FvH4_7g32350

F-box kelch-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0001674
Physical Location & Seq
Forward (+)
23269514 .. 23271036
1523 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g32350.t1

Sequence Viewer

Length: 1128 bp
ATGGCAGCTAATGCATTCTTACCCGAAGAGATCATTTTGGAAATCGTGGCCAGGCTACCGGTCAAATCAGTGGGGCGATGCAGATGCGTCTCTACACGGTGGCGCTCTCTATTCTCCGATCCCCACTTCGCGAAATCGCACTTCCAGTTAGGCCCCAAGCATCGAGCCTTAATAATTCAACACCTCATAAAACTCGAAACCCTAGCTTCTGATTTAAGTAACACATCTTTGTTCGAGAAGGAATCTCTGGTTCTCCGAAATCTGAATTCCCCTTTCTATCACAAGCACGTCCAGTTTTTAGGCTCATGCAATGGGCTGGTGGCTGTACGTGTCTTTCATGTGTTTCATCCATACTCCGAACAAGAAACAGAAAGCTTTCTTATATGGAACCCATCCACCGGGTTCTTCAAGAATTTTCCCGGGCGGGGTCATATGGATAATCATAAAGGGATCAAATGCGGGTTTGGGTATGTGTCCAAAACCGACGAATACAAACTTGTGATGGGTGATGCGATCTTGTCGTCCAAGACTGAATCATGGAAGCTGATGGAAATATTGCCTCCCGGCGCCGACGTCTGGATGATAGATGGTGTGTTTTTTCGTGATGCACTATATTGGCTAGAGTGTAGTCGACACCAGGAACAAGATCAGGCACGTCATATGTATGCTTTCGATTTAGAAAAGGAGGAGTTTTCGATAATGCAGCTGCCTAGTGCTCTTCAAACACTAGACATCAGAACGTGGAACCATGGTGTTACTCGTGAAGGGTGCTTGTATGCAATGGTCCTGAGAAAAGTGTACAGGGATTGTGTTGAATTTGACTATTATGTGGTTGAATTATGGGTCATGAGAGACTATGGCAACAATGATTATTGCTGGACTAGATCATTCGGCGTAAAGATTCCCAGTACTGCACGAAGACATTGGTATCTGTGGGTTGGATCAATTTCGGTTTCGGAGACTAGTGCATCAGTGATGACTAACTGTCCAGTAGGGAAACCAGCAAATTATAAACTCATGCCTACCAGTCATTATCGTAATGAAGAAGAGAACTCTGACATCTGGATGATTGAGAATAATGAACCAGACATGGTTGTATACGAAGAGAGTCTATATCGTCTTGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like FBA_1 FBA_3

Protein Analysis

376

Amino Acids

43.68

Weight (kDa)

6.21

Isoelectric Point (pI)

46.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 7 - 43 8.7e-11 F-box domain
F-box-like PF12937 7 - 46 3.2e-09 F-box-like
FBA_3 PF08268 85 - 290 1.1e-08 F-box associated beta propeller domain
FBA_1 PF07734 117 - 329 9.1e-16 F-box associated beta propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1011
AatII GACGTC 1 cut(s) 576
AccB1I GGYRCC 1 cut(s) 566
AccI GTMKAC 2 cut(s) 631, 1098
AccII CGCG 1 cut(s) 131
AciI CCGC 2 cut(s) 424, 459
AclWI GGATC 3 cut(s) 113, 458, 949
AcoI YGGCCR 1 cut(s) 48
AcsI RAATTY 3 cut(s) 265, 412, 815
AcyI GRCGYC 2 cut(s) 567, 573
AfaI GTAC 3 cut(s) 327, 800, 910
AfiI CCNNNNNNNGG 3 cut(s) 398, 425, 576
AflIII ACRYGT 1 cut(s) 328
AgeI ACCGGT 1 cut(s) 58
AgsI TTSAA 5 cut(s) 179, 409, 722, 815, 836
AhdI GACNNNNNGTC 1 cut(s) 984
AhlI ACTAGT 1 cut(s) 962
AjiI CACGTC 2 cut(s) 289, 656
AjnI CCWGG 2 cut(s) 50, 636
AluBI AGCT 5 cut(s) 8, 206, 375, 544, 706
AluI AGCT 5 cut(s) 8, 206, 375, 544, 706
Alw21I GWGCWC 1 cut(s) 718
Alw26I GTCTC 3 cut(s) 94, 846, 953
AlwI GGATC 3 cut(s) 113, 458, 949
Ama87I CYCGRG 1 cut(s) 419
AoxI GGCC 2 cut(s) 48, 151
ApeKI GCWGC 3 cut(s) 5, 703, 706
ApoI RAATTY 3 cut(s) 265, 412, 815
AsiGI ACCGGT 1 cut(s) 58
Asp700I GAANNNNTTC 1 cut(s) 375
AspLEI GCGC 2 cut(s) 105, 569
AspS9I GGNCC 2 cut(s) 152, 784
AsuC2I CCSGG 4 cut(s) 400, 420, 421, 564
AsuHPI GGTGA 1 cut(s) 518
AvaI CYCGRG 1 cut(s) 419
AvaII GGWCC 1 cut(s) 784
BaeI ACNNNNGTAYC 2 cut(s) 911, 944
BalI TGGCCA 1 cut(s) 50
BanI GGYRCC 1 cut(s) 566
BauI CACGAG 1 cut(s) 759
BbsI GAAGAC 1 cut(s) 925
Bbv12I GWGCWC 1 cut(s) 718
BbvI GCAGC 3 cut(s) 17, 693, 715
BccI CCATC 4 cut(s) 400, 496, 541, 581
BcgI CGANNNNNNTGC 2 cut(s) 66, 100
BciT130I CCWGG 2 cut(s) 52, 638
BcnI CCSGG 4 cut(s) 400, 420, 421, 564
BcoDI GTCTC 3 cut(s) 94, 846, 953
BcuI ACTAGT 1 cut(s) 962
BfaI CTAG 6 cut(s) 203, 620, 711, 728, 882, 963
BfoI RGCGCY 2 cut(s) 106, 570
BisI GCNGC 3 cut(s) 6, 704, 707
BlsI GCNGC 3 cut(s) 7, 705, 708
BmcAI AGTACT 1 cut(s) 910
Bme1390I CCNGG 6 cut(s) 52, 400, 420, 421, 564, 638
Bme18I GGWCC 1 cut(s) 784
BmeRI GACNNNNNGTC 1 cut(s) 984
BmeT110I CYCGRG 1 cut(s) 419
BmgBI CACGTC 2 cut(s) 289, 656
BmgT120I GGNCC 2 cut(s) 152, 784
BmiI GGNNCC 4 cut(s) 154, 389, 568, 746
BmrFI CCNGG 6 cut(s) 52, 400, 420, 421, 564, 638
BmrI ACTGGG 1 cut(s) 900
BmsI GCATC 6 cut(s) 68, 74, 169, 499, 595, 977
BmuI ACTGGG 1 cut(s) 900
BpiI GAAGAC 1 cut(s) 925
BpuMI CCSGG 4 cut(s) 400, 420, 421, 564
BsaAI YACGTR 1 cut(s) 329
BsaHI GRCGYC 2 cut(s) 567, 573
BsaJI CCNNGG 2 cut(s) 419, 748
BsaWI WCCGGW 1 cut(s) 58
Bsc4I CCNNNNNNNGG 3 cut(s) 398, 425, 576
Bse118I RCCGGY 1 cut(s) 58
Bse1I ACTGG 5 cut(s) 145, 292, 906, 989, 1026
Bse3DI GCAATG 2 cut(s) 316, 786
BseBI CCWGG 2 cut(s) 52, 638
BseDI CCNNGG 2 cut(s) 419, 748
BseGI GGATG 4 cut(s) 346, 392, 585, 1071
BseLI CCNNNNNNNGG 3 cut(s) 398, 425, 576
BseMI GCAATG 2 cut(s) 316, 786
BseMII CTCAG 1 cut(s) 779
BseNI ACTGG 5 cut(s) 145, 292, 906, 989, 1026
BseRI GAGGAG 1 cut(s) 701
BseXI GCAGC 3 cut(s) 17, 693, 715
BsgI GTGCAG 1 cut(s) 897
Bsh1236I CGCG 1 cut(s) 131
BshFI GGCC 2 cut(s) 50, 153
BshNI GGYRCC 1 cut(s) 566
BshTI ACCGGT 1 cut(s) 58
BsiHKAI GWGCWC 1 cut(s) 718
BsiHKCI CYCGRG 1 cut(s) 419
BsiSI CCGG 4 cut(s) 59, 399, 420, 564
BslI CCNNNNNNNGG 3 cut(s) 398, 425, 576
BsmAI GTCTC 3 cut(s) 94, 846, 953
BsmBI CGTCTC 1 cut(s) 94
BsmI GAATGC 1 cut(s) 14
BsnI GGCC 2 cut(s) 50, 153
BsoBI CYCGRG 1 cut(s) 419
Bsp1286I GDGCHC 1 cut(s) 718
Bsp1407I TGTACA 1 cut(s) 798
Bsp143I GATC 7 cut(s) 30, 118, 450, 513, 646, 884, 941
Bsp19I CCATGG 1 cut(s) 748
Bsp68I TCGCGA 1 cut(s) 131
BspACI CCGC 2 cut(s) 424, 459
BspANI GGCC 2 cut(s) 50, 153
BspCNI CTCAG 1 cut(s) 780
BspFNI CGCG 1 cut(s) 131
BspHI TCATGA 1 cut(s) 846
BspLI GGNNCC 4 cut(s) 154, 389, 568, 746
BspPI GGATC 3 cut(s) 113, 458, 949
BspQI GCTCTTC 1 cut(s) 723
BspT107I GGYRCC 1 cut(s) 566
BsrDI GCAATG 2 cut(s) 316, 786
BsrFI RCCGGY 1 cut(s) 58
BsrGI TGTACA 1 cut(s) 798
BsrI ACTGG 5 cut(s) 145, 292, 906, 989, 1026
BssAI RCCGGY 1 cut(s) 58
BssECI CCNNGG 2 cut(s) 419, 748
BssMI GATC 7 cut(s) 30, 118, 450, 513, 646, 884, 941
BssNAI GTATAC 1 cut(s) 1099
BssNI GRCGYC 2 cut(s) 567, 573
BssSI CACGAG 1 cut(s) 759
BssT1I CCWWGG 1 cut(s) 748
Bst1107I GTATAC 1 cut(s) 1099
Bst2BI CACGAG 1 cut(s) 759
Bst2UI CCWGG 2 cut(s) 52, 638
Bst4CI ACNGT 2 cut(s) 99, 986
Bst6I CTCTTC 4 cut(s) 21, 723, 1041, 1098
BstACI GRCGYC 2 cut(s) 567, 573
BstAPI GCANNNNNTGC 1 cut(s) 11
BstAUI TGTACA 1 cut(s) 798
BstBAI YACGTR 1 cut(s) 329
BstDEI CTNAG 1 cut(s) 788
BstDSI CCRYGG 1 cut(s) 748
BstF5I GGATG 4 cut(s) 346, 392, 585, 1071
BstFNI CGCG 1 cut(s) 131
BstH2I RGCGCY 2 cut(s) 106, 570
BstHHI GCGC 2 cut(s) 105, 569
BstKTI GATC 7 cut(s) 33, 121, 453, 516, 649, 887, 944
BstMAI GTCTC 3 cut(s) 94, 846, 953
BstMBI GATC 7 cut(s) 30, 118, 450, 513, 646, 884, 941
BstMWI GCNNNNNNNGC 1 cut(s) 11
BstNI CCWGG 2 cut(s) 52, 638
BstSCI CCNGG 6 cut(s) 50, 398, 418, 419, 562, 636
BstUI CGCG 1 cut(s) 131
BstV1I GCAGC 3 cut(s) 17, 693, 715
BstV2I GAAGAC 1 cut(s) 925
BstZ17I GTATAC 1 cut(s) 1099
BsuRI GGCC 2 cut(s) 50, 153
BtgI CCRYGG 1 cut(s) 748
BtgZI GCGATG 1 cut(s) 91
BtrI CACGTC 2 cut(s) 289, 656
BtsCI GGATG 4 cut(s) 346, 392, 585, 1071
BtsIMutI CAGTG 2 cut(s) 75, 978
BtuMI TCGCGA 1 cut(s) 131
CciI TCATGA 1 cut(s) 846
CfoI GCGC 2 cut(s) 105, 569
Cfr10I RCCGGY 1 cut(s) 58
Cfr13I GGNCC 2 cut(s) 152, 784
Cfr9I CCCGGG 1 cut(s) 419
CseI GACGC 1 cut(s) 76
Csp6I GTAC 3 cut(s) 326, 799, 909
CspAI ACCGGT 1 cut(s) 58
CviAII CATG 7 cut(s) 306, 338, 537, 749, 847, 1018, 1090
CviQI GTAC 3 cut(s) 326, 799, 909
DdeI CTNAG 1 cut(s) 788
DinI GGCGCC 1 cut(s) 568
DpnI GATC 7 cut(s) 32, 120, 452, 515, 648, 886, 943
DpnII GATC 7 cut(s) 30, 118, 450, 513, 646, 884, 941
DriI GACNNNNNGTC 1 cut(s) 984
EaeI YGGCCR 1 cut(s) 48
Eam1104I CTCTTC 4 cut(s) 21, 723, 1041, 1098
Eam1105I GACNNNNNGTC 1 cut(s) 984
EarI CTCTTC 4 cut(s) 21, 723, 1041, 1098
Eco130I CCWWGG 1 cut(s) 748
Eco47I GGWCC 1 cut(s) 784
Eco88I CYCGRG 1 cut(s) 419
EcoO109I RGGNCCY 1 cut(s) 152
EcoRI GAATTC 1 cut(s) 265
EcoRII CCWGG 2 cut(s) 50, 636
EcoT14I CCWWGG 1 cut(s) 748
EcoT22I ATGCAT 1 cut(s) 16
EgeI GGCGCC 1 cut(s) 568
EheI GGCGCC 1 cut(s) 568
ErhI CCWWGG 1 cut(s) 748
Esp3I CGTCTC 1 cut(s) 94
FaeI CATG 7 cut(s) 309, 341, 540, 752, 850, 1021, 1093
FatI CATG 7 cut(s) 305, 337, 536, 748, 846, 1017, 1089
FauI CCCGC 2 cut(s) 417, 452
FauNDI CATATG 2 cut(s) 432, 660
FblI GTMKAC 2 cut(s) 631, 1098
Fnu4HI GCNGC 3 cut(s) 6, 704, 707
FokI GGATG 4 cut(s) 333, 379, 592, 1078
Fsp4HI GCNGC 3 cut(s) 6, 704, 707
FspBI CTAG 6 cut(s) 203, 620, 711, 728, 882, 963
GlaI GCGC 2 cut(s) 104, 568
GluI GCNGC 3 cut(s) 6, 704, 707
HaeII RGCGCY 2 cut(s) 106, 570
HaeIII GGCC 2 cut(s) 50, 153
HapII CCGG 4 cut(s) 59, 399, 420, 564
HgaI GACGC 1 cut(s) 76
HhaI GCGC 2 cut(s) 105, 569
Hin1I GRCGYC 2 cut(s) 567, 573
Hin1II CATG 7 cut(s) 309, 341, 540, 752, 850, 1021, 1093
Hin6I GCGC 2 cut(s) 103, 567
HinP1I GCGC 2 cut(s) 103, 567
HincII GTYRAC 1 cut(s) 632
HindII GTYRAC 1 cut(s) 632
HindIII AAGCTT 1 cut(s) 373
HinfI GANTC 4 cut(s) 242, 533, 901, 1108
HpaII CCGG 4 cut(s) 59, 399, 420, 564
HphI GGTGA 1 cut(s) 518
Hpy166II GTNNAC 3 cut(s) 632, 799, 1099
Hpy188I TCNGA 8 cut(s) 118, 211, 257, 264, 358, 737, 958, 1057
Hpy8I GTNNAC 3 cut(s) 632, 799, 1099
Hpy99I CGWCG 2 cut(s) 488, 575
HpyAV CCTTC 2 cut(s) 232, 758
HpyCH4III ACNGT 2 cut(s) 99, 986
HpyCH4IV ACGT 5 cut(s) 288, 328, 573, 655, 740
HpyCH4V TGCA 8 cut(s) 14, 81, 309, 608, 703, 779, 914, 968
HpyF10VI GCNNNNNNNGC 1 cut(s) 11
HpyF3I CTNAG 1 cut(s) 788
HpySE526I ACGT 5 cut(s) 288, 328, 573, 655, 740
Hsp92I GRCGYC 2 cut(s) 567, 573
Hsp92II CATG 7 cut(s) 309, 341, 540, 752, 850, 1021, 1093
HspAI GCGC 2 cut(s) 103, 567
KasI GGCGCC 1 cut(s) 566
Kzo9I GATC 7 cut(s) 30, 118, 450, 513, 646, 884, 941
LguI GCTCTTC 1 cut(s) 723
Lsp1109I GCAGC 3 cut(s) 17, 693, 715
LweI GCATC 6 cut(s) 68, 74, 169, 499, 595, 977
MaeI CTAG 6 cut(s) 203, 620, 711, 728, 882, 963
MaeII ACGT 5 cut(s) 288, 328, 573, 655, 740
MaeIII GTNAC 2 cut(s) 218, 754
MalI GATC 7 cut(s) 32, 120, 452, 515, 648, 886, 943
MboI GATC 7 cut(s) 30, 118, 450, 513, 646, 884, 941
MboII GAAGA 7 cut(s) 38, 397, 710, 930, 1055, 1058, 1115
MhlI GDGCHC 1 cut(s) 718
MlsI TGGCCA 1 cut(s) 50
MluCI AATT 7 cut(s) 174, 265, 412, 815, 836, 945, 1006
MluNI TGGCCA 1 cut(s) 50
Mly113I GGCGCC 1 cut(s) 567
MlyI GAGTC 1 cut(s) 1117
MmeI TCCRAC 1 cut(s) 919
MnlI CCTC 3 cut(s) 194, 570, 679
Mox20I TGGCCA 1 cut(s) 50
Mph1103I ATGCAT 1 cut(s) 16
MroXI GAANNNNTTC 1 cut(s) 375
MscI TGGCCA 1 cut(s) 50
MseI TTAA 2 cut(s) 170, 215
MslI CAYNNNNRTG 2 cut(s) 663, 1064
Msp20I TGGCCA 1 cut(s) 50
MspA1I CMGCKG 1 cut(s) 706
MspI CCGG 4 cut(s) 59, 399, 420, 564
MspR9I CCNGG 6 cut(s) 52, 400, 420, 421, 564, 638
Mva1269I GAATGC 1 cut(s) 14
MvaI CCWGG 2 cut(s) 52, 638
MvnI CGCG 1 cut(s) 131
MwoI GCNNNNNNNGC 1 cut(s) 11
NarI GGCGCC 1 cut(s) 567
NciI CCSGG 4 cut(s) 400, 420, 421, 564
NcoI CCATGG 1 cut(s) 748
NdeI CATATG 2 cut(s) 432, 660
NdeII GATC 7 cut(s) 30, 118, 450, 513, 646, 884, 941
NlaIII CATG 7 cut(s) 309, 341, 540, 752, 850, 1021, 1093
NlaIV GGNNCC 4 cut(s) 154, 389, 568, 746
NruI TCGCGA 1 cut(s) 131
NsiI ATGCAT 1 cut(s) 16
PagI TCATGA 1 cut(s) 846
PciSI GCTCTTC 1 cut(s) 723
PctI GAATGC 1 cut(s) 14
PdmI GAANNNNTTC 1 cut(s) 375
PfeI GAWTC 3 cut(s) 242, 533, 901
PinAI ACCGGT 1 cut(s) 58
PkrI GCNGC 3 cut(s) 7, 705, 708
PleI GAGTC 1 cut(s) 1116
PluTI GGCGCC 1 cut(s) 570
PpsI GAGTC 1 cut(s) 1116
Ppu21I YACGTR 1 cut(s) 329
PsiI TTATAA 1 cut(s) 1011
Psp6I CCWGG 2 cut(s) 50, 636
PspGI CCWGG 2 cut(s) 50, 636
PspN4I GGNNCC 4 cut(s) 154, 389, 568, 746
PspPI GGNCC 2 cut(s) 152, 784
PvuII CAGCTG 1 cut(s) 706
RruI TCGCGA 1 cut(s) 131
RsaI GTAC 3 cut(s) 327, 800, 910
RsaNI GTAC 3 cut(s) 326, 799, 909
RseI CAYNNNNRTG 2 cut(s) 663, 1064
SalI GTCGAC 1 cut(s) 630
SapI GCTCTTC 1 cut(s) 723
SaqAI TTAA 2 cut(s) 170, 215
SatI GCNGC 3 cut(s) 6, 704, 707
Sau3AI GATC 7 cut(s) 30, 118, 450, 513, 646, 884, 941
Sau96I GGNCC 2 cut(s) 152, 784
ScaI AGTACT 1 cut(s) 910
SchI GAGTC 1 cut(s) 1117
ScrFI CCNGG 6 cut(s) 52, 400, 420, 421, 564, 638
SduI GDGCHC 1 cut(s) 718
SfaNI GCATC 6 cut(s) 68, 74, 169, 499, 595, 977
SfoI GGCGCC 1 cut(s) 568
SinI GGWCC 1 cut(s) 784
SmaI CCCGGG 1 cut(s) 421
SmiMI CAYNNNNRTG 2 cut(s) 663, 1064
SpeI ACTAGT 1 cut(s) 962
Sse9I AATT 7 cut(s) 174, 265, 412, 815, 836, 945, 1006
SsiI CCGC 2 cut(s) 424, 459
SspDI GGCGCC 1 cut(s) 566
SspI AATATT 1 cut(s) 555
SspMI CTAG 6 cut(s) 203, 620, 711, 728, 882, 963
StyD4I CCNGG 6 cut(s) 50, 398, 418, 419, 562, 636
StyI CCWWGG 1 cut(s) 748
TaaI ACNGT 2 cut(s) 99, 986
TaiI ACGT 5 cut(s) 291, 331, 576, 658, 743
TaqI TCGA 6 cut(s) 163, 195, 234, 631, 672, 695
TasI AATT 7 cut(s) 174, 265, 412, 815, 836, 945, 1006
TatI WGTACW 2 cut(s) 798, 908
TfiI GAWTC 3 cut(s) 242, 533, 901
Tru1I TTAA 2 cut(s) 170, 215
Tru9I TTAA 2 cut(s) 170, 215
TscAI CASTG 2 cut(s) 75, 978
TseI GCWGC 3 cut(s) 5, 703, 706
TspDTI ATGAA 4 cut(s) 326, 335, 1056, 1095
TspMI CCCGGG 1 cut(s) 419
TspRI CASTG 2 cut(s) 75, 978
VpaK11BI GGWCC 1 cut(s) 784
XapI RAATTY 3 cut(s) 265, 412, 815
XmaI CCCGGG 1 cut(s) 419
XmiI GTMKAC 2 cut(s) 631, 1098
XmnI GAANNNNTTC 1 cut(s) 375
XspI CTAG 6 cut(s) 203, 620, 711, 728, 882, 963
ZraI GACGTC 1 cut(s) 574
ZrmI AGTACT 1 cut(s) 910
Zsp2I ATGCAT 1 cut(s) 16
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.