Rh2AG068500

Transmembrane proteins 14C

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0002220
Physical Location & Seq
Forward (+)
5683713 .. 5684261
549 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG068500.1

Sequence Viewer

Length: 549 bp
ATGACATCGTTTTTGGCCCCTGCTCCTCAACCCTCGCATCAGGGATTAAATCCTGGTGAGTTTGGATTTATTATACTCACAGTATACATACTAATTGTTCTCTGCGTCAGAAAACCAGAGTACGTCCTCTATCCTTGTTTAAAGCTTCTGTGTGTCTTCATACTCGGGCCATTTGTTGTGTGCGCCTTCATACTACTCGGGCCAATTTTTGTGTGCTTCAAAATTATACAGACCATCATTGTAGATACGAGACACCAAACAGGTCAAGCCCACCTTGATATCGAATCTGGAGGCCACCAGTTACCAGCCGCTCCACCGCAGCAACCTGCTGGTAACAATGCTGGACAGCCACGACGGCCGAAGGATTGGAGTGGATACTATGAAATTTTTAGTCATCTGAAAGTTGGTGAGTGTAGTATTTGCCTGGGAGAGTTTGAGGAACCAGATGAGTGTGCGAGACTTCGTAAGTGTAAGCATACGTATCACCGGGAATGCATTGATCTGTGGCTACCTAAATCGTGTCCGATTTGTCGTCAAAACGGAGTATAA

Protein Analysis

182

Amino Acids

20.41

Weight (kDa)

7.52

Isoelectric Point (pI)

50.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 137 - 178 4.4e-11 Ring finger domain
zf-RING_11 PF17123 137 - 166 3.3e-09 RING-like zinc finger
zf-RING_5 PF14634 137 - 179 6.6e-07 zinc-RING finger domain
zf-C3HC4_2 PF13923 137 - 177 5.4e-06 Zinc finger, C3HC4 type (RING finger)
zf-RING_UBOX PF13445 138 - 169 1e-06 RING-type zinc-finger
zf-C3HC4 PF00097 138 - 177 6e-06 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 334
AccBSI CCGCTC 1 cut(s) 311
AccI GTMKAC 1 cut(s) 84
AciI CCGC 2 cut(s) 309, 317
AcoI YGGCCR 1 cut(s) 356
AcsI RAATTY 1 cut(s) 384
AfaI GTAC 1 cut(s) 122
AgsI TTSAA 1 cut(s) 220
AjnI CCWGG 2 cut(s) 52, 423
AluBI AGCT 1 cut(s) 145
AluI AGCT 1 cut(s) 145
Alw26I GTCTC 2 cut(s) 244, 451
Ama87I CYCGRG 2 cut(s) 164, 197
AoxI GGCC 5 cut(s) 15, 167, 200, 292, 356
ApeKI GCWGC 1 cut(s) 319
ApoI RAATTY 1 cut(s) 384
ArsI GACNNNNNNTTYG 1 cut(s) 27
AspLEI GCGC 1 cut(s) 185
AspS9I GGNCC 3 cut(s) 16, 167, 200
AsuC2I CCSGG 1 cut(s) 488
AsuHPI GGTGA 3 cut(s) 68, 419, 476
AvaI CYCGRG 2 cut(s) 164, 197
BbsI GAAGAC 1 cut(s) 148
BbvI GCAGC 1 cut(s) 331
BccI CCATC 1 cut(s) 242
BceAI ACGGC 1 cut(s) 371
BciT130I CCWGG 2 cut(s) 54, 425
BciVI GTATCC 1 cut(s) 368
BcnI CCSGG 1 cut(s) 488
BcoDI GTCTC 2 cut(s) 244, 451
BfuAI ACCTGC 1 cut(s) 334
BfuI GTATCC 1 cut(s) 368
BglI GCCNNNNNGGC 1 cut(s) 355
BisI GCNGC 2 cut(s) 309, 320
BlsI GCNGC 2 cut(s) 310, 321
Bme1390I CCNGG 3 cut(s) 54, 425, 488
BmeT110I CYCGRG 2 cut(s) 164, 197
BmgT120I GGNCC 3 cut(s) 16, 167, 200
BmiI GGNNCC 2 cut(s) 18, 441
BmrFI CCNGG 3 cut(s) 54, 425, 488
BmsI GCATC 1 cut(s) 46
BpiI GAAGAC 1 cut(s) 148
BpmI CTGGAG 1 cut(s) 309
BpuMI CCSGG 1 cut(s) 488
BsaAI YACGTR 1 cut(s) 480
BsaJI CCNNGG 1 cut(s) 424
Bse1I ACTGG 1 cut(s) 298
BseBI CCWGG 2 cut(s) 54, 425
BseDI CCNNGG 1 cut(s) 424
BseNI ACTGG 1 cut(s) 298
BseRI GAGGAG 1 cut(s) 15
BseX3I CGGCCG 1 cut(s) 356
BseXI GCAGC 1 cut(s) 331
Bsh1285I CGRYCG 1 cut(s) 359
BshFI GGCC 5 cut(s) 17, 169, 202, 294, 358
BsiEI CGRYCG 1 cut(s) 359
BsiHKCI CYCGRG 2 cut(s) 164, 197
BsiSI CCGG 1 cut(s) 487
BsmAI GTCTC 2 cut(s) 244, 451
BsmI GAATGC 1 cut(s) 497
BsnI GGCC 5 cut(s) 17, 169, 202, 294, 358
BsoBI CYCGRG 2 cut(s) 164, 197
Bsp143I GATC 1 cut(s) 499
BspACI CCGC 2 cut(s) 309, 317
BspANI GGCC 5 cut(s) 17, 169, 202, 294, 358
BspLI GGNNCC 2 cut(s) 18, 441
BspMI ACCTGC 1 cut(s) 334
BsrBI CCGCTC 1 cut(s) 311
BsrI ACTGG 1 cut(s) 298
BssECI CCNNGG 1 cut(s) 424
BssMI GATC 1 cut(s) 499
BssNAI GTATAC 1 cut(s) 85
Bst1107I GTATAC 1 cut(s) 85
Bst2UI CCWGG 2 cut(s) 54, 425
Bst4CI ACNGT 1 cut(s) 82
BstBAI YACGTR 1 cut(s) 480
BstHHI GCGC 1 cut(s) 185
BstKTI GATC 1 cut(s) 502
BstMAI GTCTC 2 cut(s) 244, 451
BstMBI GATC 1 cut(s) 499
BstMCI CGRYCG 1 cut(s) 359
BstMWI GCNNNNNNNGC 1 cut(s) 355
BstNI CCWGG 2 cut(s) 54, 425
BstSCI CCNGG 3 cut(s) 52, 423, 486
BstSNI TACGTA 1 cut(s) 480
BstV1I GCAGC 1 cut(s) 331
BstV2I GAAGAC 1 cut(s) 148
BstZ17I GTATAC 1 cut(s) 85
BstZI CGGCCG 1 cut(s) 356
BsuI GTATCC 1 cut(s) 368
BsuRI GGCC 5 cut(s) 17, 169, 202, 294, 358
BveI ACCTGC 1 cut(s) 334
CfoI GCGC 1 cut(s) 185
Cfr13I GGNCC 3 cut(s) 16, 167, 200
CseI GACGC 1 cut(s) 94
Csp6I GTAC 1 cut(s) 121
CviQI GTAC 1 cut(s) 121
DpnI GATC 1 cut(s) 501
DpnII GATC 1 cut(s) 499
DraI TTTAAA 1 cut(s) 141
EaeI YGGCCR 1 cut(s) 356
EagI CGGCCG 1 cut(s) 356
EclXI CGGCCG 1 cut(s) 356
Eco105I TACGTA 1 cut(s) 480
Eco32I GATATC 1 cut(s) 280
Eco52I CGGCCG 1 cut(s) 356
Eco88I CYCGRG 2 cut(s) 164, 197
EcoRII CCWGG 2 cut(s) 52, 423
EcoRV GATATC 1 cut(s) 280
EcoT22I ATGCAT 1 cut(s) 497
FaiI YATR 9 cut(s) 74, 85, 89, 161, 191, 227, 381, 477, 547
FalI AAGNNNNNCTT 2 cut(s) 258, 290
FblI GTMKAC 1 cut(s) 84
Fnu4HI GCNGC 2 cut(s) 309, 320
Fsp4HI GCNGC 2 cut(s) 309, 320
GlaI GCGC 1 cut(s) 184
GluI GCNGC 2 cut(s) 309, 320
GsuI CTGGAG 1 cut(s) 309
HaeIII GGCC 5 cut(s) 17, 169, 202, 294, 358
HapII CCGG 1 cut(s) 487
HgaI GACGC 1 cut(s) 94
HhaI GCGC 1 cut(s) 185
Hin6I GCGC 1 cut(s) 183
HinP1I GCGC 1 cut(s) 183
HindIII AAGCTT 1 cut(s) 143
HinfI GANTC 1 cut(s) 284
HpaII CCGG 1 cut(s) 487
HphI GGTGA 3 cut(s) 68, 419, 476
Hpy166II GTNNAC 1 cut(s) 85
Hpy188I TCNGA 3 cut(s) 110, 399, 525
Hpy188III TCNNGA 1 cut(s) 288
Hpy8I GTNNAC 1 cut(s) 85
Hpy99I CGWCG 1 cut(s) 357
HpyAV CCTTC 2 cut(s) 196, 355
HpyCH4III ACNGT 1 cut(s) 82
HpyCH4IV ACGT 2 cut(s) 123, 479
HpyCH4V TGCA 1 cut(s) 495
HpyF10VI GCNNNNNNNGC 1 cut(s) 355
HpySE526I ACGT 2 cut(s) 123, 479
HspAI GCGC 1 cut(s) 183
Kzo9I GATC 1 cut(s) 499
LmnI GCTCC 2 cut(s) 28, 316
Lsp1109I GCAGC 1 cut(s) 331
LweI GCATC 1 cut(s) 46
MaeII ACGT 2 cut(s) 123, 479
MaeIII GTNAC 2 cut(s) 300, 332
MalI GATC 1 cut(s) 501
MbiI CCGCTC 1 cut(s) 311
MboI GATC 1 cut(s) 499
MboII GAAGA 1 cut(s) 148
MluCI AATT 4 cut(s) 93, 204, 222, 384
MnlI CCTC 5 cut(s) 36, 43, 137, 284, 430
Mph1103I ATGCAT 1 cut(s) 497
MseI TTAA 2 cut(s) 47, 140
MspI CCGG 1 cut(s) 487
MspR9I CCNGG 3 cut(s) 54, 425, 488
Mva1269I GAATGC 1 cut(s) 497
MvaI CCWGG 2 cut(s) 54, 425
MwoI GCNNNNNNNGC 1 cut(s) 355
NciI CCSGG 1 cut(s) 488
NdeII GATC 1 cut(s) 499
NlaIV GGNNCC 2 cut(s) 18, 441
NsiI ATGCAT 1 cut(s) 497
PctI GAATGC 1 cut(s) 497
PfeI GAWTC 1 cut(s) 284
PkrI GCNGC 2 cut(s) 310, 321
Ppu21I YACGTR 1 cut(s) 480
Psp6I CCWGG 2 cut(s) 52, 423
PspGI CCWGG 2 cut(s) 52, 423
PspN4I GGNNCC 2 cut(s) 18, 441
PspPI GGNCC 3 cut(s) 16, 167, 200
RsaI GTAC 1 cut(s) 122
RsaNI GTAC 1 cut(s) 121
SaqAI TTAA 2 cut(s) 47, 140
SatI GCNGC 2 cut(s) 309, 320
Sau3AI GATC 1 cut(s) 499
Sau96I GGNCC 3 cut(s) 16, 167, 200
ScrFI CCNGG 3 cut(s) 54, 425, 488
SetI ASST 7 cut(s) 126, 147, 265, 276, 328, 482, 514
SfaNI GCATC 1 cut(s) 46
SnaBI TACGTA 1 cut(s) 480
Sse9I AATT 4 cut(s) 93, 204, 222, 384
SsiI CCGC 2 cut(s) 309, 317
StyD4I CCNGG 3 cut(s) 52, 423, 486
TaaI ACNGT 1 cut(s) 82
TaiI ACGT 2 cut(s) 126, 482
TaqI TCGA 1 cut(s) 282
TasI AATT 4 cut(s) 93, 204, 222, 384
TauI GCSGC 1 cut(s) 311
TfiI GAWTC 1 cut(s) 284
Tru1I TTAA 2 cut(s) 47, 140
Tru9I TTAA 2 cut(s) 47, 140
TseI GCWGC 1 cut(s) 319
TspDTI ATGAA 3 cut(s) 148, 178, 396
XapI RAATTY 1 cut(s) 384
XmiI GTMKAC 1 cut(s) 84
Zsp2I ATGCAT 1 cut(s) 497
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.