Rh2BG412000

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0001040
Physical Location & Seq
Reverse (-)
58301959 .. 58304684
2726 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG412000.1

Sequence Viewer

Length: 948 bp
ATGGGGGAGAAGGTCGAAAACTATTTGTACTTGCAATTTAAGGGACATCTTAGAAGGGTGAATCTTAACAAGCTCGAGGAGCAAAAGGAATCTCCTGAGTTTGAACTAGTGGCAGATTTTCTGGAGGAGAAACTCCCTTATGGAATGTGTGTTTTTTTGCAAGATTCGAATAAATTGTACATGGTTGGAGGAGAAAGGATTCGGAAGAATGAAACAGCTGGAAGGTATTGGTGGCAAGGATTACCCGTTGCTGCAAACGAATTTGACCCCTCTGGCCTCACTGGTGATGTTTTTGTGTTAGATTCCTCCAACGGAAAAATCTCTGCTGCTCCTGGTTTTCCAAAACCAAAAGCACCAAAGATCAATGCCAAAGCTTTGGCCACCATCGGTGACAAGGTATACGTAATGAGTTTTCGGCCATATTACTATCGAGGTACGCTTCCATCTCCGGCCTTTGAGTGCTATGATTCTGCAAAACAGTCATGGGAGGAGCTGCCAGTGCCTCCAAAATACTATCATTTGTGTTGTGCTTCTCCTACTCATGGGTTTCATTTTGTGCTGGGCCCAAAGATTTATTTGTGCACTCGGCATGGGTTCTTTTGTTACAACGTGGATCAGTCCCCACCTAAATGGGAATTCATAGTAGATGCAAAGTGGCATTATGATCGGGACACCTCATATGAAACAGAAGTGGAAATGGTAGACCACAAGATTGAGGACTCCTCACTGCCTCTGATACCGGCTTGGCCCTACCATTCTATATGTTTTGCAGAACCGGGAAAAGGGGGCCTCGCCGTTGGCTATACTGCTCAAGGATTAGTGGGTTACTTCTTTGCGCCTAATGGCGACCTTCTGTATCAAGAGCCACTTAATCAGTTGAACAGCAACTCTAAGCTTCCCTCCCCCGATAACTATTTCTTTGGTGGAAAAGTTTGCTATGTTGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

315

Amino Acids

35.79

Weight (kDa)

5.67

Isoelectric Point (pI)

50.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Kelch_FKB95 PF25210 94 - 213 3.5e-06 FKB95, Kelch-repeats domain
DUF1668 PF07893 95 - 212 2.1e-06 Protein of unknown function (DUF1668)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 399, 702
AclWI GGATC 1 cut(s) 621
AcoI YGGCCR 2 cut(s) 378, 416
AcsI RAATTY 2 cut(s) 260, 635
AfaI GTAC 3 cut(s) 29, 179, 436
AfiI CCNNNNNNNGG 2 cut(s) 542, 782
AgsI TTSAA 2 cut(s) 104, 880
AhlI ACTAGT 1 cut(s) 106
AjnI CCWGG 1 cut(s) 331
AluBI AGCT 5 cut(s) 73, 218, 374, 493, 895
AluI AGCT 5 cut(s) 73, 218, 374, 493, 895
Alw21I GWGCWC 1 cut(s) 584
Alw44I GTGCAC 1 cut(s) 580
AlwI GGATC 1 cut(s) 621
Ama87I CYCGRG 1 cut(s) 74
AoxI GGCC 7 cut(s) 274, 378, 416, 450, 562, 746, 787
ApaI GGGCCC 1 cut(s) 566
ApaLI GTGCAC 1 cut(s) 580
ApeKI GCWGC 3 cut(s) 251, 326, 493
ApoI RAATTY 2 cut(s) 260, 635
Asp700I GAANNNNTTC 1 cut(s) 198
AspLEI GCGC 1 cut(s) 838
AspS9I GGNCC 4 cut(s) 562, 563, 747, 787
AsuC2I CCSGG 1 cut(s) 777
AsuHPI GGTGA 3 cut(s) 70, 296, 401
AsuII TTCGAA 1 cut(s) 167
AvaI CYCGRG 1 cut(s) 74
BaeGI GKGCMC 2 cut(s) 566, 584
BalI TGGCCA 1 cut(s) 380
BanII GRGCYC 1 cut(s) 566
Bbv12I GWGCWC 1 cut(s) 584
BbvI GCAGC 3 cut(s) 238, 313, 480
BccI CCATC 2 cut(s) 392, 451
BceAI ACGGC 1 cut(s) 779
BcgI CGANNNNNNTGC 2 cut(s) 647, 681
BciT130I CCWGG 1 cut(s) 333
BcnI CCSGG 1 cut(s) 777
BcuI ACTAGT 1 cut(s) 106
BfaI CTAG 1 cut(s) 107
BisI GCNGC 3 cut(s) 252, 327, 494
BlsI GCNGC 3 cut(s) 253, 328, 495
Bme1390I CCNGG 2 cut(s) 333, 777
BmeT110I CYCGRG 1 cut(s) 74
BmgT120I GGNCC 4 cut(s) 562, 563, 747, 787
BmiI GGNNCC 2 cut(s) 564, 788
BmrFI CCNGG 2 cut(s) 333, 777
BmsI GCATC 1 cut(s) 637
BplI GAGNNNNNCTC 2 cut(s) 707, 739
BpmI CTGGAG 1 cut(s) 143
Bpu14I TTCGAA 1 cut(s) 167
BpuEI CTTGAG 1 cut(s) 795
BpuMI CCSGG 1 cut(s) 777
BsaAI YACGTR 1 cut(s) 403
Bsc4I CCNNNNNNNGG 2 cut(s) 542, 782
Bse118I RCCGGY 1 cut(s) 739
Bse1I ACTGG 2 cut(s) 286, 497
BseBI CCWGG 1 cut(s) 333
BseLI CCNNNNNNNGG 2 cut(s) 542, 782
BseMII CTCAG 1 cut(s) 87
BseNI ACTGG 2 cut(s) 286, 497
BseRI GAGGAG 5 cut(s) 92, 140, 204, 503, 712
BseSI GKGCMC 2 cut(s) 566, 584
BseXI GCAGC 3 cut(s) 238, 313, 480
BseYI CCCAGC 1 cut(s) 559
BshFI GGCC 7 cut(s) 276, 380, 418, 452, 564, 748, 789
BsiHKAI GWGCWC 1 cut(s) 584
BsiHKCI CYCGRG 1 cut(s) 74
BsiSI CCGG 3 cut(s) 449, 740, 776
BslFI GGGAC 3 cut(s) 57, 604, 683
BslI CCNNNNNNNGG 2 cut(s) 542, 782
BsmFI GGGAC 3 cut(s) 57, 604, 683
BsnI GGCC 7 cut(s) 276, 380, 418, 452, 564, 748, 789
BsoBI CYCGRG 1 cut(s) 74
Bsp119I TTCGAA 1 cut(s) 167
Bsp120I GGGCCC 1 cut(s) 562
Bsp1286I GDGCHC 2 cut(s) 566, 584
Bsp1407I TGTACA 1 cut(s) 177
Bsp143I GATC 3 cut(s) 360, 613, 664
BspANI GGCC 7 cut(s) 276, 380, 418, 452, 564, 748, 789
BspCNI CTCAG 1 cut(s) 88
BspLI GGNNCC 2 cut(s) 564, 788
BspPI GGATC 1 cut(s) 621
BspT104I TTCGAA 1 cut(s) 167
BsrFI RCCGGY 1 cut(s) 739
BsrGI TGTACA 1 cut(s) 177
BsrI ACTGG 2 cut(s) 286, 497
BssAI RCCGGY 1 cut(s) 739
BssMI GATC 3 cut(s) 360, 613, 664
BssNAI GTATAC 1 cut(s) 400
Bst1107I GTATAC 1 cut(s) 400
Bst2UI CCWGG 1 cut(s) 333
Bst4CI ACNGT 1 cut(s) 480
BstAUI TGTACA 1 cut(s) 177
BstBAI YACGTR 1 cut(s) 403
BstBI TTCGAA 1 cut(s) 167
BstDEI CTNAG 3 cut(s) 50, 96, 891
BstHHI GCGC 1 cut(s) 838
BstKTI GATC 3 cut(s) 363, 616, 667
BstMBI GATC 3 cut(s) 360, 613, 664
BstMWI GCNNNNNNNGC 2 cut(s) 79, 499
BstNI CCWGG 1 cut(s) 333
BstSCI CCNGG 2 cut(s) 331, 775
BstSLI GKGCMC 2 cut(s) 566, 584
BstSNI TACGTA 1 cut(s) 403
BstV1I GCAGC 3 cut(s) 238, 313, 480
BstXI CCANNNNNNTGG 2 cut(s) 376, 630
BstZ17I GTATAC 1 cut(s) 400
BsuRI GGCC 7 cut(s) 276, 380, 418, 452, 564, 748, 789
BtsI GCAGTG 1 cut(s) 725
BtsIMutI CAGTG 3 cut(s) 279, 504, 725
CfoI GCGC 1 cut(s) 838
Cfr10I RCCGGY 1 cut(s) 739
Cfr13I GGNCC 4 cut(s) 562, 563, 747, 787
Csp6I GTAC 3 cut(s) 28, 178, 435
CviAII CATG 4 cut(s) 181, 483, 542, 590
CviQI GTAC 3 cut(s) 28, 178, 435
DdeI CTNAG 3 cut(s) 50, 96, 891
DpnI GATC 3 cut(s) 362, 615, 666
DpnII GATC 3 cut(s) 360, 613, 664
EaeI YGGCCR 2 cut(s) 378, 416
Eco105I TACGTA 1 cut(s) 403
Eco24I GRGCYC 1 cut(s) 566
Eco88I CYCGRG 1 cut(s) 74
EcoO109I RGGNCCY 1 cut(s) 787
EcoRI GAATTC 1 cut(s) 635
EcoRII CCWGG 1 cut(s) 331
EcoT38I GRGCYC 1 cut(s) 566
FaeI CATG 4 cut(s) 184, 486, 545, 593
FalI AAGNNNNNCTT 2 cut(s) 852, 884
FaqI GGGAC 3 cut(s) 57, 604, 683
FatI CATG 4 cut(s) 180, 482, 541, 589
FauNDI CATATG 1 cut(s) 679
FblI GTMKAC 2 cut(s) 399, 702
Fnu4HI GCNGC 3 cut(s) 252, 327, 494
FriOI GRGCYC 1 cut(s) 566
Fsp4HI GCNGC 3 cut(s) 252, 327, 494
FspBI CTAG 1 cut(s) 107
GlaI GCGC 1 cut(s) 837
GluI GCNGC 3 cut(s) 252, 327, 494
GsaI CCCAGC 1 cut(s) 563
GsuI CTGGAG 1 cut(s) 143
HaeIII GGCC 7 cut(s) 276, 380, 418, 452, 564, 748, 789
HapII CCGG 3 cut(s) 449, 740, 776
HhaI GCGC 1 cut(s) 838
Hin1II CATG 4 cut(s) 184, 486, 545, 593
Hin6I GCGC 1 cut(s) 836
HinP1I GCGC 1 cut(s) 836
HindIII AAGCTT 2 cut(s) 372, 893
HinfI GANTC 7 cut(s) 61, 89, 164, 199, 302, 467, 719
HpaII CCGG 3 cut(s) 449, 740, 776
HphI GGTGA 3 cut(s) 70, 296, 401
Hpy166II GTNNAC 3 cut(s) 400, 582, 703
Hpy188I TCNGA 2 cut(s) 204, 735
Hpy188III TCNNGA 4 cut(s) 95, 122, 668, 860
Hpy8I GTNNAC 3 cut(s) 400, 582, 703
HpyAV CCTTC 4 cut(s) 4, 48, 216, 860
HpyCH4III ACNGT 1 cut(s) 480
HpyCH4IV ACGT 2 cut(s) 402, 609
HpyCH4V TGCA 7 cut(s) 34, 160, 254, 473, 582, 650, 770
HpyF10VI GCNNNNNNNGC 2 cut(s) 79, 499
HpyF3I CTNAG 3 cut(s) 50, 96, 891
HpySE526I ACGT 2 cut(s) 402, 609
Hsp92II CATG 4 cut(s) 184, 486, 545, 593
HspAI GCGC 1 cut(s) 836
Kzo9I GATC 3 cut(s) 360, 613, 664
LmnI GCTCC 3 cut(s) 79, 334, 490
Lsp1109I GCAGC 3 cut(s) 238, 313, 480
LweI GCATC 1 cut(s) 637
MaeI CTAG 1 cut(s) 107
MaeII ACGT 2 cut(s) 402, 609
MaeIII GTNAC 3 cut(s) 389, 602, 824
MalI GATC 3 cut(s) 362, 615, 666
MboI GATC 3 cut(s) 360, 613, 664
MboII GAAGA 1 cut(s) 217
MhlI GDGCHC 2 cut(s) 566, 584
MlsI TGGCCA 1 cut(s) 380
MluCI AATT 4 cut(s) 35, 173, 260, 635
MluNI TGGCCA 1 cut(s) 380
MlyI GAGTC 1 cut(s) 713
MmeI TCCRAC 2 cut(s) 166, 333
Mox20I TGGCCA 1 cut(s) 380
MroXI GAANNNNTTC 1 cut(s) 198
MscI TGGCCA 1 cut(s) 380
MseI TTAA 3 cut(s) 39, 66, 870
MslI CAYNNNNRTG 1 cut(s) 628
Msp20I TGGCCA 1 cut(s) 380
MspA1I CMGCKG 1 cut(s) 218
MspI CCGG 3 cut(s) 449, 740, 776
MspR9I CCNGG 2 cut(s) 333, 777
MvaI CCWGG 1 cut(s) 333
MwoI GCNNNNNNNGC 2 cut(s) 79, 499
NciI CCSGG 1 cut(s) 777
NdeI CATATG 1 cut(s) 679
NdeII GATC 3 cut(s) 360, 613, 664
NlaIII CATG 4 cut(s) 184, 486, 545, 593
NlaIV GGNNCC 2 cut(s) 564, 788
NmeAIII GCCGAG 1 cut(s) 565
NmuCI GTSAC 1 cut(s) 389
NspV TTCGAA 1 cut(s) 167
PaeR7I CTCGAG 1 cut(s) 74
PdmI GAANNNNTTC 1 cut(s) 198
PfeI GAWTC 6 cut(s) 61, 89, 164, 199, 302, 467
PkrI GCNGC 3 cut(s) 253, 328, 495
PleI GAGTC 1 cut(s) 713
PpsI GAGTC 1 cut(s) 713
Ppu21I YACGTR 1 cut(s) 403
Psp6I CCWGG 1 cut(s) 331
PspFI CCCAGC 1 cut(s) 559
PspGI CCWGG 1 cut(s) 331
PspN4I GGNNCC 2 cut(s) 564, 788
PspOMI GGGCCC 1 cut(s) 562
PspPI GGNCC 4 cut(s) 562, 563, 747, 787
PspXI VCTCGAGB 1 cut(s) 74
PvuII CAGCTG 1 cut(s) 218
RsaI GTAC 3 cut(s) 29, 179, 436
RsaNI GTAC 3 cut(s) 28, 178, 435
RseI CAYNNNNRTG 1 cut(s) 628
SaqAI TTAA 3 cut(s) 39, 66, 870
SatI GCNGC 3 cut(s) 252, 327, 494
Sau3AI GATC 3 cut(s) 360, 613, 664
Sau96I GGNCC 4 cut(s) 562, 563, 747, 787
SchI GAGTC 1 cut(s) 713
ScrFI CCNGG 2 cut(s) 333, 777
SduI GDGCHC 2 cut(s) 566, 584
SfaNI GCATC 1 cut(s) 637
Sfr274I CTCGAG 1 cut(s) 74
SfuI TTCGAA 1 cut(s) 167
SlaI CTCGAG 1 cut(s) 74
SmiMI CAYNNNNRTG 1 cut(s) 628
SmlI CTYRAG 2 cut(s) 74, 810
SmoI CTYRAG 2 cut(s) 74, 810
SnaBI TACGTA 1 cut(s) 403
SpeI ACTAGT 1 cut(s) 106
Sse9I AATT 4 cut(s) 35, 173, 260, 635
SspMI CTAG 1 cut(s) 107
StyD4I CCNGG 2 cut(s) 331, 775
TaaI ACNGT 1 cut(s) 480
TaiI ACGT 2 cut(s) 405, 612
TaqI TCGA 4 cut(s) 15, 75, 167, 430
TasI AATT 4 cut(s) 35, 173, 260, 635
TatI WGTACW 2 cut(s) 27, 177
TfiI GAWTC 6 cut(s) 61, 89, 164, 199, 302, 467
Tru1I TTAA 3 cut(s) 39, 66, 870
Tru9I TTAA 3 cut(s) 39, 66, 870
TscAI CASTG 3 cut(s) 286, 504, 732
TseFI GTSAC 1 cut(s) 389
TseI GCWGC 3 cut(s) 251, 326, 493
Tsp45I GTSAC 1 cut(s) 389
TspDTI ATGAA 4 cut(s) 225, 539, 628, 696
TspGWI ACGGA 1 cut(s) 327
TspRI CASTG 3 cut(s) 286, 504, 732
VneI GTGCAC 1 cut(s) 580
XapI RAATTY 2 cut(s) 260, 635
XhoI CTCGAG 1 cut(s) 74
XmiI GTMKAC 2 cut(s) 399, 702
XmnI GAANNNNTTC 1 cut(s) 198
XspI CTAG 1 cut(s) 107
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.