Rroxscaffold_2G00149500

Transmembrane proteins 14C

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0002220
Physical Location & Seq
Reverse (-)
87057724 .. 87060283
2560 bp
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UTR
Exon/CDS
Intron
Rroxscaffold_2G00149500.1

Sequence Viewer

Length: 678 bp
ATGAAACAGAGGAAGATATTGAATCCGGCCAGCTATGATCAGAAAGTTCAGAAACTCCACCATCACAGGAGCCGAGTAACAATGTCAATGTTGATGCACAAACAGCATAGCAGGATTGGTACCAATGCTATGAAACTTTCGACTATCGAATGGAAGTTGTTAATGGAACTTGCAGCTTTATTGGGGATCAGTGTAGTATTTGCCAGGAGAAGTTTGAGGAAGACGAAGAAGATGAGTGCAAAACCAGAGTACGTCCTCTATCCTTGTTTAAAGCTTCTGTGTGTCTTCATACTTGGGCCATTTGTTGTGTGCGCCTTCATACTACTCGGGCCAATTTTTGTGTGCTTCAAAATTATACAGACCATCATTGTAGATACGAGACACCAAACAGGTCAAGCTCACCTTGATATCGAATCTGGCGGCCACCAGTTACCAGCCGCTCCACCGCAGCAACCTGCTGGTAACAATGCTGGACAGCCACGACGGCCGAAGGATTGGAGTGGATACTATGAAATTTTTAGTCATCTGAAAGTTGGTGAGTGTAGTATTTGCCTGGGAGAGTTTGAGGAACCAGATGAGTGTGCGAGACTTCGTAAGTGTAAGCATACGTATCACCGGGAATGCATTGATCTGTGGCTACCTAAATCGTGTCCGATTTGTCGTCAAAACGGAGTATAA

Protein Analysis

225

Amino Acids

25.69

Weight (kDa)

9.49

Isoelectric Point (pI)

57.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 180 - 221 6.2e-11 Ring finger domain
zf-RING_11 PF17123 180 - 209 4.5e-09 RING-like zinc finger
zf-RING_5 PF14634 180 - 222 9.5e-07 zinc-RING finger domain
zf-C3HC4_2 PF13923 180 - 220 7.6e-06 Zinc finger, C3HC4 type (RING finger)
zf-RING_UBOX PF13445 181 - 212 1.5e-06 RING-type zinc-finger
zf-C3HC4 PF00097 181 - 220 8.6e-06 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 463
Acc65I GGTACC 1 cut(s) 119
AccB1I GGYRCC 1 cut(s) 119
AccBSI CCGCTC 1 cut(s) 440
AciI CCGC 3 cut(s) 420, 438, 446
AclWI GGATC 1 cut(s) 194
AcoI YGGCCR 3 cut(s) 27, 421, 485
AcsI RAATTY 1 cut(s) 513
AfaI GTAC 2 cut(s) 121, 251
AgsI TTSAA 2 cut(s) 22, 349
AjnI CCWGG 2 cut(s) 203, 552
AluBI AGCT 4 cut(s) 33, 176, 274, 398
AluI AGCT 4 cut(s) 33, 176, 274, 398
Alw26I GTCTC 2 cut(s) 373, 580
AlwI GGATC 1 cut(s) 194
Ama87I CYCGRG 1 cut(s) 326
AoxI GGCC 5 cut(s) 27, 296, 329, 421, 485
ApeKI GCWGC 2 cut(s) 173, 448
ApoI RAATTY 1 cut(s) 513
Asp718I GGTACC 1 cut(s) 119
AspLEI GCGC 1 cut(s) 314
AspS9I GGNCC 2 cut(s) 296, 329
AsuC2I CCSGG 1 cut(s) 617
AsuHPI GGTGA 3 cut(s) 392, 548, 605
AvaI CYCGRG 1 cut(s) 326
BanI GGYRCC 1 cut(s) 119
BbsI GAAGAC 2 cut(s) 227, 277
BbvI GCAGC 2 cut(s) 185, 460
BccI CCATC 2 cut(s) 69, 371
BceAI ACGGC 1 cut(s) 500
BciT130I CCWGG 2 cut(s) 205, 554
BciVI GTATCC 1 cut(s) 497
BclI TGATCA 1 cut(s) 37
BcnI CCSGG 1 cut(s) 617
BcoDI GTCTC 2 cut(s) 373, 580
BfuAI ACCTGC 1 cut(s) 463
BfuI GTATCC 1 cut(s) 497
BglI GCCNNNNNGGC 1 cut(s) 484
BisI GCNGC 4 cut(s) 174, 421, 438, 449
BlsI GCNGC 4 cut(s) 175, 422, 439, 450
Bme1390I CCNGG 3 cut(s) 205, 554, 617
BmeT110I CYCGRG 1 cut(s) 326
BmgT120I GGNCC 2 cut(s) 296, 329
BmiI GGNNCC 3 cut(s) 71, 121, 570
BmrFI CCNGG 3 cut(s) 205, 554, 617
BmsI GCATC 1 cut(s) 84
BpiI GAAGAC 2 cut(s) 227, 277
BpuMI CCSGG 1 cut(s) 617
BsaAI YACGTR 1 cut(s) 609
BsaJI CCNNGG 1 cut(s) 553
Bse1I ACTGG 1 cut(s) 427
BseBI CCWGG 2 cut(s) 205, 554
BseDI CCNNGG 1 cut(s) 553
BseNI ACTGG 1 cut(s) 427
BseX3I CGGCCG 1 cut(s) 485
BseXI GCAGC 2 cut(s) 185, 460
Bsh1285I CGRYCG 1 cut(s) 488
BshFI GGCC 5 cut(s) 29, 298, 331, 423, 487
BshNI GGYRCC 1 cut(s) 119
BsiEI CGRYCG 1 cut(s) 488
BsiHKCI CYCGRG 1 cut(s) 326
BsiSI CCGG 2 cut(s) 26, 616
BsmAI GTCTC 2 cut(s) 373, 580
BsmI GAATGC 1 cut(s) 626
BsnI GGCC 5 cut(s) 29, 298, 331, 423, 487
BsoBI CYCGRG 1 cut(s) 326
Bsp143I GATC 3 cut(s) 37, 186, 628
BspACI CCGC 3 cut(s) 420, 438, 446
BspANI GGCC 5 cut(s) 29, 298, 331, 423, 487
BspLI GGNNCC 3 cut(s) 71, 121, 570
BspMI ACCTGC 1 cut(s) 463
BspPI GGATC 1 cut(s) 194
BspT107I GGYRCC 1 cut(s) 119
BsrBI CCGCTC 1 cut(s) 440
BsrI ACTGG 1 cut(s) 427
BssECI CCNNGG 1 cut(s) 553
BssMI GATC 3 cut(s) 37, 186, 628
Bst2UI CCWGG 2 cut(s) 205, 554
BstBAI YACGTR 1 cut(s) 609
BstC8I GCNNGC 1 cut(s) 31
BstHHI GCGC 1 cut(s) 314
BstKTI GATC 3 cut(s) 40, 189, 631
BstMAI GTCTC 2 cut(s) 373, 580
BstMBI GATC 3 cut(s) 37, 186, 628
BstMCI CGRYCG 1 cut(s) 488
BstMWI GCNNNNNNNGC 2 cut(s) 103, 484
BstNI CCWGG 2 cut(s) 205, 554
BstSCI CCNGG 3 cut(s) 203, 552, 615
BstSNI TACGTA 1 cut(s) 609
BstV1I GCAGC 2 cut(s) 185, 460
BstV2I GAAGAC 2 cut(s) 227, 277
BstZI CGGCCG 1 cut(s) 485
BsuI GTATCC 1 cut(s) 497
BsuRI GGCC 5 cut(s) 29, 298, 331, 423, 487
BtsIMutI CAGTG 1 cut(s) 196
BveI ACCTGC 1 cut(s) 463
Cac8I GCNNGC 1 cut(s) 31
CfoI GCGC 1 cut(s) 314
Cfr13I GGNCC 2 cut(s) 296, 329
Csp6I GTAC 2 cut(s) 120, 250
CviQI GTAC 2 cut(s) 120, 250
DpnI GATC 3 cut(s) 39, 188, 630
DpnII GATC 3 cut(s) 37, 186, 628
DraI TTTAAA 1 cut(s) 270
EaeI YGGCCR 3 cut(s) 27, 421, 485
EagI CGGCCG 1 cut(s) 485
EclXI CGGCCG 1 cut(s) 485
Eco105I TACGTA 1 cut(s) 609
Eco32I GATATC 1 cut(s) 409
Eco52I CGGCCG 1 cut(s) 485
Eco88I CYCGRG 1 cut(s) 326
EcoRII CCWGG 2 cut(s) 203, 552
EcoRV GATATC 1 cut(s) 409
EcoT22I ATGCAT 1 cut(s) 626
FaiI YATR 9 cut(s) 36, 108, 131, 290, 320, 356, 510, 606, 676
FalI AAGNNNNNCTT 2 cut(s) 387, 419
FbaI TGATCA 1 cut(s) 37
Fnu4HI GCNGC 4 cut(s) 174, 421, 438, 449
Fsp4HI GCNGC 4 cut(s) 174, 421, 438, 449
GlaI GCGC 1 cut(s) 313
GluI GCNGC 4 cut(s) 174, 421, 438, 449
HaeIII GGCC 5 cut(s) 29, 298, 331, 423, 487
HapII CCGG 2 cut(s) 26, 616
HhaI GCGC 1 cut(s) 314
Hin6I GCGC 1 cut(s) 312
HinP1I GCGC 1 cut(s) 312
HindIII AAGCTT 1 cut(s) 272
HinfI GANTC 2 cut(s) 22, 413
HpaII CCGG 2 cut(s) 26, 616
HphI GGTGA 3 cut(s) 392, 548, 605
Hpy188I TCNGA 4 cut(s) 42, 51, 528, 654
Hpy99I CGWCG 1 cut(s) 486
HpyAV CCTTC 2 cut(s) 325, 484
HpyCH4IV ACGT 2 cut(s) 252, 608
HpyCH4V TGCA 4 cut(s) 97, 173, 239, 624
HpyF10VI GCNNNNNNNGC 2 cut(s) 103, 484
HpySE526I ACGT 2 cut(s) 252, 608
HspAI GCGC 1 cut(s) 312
KpnI GGTACC 1 cut(s) 123
Ksp22I TGATCA 1 cut(s) 37
Kzo9I GATC 3 cut(s) 37, 186, 628
LmnI GCTCC 2 cut(s) 69, 445
Lsp1109I GCAGC 2 cut(s) 185, 460
LweI GCATC 1 cut(s) 84
MaeII ACGT 2 cut(s) 252, 608
MaeIII GTNAC 3 cut(s) 76, 429, 461
MalI GATC 3 cut(s) 39, 188, 630
MbiI CCGCTC 1 cut(s) 440
MboI GATC 3 cut(s) 37, 186, 628
MboII GAAGA 5 cut(s) 25, 232, 238, 241, 277
MluCI AATT 3 cut(s) 333, 351, 513
MnlI CCTC 4 cut(s) 3, 210, 266, 559
Mph1103I ATGCAT 1 cut(s) 626
MseI TTAA 2 cut(s) 161, 269
MspI CCGG 2 cut(s) 26, 616
MspR9I CCNGG 3 cut(s) 205, 554, 617
Mva1269I GAATGC 1 cut(s) 626
MvaI CCWGG 2 cut(s) 205, 554
MwoI GCNNNNNNNGC 2 cut(s) 103, 484
NciI CCSGG 1 cut(s) 617
NdeII GATC 3 cut(s) 37, 186, 628
NlaIV GGNNCC 3 cut(s) 71, 121, 570
NmeAIII GCCGAG 1 cut(s) 98
NsiI ATGCAT 1 cut(s) 626
PctI GAATGC 1 cut(s) 626
PfeI GAWTC 2 cut(s) 22, 413
PkrI GCNGC 4 cut(s) 175, 422, 439, 450
Ppu21I YACGTR 1 cut(s) 609
Psp6I CCWGG 2 cut(s) 203, 552
PspGI CCWGG 2 cut(s) 203, 552
PspN4I GGNNCC 3 cut(s) 71, 121, 570
PspPI GGNCC 2 cut(s) 296, 329
RsaI GTAC 2 cut(s) 121, 251
RsaNI GTAC 2 cut(s) 120, 250
SaqAI TTAA 2 cut(s) 161, 269
SatI GCNGC 4 cut(s) 174, 421, 438, 449
Sau3AI GATC 3 cut(s) 37, 186, 628
Sau96I GGNCC 2 cut(s) 296, 329
ScrFI CCNGG 3 cut(s) 205, 554, 617
SfaNI GCATC 1 cut(s) 84
SnaBI TACGTA 1 cut(s) 609
Sse9I AATT 3 cut(s) 333, 351, 513
SsiI CCGC 3 cut(s) 420, 438, 446
StyD4I CCNGG 3 cut(s) 203, 552, 615
TaiI ACGT 2 cut(s) 255, 611
TaqI TCGA 3 cut(s) 140, 147, 411
TasI AATT 3 cut(s) 333, 351, 513
TauI GCSGC 2 cut(s) 423, 440
TfiI GAWTC 2 cut(s) 22, 413
Tru1I TTAA 2 cut(s) 161, 269
Tru9I TTAA 2 cut(s) 161, 269
TscAI CASTG 1 cut(s) 196
TseI GCWGC 2 cut(s) 173, 448
TspDTI ATGAA 5 cut(s) 17, 146, 277, 307, 525
TspRI CASTG 1 cut(s) 196
XapI RAATTY 1 cut(s) 513
Zsp2I ATGCAT 1 cut(s) 626
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.