FvH4_1g25891

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0001040
Physical Location & Seq
Reverse (-)
17803598 .. 17804813
1216 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g25891.t1

Sequence Viewer

Length: 867 bp
ATGGAGAGTAGTAGCATTTTGGATTTTATTGACACACCAGCGCTCTTCCCCTGGGTGGAGGAGAAGGAAGAGTATTTGTACATTCAATTCAAGGACCATCTGACCCAGGTGAATCTTGACCAACTGGAGCGGAAGGAGGAACCTAAGTTGGAAATAGTTGCAGATTACCGAACCAAGGGTCTCCCCCTAGGAATGTGTCCGTTCTTTGAGATTTGTGACCAATCCGATTTCCTCAATTCTCAAATTTTTATTTTGGATCCCTCCACCGGAAACATCACTGTTGATACTCGTTTTCCCAAACCGAATGCAGCCAAGACCAATCCCAGAGTCTTGGCTACCATTGACCAAAAGGTTTACGTACTCGGTACTCATGCTTATTACTATGAAGACGTGCTTCCAAATCCGACGTTCGAGTGCTTCGATGTTATTCAACAAAGGTGGGAGGCTCTGCCTGCTCCTCCTTTTTATGATGAACATCCTTACACAGATCGACACGTGCCTCGTTTTGACCCTGATTCAGACTATGAAGAAGATTTCGGCGACTACAAGTCTGAGTGTCTGACGCGGCTCCAACATCATTTTAATCATCTTCCTTCTCCGAATCCGAGAAGGTTTGGGGGAAAAGTTGTTCATGTGGGTGGTGACAAGTTTTGCCTCATTTTGTCAGGAAGAATTAACAATCGGTCTCGGTCGTTGGCTGTGGCAACCTTTCAGCTAACCTTTAGTATGCTGTTTTTTGATCTTCTATTCAACCAGGTTGAGCTATCTTATCATCAGTATGATGACATTGATTCAATAACCGCTAATCTTCATGCACAAGAGAAGGACCACAAGTCTCCAATGCCATATGATTTCACAAGTTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

289

Amino Acids

33.54

Weight (kDa)

4.86

Isoelectric Point (pI)

54.2

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 130
AccII CGCG 1 cut(s) 565
AciI CCGC 3 cut(s) 130, 565, 801
AclWI GGATC 2 cut(s) 251, 264
AcsI RAATTY 1 cut(s) 243
AcvI CACGTG 1 cut(s) 496
AfaI GTAC 3 cut(s) 80, 360, 367
AfeI AGCGCT 1 cut(s) 42
AfiI CCNNNNNNNGG 3 cut(s) 55, 175, 266
AflIII ACRYGT 1 cut(s) 493
AgsI TTSAA 5 cut(s) 86, 91, 431, 751, 795
AhdI GACNNNNNGTC 2 cut(s) 547, 832
AjiI CACGTC 1 cut(s) 391
AjnI CCWGG 3 cut(s) 50, 105, 753
AloI GAACNNNNNNTCC 2 cut(s) 612, 644
AluBI AGCT 2 cut(s) 715, 763
AluI AGCT 2 cut(s) 715, 763
Alw26I GTCTC 3 cut(s) 185, 690, 840
AlwI GGATC 2 cut(s) 251, 264
Aor51HI AGCGCT 1 cut(s) 42
ApeKI GCWGC 1 cut(s) 308
ApoI RAATTY 1 cut(s) 243
ArsI GACNNNNNNTTYG 2 cut(s) 163, 195
AspA2I CCTAGG 1 cut(s) 187
AspLEI GCGC 1 cut(s) 43
AspS9I GGNCC 2 cut(s) 94, 826
AsuHPI GGTGA 2 cut(s) 121, 653
AvaII GGWCC 2 cut(s) 94, 826
AvrII CCTAGG 1 cut(s) 187
BamHI GGATCC 1 cut(s) 256
BbrPI CACGTG 1 cut(s) 496
BbsI GAAGAC 1 cut(s) 393
BbvI GCAGC 1 cut(s) 320
BccI CCATC 1 cut(s) 105
BciT130I CCWGG 3 cut(s) 52, 107, 755
BcoDI GTCTC 3 cut(s) 185, 690, 840
BfaI CTAG 1 cut(s) 188
BfoI RGCGCY 1 cut(s) 44
BisI GCNGC 2 cut(s) 309, 566
BlnI CCTAGG 1 cut(s) 187
BlsI GCNGC 2 cut(s) 310, 567
Bme1390I CCNGG 3 cut(s) 52, 107, 755
Bme18I GGWCC 2 cut(s) 94, 826
BmeRI GACNNNNNGTC 2 cut(s) 547, 832
BmgBI CACGTC 1 cut(s) 391
BmgT120I GGNCC 2 cut(s) 94, 826
BmiI GGNNCC 3 cut(s) 141, 258, 569
BmrFI CCNGG 3 cut(s) 52, 107, 755
BpiI GAAGAC 1 cut(s) 393
BpmI CTGGAG 1 cut(s) 146
BsaAI YACGTR 2 cut(s) 358, 496
BsaBI GATNNNNATC 1 cut(s) 474
BsaI GGTCTC 2 cut(s) 185, 690
BsaJI CCNNGG 5 cut(s) 50, 51, 105, 174, 187
BsaWI WCCGGW 1 cut(s) 266
Bsc4I CCNNNNNNNGG 3 cut(s) 55, 175, 266
Bse1I ACTGG 1 cut(s) 129
Bse8I GATNNNNATC 1 cut(s) 474
BseBI CCWGG 3 cut(s) 52, 107, 755
BseDI CCNNGG 5 cut(s) 50, 51, 105, 174, 187
BseGI GGATG 1 cut(s) 475
BseJI GATNNNNATC 1 cut(s) 474
BseLI CCNNNNNNNGG 3 cut(s) 55, 175, 266
BseMII CTCAG 1 cut(s) 543
BseNI ACTGG 1 cut(s) 129
BseRI GAGGAG 2 cut(s) 74, 447
BseXI GCAGC 1 cut(s) 320
Bsh1236I CGCG 1 cut(s) 565
Bsh1285I CGRYCG 1 cut(s) 692
BsiEI CGRYCG 1 cut(s) 692
BsiSI CCGG 1 cut(s) 267
BslI CCNNNNNNNGG 3 cut(s) 55, 175, 266
BsmAI GTCTC 3 cut(s) 185, 690, 840
BsmI GAATGC 1 cut(s) 310
Bso31I GGTCTC 2 cut(s) 185, 690
Bsp1407I TGTACA 1 cut(s) 78
Bsp143I GATC 3 cut(s) 256, 487, 739
BspACI CCGC 3 cut(s) 130, 565, 801
BspCNI CTCAG 1 cut(s) 544
BspFNI CGCG 1 cut(s) 565
BspLI GGNNCC 3 cut(s) 141, 258, 569
BspPI GGATC 2 cut(s) 251, 264
BspQI GCTCTTC 1 cut(s) 50
BspTNI GGTCTC 2 cut(s) 185, 690
BsrBI CCGCTC 1 cut(s) 130
BsrGI TGTACA 1 cut(s) 78
BsrI ACTGG 1 cut(s) 129
BssECI CCNNGG 5 cut(s) 50, 51, 105, 174, 187
BssMI GATC 3 cut(s) 256, 487, 739
BssT1I CCWWGG 2 cut(s) 174, 187
Bst2UI CCWGG 3 cut(s) 52, 107, 755
Bst4CI ACNGT 1 cut(s) 280
Bst6I CTCTTC 2 cut(s) 50, 63
BstAUI TGTACA 1 cut(s) 78
BstBAI YACGTR 2 cut(s) 358, 496
BstC8I GCNNGC 1 cut(s) 453
BstDEI CTNAG 2 cut(s) 144, 552
BstF5I GGATG 1 cut(s) 475
BstFNI CGCG 1 cut(s) 565
BstH2I RGCGCY 1 cut(s) 44
BstHHI GCGC 1 cut(s) 43
BstKTI GATC 3 cut(s) 259, 490, 742
BstMAI GTCTC 3 cut(s) 185, 690, 840
BstMBI GATC 3 cut(s) 256, 487, 739
BstMCI CGRYCG 1 cut(s) 692
BstMWI GCNNNNNNNGC 1 cut(s) 452
BstNI CCWGG 3 cut(s) 52, 107, 755
BstSCI CCNGG 3 cut(s) 50, 105, 753
BstSNI TACGTA 1 cut(s) 358
BstUI CGCG 1 cut(s) 565
BstV1I GCAGC 1 cut(s) 320
BstV2I GAAGAC 1 cut(s) 393
BstX2I RGATCY 1 cut(s) 256
BstXI CCANNNNNNTGG 1 cut(s) 331
BstYI RGATCY 1 cut(s) 256
BtrI CACGTC 1 cut(s) 391
BtsCI GGATG 1 cut(s) 475
BtsIMutI CAGTG 1 cut(s) 276
Cac8I GCNNGC 1 cut(s) 453
CfoI GCGC 1 cut(s) 43
Cfr13I GGNCC 2 cut(s) 94, 826
CseI GACGC 1 cut(s) 571
CsiI ACCWGGT 1 cut(s) 753
Csp6I GTAC 3 cut(s) 79, 359, 366
CspCI CAANNNNNGTGG 2 cut(s) 419, 454
CviAII CATG 3 cut(s) 371, 632, 812
CviJI RGCY 7 cut(s) 311, 335, 446, 568, 698, 715, 763
CviKI_1 RGCY 7 cut(s) 311, 335, 446, 568, 698, 715, 763
CviQI GTAC 3 cut(s) 79, 359, 366
DdeI CTNAG 2 cut(s) 144, 552
DpnI GATC 3 cut(s) 258, 489, 741
DpnII GATC 3 cut(s) 256, 487, 739
DriI GACNNNNNGTC 2 cut(s) 547, 832
Eam1104I CTCTTC 2 cut(s) 50, 63
Eam1105I GACNNNNNGTC 2 cut(s) 547, 832
EarI CTCTTC 2 cut(s) 50, 63
Eco105I TACGTA 1 cut(s) 358
Eco130I CCWWGG 2 cut(s) 174, 187
Eco31I GGTCTC 2 cut(s) 185, 690
Eco47I GGWCC 2 cut(s) 94, 826
Eco47III AGCGCT 1 cut(s) 42
Eco72I CACGTG 1 cut(s) 496
EcoRII CCWGG 3 cut(s) 50, 105, 753
EcoT14I CCWWGG 2 cut(s) 174, 187
ErhI CCWWGG 2 cut(s) 174, 187
FaeI CATG 3 cut(s) 374, 635, 815
FalI AAGNNNNNCTT 2 cut(s) 378, 410
FatI CATG 3 cut(s) 370, 631, 811
FauNDI CATATG 1 cut(s) 847
Fnu4HI GCNGC 2 cut(s) 309, 566
FokI GGATG 1 cut(s) 462
Fsp4HI GCNGC 2 cut(s) 309, 566
FspBI CTAG 1 cut(s) 188
GlaI GCGC 1 cut(s) 42
GluI GCNGC 2 cut(s) 309, 566
GsuI CTGGAG 1 cut(s) 146
HaeII RGCGCY 1 cut(s) 44
HapII CCGG 1 cut(s) 267
HgaI GACGC 1 cut(s) 571
HhaI GCGC 1 cut(s) 43
Hin1II CATG 3 cut(s) 374, 635, 815
Hin6I GCGC 1 cut(s) 41
HinP1I GCGC 1 cut(s) 41
HinfI GANTC 5 cut(s) 112, 327, 515, 601, 791
HpaII CCGG 1 cut(s) 267
HphI GGTGA 2 cut(s) 121, 653
Hpy166II GTNNAC 1 cut(s) 355
Hpy188I TCNGA 8 cut(s) 102, 226, 405, 520, 553, 561, 600, 606
Hpy188III TCNNGA 2 cut(s) 116, 666
Hpy8I GTNNAC 1 cut(s) 355
Hpy99I CGWCG 1 cut(s) 409
HpyAV CCTTC 5 cut(s) 58, 127, 603, 603, 817
HpyCH4III ACNGT 1 cut(s) 280
HpyCH4IV ACGT 4 cut(s) 357, 390, 407, 495
HpyCH4V TGCA 3 cut(s) 161, 308, 815
HpyF10VI GCNNNNNNNGC 1 cut(s) 452
HpyF3I CTNAG 2 cut(s) 144, 552
HpySE526I ACGT 4 cut(s) 357, 390, 407, 495
Hsp92II CATG 3 cut(s) 374, 635, 815
HspAI GCGC 1 cut(s) 41
Kzo9I GATC 3 cut(s) 256, 487, 739
LguI GCTCTTC 1 cut(s) 50
LmnI GCTCC 3 cut(s) 127, 460, 573
Lsp1109I GCAGC 1 cut(s) 320
MabI ACCWGGT 1 cut(s) 753
MaeI CTAG 1 cut(s) 188
MaeII ACGT 4 cut(s) 357, 390, 407, 495
MaeIII GTNAC 2 cut(s) 215, 641
MalI GATC 3 cut(s) 258, 489, 741
MbiI CCGCTC 1 cut(s) 130
MboI GATC 3 cut(s) 256, 487, 739
MboII GAAGA 9 cut(s) 37, 80, 398, 539, 542, 581, 681, 734, 800
MflI RGATCY 1 cut(s) 256
MluCI AATT 4 cut(s) 86, 235, 243, 672
MlyI GAGTC 1 cut(s) 336
MmeI TCCRAC 3 cut(s) 129, 428, 595
MnlI CCTC 8 cut(s) 52, 130, 242, 271, 436, 468, 510, 665
MseI TTAA 2 cut(s) 582, 675
MslI CAYNNNNRTG 2 cut(s) 636, 777
MspI CCGG 1 cut(s) 267
MspR9I CCNGG 3 cut(s) 52, 107, 755
Mva1269I GAATGC 1 cut(s) 310
MvaI CCWGG 3 cut(s) 52, 107, 755
MvnI CGCG 1 cut(s) 565
MwoI GCNNNNNNNGC 1 cut(s) 452
NdeI CATATG 1 cut(s) 847
NdeII GATC 3 cut(s) 256, 487, 739
NlaIII CATG 3 cut(s) 374, 635, 815
NlaIV GGNNCC 3 cut(s) 141, 258, 569
NmuCI GTSAC 2 cut(s) 215, 641
PasI CCCWGGG 1 cut(s) 51
PciSI GCTCTTC 1 cut(s) 50
PcsI WCGNNNNNNNCGW 1 cut(s) 417
PctI GAATGC 1 cut(s) 310
PfeI GAWTC 4 cut(s) 112, 515, 601, 791
PkrI GCNGC 2 cut(s) 310, 567
PleI GAGTC 1 cut(s) 335
PmaCI CACGTG 1 cut(s) 496
PmlI CACGTG 1 cut(s) 496
PpsI GAGTC 1 cut(s) 335
Ppu21I YACGTR 2 cut(s) 358, 496
Psp6I CCWGG 3 cut(s) 50, 105, 753
PspCI CACGTG 1 cut(s) 496
PspGI CCWGG 3 cut(s) 50, 105, 753
PspN4I GGNNCC 3 cut(s) 141, 258, 569
PspPI GGNCC 2 cut(s) 94, 826
PsuI RGATCY 1 cut(s) 256
RsaI GTAC 3 cut(s) 80, 360, 367
RsaNI GTAC 3 cut(s) 79, 359, 366
RseI CAYNNNNRTG 2 cut(s) 636, 777
SapI GCTCTTC 1 cut(s) 50
SaqAI TTAA 2 cut(s) 582, 675
SatI GCNGC 2 cut(s) 309, 566
Sau3AI GATC 3 cut(s) 256, 487, 739
Sau96I GGNCC 2 cut(s) 94, 826
SchI GAGTC 1 cut(s) 336
ScrFI CCNGG 3 cut(s) 52, 107, 755
SexAI ACCWGGT 1 cut(s) 753
SinI GGWCC 2 cut(s) 94, 826
SmiMI CAYNNNNRTG 2 cut(s) 636, 777
SnaBI TACGTA 1 cut(s) 358
Sse9I AATT 4 cut(s) 86, 235, 243, 672
SsiI CCGC 3 cut(s) 130, 565, 801
SspMI CTAG 1 cut(s) 188
StyD4I CCNGG 3 cut(s) 50, 105, 753
StyI CCWWGG 2 cut(s) 174, 187
TaaI ACNGT 1 cut(s) 280
TaiI ACGT 4 cut(s) 360, 393, 410, 498
TaqI TCGA 3 cut(s) 411, 420, 490
TaqII GACCGA 2 cut(s) 672, 678
TasI AATT 4 cut(s) 86, 235, 243, 672
TatI WGTACW 1 cut(s) 78
TauI GCSGC 1 cut(s) 568
TfiI GAWTC 4 cut(s) 112, 515, 601, 791
Tru1I TTAA 2 cut(s) 582, 675
Tru9I TTAA 2 cut(s) 582, 675
TscAI CASTG 1 cut(s) 283
TseFI GTSAC 2 cut(s) 215, 641
TseI GCWGC 1 cut(s) 308
Tsp45I GTSAC 2 cut(s) 215, 641
TspDTI ATGAA 5 cut(s) 399, 486, 540, 620, 800
TspGWI ACGGA 1 cut(s) 189
TspRI CASTG 1 cut(s) 283
VpaK11BI GGWCC 2 cut(s) 94, 826
XapI RAATTY 1 cut(s) 243
XmaJI CCTAGG 1 cut(s) 187
XspI CTAG 1 cut(s) 188
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.