Rmu_ssc0000355.1_g000021

F-box kelch-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000355.1
OG0001674
Physical Location & Seq
Forward (+)
80812 .. 81990
1179 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_ssc0000355.1_g000021.1.cds

Sequence Viewer

Length: 1179 bp
atgaagagaattctgaagtcatctgagtcaagtgcctcagctggtgtctctttcaaatcagtagccatggtggcagctgcaatattacccgaagagataatagtgcagatcgtgaccaggctacctgtcaaatctgtggggcgatgcagatgcgtctgtaaacggtggcgcactctattctctgatccccaattcgccaaattgcacttccaatgtgcccctaagcagcaaaccatactattcttccgcaactttgaccttgaaatcctaaatttgagtcataatctgtcgttcggaaaggaatctctagttctccgaaatctgaatgtccccttcgatcaccggtgggctgtctttataggctcctgcaatgggctggtagcatcatatctctcccgttccttcctgcaacaggaaaggctgtttatatggaacccgtccaccagattctttaagaaactacccagccgaggtaatacttgtcagatatccatttttgggtttggctatgtgtctgccaccgacgagtacaaagctgtgataaatggggagatcttctcatccaagagtgaatcatggaagcggattgaaacccctcgcttccgcatcgtgtcatcatgtggtgtgcttttcggtgaggcactccattggctattctatcccgatgttgaaaggcaacaggttccaaatctgtatgctttcgatttggagaaggaggagttttcagtaatgaagctgcctagtgctcttcaaacacacgggcttagaaactgttttatatgccagggtggtgtttcttgtgaagggttcttgtatgtaatggtcaagaaggcgcggtgggattgtggtaagttggcctattatgaaattgacttttgggtcatgagagagtatggcaaaaatgattcctggactagatcgttcagcgtaaggattcccggtcagcctgagcggagggactggtatcttttgattcaagcaatttcggttagggaagctagtgcatttgtgaggaatagatgtccaagggaagaagagaagcttgacatgtctatacttcattatcgtaatgaagaagagaactccgacatgtatatgcttgacaatgatgcacaagacatggttgtatatgaagagagtctgttgtctttaatagtatcatgccgtggaggactctaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like FBA_1 FBA_3

Protein Analysis

392

Amino Acids

45.32

Weight (kDa)

8.71

Isoelectric Point (pI)

54.57

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 878
AccBSI CCGCTC 1 cut(s) 952
AccII CGCG 1 cut(s) 835
AciI CCGC 5 cut(s) 247, 583, 604, 835, 952
AclWI GGATC 1 cut(s) 179
AcsI RAATTY 2 cut(s) 9, 271
AcuI CTGAAG 1 cut(s) 35
AfaI GTAC 1 cut(s) 530
AfiI CCNNNNNNNGG 4 cut(s) 372, 412, 470, 498
AflIII ACRYGT 2 cut(s) 1047, 1089
AgeI ACCGGT 1 cut(s) 342
AgsI TTSAA 6 cut(s) 55, 263, 590, 671, 752, 977
AjnI CCWGG 3 cut(s) 116, 783, 908
AjuI GAANNNNNNNTTGG 2 cut(s) 191, 223
AluBI AGCT 6 cut(s) 41, 77, 536, 736, 998, 1042
AluI AGCT 6 cut(s) 41, 77, 536, 736, 998, 1042
Alw21I GWGCWC 1 cut(s) 748
Alw26I GTCTC 1 cut(s) 52
AlwI GGATC 1 cut(s) 179
AoxI GGCC 1 cut(s) 855
ApeKI GCWGC 4 cut(s) 74, 77, 226, 736
ApoI RAATTY 2 cut(s) 9, 271
AsiGI ACCGGT 1 cut(s) 342
AspLEI GCGC 2 cut(s) 171, 835
AsuC2I CCSGG 1 cut(s) 939
AsuHPI GGTGA 2 cut(s) 332, 647
BaeGI GKGCMC 1 cut(s) 220
Bbv12I GWGCWC 1 cut(s) 748
BbvCI CCTCAGC 1 cut(s) 37
BbvI GCAGC 4 cut(s) 64, 86, 238, 723
BceAI ACGGC 1 cut(s) 1149
BcgI CGANNNNNNTGC 2 cut(s) 132, 166
BciT130I CCWGG 3 cut(s) 118, 785, 910
BcnI CCSGG 1 cut(s) 939
BcoDI GTCTC 1 cut(s) 52
BfaI CTAG 4 cut(s) 308, 741, 915, 999
BglI GCCNNNNNGGC 1 cut(s) 71
BglII AGATCT 1 cut(s) 552
BisI GCNGC 4 cut(s) 75, 78, 227, 737
BlsI GCNGC 4 cut(s) 76, 79, 228, 738
Bme1390I CCNGG 4 cut(s) 118, 785, 910, 939
BmiI GGNNCC 3 cut(s) 364, 434, 684
BmrFI CCNGG 4 cut(s) 118, 785, 910, 939
BmsI GCATC 5 cut(s) 134, 140, 392, 615, 1099
BplI GAGNNNNNCTC 2 cut(s) 542, 574
Bpu10I CCTNAGC 3 cut(s) 37, 222, 948
BpuMI CCSGG 1 cut(s) 939
BsaJI CCNNGG 5 cut(s) 66, 469, 784, 1025, 1165
BsaWI WCCGGW 1 cut(s) 342
Bsc4I CCNNNNNNNGG 4 cut(s) 372, 412, 470, 498
Bse118I RCCGGY 1 cut(s) 342
Bse1I ACTGG 1 cut(s) 965
Bse3DI GCAATG 1 cut(s) 376
BseBI CCWGG 3 cut(s) 118, 785, 910
BseDI CCNNGG 5 cut(s) 66, 469, 784, 1025, 1165
BseGI GGATG 1 cut(s) 560
BseLI CCNNNNNNNGG 4 cut(s) 372, 412, 470, 498
BseMI GCAATG 1 cut(s) 376
BseMII CTCAG 3 cut(s) 15, 51, 939
BseNI ACTGG 1 cut(s) 965
BseRI GAGGAG 1 cut(s) 731
BseSI GKGCMC 1 cut(s) 220
BseXI GCAGC 4 cut(s) 64, 86, 238, 723
BseYI CCCAGC 1 cut(s) 464
BsgI GTGCAG 1 cut(s) 125
Bsh1236I CGCG 1 cut(s) 835
BshFI GGCC 1 cut(s) 857
BshTI ACCGGT 1 cut(s) 342
BsiHKAI GWGCWC 1 cut(s) 748
BsiSI CCGG 2 cut(s) 343, 939
BslFI GGGAC 2 cut(s) 314, 971
BslI CCNNNNNNNGG 4 cut(s) 372, 412, 470, 498
BsmAI GTCTC 1 cut(s) 52
BsmFI GGGAC 2 cut(s) 314, 971
BsnI GGCC 1 cut(s) 857
Bsp1286I GDGCHC 2 cut(s) 220, 748
Bsp143I GATC 5 cut(s) 108, 184, 337, 552, 917
Bsp19I CCATGG 1 cut(s) 66
BspACI CCGC 5 cut(s) 247, 583, 604, 835, 952
BspANI GGCC 1 cut(s) 857
BspCNI CTCAG 3 cut(s) 16, 50, 940
BspFNI CGCG 1 cut(s) 835
BspHI TCATGA 1 cut(s) 882
BspLI GGNNCC 3 cut(s) 364, 434, 684
BspPI GGATC 1 cut(s) 179
BspQI GCTCTTC 1 cut(s) 753
BsrBI CCGCTC 1 cut(s) 952
BsrDI GCAATG 1 cut(s) 376
BsrFI RCCGGY 1 cut(s) 342
BsrI ACTGG 1 cut(s) 965
BssAI RCCGGY 1 cut(s) 342
BssECI CCNNGG 5 cut(s) 66, 469, 784, 1025, 1165
BssMI GATC 5 cut(s) 108, 184, 337, 552, 917
BssT1I CCWWGG 2 cut(s) 66, 1025
Bst2UI CCWGG 3 cut(s) 118, 785, 910
Bst4CI ACNGT 2 cut(s) 165, 773
Bst6I CTCTTC 5 cut(s) 87, 753, 1029, 1071, 1128
BstDEI CTNAG 5 cut(s) 24, 37, 222, 764, 948
BstDSI CCRYGG 2 cut(s) 66, 1165
BstENI CCTNNNNNAGG 1 cut(s) 410
BstF5I GGATG 1 cut(s) 560
BstFNI CGCG 1 cut(s) 835
BstHHI GCGC 2 cut(s) 171, 835
BstKTI GATC 5 cut(s) 111, 187, 340, 555, 920
BstMAI GTCTC 1 cut(s) 52
BstMBI GATC 5 cut(s) 108, 184, 337, 552, 917
BstMWI GCNNNNNNNGC 1 cut(s) 71
BstNI CCWGG 3 cut(s) 118, 785, 910
BstNSI RCATGY 2 cut(s) 1051, 1093
BstSCI CCNGG 4 cut(s) 116, 783, 908, 937
BstSLI GKGCMC 1 cut(s) 220
BstUI CGCG 1 cut(s) 835
BstV1I GCAGC 4 cut(s) 64, 86, 238, 723
BstX2I RGATCY 1 cut(s) 552
BstYI RGATCY 1 cut(s) 552
BsuRI GGCC 1 cut(s) 857
BtgI CCRYGG 2 cut(s) 66, 1165
BtgZI GCGATG 1 cut(s) 157
BtsCI GGATG 1 cut(s) 560
CciI TCATGA 1 cut(s) 882
CfoI GCGC 2 cut(s) 171, 835
Cfr10I RCCGGY 1 cut(s) 342
CseI GACGC 1 cut(s) 142
Csp6I GTAC 1 cut(s) 529
CspAI ACCGGT 1 cut(s) 342
CviAII CATG 8 cut(s) 67, 576, 618, 883, 1048, 1090, 1120, 1161
CviQI GTAC 1 cut(s) 529
DdeI CTNAG 5 cut(s) 24, 37, 222, 764, 948
DpnI GATC 5 cut(s) 110, 186, 339, 554, 919
DpnII GATC 5 cut(s) 108, 184, 337, 552, 917
DrdI GACNNNNNNGTC 1 cut(s) 878
DseDI GACNNNNNNGTC 1 cut(s) 878
Eam1104I CTCTTC 5 cut(s) 87, 753, 1029, 1071, 1128
EarI CTCTTC 5 cut(s) 87, 753, 1029, 1071, 1128
Eco130I CCWWGG 2 cut(s) 66, 1025
Eco32I GATATC 1 cut(s) 489
Eco57I CTGAAG 1 cut(s) 35
EcoNI CCTNNNNNAGG 1 cut(s) 410
EcoRI GAATTC 1 cut(s) 9
EcoRII CCWGG 3 cut(s) 116, 783, 908
EcoRV GATATC 1 cut(s) 489
EcoT14I CCWWGG 2 cut(s) 66, 1025
ErhI CCWWGG 2 cut(s) 66, 1025
FaeI CATG 8 cut(s) 70, 579, 621, 886, 1051, 1093, 1123, 1164
FalI AAGNNNNNCTT 2 cut(s) 1026, 1058
FaqI GGGAC 2 cut(s) 314, 971
FatI CATG 8 cut(s) 66, 575, 617, 882, 1047, 1089, 1119, 1160
Fnu4HI GCNGC 4 cut(s) 75, 78, 227, 737
FokI GGATG 1 cut(s) 547
Fsp4HI GCNGC 4 cut(s) 75, 78, 227, 737
FspBI CTAG 4 cut(s) 308, 741, 915, 999
GlaI GCGC 2 cut(s) 170, 834
GluI GCNGC 4 cut(s) 75, 78, 227, 737
GsaI CCCAGC 1 cut(s) 468
HaeIII GGCC 1 cut(s) 857
HapII CCGG 2 cut(s) 343, 939
HgaI GACGC 1 cut(s) 142
HhaI GCGC 2 cut(s) 171, 835
Hin1II CATG 8 cut(s) 70, 579, 621, 886, 1051, 1093, 1123, 1164
Hin6I GCGC 2 cut(s) 169, 833
HinP1I GCGC 2 cut(s) 169, 833
HindIII AAGCTT 1 cut(s) 1040
HpaII CCGG 2 cut(s) 343, 939
HphI GGTGA 2 cut(s) 332, 647
Hpy166II GTNNAC 2 cut(s) 161, 441
Hpy188I TCNGA 8 cut(s) 15, 25, 184, 296, 317, 324, 486, 1087
Hpy188III TCNNGA 4 cut(s) 112, 662, 826, 883
Hpy8I GTNNAC 2 cut(s) 161, 441
Hpy99I CGWCG 1 cut(s) 527
HpyAV CCTTC 5 cut(s) 343, 412, 706, 797, 823
HpyCH4III ACNGT 2 cut(s) 165, 773
HpyCH4V TGCA 8 cut(s) 80, 106, 147, 205, 369, 409, 1004, 1112
HpyF10VI GCNNNNNNNGC 1 cut(s) 71
HpyF3I CTNAG 5 cut(s) 24, 37, 222, 764, 948
Hsp92II CATG 8 cut(s) 70, 579, 621, 886, 1051, 1093, 1123, 1164
HspAI GCGC 2 cut(s) 169, 833
Kzo9I GATC 5 cut(s) 108, 184, 337, 552, 917
LguI GCTCTTC 1 cut(s) 753
LmnI GCTCC 1 cut(s) 368
Lsp1109I GCAGC 4 cut(s) 64, 86, 238, 723
LweI GCATC 5 cut(s) 134, 140, 392, 615, 1099
MaeI CTAG 4 cut(s) 308, 741, 915, 999
MaeIII GTNAC 1 cut(s) 112
MalI GATC 5 cut(s) 110, 186, 339, 554, 919
MbiI CCGCTC 1 cut(s) 952
MboI GATC 5 cut(s) 108, 184, 337, 552, 917
MflI RGATCY 1 cut(s) 552
MhlI GDGCHC 2 cut(s) 220, 748
MluCI AATT 6 cut(s) 9, 191, 200, 271, 867, 981
MlyI GAGTC 4 cut(s) 35, 286, 1147, 1167
MmeI TCCRAC 1 cut(s) 1110
MnlI CCTC 8 cut(s) 46, 464, 606, 631, 709, 948, 1005, 1163
MseI TTAA 2 cut(s) 453, 1151
MslI CAYNNNNRTG 1 cut(s) 1094
MspA1I CMGCKG 2 cut(s) 41, 77
MspI CCGG 2 cut(s) 343, 939
MspR9I CCNGG 4 cut(s) 118, 785, 910, 939
MvaI CCWGG 3 cut(s) 118, 785, 910
MvnI CGCG 1 cut(s) 835
MwoI GCNNNNNNNGC 1 cut(s) 71
NciI CCSGG 1 cut(s) 939
NcoI CCATGG 1 cut(s) 66
NdeII GATC 5 cut(s) 108, 184, 337, 552, 917
NlaIII CATG 8 cut(s) 70, 579, 621, 886, 1051, 1093, 1123, 1164
NlaIV GGNNCC 3 cut(s) 364, 434, 684
NmeAIII GCCGAG 1 cut(s) 494
NmuCI GTSAC 1 cut(s) 112
NspI RCATGY 2 cut(s) 1051, 1093
PagI TCATGA 1 cut(s) 882
PciI ACATGT 2 cut(s) 1047, 1089
PciSI GCTCTTC 1 cut(s) 753
PfeI GAWTC 6 cut(s) 302, 447, 572, 905, 934, 973
PfoI TCCNGGA 1 cut(s) 908
PinAI ACCGGT 1 cut(s) 342
PkrI GCNGC 4 cut(s) 76, 79, 228, 738
PleI GAGTC 4 cut(s) 34, 285, 1146, 1167
PpsI GAGTC 4 cut(s) 34, 285, 1146, 1167
PscI ACATGT 2 cut(s) 1047, 1089
Psp6I CCWGG 3 cut(s) 116, 783, 908
PspFI CCCAGC 1 cut(s) 464
PspGI CCWGG 3 cut(s) 116, 783, 908
PspN4I GGNNCC 3 cut(s) 364, 434, 684
PsuI RGATCY 1 cut(s) 552
PvuII CAGCTG 2 cut(s) 41, 77
RsaI GTAC 1 cut(s) 530
RsaNI GTAC 1 cut(s) 529
RseI CAYNNNNRTG 1 cut(s) 1094
SapI GCTCTTC 1 cut(s) 753
SaqAI TTAA 2 cut(s) 453, 1151
SatI GCNGC 4 cut(s) 75, 78, 227, 737
Sau3AI GATC 5 cut(s) 108, 184, 337, 552, 917
SchI GAGTC 4 cut(s) 35, 286, 1147, 1167
ScrFI CCNGG 4 cut(s) 118, 785, 910, 939
SduI GDGCHC 2 cut(s) 220, 748
SfaNI GCATC 5 cut(s) 134, 140, 392, 615, 1099
SgrAI CRCCGGYG 1 cut(s) 342
SmiMI CAYNNNNRTG 1 cut(s) 1094
Sse9I AATT 6 cut(s) 9, 191, 200, 271, 867, 981
SsiI CCGC 5 cut(s) 247, 583, 604, 835, 952
SspI AATATT 1 cut(s) 84
SspMI CTAG 4 cut(s) 308, 741, 915, 999
StyD4I CCNGG 4 cut(s) 116, 783, 908, 937
StyI CCWWGG 2 cut(s) 66, 1025
TaaI ACNGT 2 cut(s) 165, 773
TaqI TCGA 2 cut(s) 336, 702
TasI AATT 6 cut(s) 9, 191, 200, 271, 867, 981
TatI WGTACW 1 cut(s) 528
TfiI GAWTC 6 cut(s) 302, 447, 572, 905, 934, 973
Tru1I TTAA 2 cut(s) 453, 1151
Tru9I TTAA 2 cut(s) 453, 1151
TseFI GTSAC 1 cut(s) 112
TseI GCWGC 4 cut(s) 74, 77, 226, 736
Tsp45I GTSAC 1 cut(s) 112
TspDTI ATGAA 6 cut(s) 17, 746, 879, 1049, 1086, 1146
XagI CCTNNNNNAGG 1 cut(s) 410
XapI RAATTY 2 cut(s) 9, 271
XceI RCATGY 2 cut(s) 1051, 1093
XspI CTAG 4 cut(s) 308, 741, 915, 999
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.