RchiOBHm_Chr2g0092061

Transmembrane proteins 14C

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0002220
Physical Location & Seq
Forward (+)
5645208 .. 5650967
5760 bp
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UTR
Exon/CDS
Intron
PRQ46722

Sequence Viewer

Length: 696 bp
ATGTGTAAATTAGTCTTATCCACTCAAATCTGTAAAATTATTTTACGAGAAAAAATTTGTTGCCTTCAAGAACCCTACACCTTGGTAACTTTAAATCCTTCTGGCTGCTATAGGCCTCTGATTGATCAGTTAGTCTGTCTCTCTGAAATGACATCGTTTTTGGCCCCTGCTCCTCAACCCTCTCATCAGGGATTAAATCATGGTGAGTTTGGATTTATTATACTCACAATATACATACTAGTTCTTCTCTGCGTCAGAAAACCAGAGTACGTCCTCTATCCTTGTTTAAAGCTTCTGTGTGTCTTCATACTCGGGCCATTTGTTGTGTGCGCCTTCATACTACTCGGGCCAATTTTTGTGTGCTTCAAAATTATACAGACCATCATTGTAGATACGAGACACCAAACAGGTCAAGCTCACCTTGATATCGAATCTGGCGGCCACCAGTTACCAGCCGCTCCACCGCAGCAACCTGCTGGTAACAATGCTGGACAGCCACGACGGCCGAAGGATTGGAGTGGATACTATGAAATTTTTAGTCATCTGAAAGTTGGTGAGTGTAGTATTTGCCTGGGAGAGTTTGAGGAACCAGATGAGTGTGCGAGACTTCGTAAGTGTAAGCATACGTATCACCGGGAATGCATTGATCTGTGGCTACCTAAATCTTGTCCGATTTGTCGTCAAAACGGAGTATAA

Protein Analysis

231

Amino Acids

26.03

Weight (kDa)

7.82

Isoelectric Point (pI)

48.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 186 - 227 6.5e-11 Ring finger domain
zf-RING_11 PF17123 186 - 215 4.7e-09 RING-like zinc finger
zf-RING_5 PF14634 186 - 228 9.9e-07 zinc-RING finger domain
zf-C3HC4_2 PF13923 186 - 226 7.9e-06 Zinc finger, C3HC4 type (RING finger)
zf-RING_UBOX PF13445 187 - 218 1.5e-06 RING-type zinc-finger
zf-C3HC4 PF00097 187 - 226 8.9e-06 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 481
AccBSI CCGCTC 1 cut(s) 458
AciI CCGC 3 cut(s) 438, 456, 464
AcoI YGGCCR 2 cut(s) 439, 503
AcsI RAATTY 2 cut(s) 54, 531
AfaI GTAC 1 cut(s) 269
AgsI TTSAA 2 cut(s) 68, 367
AhlI ACTAGT 1 cut(s) 238
AjnI CCWGG 1 cut(s) 570
AluBI AGCT 2 cut(s) 292, 416
AluI AGCT 2 cut(s) 292, 416
Alw26I GTCTC 3 cut(s) 143, 391, 598
Ama87I CYCGRG 2 cut(s) 311, 344
AoxI GGCC 6 cut(s) 113, 162, 314, 347, 439, 503
ApeKI GCWGC 2 cut(s) 105, 466
ApoI RAATTY 2 cut(s) 54, 531
ArsI GACNNNNNNTTYG 2 cut(s) 142, 174
AspLEI GCGC 1 cut(s) 332
AspS9I GGNCC 3 cut(s) 163, 314, 347
AsuC2I CCSGG 1 cut(s) 635
AsuHPI GGTGA 4 cut(s) 215, 410, 566, 623
AvaI CYCGRG 2 cut(s) 311, 344
BbsI GAAGAC 1 cut(s) 295
BbvI GCAGC 2 cut(s) 92, 478
BccI CCATC 1 cut(s) 389
BceAI ACGGC 1 cut(s) 518
BciT130I CCWGG 1 cut(s) 572
BciVI GTATCC 1 cut(s) 515
BclI TGATCA 1 cut(s) 124
BcnI CCSGG 1 cut(s) 635
BcoDI GTCTC 3 cut(s) 143, 391, 598
BcuI ACTAGT 1 cut(s) 238
BfaI CTAG 1 cut(s) 239
BfmI CTRYAG 1 cut(s) 109
BfuAI ACCTGC 1 cut(s) 481
BfuI GTATCC 1 cut(s) 515
BglI GCCNNNNNGGC 1 cut(s) 502
BisI GCNGC 4 cut(s) 106, 439, 456, 467
BlsI GCNGC 4 cut(s) 107, 440, 457, 468
Bme1390I CCNGG 2 cut(s) 572, 635
BmeT110I CYCGRG 2 cut(s) 311, 344
BmgT120I GGNCC 3 cut(s) 163, 314, 347
BmiI GGNNCC 2 cut(s) 165, 588
BmrFI CCNGG 2 cut(s) 572, 635
BpiI GAAGAC 1 cut(s) 295
BpuMI CCSGG 1 cut(s) 635
BsaAI YACGTR 1 cut(s) 627
BsaJI CCNNGG 2 cut(s) 81, 571
Bse1I ACTGG 1 cut(s) 445
BseBI CCWGG 1 cut(s) 572
BseDI CCNNGG 2 cut(s) 81, 571
BseNI ACTGG 1 cut(s) 445
BseRI GAGGAG 1 cut(s) 162
BseX3I CGGCCG 1 cut(s) 503
BseXI GCAGC 2 cut(s) 92, 478
Bsh1285I CGRYCG 1 cut(s) 506
BshFI GGCC 6 cut(s) 115, 164, 316, 349, 441, 505
BsiEI CGRYCG 1 cut(s) 506
BsiHKCI CYCGRG 2 cut(s) 311, 344
BsiSI CCGG 1 cut(s) 634
BsmAI GTCTC 3 cut(s) 143, 391, 598
BsmI GAATGC 1 cut(s) 644
BsnI GGCC 6 cut(s) 115, 164, 316, 349, 441, 505
BsoBI CYCGRG 2 cut(s) 311, 344
Bsp143I GATC 2 cut(s) 124, 646
BspACI CCGC 3 cut(s) 438, 456, 464
BspANI GGCC 6 cut(s) 115, 164, 316, 349, 441, 505
BspLI GGNNCC 2 cut(s) 165, 588
BspMI ACCTGC 1 cut(s) 481
BsrBI CCGCTC 1 cut(s) 458
BsrI ACTGG 1 cut(s) 445
BssECI CCNNGG 2 cut(s) 81, 571
BssMI GATC 2 cut(s) 124, 646
BssT1I CCWWGG 1 cut(s) 81
Bst2UI CCWGG 1 cut(s) 572
BstBAI YACGTR 1 cut(s) 627
BstHHI GCGC 1 cut(s) 332
BstKTI GATC 2 cut(s) 127, 649
BstMAI GTCTC 3 cut(s) 143, 391, 598
BstMBI GATC 2 cut(s) 124, 646
BstMCI CGRYCG 1 cut(s) 506
BstMWI GCNNNNNNNGC 1 cut(s) 502
BstNI CCWGG 1 cut(s) 572
BstSCI CCNGG 2 cut(s) 570, 633
BstSFI CTRYAG 1 cut(s) 109
BstSNI TACGTA 1 cut(s) 627
BstV1I GCAGC 2 cut(s) 92, 478
BstV2I GAAGAC 1 cut(s) 295
BstZI CGGCCG 1 cut(s) 503
BsuI GTATCC 1 cut(s) 515
BsuRI GGCC 6 cut(s) 115, 164, 316, 349, 441, 505
BveI ACCTGC 1 cut(s) 481
CfoI GCGC 1 cut(s) 332
Cfr13I GGNCC 3 cut(s) 163, 314, 347
CseI GACGC 1 cut(s) 241
Csp6I GTAC 1 cut(s) 268
CviAII CATG 1 cut(s) 200
CviQI GTAC 1 cut(s) 268
DpnI GATC 2 cut(s) 126, 648
DpnII GATC 2 cut(s) 124, 646
DraI TTTAAA 2 cut(s) 93, 288
EaeI YGGCCR 2 cut(s) 439, 503
EagI CGGCCG 1 cut(s) 503
EclXI CGGCCG 1 cut(s) 503
Eco105I TACGTA 1 cut(s) 627
Eco130I CCWWGG 1 cut(s) 81
Eco147I AGGCCT 1 cut(s) 115
Eco32I GATATC 1 cut(s) 427
Eco52I CGGCCG 1 cut(s) 503
Eco88I CYCGRG 2 cut(s) 311, 344
EcoRII CCWGG 1 cut(s) 570
EcoRV GATATC 1 cut(s) 427
EcoT14I CCWWGG 1 cut(s) 81
EcoT22I ATGCAT 1 cut(s) 644
ErhI CCWWGG 1 cut(s) 81
FaeI CATG 1 cut(s) 203
FalI AAGNNNNNCTT 2 cut(s) 405, 437
FatI CATG 1 cut(s) 199
FbaI TGATCA 1 cut(s) 124
Fnu4HI GCNGC 4 cut(s) 106, 439, 456, 467
Fsp4HI GCNGC 4 cut(s) 106, 439, 456, 467
FspBI CTAG 1 cut(s) 239
GlaI GCGC 1 cut(s) 331
GluI GCNGC 4 cut(s) 106, 439, 456, 467
HaeIII GGCC 6 cut(s) 115, 164, 316, 349, 441, 505
HapII CCGG 1 cut(s) 634
HgaI GACGC 1 cut(s) 241
HhaI GCGC 1 cut(s) 332
Hin1II CATG 1 cut(s) 203
Hin6I GCGC 1 cut(s) 330
HinP1I GCGC 1 cut(s) 330
HindIII AAGCTT 1 cut(s) 290
HinfI GANTC 1 cut(s) 431
HpaII CCGG 1 cut(s) 634
HphI GGTGA 4 cut(s) 215, 410, 566, 623
Hpy188I TCNGA 5 cut(s) 120, 145, 257, 546, 672
Hpy188III TCNNGA 1 cut(s) 68
Hpy99I CGWCG 1 cut(s) 504
HpyAV CCTTC 4 cut(s) 74, 108, 343, 502
HpyCH4IV ACGT 2 cut(s) 270, 626
HpyCH4V TGCA 1 cut(s) 642
HpyF10VI GCNNNNNNNGC 1 cut(s) 502
HpySE526I ACGT 2 cut(s) 270, 626
Hsp92II CATG 1 cut(s) 203
HspAI GCGC 1 cut(s) 330
Ksp22I TGATCA 1 cut(s) 124
Kzo9I GATC 2 cut(s) 124, 646
LmnI GCTCC 2 cut(s) 175, 463
Lsp1109I GCAGC 2 cut(s) 92, 478
MaeI CTAG 1 cut(s) 239
MaeII ACGT 2 cut(s) 270, 626
MaeIII GTNAC 3 cut(s) 85, 447, 479
MalI GATC 2 cut(s) 126, 648
MbiI CCGCTC 1 cut(s) 458
MboI GATC 2 cut(s) 124, 646
MboII GAAGA 2 cut(s) 236, 295
MluCI AATT 6 cut(s) 8, 36, 54, 351, 369, 531
MnlI CCTC 5 cut(s) 126, 183, 190, 284, 577
Mph1103I ATGCAT 1 cut(s) 644
MseI TTAA 3 cut(s) 92, 194, 287
MspI CCGG 1 cut(s) 634
MspR9I CCNGG 2 cut(s) 572, 635
Mva1269I GAATGC 1 cut(s) 644
MvaI CCWGG 1 cut(s) 572
MwoI GCNNNNNNNGC 1 cut(s) 502
NciI CCSGG 1 cut(s) 635
NdeII GATC 2 cut(s) 124, 646
NlaIII CATG 1 cut(s) 203
NlaIV GGNNCC 2 cut(s) 165, 588
NsiI ATGCAT 1 cut(s) 644
PceI AGGCCT 1 cut(s) 115
PctI GAATGC 1 cut(s) 644
PfeI GAWTC 1 cut(s) 431
PkrI GCNGC 4 cut(s) 107, 440, 457, 468
Ppu21I YACGTR 1 cut(s) 627
Psp6I CCWGG 1 cut(s) 570
PspGI CCWGG 1 cut(s) 570
PspN4I GGNNCC 2 cut(s) 165, 588
PspPI GGNCC 3 cut(s) 163, 314, 347
RsaI GTAC 1 cut(s) 269
RsaNI GTAC 1 cut(s) 268
SaqAI TTAA 3 cut(s) 92, 194, 287
SatI GCNGC 4 cut(s) 106, 439, 456, 467
Sau3AI GATC 2 cut(s) 124, 646
Sau96I GGNCC 3 cut(s) 163, 314, 347
ScrFI CCNGG 2 cut(s) 572, 635
SetI ASST 9 cut(s) 83, 273, 294, 412, 418, 423, 475, 629, 661
SfcI CTRYAG 1 cut(s) 109
SnaBI TACGTA 1 cut(s) 627
SpeI ACTAGT 1 cut(s) 238
Sse9I AATT 6 cut(s) 8, 36, 54, 351, 369, 531
SseBI AGGCCT 1 cut(s) 115
SsiI CCGC 3 cut(s) 438, 456, 464
SspMI CTAG 1 cut(s) 239
StuI AGGCCT 1 cut(s) 115
StyD4I CCNGG 2 cut(s) 570, 633
StyI CCWWGG 1 cut(s) 81
TaiI ACGT 2 cut(s) 273, 629
TaqI TCGA 1 cut(s) 429
TasI AATT 6 cut(s) 8, 36, 54, 351, 369, 531
TauI GCSGC 2 cut(s) 441, 458
TfiI GAWTC 1 cut(s) 431
Tru1I TTAA 3 cut(s) 92, 194, 287
Tru9I TTAA 3 cut(s) 92, 194, 287
TseI GCWGC 2 cut(s) 105, 466
TspDTI ATGAA 3 cut(s) 295, 325, 543
XapI RAATTY 2 cut(s) 54, 531
XspI CTAG 1 cut(s) 239
Zsp2I ATGCAT 1 cut(s) 644
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.