pycom02g00940
ERF Family

Belongs to the calycin superfamily. Lipocalin family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0001674
Physical Location & Seq
Reverse (-)
550307 .. 551781
1475 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g00940.2

Sequence Viewer

Length: 813 bp
ATGCGCTGGACTAGGAGAAAGGGGTTCGTCCCTTTCCGGGAAGTTCAACCATTCACACTAAGGCCCAGAAACTCCCACGTCCCCGTCCTTTCACTTTACCTTCTGCCCGAAGCAAAAAACCCATTAAAACTCCGCCACCTGCACACACCTTATCCTCACAAAACCCCCCACCCAAAATTCACGAACTCCCGCCCCAATTTCCTCACACACTCTCGCACAAACCAAAACACAAACACTGCATCTGCAACAATGGCGACGAAGAAGTTGATGGAAGTCGTGAAGGGCCTGGATGTGCAGCGCTACATGGGGCGGTGGTACGAGATTGCCTCCTTTCCGTCGCTGTTTCAGCCCAAGAACGGCGAGAACACGCGGGCGACGTACACTCTGAAAGAAGACGGCGCCACAGTGAATGTTTTGAACGAGACTTGGACCGACGGGAAGAGGGGGTTCATCGAGGGCACCGCCTACAAGGCTGACCCGGCGAGCGACGAGGCGAAGCTGAAGGTGAAGTTTTATGTGCCTCCATTTCTGCCGATCATCCCTGTCGTTGGGGATTACTGGGTTTTGTTCATCGATGAGGATTATCAGTATGCGCTGATTGGCCAACCTAGCAGGAATTATCTTTGGATATTGAGCAGGCAGCCTTATCTGGATGAAGAAATATACAACCAGCTGGTTCAGAGGGCGGCGGACGAGGGTTACGACGTGAGCAAACTCCACAAGACACCGCAGAGCAAGACTCCGCCGGAGGCGGAAGAAGGCCCCAAAGACACCATGGGGATCTGGTGGTTCAAATCCATCTTCGGAAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0009279 GO:0016020 GO:0019867 GO:0030312 GO:0030313 GO:0031975 GO:0044462 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipocalin_2

Protein Analysis

271

Amino Acids

31.34

Weight (kDa)

9.48

Isoelectric Point (pI)

42.68

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 147
Acc36I ACCTGC 1 cut(s) 147
AccB1I GGYRCC 2 cut(s) 398, 458
AccII CGCG 1 cut(s) 370
AclWI GGATC 1 cut(s) 788
AcoI YGGCCR 1 cut(s) 601
AcsI RAATTY 1 cut(s) 176
AcuI CTGAAG 1 cut(s) 521
AcyI GRCGYC 1 cut(s) 399
AfaI GTAC 2 cut(s) 317, 380
AfeI AGCGCT 1 cut(s) 299
AfiI CCNNNNNNNGG 3 cut(s) 37, 356, 548
AgsI TTSAA 3 cut(s) 47, 418, 793
AjiI CACGTC 2 cut(s) 79, 706
AjnI CCWGG 1 cut(s) 285
AluBI AGCT 2 cut(s) 499, 673
AluI AGCT 2 cut(s) 499, 673
Alw26I GTCTC 1 cut(s) 416
AlwI GGATC 1 cut(s) 788
Aor51HI AGCGCT 1 cut(s) 299
AoxI GGCC 4 cut(s) 62, 283, 601, 760
ApeKI GCWGC 2 cut(s) 295, 640
ApoI RAATTY 1 cut(s) 176
AspLEI GCGC 4 cut(s) 6, 300, 401, 595
AspS9I GGNCC 4 cut(s) 63, 283, 429, 761
AsuC2I CCSGG 2 cut(s) 38, 479
AsuHPI GGTGA 1 cut(s) 517
AvaII GGWCC 1 cut(s) 429
BaeGI GKGCMC 1 cut(s) 461
BalI TGGCCA 1 cut(s) 603
BanI GGYRCC 2 cut(s) 398, 458
BbsI GAAGAC 1 cut(s) 399
BbvI GCAGC 2 cut(s) 307, 652
BccI CCATC 2 cut(s) 262, 806
BceAI ACGGC 2 cut(s) 373, 412
BciT130I CCWGG 1 cut(s) 287
BcnI CCSGG 2 cut(s) 38, 479
BcoDI GTCTC 1 cut(s) 416
BfaI CTAG 2 cut(s) 12, 609
BfoI RGCGCY 2 cut(s) 301, 402
BfuAI ACCTGC 1 cut(s) 147
BglI GCCNNNNNGGC 1 cut(s) 470
BisI GCNGC 3 cut(s) 296, 641, 687
BlsI GCNGC 3 cut(s) 297, 642, 688
Bme1390I CCNGG 3 cut(s) 38, 287, 479
Bme18I GGWCC 1 cut(s) 429
BmgBI CACGTC 2 cut(s) 79, 706
BmgT120I GGNCC 4 cut(s) 63, 283, 429, 761
BmiI GGNNCC 3 cut(s) 400, 460, 763
BmrFI CCNGG 3 cut(s) 38, 287, 479
BmrI ACTGGG 1 cut(s) 568
BmsI GCATC 1 cut(s) 248
BmuI ACTGGG 1 cut(s) 568
BpiI GAAGAC 1 cut(s) 399
BplI GAGNNNNNCTC 4 cut(s) 311, 343, 724, 756
BpuMI CCSGG 2 cut(s) 38, 479
Bsa29I ATCGAT 1 cut(s) 573
BsaHI GRCGYC 1 cut(s) 399
BsaJI CCNNGG 1 cut(s) 774
Bsc4I CCNNNNNNNGG 3 cut(s) 37, 356, 548
Bse1I ACTGG 1 cut(s) 563
BseBI CCWGG 1 cut(s) 287
BseCI ATCGAT 1 cut(s) 573
BseDI CCNNGG 1 cut(s) 774
BseGI GGATG 3 cut(s) 295, 537, 658
BseLI CCNNNNNNNGG 3 cut(s) 37, 356, 548
BseNI ACTGG 1 cut(s) 563
BseSI GKGCMC 1 cut(s) 461
BseXI GCAGC 2 cut(s) 307, 652
BsgI GTGCAG 2 cut(s) 125, 314
Bsh1236I CGCG 1 cut(s) 370
BshFI GGCC 4 cut(s) 64, 285, 603, 762
BshNI GGYRCC 2 cut(s) 398, 458
BshVI ATCGAT 1 cut(s) 573
BsiSI CCGG 3 cut(s) 37, 479, 746
BslFI GGGAC 2 cut(s) 14, 65
BslI CCNNNNNNNGG 3 cut(s) 37, 356, 548
BsmAI GTCTC 1 cut(s) 416
BsmFI GGGAC 2 cut(s) 14, 65
BsnI GGCC 4 cut(s) 64, 285, 603, 762
Bsp1286I GDGCHC 1 cut(s) 461
Bsp143I GATC 2 cut(s) 534, 780
Bsp19I CCATGG 1 cut(s) 774
BspANI GGCC 4 cut(s) 64, 285, 603, 762
BspDI ATCGAT 1 cut(s) 573
BspFNI CGCG 1 cut(s) 370
BspLI GGNNCC 3 cut(s) 400, 460, 763
BspMI ACCTGC 1 cut(s) 147
BspPI GGATC 1 cut(s) 788
BspT107I GGYRCC 2 cut(s) 398, 458
BsrI ACTGG 1 cut(s) 563
BssECI CCNNGG 1 cut(s) 774
BssMI GATC 2 cut(s) 534, 780
BssNI GRCGYC 1 cut(s) 399
BssT1I CCWWGG 1 cut(s) 774
Bst2UI CCWGG 1 cut(s) 287
Bst4CI ACNGT 1 cut(s) 406
Bst6I CTCTTC 1 cut(s) 434
BstACI GRCGYC 1 cut(s) 399
BstC8I GCNNGC 3 cut(s) 372, 484, 638
BstDEI CTNAG 1 cut(s) 59
BstDSI CCRYGG 1 cut(s) 774
BstF5I GGATG 3 cut(s) 295, 537, 658
BstFNI CGCG 1 cut(s) 370
BstH2I RGCGCY 2 cut(s) 301, 402
BstHHI GCGC 4 cut(s) 6, 300, 401, 595
BstKTI GATC 2 cut(s) 537, 783
BstMAI GTCTC 1 cut(s) 416
BstMBI GATC 2 cut(s) 534, 780
BstMWI GCNNNNNNNGC 5 cut(s) 251, 346, 470, 479, 609
BstNI CCWGG 1 cut(s) 287
BstSCI CCNGG 3 cut(s) 36, 285, 477
BstSLI GKGCMC 1 cut(s) 461
BstUI CGCG 1 cut(s) 370
BstV1I GCAGC 2 cut(s) 307, 652
BstV2I GAAGAC 1 cut(s) 399
BstX2I RGATCY 1 cut(s) 780
BstYI RGATCY 1 cut(s) 780
Bsu15I ATCGAT 1 cut(s) 573
BsuRI GGCC 4 cut(s) 64, 285, 603, 762
BsuTUI ATCGAT 1 cut(s) 573
BtgI CCRYGG 1 cut(s) 774
BtrI CACGTC 2 cut(s) 79, 706
BtsCI GGATG 3 cut(s) 295, 537, 658
BtsI GCAGTG 1 cut(s) 234
BtsIMutI CAGTG 2 cut(s) 234, 411
BveI ACCTGC 1 cut(s) 147
Cac8I GCNNGC 3 cut(s) 372, 484, 638
CfoI GCGC 4 cut(s) 6, 300, 401, 595
Cfr13I GGNCC 4 cut(s) 63, 283, 429, 761
ClaI ATCGAT 1 cut(s) 573
Csp6I GTAC 2 cut(s) 316, 379
CviAII CATG 2 cut(s) 304, 775
CviJI RGCY 9 cut(s) 64, 285, 349, 473, 499, 603, 643, 673, 762
CviKI_1 RGCY 9 cut(s) 64, 285, 349, 473, 499, 603, 643, 673, 762
CviQI GTAC 2 cut(s) 316, 379
DdeI CTNAG 1 cut(s) 59
DinI GGCGCC 1 cut(s) 400
DpnI GATC 2 cut(s) 536, 782
DpnII GATC 2 cut(s) 534, 780
EaeI YGGCCR 1 cut(s) 601
Eam1104I CTCTTC 1 cut(s) 434
EarI CTCTTC 1 cut(s) 434
EciI GGCGGA 4 cut(s) 122, 704, 732, 767
Eco130I CCWWGG 1 cut(s) 774
Eco47I GGWCC 1 cut(s) 429
Eco47III AGCGCT 1 cut(s) 299
Eco57I CTGAAG 1 cut(s) 521
EcoO109I RGGNCCY 2 cut(s) 283, 761
EcoRII CCWGG 1 cut(s) 285
EcoT14I CCWWGG 1 cut(s) 774
EgeI GGCGCC 1 cut(s) 400
EheI GGCGCC 1 cut(s) 400
ErhI CCWWGG 1 cut(s) 774
FaeI CATG 2 cut(s) 307, 778
FaiI YATR 5 cut(s) 305, 516, 591, 664, 776
FaqI GGGAC 2 cut(s) 14, 65
FatI CATG 2 cut(s) 303, 774
FauI CCCGC 2 cut(s) 197, 363
Fnu4HI GCNGC 3 cut(s) 296, 641, 687
FokI GGATG 3 cut(s) 302, 524, 665
Fsp4HI GCNGC 3 cut(s) 296, 641, 687
FspBI CTAG 2 cut(s) 12, 609
GlaI GCGC 4 cut(s) 5, 299, 400, 594
GluI GCNGC 3 cut(s) 296, 641, 687
HaeII RGCGCY 2 cut(s) 301, 402
HaeIII GGCC 4 cut(s) 64, 285, 603, 762
HapII CCGG 3 cut(s) 37, 479, 746
HhaI GCGC 4 cut(s) 6, 300, 401, 595
Hin1I GRCGYC 1 cut(s) 399
Hin1II CATG 2 cut(s) 307, 778
Hin6I GCGC 4 cut(s) 4, 298, 399, 593
HinP1I GCGC 4 cut(s) 4, 298, 399, 593
HinfI GANTC 1 cut(s) 739
HpaII CCGG 3 cut(s) 37, 479, 746
HphI GGTGA 1 cut(s) 517
Hpy166II GTNNAC 1 cut(s) 381
Hpy188I TCNGA 3 cut(s) 387, 681, 806
Hpy188III TCNNGA 3 cut(s) 181, 277, 650
Hpy8I GTNNAC 1 cut(s) 381
Hpy99I CGWCG 6 cut(s) 259, 340, 379, 437, 491, 707
HpyAV CCTTC 4 cut(s) 110, 274, 496, 752
HpyCH4III ACNGT 1 cut(s) 406
HpyCH4IV ACGT 3 cut(s) 78, 377, 705
HpyCH4V TGCA 4 cut(s) 142, 239, 245, 295
HpyF10VI GCNNNNNNNGC 5 cut(s) 251, 346, 470, 479, 609
HpyF3I CTNAG 1 cut(s) 59
HpySE526I ACGT 3 cut(s) 78, 377, 705
Hsp92I GRCGYC 1 cut(s) 399
Hsp92II CATG 2 cut(s) 307, 778
HspAI GCGC 4 cut(s) 4, 298, 399, 593
KasI GGCGCC 1 cut(s) 398
Kzo9I GATC 2 cut(s) 534, 780
Lsp1109I GCAGC 2 cut(s) 307, 652
LweI GCATC 1 cut(s) 248
MaeI CTAG 2 cut(s) 12, 609
MaeII ACGT 3 cut(s) 78, 377, 705
MaeIII GTNAC 1 cut(s) 698
MalI GATC 2 cut(s) 536, 782
MboI GATC 2 cut(s) 534, 780
MboII GAAGA 6 cut(s) 271, 404, 451, 668, 767, 793
MflI RGATCY 1 cut(s) 780
MhlI GDGCHC 1 cut(s) 461
MlsI TGGCCA 1 cut(s) 603
MluCI AATT 3 cut(s) 176, 196, 616
MluNI TGGCCA 1 cut(s) 603
Mly113I GGCGCC 1 cut(s) 399
MlyI GAGTC 1 cut(s) 733
Mox20I TGGCCA 1 cut(s) 603
MscI TGGCCA 1 cut(s) 603
MseI TTAA 1 cut(s) 125
Msp20I TGGCCA 1 cut(s) 603
MspA1I CMGCKG 1 cut(s) 673
MspI CCGG 3 cut(s) 37, 479, 746
MspR9I CCNGG 3 cut(s) 38, 287, 479
MvaI CCWGG 1 cut(s) 287
MvnI CGCG 1 cut(s) 370
MwoI GCNNNNNNNGC 5 cut(s) 251, 346, 470, 479, 609
NarI GGCGCC 1 cut(s) 399
NciI CCSGG 2 cut(s) 38, 479
NcoI CCATGG 1 cut(s) 774
NdeII GATC 2 cut(s) 534, 780
NlaIII CATG 2 cut(s) 307, 778
NlaIV GGNNCC 3 cut(s) 400, 460, 763
PaqCI CACCTGC 1 cut(s) 147
PcsI WCGNNNNNNNCGW 2 cut(s) 374, 699
PfoI TCCNGGA 1 cut(s) 36
PkrI GCNGC 3 cut(s) 297, 642, 688
PleI GAGTC 1 cut(s) 733
PluTI GGCGCC 1 cut(s) 402
PpsI GAGTC 1 cut(s) 733
Psp6I CCWGG 1 cut(s) 285
PspGI CCWGG 1 cut(s) 285
PspN4I GGNNCC 3 cut(s) 400, 460, 763
PspPI GGNCC 4 cut(s) 63, 283, 429, 761
PsuI RGATCY 1 cut(s) 780
PvuII CAGCTG 1 cut(s) 673
RsaI GTAC 2 cut(s) 317, 380
RsaNI GTAC 2 cut(s) 316, 379
SaqAI TTAA 1 cut(s) 125
SatI GCNGC 3 cut(s) 296, 641, 687
Sau3AI GATC 2 cut(s) 534, 780
Sau96I GGNCC 4 cut(s) 63, 283, 429, 761
SchI GAGTC 1 cut(s) 733
ScrFI CCNGG 3 cut(s) 38, 287, 479
SduI GDGCHC 1 cut(s) 461
SfaNI GCATC 1 cut(s) 248
SfoI GGCGCC 1 cut(s) 400
SinI GGWCC 1 cut(s) 429
Sse9I AATT 3 cut(s) 176, 196, 616
SspDI GGCGCC 1 cut(s) 398
SspMI CTAG 2 cut(s) 12, 609
StyD4I CCNGG 3 cut(s) 36, 285, 477
StyI CCWWGG 1 cut(s) 774
TaaI ACNGT 1 cut(s) 406
TaiI ACGT 3 cut(s) 81, 380, 708
TaqI TCGA 2 cut(s) 453, 573
TaqII GACCGA 1 cut(s) 446
TasI AATT 3 cut(s) 176, 196, 616
TauI GCSGC 1 cut(s) 689
Tru1I TTAA 1 cut(s) 125
Tru9I TTAA 1 cut(s) 125
TscAI CASTG 2 cut(s) 241, 411
TseI GCWGC 2 cut(s) 295, 640
TspDTI ATGAA 3 cut(s) 439, 559, 669
TspGWI ACGGA 1 cut(s) 324
TspRI CASTG 2 cut(s) 241, 411
VpaK11BI GGWCC 1 cut(s) 429
XapI RAATTY 1 cut(s) 176
XcmI CCANNNNNNNNNTGG 1 cut(s) 772
XspI CTAG 2 cut(s) 12, 609
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.