FvH4_1g02260

F-box kelch-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0001674
Physical Location & Seq
Forward (+)
1216173 .. 1217809
1637 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g02260.t1

Sequence Viewer

Length: 1101 bp
ATGGTGGCTAATGCAACATTACCCGAAGAGCTGATACTCCAAATCGTGTCCAGGGCGACGGTGAAGACGGTGGGGCGATGCAGATGCGTCTCGACAGTGTGGCGCTCTCTCTTCTCTGATCCCCAATTCGCGAAATCGCACTTCCAGGTATCCCCTAAGCATCAAGCCCTAATATTTCTACGCGACGAAAAACTCAAAAGAGTGGATTTCGATTTGAGCAATACGGCGTCGTTTGAGAAAGAGTCTCTGGTTGTCCGAGATCTGAAGGCCCCTTTCGGTCACACTTGGCTCCGGTTTTTACGGTCCTGCAATGGGCTGGTGGCTTTAGCTGACTTCGGTCAGTTTCTAGGGAAAGAAGAAAGCTTGTTTATATGGAATCCGTCCACTGGGTTCTTGAAGAAATTACCATCCCGGCGATCTAATGAAAAATTCATATTTGGGTTTGGGTGCGCGTCCAGCAGCGATGAATACAAATTATTGGTGGGTGCGAAGATCTTTTCATTCAAGACTAACTCATGGAAGCCCATTGAAATGCCCCCCTCCTGCCGCCGCTTTGATCGGGGATATGGTGTGCTTTGCAGGGATGCATTGTATTGGCGGCCACTCTATCCGGTTCAAGGACAAGATTTGTATGCTTTCAATTTGGAAAAGGAGGAGTTTTCGATAATGAAGCTGCCTGAAGCTTTTCAAACAGGACGAGCCAATAGTTACAGTGTCATACGCGATCATCGTGTCACTTGTGATGGGTGCTTGTTTGTAATGGACGAGACTCCGGTGTGGGATGGTGGTTGTGCGGTGGAGTATCACCGTGAGTTTTGGGTCATGAGAGAGTATGGCAACAGTGATTCCTGGACTAGATCATTTGGCGTAAAGATCCCCTGCCCCAAACAGTCGGGGCTGAGGAGTTCGCCTCTAAACTTTGGAGCAATTTCGGTTATGGAAACTAGTGCATTTGTGAGGAATGAAGCAAAAGAAGAAGAACAGAAACTCATGTCTACCACTTATTATCGCTATGAAGAAGAGAACCCCCACATGTATATGATTGAGAGTGGCTCGTGTCAGAAGGTTGTATACGAAGAGAGTCTGTATTGTCTTGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like FBA_1 FBA_3

Protein Analysis

367

Amino Acids

42.13

Weight (kDa)

8.18

Isoelectric Point (pI)

50.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box-like PF12937 6 - 48 1.3e-07 F-box-like
F-box PF00646 7 - 43 1.7e-06 F-box domain
FBA_1 PF07734 58 - 360 4.5e-15 F-box associated beta propeller domain
FBA_3 PF08268 61 - 288 7.9e-09 F-box associated beta propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 995, 1071
AccII CGCG 4 cut(s) 131, 183, 452, 723
AciI CCGC 4 cut(s) 547, 550, 598, 794
AclWI GGATC 2 cut(s) 113, 868
AcoI YGGCCR 1 cut(s) 599
AcsI RAATTY 1 cut(s) 428
AcuI CTGAAG 2 cut(s) 284, 699
AcyI GRCGYC 1 cut(s) 227
AfiI CCNNNNNNNGG 3 cut(s) 312, 386, 617
AflIII ACRYGT 1 cut(s) 1032
AgsI TTSAA 6 cut(s) 397, 505, 530, 617, 640, 689
AhlI ACTAGT 1 cut(s) 944
AjnI CCWGG 3 cut(s) 50, 144, 848
AluBI AGCT 5 cut(s) 31, 329, 363, 673, 683
AluI AGCT 5 cut(s) 31, 329, 363, 673, 683
Alw26I GTCTC 3 cut(s) 94, 249, 761
AlwI GGATC 2 cut(s) 113, 868
AoxI GGCC 2 cut(s) 267, 599
ApeKI GCWGC 2 cut(s) 459, 673
ApoI RAATTY 1 cut(s) 428
Asp700I GAANNNNTTC 1 cut(s) 684
AspLEI GCGC 2 cut(s) 105, 452
AspS9I GGNCC 2 cut(s) 268, 303
AsuC2I CCSGG 1 cut(s) 412
AsuHPI GGTGA 2 cut(s) 73, 797
AvaII GGWCC 1 cut(s) 303
BarI GAAGNNNNNNTAC 4 cut(s) 18, 50, 1055, 1087
BauI CACGAG 1 cut(s) 1054
BbsI GAAGAC 1 cut(s) 71
BbvCI CCTCAGC 1 cut(s) 899
BbvI GCAGC 2 cut(s) 471, 660
BccI CCATC 3 cut(s) 415, 737, 776
BceAI ACGGC 1 cut(s) 240
BcgI CGANNNNNNTGC 2 cut(s) 66, 100
BciT130I CCWGG 3 cut(s) 52, 146, 850
BciVI GTATCC 1 cut(s) 160
BcnI CCSGG 1 cut(s) 412
BcoDI GTCTC 3 cut(s) 94, 249, 761
BcuI ACTAGT 1 cut(s) 944
BfaI CTAG 3 cut(s) 347, 855, 945
BfoI RGCGCY 1 cut(s) 106
BfuI GTATCC 1 cut(s) 160
BglII AGATCT 2 cut(s) 259, 492
BisI GCNGC 5 cut(s) 460, 547, 550, 599, 674
BlsI GCNGC 5 cut(s) 461, 548, 551, 600, 675
Bme1390I CCNGG 4 cut(s) 52, 146, 412, 850
Bme18I GGWCC 1 cut(s) 303
BmgT120I GGNCC 2 cut(s) 268, 303
BmiI GGNNCC 2 cut(s) 270, 290
BmrFI CCNGG 4 cut(s) 52, 146, 412, 850
BmrI ACTGGG 1 cut(s) 396
BmsI GCATC 4 cut(s) 68, 74, 169, 574
BmuI ACTGGG 1 cut(s) 396
BoxI GACNNNNGTC 1 cut(s) 336
BpiI GAAGAC 1 cut(s) 71
BplI GAGNNNNNCTC 4 cut(s) 895, 927, 1037, 1069
Bpu10I CCTNAGC 2 cut(s) 156, 899
BpuMI CCSGG 1 cut(s) 412
BsaHI GRCGYC 1 cut(s) 227
BsaJI CCNNGG 1 cut(s) 51
BsaWI WCCGGW 3 cut(s) 291, 610, 772
Bsc4I CCNNNNNNNGG 3 cut(s) 312, 386, 617
Bse1I ACTGG 1 cut(s) 391
Bse3DI GCAATG 1 cut(s) 316
BseBI CCWGG 3 cut(s) 52, 146, 850
BseDI CCNNGG 1 cut(s) 51
BseGI GGATG 3 cut(s) 407, 589, 787
BseLI CCNNNNNNNGG 3 cut(s) 312, 386, 617
BseMI GCAATG 1 cut(s) 316
BseMII CTCAG 1 cut(s) 890
BseNI ACTGG 1 cut(s) 391
BseRI GAGGAG 2 cut(s) 668, 916
BseXI GCAGC 2 cut(s) 471, 660
Bsh1236I CGCG 4 cut(s) 131, 183, 452, 723
BshFI GGCC 2 cut(s) 269, 601
BsiSI CCGG 4 cut(s) 292, 412, 611, 773
BslI CCNNNNNNNGG 3 cut(s) 312, 386, 617
BsmAI GTCTC 3 cut(s) 94, 249, 761
BsmBI CGTCTC 1 cut(s) 94
BsnI GGCC 2 cut(s) 269, 601
Bsp143I GATC 8 cut(s) 118, 259, 416, 492, 556, 724, 857, 873
Bsp68I TCGCGA 1 cut(s) 131
BspACI CCGC 4 cut(s) 547, 550, 598, 794
BspANI GGCC 2 cut(s) 269, 601
BspCNI CTCAG 1 cut(s) 891
BspFNI CGCG 4 cut(s) 131, 183, 452, 723
BspHI TCATGA 1 cut(s) 822
BspLI GGNNCC 2 cut(s) 270, 290
BspPI GGATC 2 cut(s) 113, 868
BspQI GCTCTTC 1 cut(s) 21
BsrDI GCAATG 1 cut(s) 316
BsrI ACTGG 1 cut(s) 391
BssECI CCNNGG 1 cut(s) 51
BssMI GATC 8 cut(s) 118, 259, 416, 492, 556, 724, 857, 873
BssNAI GTATAC 1 cut(s) 1072
BssNI GRCGYC 1 cut(s) 227
BssSI CACGAG 1 cut(s) 1054
Bst1107I GTATAC 1 cut(s) 1072
Bst2BI CACGAG 1 cut(s) 1054
Bst2UI CCWGG 3 cut(s) 52, 146, 850
Bst4CI ACNGT 8 cut(s) 61, 70, 97, 303, 713, 809, 842, 891
Bst6I CTCTTC 4 cut(s) 21, 116, 1014, 1071
BstACI GRCGYC 1 cut(s) 227
BstDEI CTNAG 3 cut(s) 156, 899, 1098
BstF5I GGATG 3 cut(s) 407, 589, 787
BstFNI CGCG 4 cut(s) 131, 183, 452, 723
BstH2I RGCGCY 1 cut(s) 106
BstHHI GCGC 2 cut(s) 105, 452
BstKTI GATC 8 cut(s) 121, 262, 419, 495, 559, 727, 860, 876
BstMAI GTCTC 3 cut(s) 94, 249, 761
BstMBI GATC 8 cut(s) 118, 259, 416, 492, 556, 724, 857, 873
BstMWI GCNNNNNNNGC 1 cut(s) 456
BstNI CCWGG 3 cut(s) 52, 146, 850
BstNSI RCATGY 1 cut(s) 1036
BstPAI GACNNNNGTC 1 cut(s) 336
BstSCI CCNGG 4 cut(s) 50, 144, 410, 848
BstUI CGCG 4 cut(s) 131, 183, 452, 723
BstV1I GCAGC 2 cut(s) 471, 660
BstV2I GAAGAC 1 cut(s) 71
BstX2I RGATCY 3 cut(s) 259, 492, 873
BstYI RGATCY 3 cut(s) 259, 492, 873
BstZ17I GTATAC 1 cut(s) 1072
BsuI GTATCC 1 cut(s) 160
BsuRI GGCC 2 cut(s) 269, 601
BtgZI GCGATG 2 cut(s) 91, 477
BtsCI GGATG 3 cut(s) 407, 589, 787
BtsIMutI CAGTG 4 cut(s) 102, 384, 718, 847
BtuMI TCGCGA 1 cut(s) 131
CciI TCATGA 1 cut(s) 822
CfoI GCGC 2 cut(s) 105, 452
Cfr13I GGNCC 2 cut(s) 268, 303
CseI GACGC 3 cut(s) 76, 216, 441
CviAII CATG 4 cut(s) 516, 823, 991, 1033
DdeI CTNAG 3 cut(s) 156, 899, 1098
DpnI GATC 8 cut(s) 120, 261, 418, 494, 558, 726, 859, 875
DpnII GATC 8 cut(s) 118, 259, 416, 492, 556, 724, 857, 873
EaeI YGGCCR 1 cut(s) 599
Eam1104I CTCTTC 4 cut(s) 21, 116, 1014, 1071
EarI CTCTTC 4 cut(s) 21, 116, 1014, 1071
Eco47I GGWCC 1 cut(s) 303
Eco57I CTGAAG 2 cut(s) 284, 699
EcoO109I RGGNCCY 1 cut(s) 268
EcoRII CCWGG 3 cut(s) 50, 144, 848
EcoT22I ATGCAT 1 cut(s) 589
Esp3I CGTCTC 1 cut(s) 94
FaeI CATG 4 cut(s) 519, 826, 994, 1036
FatI CATG 4 cut(s) 515, 822, 990, 1032
FblI GTMKAC 2 cut(s) 995, 1071
Fnu4HI GCNGC 5 cut(s) 460, 547, 550, 599, 674
FokI GGATG 3 cut(s) 394, 596, 794
Fsp4HI GCNGC 5 cut(s) 460, 547, 550, 599, 674
FspBI CTAG 3 cut(s) 347, 855, 945
GlaI GCGC 2 cut(s) 104, 451
GluI GCNGC 5 cut(s) 460, 547, 550, 599, 674
HaeII RGCGCY 1 cut(s) 106
HaeIII GGCC 2 cut(s) 269, 601
HapII CCGG 4 cut(s) 292, 412, 611, 773
HgaI GACGC 3 cut(s) 76, 216, 441
HhaI GCGC 2 cut(s) 105, 452
Hin1I GRCGYC 1 cut(s) 227
Hin1II CATG 4 cut(s) 519, 826, 994, 1036
Hin6I GCGC 2 cut(s) 103, 450
HinP1I GCGC 2 cut(s) 103, 450
HindIII AAGCTT 2 cut(s) 361, 681
HinfI GANTC 5 cut(s) 242, 376, 769, 845, 1081
HpaII CCGG 4 cut(s) 292, 412, 611, 773
HphI GGTGA 2 cut(s) 73, 797
Hpy166II GTNNAC 3 cut(s) 384, 996, 1072
Hpy188I TCNGA 4 cut(s) 118, 257, 264, 1062
Hpy188III TCNNGA 5 cut(s) 91, 130, 394, 505, 823
Hpy8I GTNNAC 3 cut(s) 384, 996, 1072
Hpy99I CGWCG 3 cut(s) 61, 188, 232
HpyAV CCTTC 2 cut(s) 259, 1057
HpyCH4III ACNGT 8 cut(s) 61, 70, 97, 303, 713, 809, 842, 891
HpyCH4V TGCA 6 cut(s) 14, 81, 309, 579, 587, 950
HpyF10VI GCNNNNNNNGC 1 cut(s) 456
HpyF3I CTNAG 3 cut(s) 156, 899, 1098
Hsp92I GRCGYC 1 cut(s) 227
Hsp92II CATG 4 cut(s) 519, 826, 994, 1036
HspAI GCGC 2 cut(s) 103, 450
Kzo9I GATC 8 cut(s) 118, 259, 416, 492, 556, 724, 857, 873
LguI GCTCTTC 1 cut(s) 21
LmnI GCTCC 2 cut(s) 294, 923
Lsp1109I GCAGC 2 cut(s) 471, 660
LweI GCATC 4 cut(s) 68, 74, 169, 574
MaeI CTAG 3 cut(s) 347, 855, 945
MaeIII GTNAC 3 cut(s) 278, 707, 733
MalI GATC 8 cut(s) 120, 261, 418, 494, 558, 726, 859, 875
MboI GATC 8 cut(s) 118, 259, 416, 492, 556, 724, 857, 873
MflI RGATCY 3 cut(s) 259, 492, 873
MluCI AATT 6 cut(s) 125, 401, 428, 473, 640, 927
MlyI GAGTC 3 cut(s) 251, 763, 1090
MnlI CCTC 5 cut(s) 550, 646, 894, 921, 951
Mph1103I ATGCAT 1 cut(s) 589
MroXI GAANNNNTTC 1 cut(s) 684
MslI CAYNNNNRTG 2 cut(s) 530, 1037
MspI CCGG 4 cut(s) 292, 412, 611, 773
MspR9I CCNGG 4 cut(s) 52, 146, 412, 850
MvaI CCWGG 3 cut(s) 52, 146, 850
MvnI CGCG 4 cut(s) 131, 183, 452, 723
MwoI GCNNNNNNNGC 1 cut(s) 456
NciI CCSGG 1 cut(s) 412
NdeII GATC 8 cut(s) 118, 259, 416, 492, 556, 724, 857, 873
NlaIII CATG 4 cut(s) 519, 826, 994, 1036
NlaIV GGNNCC 2 cut(s) 270, 290
NmuCI GTSAC 2 cut(s) 278, 733
NruI TCGCGA 1 cut(s) 131
NsiI ATGCAT 1 cut(s) 589
NspI RCATGY 1 cut(s) 1036
PagI TCATGA 1 cut(s) 822
PciI ACATGT 1 cut(s) 1032
PciSI GCTCTTC 1 cut(s) 21
PcsI WCGNNNNNNNCGW 1 cut(s) 727
PdmI GAANNNNTTC 1 cut(s) 684
PfeI GAWTC 2 cut(s) 376, 845
PfoI TCCNGGA 1 cut(s) 848
PkrI GCNGC 5 cut(s) 461, 548, 551, 600, 675
PleI GAGTC 3 cut(s) 250, 763, 1089
PpsI GAGTC 3 cut(s) 250, 763, 1089
PscI ACATGT 1 cut(s) 1032
PshAI GACNNNNGTC 1 cut(s) 336
Psp6I CCWGG 3 cut(s) 50, 144, 848
PspGI CCWGG 3 cut(s) 50, 144, 848
PspN4I GGNNCC 2 cut(s) 270, 290
PspPI GGNCC 2 cut(s) 268, 303
PsuI RGATCY 3 cut(s) 259, 492, 873
RruI TCGCGA 1 cut(s) 131
RseI CAYNNNNRTG 2 cut(s) 530, 1037
SapI GCTCTTC 1 cut(s) 21
SatI GCNGC 5 cut(s) 460, 547, 550, 599, 674
Sau3AI GATC 8 cut(s) 118, 259, 416, 492, 556, 724, 857, 873
Sau96I GGNCC 2 cut(s) 268, 303
SchI GAGTC 3 cut(s) 251, 763, 1090
ScrFI CCNGG 4 cut(s) 52, 146, 412, 850
SetI ASST 7 cut(s) 33, 150, 331, 365, 675, 685, 1068
SfaNI GCATC 4 cut(s) 68, 74, 169, 574
SinI GGWCC 1 cut(s) 303
SmiMI CAYNNNNRTG 2 cut(s) 530, 1037
SpeI ACTAGT 1 cut(s) 944
Sse9I AATT 6 cut(s) 125, 401, 428, 473, 640, 927
SsiI CCGC 4 cut(s) 547, 550, 598, 794
SspI AATATT 1 cut(s) 174
SspMI CTAG 3 cut(s) 347, 855, 945
StyD4I CCNGG 4 cut(s) 50, 144, 410, 848
TaaI ACNGT 8 cut(s) 61, 70, 97, 303, 713, 809, 842, 891
TaqI TCGA 3 cut(s) 92, 210, 662
TaqII GACCGA 2 cut(s) 266, 326
TasI AATT 6 cut(s) 125, 401, 428, 473, 640, 927
TauI GCSGC 3 cut(s) 549, 552, 601
TfiI GAWTC 2 cut(s) 376, 845
TscAI CASTG 4 cut(s) 102, 391, 718, 847
TseFI GTSAC 2 cut(s) 278, 733
TseI GCWGC 2 cut(s) 459, 673
Tsp45I GTSAC 2 cut(s) 278, 733
TspDTI ATGAA 7 cut(s) 421, 438, 480, 489, 683, 978, 1029
TspGWI ACGGA 1 cut(s) 369
TspRI CASTG 4 cut(s) 102, 391, 718, 847
VpaK11BI GGWCC 1 cut(s) 303
XapI RAATTY 1 cut(s) 428
XceI RCATGY 1 cut(s) 1036
XmiI GTMKAC 2 cut(s) 995, 1071
XmnI GAANNNNTTC 1 cut(s) 684
XspI CTAG 3 cut(s) 347, 855, 945
Zsp2I ATGCAT 1 cut(s) 589
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.