RchiOBHm_Chr2g0122651

F-box kelch-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0001674
Physical Location & Seq
Forward (+)
35681888 .. 35683093
1206 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ49502

Sequence Viewer

Length: 1206 bp
ATGGCCCGCATGAAGTCAACTGCAATAATACCTGAAGATATTATAGTAGAAATTGTGATGAGGTTGCCGATCAAACCCGTAGGTCGATGCAGGTGCGTTTGTAAACGGTGGCGCTCTCTATTCTCCGGCCTGAAGTTTGCCAAGTCGCACATACAAACAGCTTTTGAGCGGCAAACCCTCTGTCAAAGACTTTTTCTCCTCACCCATGAATACGAGTCGCTAGACTTGGAGTTGGGGGAGCTAAAAGAGCTAAAGAAATCTTCACTCAGAGTCGAACAGCTGAATGCCCCATTCAAGAAACCGGGCCATGCTCTCTGCTTTTTAGGTTCATGCAATGGGTTGGTAGCATGTTTTAGTGGTGATCTTATTCTGAAAAATGGTTGTTTCACTCCTAAAAGTCGTTTATTTCAAAAAGAAAGCTTGTTTATCTGGAACCCGTCAACCGGATTCTACAAAGAAATACCTGGGGAAGGCTTTTATCTTCCATGGGGAGTTTCCACGCGTGGTTTTGGCCATGTCTCCGGCACCGACGACTATAAAGTTGTGTTAAGAGGGAAGAGGCCGAGGGAGGTCAGCCTCTTCTCGTTGAGAGCTGGGACGTGGAAATCGATTAAATCTCCTGTGATCGGCACTATGCAAAGAAAGGGGCTGTTTTTGAGAGAGGCACTTCATTGGCTAGCTTATGAGCGCGACAACACTGCTCCTTTTAAGTATTCCGGACTACCAATAGTACTCTTTGCCTTTGATTTGGCCAAGGAGGAGTTCCGGAAAATGCAGGTGCCTAGTAGTAGAATTCTGGACTTGACTTATGAAAGCCGTTTGGGAGTTTCTTGTGAAGGATGCCTGTACATAATGCATTATAGTCGGCTGTTTGAGCCTAAATGCATATATTATGTTGTTGATTTTTGGGTCATGAGAGAATACGGCAACAGTGATTCCTGGACTAGATCCTTCAGCCTAAGGTTTTCCGATGAGCCCAATAAGCCGATTTATATGCTTGAACCGATCTTGCTTAAGGAAACTAGTGCATTTGTGATGAACAGACCGAAGAAGTTTGATCAAGACAAAGAAGAGATGCCTGATCATCACATGTATATGATTCATGATCAAAATAAACAAGAGAACGCCAGCATGTATATTCTTCACAGTAATCCACCAGACATGATTGTATATGAAGAGAGTCTCAGTGACTATCATAAGGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like FBA_1 FBA_3

Protein Analysis

401

Amino Acids

46.58

Weight (kDa)

8.95

Isoelectric Point (pI)

46.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 10 - 46 2.3e-09 F-box domain
F-box-like PF12937 10 - 41 2.2e-06 F-box-like
FBA_3 PF08268 128 - 316 1.3e-07 F-box associated beta propeller domain
FBA_1 PF07734 132 - 395 2.2e-14 F-box associated beta propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 81, 766
Acc36I ACCTGC 2 cut(s) 81, 766
AccB1I GGYRCC 2 cut(s) 524, 778
AccBSI CCGCTC 1 cut(s) 169
AccII CGCG 2 cut(s) 502, 690
AccIII TCCGGA 2 cut(s) 716, 765
AciI CCGC 2 cut(s) 7, 169
AclWI GGATC 1 cut(s) 942
AcoI YGGCCR 2 cut(s) 511, 750
AcsI RAATTY 1 cut(s) 792
AcuI CTGAAG 3 cut(s) 54, 152, 937
AfaI GTAC 2 cut(s) 732, 848
AfiI CCNNNNNNNGG 3 cut(s) 443, 470, 626
AflII CTTAAG 1 cut(s) 1013
AflIII ACRYGT 2 cut(s) 500, 1089
AgsI TTSAA 3 cut(s) 295, 410, 1001
AhlI ACTAGT 1 cut(s) 1022
AjiI CACGTC 1 cut(s) 600
AjnI CCWGG 2 cut(s) 463, 938
AjuI GAANNNNNNNTTGG 2 cut(s) 746, 778
AluBI AGCT 7 cut(s) 161, 241, 250, 280, 420, 593, 680
AluI AGCT 7 cut(s) 161, 241, 250, 280, 420, 593, 680
Alw26I GTCTC 2 cut(s) 523, 1187
AlwI GGATC 1 cut(s) 942
Aor13HI TCCGGA 2 cut(s) 716, 765
AoxI GGCC 6 cut(s) 3, 127, 304, 511, 560, 750
ApoI RAATTY 1 cut(s) 792
ArsI GACNNNNNNTTYG 2 cut(s) 166, 198
AspLEI GCGC 2 cut(s) 114, 690
AspS9I GGNCC 2 cut(s) 4, 304
AsuC2I CCSGG 1 cut(s) 303
AsuHPI GGTGA 2 cut(s) 193, 371
AsuNHI GCTAGC 1 cut(s) 676
AxyI CCTNAGG 1 cut(s) 959
BalI TGGCCA 2 cut(s) 513, 752
BanI GGYRCC 2 cut(s) 524, 778
BanII GRGCYC 1 cut(s) 978
BceAI ACGGC 2 cut(s) 801, 940
BcgI CGANNNNNNTGC 8 cut(s) 75, 109, 680, 714, 845, 879, 976, 1010
BciT130I CCWGG 2 cut(s) 465, 940
BclI TGATCA 3 cut(s) 1057, 1081, 1105
BcnI CCSGG 1 cut(s) 303
BcoDI GTCTC 2 cut(s) 523, 1187
BcuI ACTAGT 1 cut(s) 1022
BfaI CTAG 5 cut(s) 221, 677, 783, 945, 1023
BfoI RGCGCY 1 cut(s) 115
BfrI CTTAAG 1 cut(s) 1013
BfuAI ACCTGC 2 cut(s) 81, 766
BisI GCNGC 1 cut(s) 170
BlsI GCNGC 1 cut(s) 171
BmcAI AGTACT 1 cut(s) 732
Bme1390I CCNGG 3 cut(s) 303, 465, 940
BmgBI CACGTC 1 cut(s) 600
BmgT120I GGNCC 2 cut(s) 4, 304
BmiI GGNNCC 3 cut(s) 434, 526, 780
BmrFI CCNGG 3 cut(s) 303, 465, 940
BmsI GCATC 3 cut(s) 77, 830, 1065
BmtI GCTAGC 1 cut(s) 680
BpuMI CCSGG 1 cut(s) 303
Bsa29I ATCGAT 1 cut(s) 608
BsaJI CCNNGG 4 cut(s) 464, 485, 563, 753
BsaWI WCCGGW 3 cut(s) 443, 716, 765
BsaXI ACNNNNNCTCC 2 cut(s) 180, 210
Bsc4I CCNNNNNNNGG 3 cut(s) 443, 470, 626
Bse21I CCTNAGG 1 cut(s) 959
Bse3DI GCAATG 1 cut(s) 340
BseAI TCCGGA 2 cut(s) 716, 765
BseBI CCWGG 2 cut(s) 465, 940
BseCI ATCGAT 1 cut(s) 608
BseDI CCNNGG 4 cut(s) 464, 485, 563, 753
BseGI GGATG 1 cut(s) 845
BseLI CCNNNNNNNGG 3 cut(s) 443, 470, 626
BseMI GCAATG 1 cut(s) 340
BseMII CTCAG 2 cut(s) 280, 1198
BseRI GAGGAG 2 cut(s) 188, 773
BseYI CCCAGC 1 cut(s) 593
Bsh1236I CGCG 2 cut(s) 502, 690
BshFI GGCC 6 cut(s) 5, 129, 306, 513, 562, 752
BshNI GGYRCC 2 cut(s) 524, 778
BshVI ATCGAT 1 cut(s) 608
BsiSI CCGG 6 cut(s) 126, 302, 444, 522, 717, 766
BslFI GGGAC 1 cut(s) 610
BslI CCNNNNNNNGG 3 cut(s) 443, 470, 626
BsmAI GTCTC 2 cut(s) 523, 1187
BsmFI GGGAC 1 cut(s) 610
BsmI GAATGC 1 cut(s) 289
BsnI GGCC 6 cut(s) 5, 129, 306, 513, 562, 752
Bsp1286I GDGCHC 1 cut(s) 978
Bsp13I TCCGGA 2 cut(s) 716, 765
Bsp1407I TGTACA 1 cut(s) 846
Bsp143I GATC 8 cut(s) 69, 361, 624, 947, 1005, 1057, 1081, 1105
Bsp19I CCATGG 1 cut(s) 485
BspACI CCGC 2 cut(s) 7, 169
BspANI GGCC 6 cut(s) 5, 129, 306, 513, 562, 752
BspCNI CTCAG 2 cut(s) 279, 1197
BspDI ATCGAT 1 cut(s) 608
BspEI TCCGGA 2 cut(s) 716, 765
BspFNI CGCG 2 cut(s) 502, 690
BspHI TCATGA 2 cut(s) 912, 1102
BspLI GGNNCC 3 cut(s) 434, 526, 780
BspMI ACCTGC 2 cut(s) 81, 766
BspOI GCTAGC 1 cut(s) 680
BspPI GGATC 1 cut(s) 942
BspT107I GGYRCC 2 cut(s) 524, 778
BspTI CTTAAG 1 cut(s) 1013
BsrBI CCGCTC 1 cut(s) 169
BsrDI GCAATG 1 cut(s) 340
BsrGI TGTACA 1 cut(s) 846
BssECI CCNNGG 4 cut(s) 464, 485, 563, 753
BssMI GATC 8 cut(s) 69, 361, 624, 947, 1005, 1057, 1081, 1105
BssT1I CCWWGG 2 cut(s) 485, 753
Bst2UI CCWGG 2 cut(s) 465, 940
Bst4CI ACNGT 3 cut(s) 108, 932, 1148
Bst6I CTCTTC 4 cut(s) 551, 584, 1065, 1170
BstAFI CTTAAG 1 cut(s) 1013
BstAUI TGTACA 1 cut(s) 846
BstC8I GCNNGC 3 cut(s) 7, 678, 1129
BstDEI CTNAG 3 cut(s) 266, 959, 1184
BstDSI CCRYGG 1 cut(s) 485
BstENI CCTNNNNNAGG 1 cut(s) 468
BstF5I GGATG 1 cut(s) 845
BstFNI CGCG 2 cut(s) 502, 690
BstH2I RGCGCY 1 cut(s) 115
BstHHI GCGC 2 cut(s) 114, 690
BstKTI GATC 8 cut(s) 72, 364, 627, 950, 1008, 1060, 1084, 1108
BstMAI GTCTC 2 cut(s) 523, 1187
BstMBI GATC 8 cut(s) 69, 361, 624, 947, 1005, 1057, 1081, 1105
BstMWI GCNNNNNNNGC 3 cut(s) 247, 874, 982
BstNI CCWGG 2 cut(s) 465, 940
BstNSI RCATGY 3 cut(s) 351, 1093, 1135
BstSCI CCNGG 3 cut(s) 301, 463, 938
BstUI CGCG 2 cut(s) 502, 690
BstX2I RGATCY 1 cut(s) 947
BstYI RGATCY 1 cut(s) 947
Bsu15I ATCGAT 1 cut(s) 608
Bsu36I CCTNAGG 1 cut(s) 959
BsuRI GGCC 6 cut(s) 5, 129, 306, 513, 562, 752
BsuTUI ATCGAT 1 cut(s) 608
BtgI CCRYGG 1 cut(s) 485
BtrI CACGTC 1 cut(s) 600
BtsCI GGATG 1 cut(s) 845
BtsI GCAGTG 1 cut(s) 696
BtsIMutI CAGTG 3 cut(s) 696, 937, 1192
BveI ACCTGC 2 cut(s) 81, 766
Cac8I GCNNGC 3 cut(s) 7, 678, 1129
CciI TCATGA 2 cut(s) 912, 1102
CfoI GCGC 2 cut(s) 114, 690
Cfr13I GGNCC 2 cut(s) 4, 304
ClaI ATCGAT 1 cut(s) 608
Csp6I GTAC 2 cut(s) 731, 847
CviQI GTAC 2 cut(s) 731, 847
DdeI CTNAG 3 cut(s) 266, 959, 1184
DpnI GATC 8 cut(s) 71, 363, 626, 949, 1007, 1059, 1083, 1107
DpnII GATC 8 cut(s) 69, 361, 624, 947, 1005, 1057, 1081, 1105
EaeI YGGCCR 2 cut(s) 511, 750
Eam1104I CTCTTC 4 cut(s) 551, 584, 1065, 1170
EarI CTCTTC 4 cut(s) 551, 584, 1065, 1170
Eco130I CCWWGG 2 cut(s) 485, 753
Eco24I GRGCYC 1 cut(s) 978
Eco57I CTGAAG 3 cut(s) 54, 152, 937
Eco81I CCTNAGG 1 cut(s) 959
EcoNI CCTNNNNNAGG 1 cut(s) 468
EcoRI GAATTC 1 cut(s) 792
EcoRII CCWGG 2 cut(s) 463, 938
EcoT14I CCWWGG 2 cut(s) 485, 753
EcoT22I ATGCAT 2 cut(s) 858, 887
EcoT38I GRGCYC 1 cut(s) 978
ErhI CCWWGG 2 cut(s) 485, 753
FaqI GGGAC 1 cut(s) 610
FauI CCCGC 1 cut(s) 14
FbaI TGATCA 3 cut(s) 1057, 1081, 1105
Fnu4HI GCNGC 1 cut(s) 170
FokI GGATG 1 cut(s) 852
FriOI GRGCYC 1 cut(s) 978
Fsp4HI GCNGC 1 cut(s) 170
FspBI CTAG 5 cut(s) 221, 677, 783, 945, 1023
GlaI GCGC 2 cut(s) 113, 689
GluI GCNGC 1 cut(s) 170
GsaI CCCAGC 1 cut(s) 597
HaeII RGCGCY 1 cut(s) 115
HaeIII GGCC 6 cut(s) 5, 129, 306, 513, 562, 752
HapII CCGG 6 cut(s) 126, 302, 444, 522, 717, 766
HhaI GCGC 2 cut(s) 114, 690
Hin6I GCGC 2 cut(s) 112, 688
HinP1I GCGC 2 cut(s) 112, 688
HincII GTYRAC 2 cut(s) 18, 441
HindII GTYRAC 2 cut(s) 18, 441
HindIII AAGCTT 1 cut(s) 418
HinfI GANTC 6 cut(s) 215, 270, 447, 935, 1099, 1180
HpaII CCGG 6 cut(s) 126, 302, 444, 522, 717, 766
HphI GGTGA 2 cut(s) 193, 371
Hpy166II GTNNAC 3 cut(s) 18, 104, 441
Hpy188I TCNGA 3 cut(s) 269, 372, 970
Hpy188III TCNNGA 8 cut(s) 295, 430, 717, 766, 797, 913, 1061, 1103
Hpy8I GTNNAC 3 cut(s) 18, 104, 441
Hpy99I CGWCG 1 cut(s) 533
HpyAV CCTTC 3 cut(s) 464, 830, 961
HpyCH4III ACNGT 3 cut(s) 108, 932, 1148
HpyCH4IV ACGT 1 cut(s) 599
HpyCH4V TGCA 8 cut(s) 23, 90, 333, 637, 775, 856, 885, 1028
HpyF10VI GCNNNNNNNGC 3 cut(s) 247, 874, 982
HpyF3I CTNAG 3 cut(s) 266, 959, 1184
HpySE526I ACGT 1 cut(s) 599
HspAI GCGC 2 cut(s) 112, 688
Kpn2I TCCGGA 2 cut(s) 716, 765
Ksp22I TGATCA 3 cut(s) 1057, 1081, 1105
Kzo9I GATC 8 cut(s) 69, 361, 624, 947, 1005, 1057, 1081, 1105
LmnI GCTCC 2 cut(s) 238, 706
LweI GCATC 3 cut(s) 77, 830, 1065
MaeI CTAG 5 cut(s) 221, 677, 783, 945, 1023
MaeII ACGT 1 cut(s) 599
MaeIII GTNAC 1 cut(s) 1187
MalI GATC 8 cut(s) 71, 363, 626, 949, 1007, 1059, 1083, 1107
MbiI CCGCTC 1 cut(s) 169
MboI GATC 8 cut(s) 69, 361, 624, 947, 1005, 1057, 1081, 1105
MboII GAAGA 9 cut(s) 47, 252, 473, 568, 571, 1060, 1082, 1133, 1187
MflI RGATCY 1 cut(s) 947
MhlI GDGCHC 1 cut(s) 978
MlsI TGGCCA 2 cut(s) 513, 752
MluCI AATT 2 cut(s) 51, 792
MluI ACGCGT 1 cut(s) 500
MluNI TGGCCA 2 cut(s) 513, 752
MlyI GAGTC 3 cut(s) 224, 279, 1189
Mox20I TGGCCA 2 cut(s) 513, 752
Mph1103I ATGCAT 2 cut(s) 858, 887
MroI TCCGGA 2 cut(s) 716, 765
MscI TGGCCA 2 cut(s) 513, 752
MseI TTAA 4 cut(s) 548, 612, 708, 1014
MslI CAYNNNNRTG 1 cut(s) 1094
Msp20I TGGCCA 2 cut(s) 513, 752
MspA1I CMGCKG 1 cut(s) 280
MspCI CTTAAG 1 cut(s) 1013
MspI CCGG 6 cut(s) 126, 302, 444, 522, 717, 766
MspR9I CCNGG 3 cut(s) 303, 465, 940
Mva1269I GAATGC 1 cut(s) 289
MvaI CCWGG 2 cut(s) 465, 940
MvnI CGCG 2 cut(s) 502, 690
MwoI GCNNNNNNNGC 3 cut(s) 247, 874, 982
NciI CCSGG 1 cut(s) 303
NcoI CCATGG 1 cut(s) 485
NdeII GATC 8 cut(s) 69, 361, 624, 947, 1005, 1057, 1081, 1105
NheI GCTAGC 1 cut(s) 676
NlaIV GGNNCC 3 cut(s) 434, 526, 780
NmeAIII GCCGAG 1 cut(s) 588
NmuCI GTSAC 1 cut(s) 1187
NsiI ATGCAT 2 cut(s) 858, 887
NspI RCATGY 3 cut(s) 351, 1093, 1135
PagI TCATGA 2 cut(s) 912, 1102
PaqCI CACCTGC 2 cut(s) 81, 766
PciI ACATGT 1 cut(s) 1089
PcsI WCGNNNNNNNCGW 1 cut(s) 605
PctI GAATGC 1 cut(s) 289
PfeI GAWTC 3 cut(s) 447, 935, 1099
PfoI TCCNGGA 1 cut(s) 938
PkrI GCNGC 1 cut(s) 171
PleI GAGTC 3 cut(s) 223, 278, 1188
PpsI GAGTC 3 cut(s) 223, 278, 1188
PscI ACATGT 1 cut(s) 1089
Psp6I CCWGG 2 cut(s) 463, 938
PspFI CCCAGC 1 cut(s) 593
PspGI CCWGG 2 cut(s) 463, 938
PspN4I GGNNCC 3 cut(s) 434, 526, 780
PspPI GGNCC 2 cut(s) 4, 304
PsuI RGATCY 1 cut(s) 947
PvuII CAGCTG 1 cut(s) 280
RsaI GTAC 2 cut(s) 732, 848
RsaNI GTAC 2 cut(s) 731, 847
RseI CAYNNNNRTG 1 cut(s) 1094
SaqAI TTAA 4 cut(s) 548, 612, 708, 1014
SatI GCNGC 1 cut(s) 170
Sau3AI GATC 8 cut(s) 69, 361, 624, 947, 1005, 1057, 1081, 1105
Sau96I GGNCC 2 cut(s) 4, 304
ScaI AGTACT 1 cut(s) 732
SchI GAGTC 3 cut(s) 224, 279, 1189
ScrFI CCNGG 3 cut(s) 303, 465, 940
SduI GDGCHC 1 cut(s) 978
SfaNI GCATC 3 cut(s) 77, 830, 1065
SmiMI CAYNNNNRTG 1 cut(s) 1094
SmlI CTYRAG 1 cut(s) 1013
SmoI CTYRAG 1 cut(s) 1013
SpeI ACTAGT 1 cut(s) 1022
Sse9I AATT 2 cut(s) 51, 792
SsiI CCGC 2 cut(s) 7, 169
SspMI CTAG 5 cut(s) 221, 677, 783, 945, 1023
StyD4I CCNGG 3 cut(s) 301, 463, 938
StyI CCWWGG 2 cut(s) 485, 753
TaaI ACNGT 3 cut(s) 108, 932, 1148
TaiI ACGT 1 cut(s) 602
TaqI TCGA 3 cut(s) 85, 273, 608
TaqII GACCGA 1 cut(s) 1060
TasI AATT 2 cut(s) 51, 792
TatI WGTACW 2 cut(s) 730, 846
TauI GCSGC 1 cut(s) 172
TfiI GAWTC 3 cut(s) 447, 935, 1099
Tru1I TTAA 4 cut(s) 548, 612, 708, 1014
Tru9I TTAA 4 cut(s) 548, 612, 708, 1014
TscAI CASTG 3 cut(s) 703, 937, 1192
TseFI GTSAC 1 cut(s) 1187
Tsp45I GTSAC 1 cut(s) 1187
TspDTI ATGAA 8 cut(s) 26, 222, 318, 659, 825, 1052, 1091, 1188
TspRI CASTG 3 cut(s) 703, 937, 1192
Vha464I CTTAAG 1 cut(s) 1013
XagI CCTNNNNNAGG 1 cut(s) 468
XapI RAATTY 1 cut(s) 792
XceI RCATGY 3 cut(s) 351, 1093, 1135
XspI CTAG 5 cut(s) 221, 677, 783, 945, 1023
ZrmI AGTACT 1 cut(s) 732
Zsp2I ATGCAT 2 cut(s) 858, 887
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.