 1 cut(s) 343
RsaI GTAC 1 cut(s) 227
RsaNI GTAC 1 cut(s) 226
RseI CAYNNNNRTG 2 cut(s) 306, 447
SaqAI TTAA 1 cut(s) 402
SatI GCNGC 2 cut(s) 61, 73
Sau3AI GATC 1 cut(s) 340
SchI GAGTC 1 cut(s) 292
SduI GDGCHC 1 cut(s) 84
SetI ASST 6 cut(s) 136, 227, 252, 321, 337, 474
SfaNI GCATC 1 cut(s) 192
SmiMI CAYNNNNRTG 2 cut(s) 306, 447
SmlI CTYRAG 1 cut(s) 206
SmoI CTYRAG 1 cut(s) 206
Sse9I AATT 4 cut(s) 105, 326, 357, 480
SsiI CCGC 2 cut(s) 150, 269
SspI AATATT 1 cut(s) 56
TaaI ACNGT 3 cut(s) 233, 437, 455
TaiI ACGT 1 cut(s) 227
TaqI TCGA 3 cut(s) 288, 339, 343
TasI AATT 4 cut(s) 105, 326, 357, 480
TfiI GAWTC 2 cut(s) 95, 260
Tru1I TTAA 1 cut(s) 402
Tru9I TTAA 1 cut(s) 402
TseFI GTSAC 2 cut(s) 113, 233
TseI GCWGC 2 cut(s) 60, 72
Tsp45I GTSAC 2 cut(s) 113, 233
TspDTI ATGAA 1 cut(s) 117
XapI RAATTY 4 cut(s) 105, 326, 357, 480
Zsp2I ATGCAT 1 cut(s) 464
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.