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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccIII | TCCGGA | 1 cut(s) | 49 |
| AclWI | GGATC | 1 cut(s) | 60 |
| AgsI | TTSAA | 1 cut(s) | 11 |
| AluBI | AGCT | 1 cut(s) | 16 |
| AluI | AGCT | 1 cut(s) | 16 |
| AlwI | GGATC | 1 cut(s) | 60 |
| Aor13HI | TCCGGA | 1 cut(s) | 49 |
| ApeKI | GCWGC | 1 cut(s) | 16 |
| AseI | ATTAAT | 1 cut(s) | 183 |
| BbvI | GCAGC | 1 cut(s) | 3 |
| BisI | GCNGC | 1 cut(s) | 17 |
| BlsI | GCNGC | 1 cut(s) | 18 |
| BmsI | GCATC | 1 cut(s) | 34 |
| BpmI | CTGGAG | 1 cut(s) | 5 |
| BsaWI | WCCGGW | 1 cut(s) | 49 |
| BsaXI | ACNNNNNCTCC | 1 cut(s) | 33 |
| BseAI | TCCGGA | 1 cut(s) | 49 |
| BseGI | GGATG | 1 cut(s) | 94 |
| BseRI | GAGGAG | 1 cut(s) | 36 |
| BseXI | GCAGC | 1 cut(s) | 3 |
| BsiSI | CCGG | 1 cut(s) | 50 |
| Bsp13I | TCCGGA | 1 cut(s) | 49 |
| Bsp143I | GATC | 1 cut(s) | 52 |
| BspEI | TCCGGA | 1 cut(s) | 49 |
| BspPI | GGATC | 1 cut(s) | 60 |
| BssMI | GATC | 1 cut(s) | 52 |
| BstDEI | CTNAG | 1 cut(s) | 111 |
| BstF5I | GGATG | 1 cut(s) | 94 |
| BstKTI | GATC | 1 cut(s) | 55 |
| BstMBI | GATC | 1 cut(s) | 52 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 22 |
| BstV1I | GCAGC | 1 cut(s) | 3 |
| BtsCI | GGATG | 1 cut(s) | 94 |
| CviAII | CATG | 2 cut(s) | 140, 161 |
| CviJI | RGCY | 2 cut(s) | 16, 121 |
| CviKI_1 | RGCY | 2 cut(s) | 16, 121 |
| DdeI | CTNAG | 1 cut(s) | 111 |
| DpnI | GATC | 1 cut(s) | 54 |
| DpnII | GATC | 1 cut(s) | 52 |
| FaeI | CATG | 2 cut(s) | 143, 164 |
| FaiI | YATR | 5 cut(s) | 30, 84, 102, 141, 162 |
| FatI | CATG | 2 cut(s) | 139, 160 |
| Fnu4HI | GCNGC | 1 cut(s) | 17 |
| FokI | GGATG | 1 cut(s) | 101 |
| Fsp4HI | GCNGC | 1 cut(s) | 17 |
| GluI | GCNGC | 1 cut(s) | 17 |
| GsuI | CTGGAG | 1 cut(s) | 5 |
| HapII | CCGG | 1 cut(s) | 50 |
| Hin1II | CATG | 2 cut(s) | 143, 164 |
| HpaII | CCGG | 1 cut(s) | 50 |
| Hpy166II | GTNNAC | 1 cut(s) | 137 |
| Hpy188I | TCNGA | 1 cut(s) | 57 |
| Hpy188III | TCNNGA | 1 cut(s) | 50 |
| Hpy8I | GTNNAC | 1 cut(s) | 137 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 22 |
| HpyF3I | CTNAG | 1 cut(s) | 111 |
| Hsp92II | CATG | 2 cut(s) | 143, 164 |
| Kpn2I | TCCGGA | 1 cut(s) | 49 |
| Kzo9I | GATC | 1 cut(s) | 52 |
| LmnI | GCTCC | 1 cut(s) | 24 |
| LpnPI | CCDG | 2 cut(s) | 35, 63 |
| Lsp1109I | GCAGC | 1 cut(s) | 3 |
| LweI | GCATC | 1 cut(s) | 34 |
| MaeIII | GTNAC | 1 cut(s) | 106 |
| MalI | GATC | 1 cut(s) | 54 |
| MboI | GATC | 1 cut(s) | 52 |
| MboII | GAAGA | 1 cut(s) | 32 |
| MluCI | AATT | 1 cut(s) | 177 |
| MnlI | CCTC | 3 cut(s) | 54, 57, 136 |
| MroI | TCCGGA | 1 cut(s) | 49 |
| MseI | TTAA | 1 cut(s) | 183 |
| MslI | CAYNNNNRTG | 1 cut(s) | 30 |
| MspA1I | CMGCKG | 1 cut(s) | 16 |
| MspI | CCGG | 1 cut(s) | 50 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 22 |
| NdeII | GATC | 1 cut(s) | 52 |
| NlaIII | CATG | 2 cut(s) | 143, 164 |
| NmuCI | GTSAC | 1 cut(s) | 106 |
| PkrI | GCNGC | 1 cut(s) | 18 |
| PshBI | ATTAAT | 1 cut(s) | 183 |
| PvuII | CAGCTG | 1 cut(s) | 16 |
| RseI | CAYNNNNRTG | 1 cut(s) | 30 |
| SaqAI | TTAA | 1 cut(s) | 183 |
| SatI | GCNGC | 1 cut(s) | 17 |
| Sau3AI | GATC | 1 cut(s) | 52 |
| SetI | ASST | 2 cut(s) | 18, 65 |
| SfaNI | GCATC | 1 cut(s) | 34 |
| SgeI | CNNG | 7 cut(s) | 34, 62, 109, 142, 152, 166, 173 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 30 |
| Sse9I | AATT | 1 cut(s) | 177 |
| TasI | AATT | 1 cut(s) | 177 |
| Tru1I | TTAA | 1 cut(s) | 183 |
| Tru9I | TTAA | 1 cut(s) | 183 |
| TseFI | GTSAC | 1 cut(s) | 106 |
| TseI | GCWGC | 1 cut(s) | 16 |
| Tsp45I | GTSAC | 1 cut(s) | 106 |
| TspDTI | ATGAA | 1 cut(s) | 26 |
| VspI | ATTAAT | 1 cut(s) | 183 |