Rorug02G0024900

ubiquitin-protein transferase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0002220
Physical Location & Seq
Reverse (-)
1975256 .. 1976098
843 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0024900.1

Sequence Viewer

Length: 843 bp
ATGAGTGAAGGTGCTTTTGAAAGTTTAAATCTTCATCTTCCCTTCAATGGTCAACTACTCAACTATATATTGGCTGGCAATTGCCTCGCGCACATTCTGAGTAGCATTGCAAAAGAAGTACTACTGGAGAATGCCCATGATATAGTTAAGAAAATCCGGGACAGCATCAAGTACGTGACGACTTCAGTGCCCCATGAGGAAAAGTTTCTTGTGCTTAAGAAACAGCTTCAAGTCCCAAGTGAAATAAGGAACATATTTCTTGATGACCAAACTCAGTGGAATACCACATATCAAATGTTAGTGGCCGCTTCTGAGTTGAAGCAAGTTTTTTCTTGCTTGGATACGTATGATTCCGAATTCAACAAACAAGCCCCCTCAATGGAAGATTGGAAGCAAGTTGAGACTCTCTGTGCACATTTGAAGCCAATTTTTGATACAGCATACATCCTAGCTACTACAACTAATCCAACTACAAATATCTTCTTCCATGAAGTGTCGAGGATTCAGGGAGATATGTCTGGTGGTATCACCAATGAGGATCCCTTTGTTAGTAACCTTGCTCGAACAATGCTAGAAAAAAAGAATAGGTACTGGAAGAATTGTAGCCTGACTTTAGCTACAGCAGTGGTCATGGATCCGAGGTACAAGATGAGGTATATTGAGTTCAGTTTCACCAGAATTTATAGCAAAGAAGAAGCTCCAACATGCATGTATCAATTCAGATGGTTGATGATCGAATCCACGAGCTCTATCGATCAGGAATACTTTACAATGCCTTCGCCTCTGGCACCAAACTATGCAGAAGAATTTGATGCGTACATCATGAAGAATTTGATGACATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

280

Amino Acids

32.38

Weight (kDa)

5.33

Isoelectric Point (pI)

53.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
hAT-like_RNase-H PF14372 152 - 237 1.3e-26 hAT-like transposase, RNase-H fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 787
AccII CGCG 1 cut(s) 89
AciI CCGC 1 cut(s) 306
AclWI GGATC 4 cut(s) 533, 546, 629, 642
AcoI YGGCCR 1 cut(s) 303
AcsI RAATTY 4 cut(s) 356, 678, 806, 829
AcuI CTGAAG 1 cut(s) 168
AfaI GTAC 5 cut(s) 120, 173, 590, 644, 818
AfiI CCNNNNNNNGG 2 cut(s) 47, 379
AflII CTTAAG 1 cut(s) 215
AgsI TTSAA 6 cut(s) 20, 46, 230, 319, 361, 421
AluBI AGCT 5 cut(s) 226, 452, 617, 698, 747
AluI AGCT 5 cut(s) 226, 452, 617, 698, 747
Alw21I GWGCWC 2 cut(s) 415, 749
Alw26I GTCTC 1 cut(s) 395
Alw44I GTGCAC 1 cut(s) 411
AlwI GGATC 4 cut(s) 533, 546, 629, 642
AoxI GGCC 1 cut(s) 303
ApaLI GTGCAC 1 cut(s) 411
ApoI RAATTY 4 cut(s) 356, 678, 806, 829
Asp700I GAANNNNTTC 1 cut(s) 204
AspLEI GCGC 1 cut(s) 91
AsuC2I CCSGG 1 cut(s) 158
AsuHPI GGTGA 2 cut(s) 520, 664
BaeGI GKGCMC 2 cut(s) 192, 415
BamHI GGATCC 2 cut(s) 538, 634
BanI GGYRCC 1 cut(s) 787
BanII GRGCYC 1 cut(s) 749
BauI CACGAG 1 cut(s) 742
Bbv12I GWGCWC 2 cut(s) 415, 749
BccI CCATC 1 cut(s) 717
BcgI CGANNNNNNTGC 4 cut(s) 67, 101, 169, 203
BciVI GTATCC 1 cut(s) 334
BcnI CCSGG 1 cut(s) 158
BcoDI GTCTC 1 cut(s) 395
BfaI CTAG 2 cut(s) 449, 572
BfmI CTRYAG 1 cut(s) 618
BfrI CTTAAG 1 cut(s) 215
BfuI GTATCC 1 cut(s) 334
BisI GCNGC 1 cut(s) 306
BlsI GCNGC 1 cut(s) 307
BmcAI AGTACT 1 cut(s) 120
Bme1390I CCNGG 1 cut(s) 158
BmiI GGNNCC 3 cut(s) 540, 636, 789
BmrFI CCNGG 1 cut(s) 158
BmsI GCATC 2 cut(s) 174, 802
BpmI CTGGAG 1 cut(s) 146
BpuMI CCSGG 1 cut(s) 158
Bsa29I ATCGAT 1 cut(s) 753
BsaAI YACGTR 2 cut(s) 175, 345
BsaJI CCNNGG 1 cut(s) 638
Bsc4I CCNNNNNNNGG 2 cut(s) 47, 379
Bse1I ACTGG 2 cut(s) 129, 596
Bse3DI GCAATG 1 cut(s) 105
BseCI ATCGAT 1 cut(s) 753
BseDI CCNNGG 1 cut(s) 638
BseGI GGATG 1 cut(s) 444
BseLI CCNNNNNNNGG 2 cut(s) 47, 379
BseMI GCAATG 1 cut(s) 105
BseMII CTCAG 3 cut(s) 89, 287, 303
BseNI ACTGG 2 cut(s) 129, 596
BseSI GKGCMC 2 cut(s) 192, 415
Bsh1236I CGCG 1 cut(s) 89
BshFI GGCC 1 cut(s) 305
BshNI GGYRCC 1 cut(s) 787
BshVI ATCGAT 1 cut(s) 753
BsiHKAI GWGCWC 2 cut(s) 415, 749
BsiSI CCGG 1 cut(s) 157
BslFI GGGAC 2 cut(s) 173, 218
BslI CCNNNNNNNGG 2 cut(s) 47, 379
BsmAI GTCTC 1 cut(s) 395
BsmFI GGGAC 2 cut(s) 173, 218
BsmI GAATGC 1 cut(s) 136
BsnI GGCC 1 cut(s) 305
Bsp1286I GDGCHC 3 cut(s) 192, 415, 749
Bsp143I GATC 4 cut(s) 538, 634, 732, 754
BspACI CCGC 1 cut(s) 306
BspANI GGCC 1 cut(s) 305
BspCNI CTCAG 3 cut(s) 90, 286, 304
BspDI ATCGAT 1 cut(s) 753
BspFNI CGCG 1 cut(s) 89
BspHI TCATGA 1 cut(s) 822
BspLI GGNNCC 3 cut(s) 540, 636, 789
BspPI GGATC 4 cut(s) 533, 546, 629, 642
BspT107I GGYRCC 1 cut(s) 787
BspTI CTTAAG 1 cut(s) 215
BsrDI GCAATG 1 cut(s) 105
BsrI ACTGG 2 cut(s) 129, 596
BssECI CCNNGG 1 cut(s) 638
BssMI GATC 4 cut(s) 538, 634, 732, 754
BssSI CACGAG 1 cut(s) 742
Bst2BI CACGAG 1 cut(s) 742
BstAFI CTTAAG 1 cut(s) 215
BstBAI YACGTR 2 cut(s) 175, 345
BstC8I GCNNGC 1 cut(s) 76
BstDEI CTNAG 3 cut(s) 98, 273, 312
BstF5I GGATG 1 cut(s) 444
BstFNI CGCG 1 cut(s) 89
BstHHI GCGC 1 cut(s) 91
BstKTI GATC 4 cut(s) 541, 637, 735, 757
BstMAI GTCTC 1 cut(s) 395
BstMBI GATC 4 cut(s) 538, 634, 732, 754
BstNSI RCATGY 2 cut(s) 708, 712
BstSCI CCNGG 1 cut(s) 156
BstSFI CTRYAG 1 cut(s) 618
BstSLI GKGCMC 2 cut(s) 192, 415
BstSNI TACGTA 1 cut(s) 345
BstUI CGCG 1 cut(s) 89
BstX2I RGATCY 2 cut(s) 538, 634
BstYI RGATCY 2 cut(s) 538, 634
Bsu15I ATCGAT 1 cut(s) 753
BsuI GTATCC 1 cut(s) 334
BsuRI GGCC 1 cut(s) 305
BsuTUI ATCGAT 1 cut(s) 753
BtsCI GGATG 1 cut(s) 444
BtsI GCAGTG 1 cut(s) 630
BtsIMutI CAGTG 3 cut(s) 192, 281, 630
Cac8I GCNNGC 1 cut(s) 76
CciI TCATGA 1 cut(s) 822
CfoI GCGC 1 cut(s) 91
ClaI ATCGAT 1 cut(s) 753
Csp6I GTAC 5 cut(s) 119, 172, 589, 643, 817
CspCI CAANNNNNGTGG 2 cut(s) 257, 292
CviAII CATG 7 cut(s) 137, 194, 488, 631, 705, 709, 823
CviQI GTAC 5 cut(s) 119, 172, 589, 643, 817
DdeI CTNAG 3 cut(s) 98, 273, 312
DpnI GATC 4 cut(s) 540, 636, 734, 756
DpnII GATC 4 cut(s) 538, 634, 732, 754
DraI TTTAAA 1 cut(s) 27
EaeI YGGCCR 1 cut(s) 303
Ecl136II GAGCTC 1 cut(s) 747
Eco105I TACGTA 1 cut(s) 345
Eco24I GRGCYC 1 cut(s) 749
Eco53kI GAGCTC 1 cut(s) 747
Eco57I CTGAAG 1 cut(s) 168
EcoICRI GAGCTC 1 cut(s) 747
EcoRI GAATTC 1 cut(s) 356
EcoT22I ATGCAT 1 cut(s) 710
EcoT38I GRGCYC 1 cut(s) 749
FaeI CATG 7 cut(s) 140, 197, 491, 634, 708, 712, 826
FaqI GGGAC 2 cut(s) 173, 218
FatI CATG 7 cut(s) 136, 193, 487, 630, 704, 708, 822
Fnu4HI GCNGC 1 cut(s) 306
FokI GGATG 1 cut(s) 431
FriOI GRGCYC 1 cut(s) 749
Fsp4HI GCNGC 1 cut(s) 306
FspBI CTAG 2 cut(s) 449, 572
GlaI GCGC 1 cut(s) 90
GluI GCNGC 1 cut(s) 306
GsuI CTGGAG 1 cut(s) 146
HaeIII GGCC 1 cut(s) 305
HapII CCGG 1 cut(s) 157
HhaI GCGC 1 cut(s) 91
Hin1II CATG 7 cut(s) 140, 197, 491, 634, 708, 712, 826
Hin6I GCGC 1 cut(s) 89
HinP1I GCGC 1 cut(s) 89
HincII GTYRAC 1 cut(s) 53
HindII GTYRAC 1 cut(s) 53
HinfI GANTC 4 cut(s) 350, 403, 502, 737
HpaII CCGG 1 cut(s) 157
HphI GGTGA 2 cut(s) 520, 664
Hpy166II GTNNAC 2 cut(s) 53, 413
Hpy188I TCNGA 5 cut(s) 99, 313, 355, 639, 722
Hpy188III TCNNGA 3 cut(s) 260, 758, 823
Hpy8I GTNNAC 2 cut(s) 53, 413
HpyAV CCTTC 2 cut(s) 52, 786
HpyCH4IV ACGT 2 cut(s) 174, 344
HpyCH4V TGCA 4 cut(s) 110, 413, 708, 800
HpyF3I CTNAG 3 cut(s) 98, 273, 312
HpySE526I ACGT 2 cut(s) 174, 344
Hsp92II CATG 7 cut(s) 140, 197, 491, 634, 708, 712, 826
HspAI GCGC 1 cut(s) 89
Kzo9I GATC 4 cut(s) 538, 634, 732, 754
LmnI GCTCC 1 cut(s) 703
LweI GCATC 2 cut(s) 174, 802
MaeI CTAG 2 cut(s) 449, 572
MaeII ACGT 2 cut(s) 174, 344
MaeIII GTNAC 2 cut(s) 175, 551
MalI GATC 4 cut(s) 540, 636, 734, 756
MboI GATC 4 cut(s) 538, 634, 732, 754
MboII GAAGA 9 cut(s) 23, 29, 395, 472, 475, 607, 704, 815, 838
MfeI CAATTG 1 cut(s) 79
MflI RGATCY 2 cut(s) 538, 634
MhlI GDGCHC 3 cut(s) 192, 415, 749
MluCI AATT 8 cut(s) 79, 356, 426, 598, 678, 716, 806, 829
MlyI GAGTC 1 cut(s) 397
MmeI TCCRAC 2 cut(s) 491, 725
MnlI CCTC 8 cut(s) 95, 190, 385, 492, 529, 633, 645, 792
Mph1103I ATGCAT 1 cut(s) 710
MroXI GAANNNNTTC 1 cut(s) 204
MseI TTAA 3 cut(s) 26, 147, 216
MspCI CTTAAG 1 cut(s) 215
MspI CCGG 1 cut(s) 157
MspR9I CCNGG 1 cut(s) 158
MunI CAATTG 1 cut(s) 79
Mva1269I GAATGC 1 cut(s) 136
MvnI CGCG 1 cut(s) 89
NciI CCSGG 1 cut(s) 158
NdeII GATC 4 cut(s) 538, 634, 732, 754
NlaIII CATG 7 cut(s) 140, 197, 491, 634, 708, 712, 826
NlaIV GGNNCC 3 cut(s) 540, 636, 789
NmuCI GTSAC 1 cut(s) 175
NsiI ATGCAT 1 cut(s) 710
NspI RCATGY 2 cut(s) 708, 712
PagI TCATGA 1 cut(s) 822
PctI GAATGC 1 cut(s) 136
PdmI GAANNNNTTC 1 cut(s) 204
PfeI GAWTC 3 cut(s) 350, 502, 737
PfoI TCCNGGA 1 cut(s) 156
PkrI GCNGC 1 cut(s) 307
PleI GAGTC 1 cut(s) 397
PpsI GAGTC 1 cut(s) 397
Ppu21I YACGTR 2 cut(s) 175, 345
Psp124BI GAGCTC 1 cut(s) 749
PspN4I GGNNCC 3 cut(s) 540, 636, 789
PsrI GAACNNNNNNTAC 2 cut(s) 647, 679
PsuI RGATCY 2 cut(s) 538, 634
RsaI GTAC 5 cut(s) 120, 173, 590, 644, 818
RsaNI GTAC 5 cut(s) 119, 172, 589, 643, 817
SacI GAGCTC 1 cut(s) 749
SaqAI TTAA 3 cut(s) 26, 147, 216
SatI GCNGC 1 cut(s) 306
Sau3AI GATC 4 cut(s) 538, 634, 732, 754
ScaI AGTACT 1 cut(s) 120
SchI GAGTC 1 cut(s) 397
ScrFI CCNGG 1 cut(s) 158
SduI GDGCHC 3 cut(s) 192, 415, 749
SfaNI GCATC 2 cut(s) 174, 802
SfcI CTRYAG 1 cut(s) 618
SmlI CTYRAG 1 cut(s) 215
SmoI CTYRAG 1 cut(s) 215
SnaBI TACGTA 1 cut(s) 345
Sse9I AATT 8 cut(s) 79, 356, 426, 598, 678, 716, 806, 829
SsiI CCGC 1 cut(s) 306
SspMI CTAG 2 cut(s) 449, 572
SstI GAGCTC 1 cut(s) 749
StyD4I CCNGG 1 cut(s) 156
TaiI ACGT 2 cut(s) 177, 347
TaqI TCGA 4 cut(s) 497, 562, 735, 753
TasI AATT 8 cut(s) 79, 356, 426, 598, 678, 716, 806, 829
TatI WGTACW 1 cut(s) 118
TauI GCSGC 1 cut(s) 308
TfiI GAWTC 3 cut(s) 350, 502, 737
Tru1I TTAA 3 cut(s) 26, 147, 216
Tru9I TTAA 3 cut(s) 26, 147, 216
TscAI CASTG 3 cut(s) 192, 281, 630
TseFI GTSAC 1 cut(s) 175
Tsp45I GTSAC 1 cut(s) 175
TspDTI ATGAA 3 cut(s) 23, 504, 839
TspRI CASTG 3 cut(s) 192, 281, 630
Vha464I CTTAAG 1 cut(s) 215
VneI GTGCAC 1 cut(s) 411
XapI RAATTY 4 cut(s) 356, 678, 806, 829
XceI RCATGY 2 cut(s) 708, 712
XmnI GAANNNNTTC 1 cut(s) 204
XspI CTAG 2 cut(s) 449, 572
ZrmI AGTACT 1 cut(s) 120
Zsp2I ATGCAT 1 cut(s) 710
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.