RchiOBHm_Chr2g0092331

ubiquitin-protein transferase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0002220
Physical Location & Seq
Forward (+)
5800178 .. 5800615
438 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ46744

Sequence Viewer

Length: 438 bp
ATGCATCCGTTTCCCCCCTATCTGGTTTTTGTAGTGGTGGTCGCAGTCCTTCTCTGTATTCCATGTCGAGCCGTTACCTACATCTACAAGAAGATGAAGATGTGTCTTGGAGTTGATGAAGAAATCCATGATATTGAAACCGGCACGCCACCAACAACTCCACCACAATCACCACAAACAGTGCAGCAGAAGGCCCAAAGGGTTGAATCTTATGAAATTTTTCATCACCGAAAGGATGTGGAAGCTGTTGGATTTCACAGCGGAGATTGTGTTATTTGCTTGGGAGAGTTTGAGGAAGACGAAGAGTGCGCGATGCTTAAGAAATGTAAGCACACGTACCACCGTGACTGCATTGATAAGGTGAAAGAAGATTCGTGTCCGCTTTGTCGTCATTGTATCGTCGATGTGAAAATAGAAAAACCTAGCAAATTGTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

145

Amino Acids

16.64

Weight (kDa)

6.05

Isoelectric Point (pI)

57.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 89 - 130 6.9e-11 Ring finger domain
zf-RING_11 PF17123 89 - 118 1.2e-08 RING-like zinc finger
zf-RING_5 PF14634 89 - 131 3e-07 zinc-RING finger domain
zf-C3HC4_2 PF13923 90 - 129 2.9e-06 Zinc finger, C3HC4 type (RING finger)
zf-RING_UBOX PF13445 90 - 125 7.1e-06 RING-type zinc-finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 311
AciI CCGC 2 cut(s) 261, 380
AcsI RAATTY 1 cut(s) 216
AfaI GTAC 1 cut(s) 338
AfiI CCNNNNNNNGG 1 cut(s) 22
AflII CTTAAG 1 cut(s) 317
AflIII ACRYGT 1 cut(s) 333
AgsI TTSAA 2 cut(s) 137, 206
AluBI AGCT 1 cut(s) 245
AluI AGCT 1 cut(s) 245
AoxI GGCC 1 cut(s) 192
ApeKI GCWGC 1 cut(s) 184
ApoI RAATTY 1 cut(s) 216
Asp700I GAANNNNTTC 1 cut(s) 219
AspLEI GCGC 1 cut(s) 311
AspS9I GGNCC 1 cut(s) 193
AsuHPI GGTGA 3 cut(s) 162, 218, 373
BbsI GAAGAC 1 cut(s) 303
BbvI GCAGC 1 cut(s) 196
BceAI ACGGC 1 cut(s) 56
BfaI CTAG 1 cut(s) 423
BfrI CTTAAG 1 cut(s) 317
BisI GCNGC 1 cut(s) 185
BlsI GCNGC 1 cut(s) 186
BmgT120I GGNCC 1 cut(s) 193
BmsI GCATC 2 cut(s) 13, 303
BpiI GAAGAC 1 cut(s) 303
BsaAI YACGTR 1 cut(s) 336
BsaXI ACNNNNNCTCC 2 cut(s) 255, 285
Bsc4I CCNNNNNNNGG 1 cut(s) 22
Bse118I RCCGGY 1 cut(s) 140
BseGI GGATG 2 cut(s) 4, 241
BseLI CCNNNNNNNGG 1 cut(s) 22
BseXI GCAGC 1 cut(s) 196
BsgI GTGCAG 1 cut(s) 203
Bsh1236I CGCG 1 cut(s) 311
BshFI GGCC 1 cut(s) 194
BsiSI CCGG 1 cut(s) 141
BslI CCNNNNNNNGG 1 cut(s) 22
BsnI GGCC 1 cut(s) 194
BspACI CCGC 2 cut(s) 261, 380
BspANI GGCC 1 cut(s) 194
BspFNI CGCG 1 cut(s) 311
BspTI CTTAAG 1 cut(s) 317
BsrFI RCCGGY 1 cut(s) 140
BssAI RCCGGY 1 cut(s) 140
Bst4CI ACNGT 2 cut(s) 181, 344
Bst6I CTCTTC 1 cut(s) 297
BstAFI CTTAAG 1 cut(s) 317
BstBAI YACGTR 1 cut(s) 336
BstC8I GCNNGC 1 cut(s) 146
BstF5I GGATG 2 cut(s) 4, 241
BstFNI CGCG 1 cut(s) 311
BstHHI GCGC 1 cut(s) 311
BstUI CGCG 1 cut(s) 311
BstV1I GCAGC 1 cut(s) 196
BstV2I GAAGAC 1 cut(s) 303
BsuRI GGCC 1 cut(s) 194
BtgZI GCGATG 1 cut(s) 326
BtsCI GGATG 2 cut(s) 4, 241
BtsIMutI CAGTG 1 cut(s) 186
Cac8I GCNNGC 1 cut(s) 146
CfoI GCGC 1 cut(s) 311
Cfr10I RCCGGY 1 cut(s) 140
Cfr13I GGNCC 1 cut(s) 193
Csp6I GTAC 1 cut(s) 337
CviAII CATG 2 cut(s) 63, 128
CviJI RGCY 3 cut(s) 71, 194, 245
CviKI_1 RGCY 3 cut(s) 71, 194, 245
CviQI GTAC 1 cut(s) 337
Eam1104I CTCTTC 1 cut(s) 297
EarI CTCTTC 1 cut(s) 297
EcoT22I ATGCAT 1 cut(s) 6
FaeI CATG 2 cut(s) 66, 131
FaiI YATR 3 cut(s) 64, 129, 213
FatI CATG 2 cut(s) 62, 127
Fnu4HI GCNGC 1 cut(s) 185
FokI GGATG 1 cut(s) 248
Fsp4HI GCNGC 1 cut(s) 185
FspBI CTAG 1 cut(s) 423
GlaI GCGC 1 cut(s) 310
GluI GCNGC 1 cut(s) 185
HaeIII GGCC 1 cut(s) 194
HapII CCGG 1 cut(s) 141
HhaI GCGC 1 cut(s) 311
Hin1II CATG 2 cut(s) 66, 131
Hin6I GCGC 1 cut(s) 309
HinP1I GCGC 1 cut(s) 309
HinfI GANTC 2 cut(s) 206, 371
HpaII CCGG 1 cut(s) 141
HphI GGTGA 3 cut(s) 162, 218, 373
Hpy99I CGWCG 1 cut(s) 404
HpyAV CCTTC 2 cut(s) 59, 184
HpyCH4III ACNGT 2 cut(s) 181, 344
HpyCH4IV ACGT 1 cut(s) 335
HpyCH4V TGCA 3 cut(s) 4, 184, 351
HpySE526I ACGT 1 cut(s) 335
Hsp92II CATG 2 cut(s) 66, 131
HspAI GCGC 1 cut(s) 309
LpnPI CCDG 2 cut(s) 8, 154
Lsp1109I GCAGC 1 cut(s) 196
LweI GCATC 2 cut(s) 13, 303
MaeI CTAG 1 cut(s) 423
MaeII ACGT 1 cut(s) 335
MaeIII GTNAC 2 cut(s) 73, 344
MboII GAAGA 6 cut(s) 103, 109, 131, 308, 314, 380
MluCI AATT 2 cut(s) 216, 428
MmeI TCCRAC 1 cut(s) 229
MnlI CCTC 1 cut(s) 286
Mph1103I ATGCAT 1 cut(s) 6
MroXI GAANNNNTTC 1 cut(s) 219
MseI TTAA 1 cut(s) 318
MspA1I CMGCKG 1 cut(s) 261
MspCI CTTAAG 1 cut(s) 317
MspI CCGG 1 cut(s) 141
MvnI CGCG 1 cut(s) 311
NlaIII CATG 2 cut(s) 66, 131
NmuCI GTSAC 1 cut(s) 344
NsiI ATGCAT 1 cut(s) 6
PdmI GAANNNNTTC 1 cut(s) 219
PfeI GAWTC 2 cut(s) 206, 371
PkrI GCNGC 1 cut(s) 186
Ppu21I YACGTR 1 cut(s) 336
PspPI GGNCC 1 cut(s) 193
RsaI GTAC 1 cut(s) 338
RsaNI GTAC 1 cut(s) 337
SaqAI TTAA 1 cut(s) 318
SatI GCNGC 1 cut(s) 185
Sau96I GGNCC 1 cut(s) 193
SetI ASST 5 cut(s) 80, 247, 338, 363, 424
SfaNI GCATC 2 cut(s) 13, 303
SmlI CTYRAG 1 cut(s) 317
SmoI CTYRAG 1 cut(s) 317
Sse9I AATT 2 cut(s) 216, 428
SsiI CCGC 2 cut(s) 261, 380
SspMI CTAG 1 cut(s) 423
TaaI ACNGT 2 cut(s) 181, 344
TaiI ACGT 1 cut(s) 338
TaqI TCGA 2 cut(s) 67, 402
TasI AATT 2 cut(s) 216, 428
TfiI GAWTC 2 cut(s) 206, 371
Tru1I TTAA 1 cut(s) 318
Tru9I TTAA 1 cut(s) 318
TscAI CASTG 1 cut(s) 186
TseFI GTSAC 1 cut(s) 344
TseI GCWGC 1 cut(s) 184
Tsp45I GTSAC 1 cut(s) 344
TspDTI ATGAA 4 cut(s) 110, 132, 212, 228
TspRI CASTG 1 cut(s) 186
Vha464I CTTAAG 1 cut(s) 317
XapI RAATTY 1 cut(s) 216
XmnI GAANNNNTTC 1 cut(s) 219
XspI CTAG 1 cut(s) 423
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.