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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AciI | CCGC | 2 cut(s) | 140, 254 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 101 |
| AgsI | TTSAA | 2 cut(s) | 46, 179 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 304 |
| AjnI | CCWGG | 1 cut(s) | 94 |
| AluBI | AGCT | 1 cut(s) | 169 |
| AluI | AGCT | 1 cut(s) | 169 |
| AoxI | GGCC | 2 cut(s) | 65, 231 |
| ApeKI | GCWGC | 2 cut(s) | 169, 261 |
| AspS9I | GGNCC | 1 cut(s) | 66 |
| BbsI | GAAGAC | 1 cut(s) | 45 |
| BbvI | GCAGC | 2 cut(s) | 156, 273 |
| BccI | CCATC | 1 cut(s) | 155 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 44, 78 |
| BciT130I | CCWGG | 1 cut(s) | 96 |
| BclI | TGATCA | 1 cut(s) | 198 |
| BfmI | CTRYAG | 1 cut(s) | 170 |
| BisI | GCNGC | 2 cut(s) | 170, 262 |
| BlsI | GCNGC | 2 cut(s) | 171, 263 |
| Bme1390I | CCNGG | 1 cut(s) | 96 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 304 |
| BmgT120I | GGNCC | 1 cut(s) | 66 |
| BmrFI | CCNGG | 1 cut(s) | 96 |
| BpiI | GAAGAC | 1 cut(s) | 45 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 101 |
| BseBI | CCWGG | 1 cut(s) | 96 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 101 |
| BseXI | GCAGC | 2 cut(s) | 156, 273 |
| BseYI | CCCAGC | 1 cut(s) | 301 |
| BshFI | GGCC | 2 cut(s) | 67, 233 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 101 |
| BsnI | GGCC | 2 cut(s) | 67, 233 |
| Bsp143I | GATC | 2 cut(s) | 198, 211 |
| BspACI | CCGC | 2 cut(s) | 140, 254 |
| BspANI | GGCC | 2 cut(s) | 67, 233 |
| BspMAI | CTGCAG | 1 cut(s) | 174 |
| BssMI | GATC | 2 cut(s) | 198, 211 |
| Bst2UI | CCWGG | 1 cut(s) | 96 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 270 |
| BstC8I | GCNNGC | 1 cut(s) | 167 |
| BstKTI | GATC | 2 cut(s) | 201, 214 |
| BstMBI | GATC | 2 cut(s) | 198, 211 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 230, 270 |
| BstNI | CCWGG | 1 cut(s) | 96 |
| BstSCI | CCNGG | 1 cut(s) | 94 |
| BstSFI | CTRYAG | 1 cut(s) | 170 |
| BstV1I | GCAGC | 2 cut(s) | 156, 273 |
| BstV2I | GAAGAC | 1 cut(s) | 45 |
| BstXI | CCANNNNNNTGG | 2 cut(s) | 257, 309 |
| BsuRI | GGCC | 2 cut(s) | 67, 233 |
| Cac8I | GCNNGC | 1 cut(s) | 167 |
| Cfr13I | GGNCC | 1 cut(s) | 66 |
| CseI | GACGC | 1 cut(s) | 294 |
| CsiI | ACCWGGT | 1 cut(s) | 94 |
| CviAII | CATG | 2 cut(s) | 251, 309 |
| CviJI | RGCY | 7 cut(s) | 49, 67, 108, 120, 169, 184, 233 |
| CviKI_1 | RGCY | 7 cut(s) | 49, 67, 108, 120, 169, 184, 233 |
| DpnI | GATC | 2 cut(s) | 200, 213 |
| DpnII | GATC | 2 cut(s) | 198, 211 |
| DriI | GACNNNNNGTC | 1 cut(s) | 304 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 304 |
| EcoRII | CCWGG | 1 cut(s) | 94 |
| FaeI | CATG | 2 cut(s) | 254, 312 |
| FaiI | YATR | 6 cut(s) | 53, 123, 252, 285, 296, 310 |
| FatI | CATG | 2 cut(s) | 250, 308 |
| FauI | CCCGC | 2 cut(s) | 133, 247 |
| FbaI | TGATCA | 1 cut(s) | 198 |
| Fnu4HI | GCNGC | 2 cut(s) | 170, 262 |
| Fsp4HI | GCNGC | 2 cut(s) | 170, 262 |
| GluI | GCNGC | 2 cut(s) | 170, 262 |
| GsaI | CCCAGC | 1 cut(s) | 305 |
| HaeIII | GGCC | 2 cut(s) | 67, 233 |
| HgaI | GACGC | 1 cut(s) | 294 |
| Hin1II | CATG | 2 cut(s) | 254, 312 |
| Hpy188I | TCNGA | 2 cut(s) | 10, 211 |
| Hpy188III | TCNNGA | 1 cut(s) | 158 |
| HpyAV | CCTTC | 2 cut(s) | 26, 79 |
| HpyCH4V | TGCA | 2 cut(s) | 55, 172 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 230, 270 |
| Hsp92II | CATG | 2 cut(s) | 254, 312 |
| Ksp22I | TGATCA | 1 cut(s) | 198 |
| Kzo9I | GATC | 2 cut(s) | 198, 211 |
| LmnI | GCTCC | 1 cut(s) | 160 |
| LpnPI | CCDG | 5 cut(s) | 81, 108, 171, 215, 315 |
| Lsp1109I | GCAGC | 2 cut(s) | 156, 273 |
| MabI | ACCWGGT | 1 cut(s) | 94 |
| MaeIII | GTNAC | 1 cut(s) | 143 |
| MalI | GATC | 2 cut(s) | 200, 213 |
| MboI | GATC | 2 cut(s) | 198, 211 |
| MboII | GAAGA | 2 cut(s) | 50, 206 |
| MnlI | CCTC | 2 cut(s) | 22, 229 |
| MseI | TTAA | 1 cut(s) | 240 |
| MslI | CAYNNNNRTG | 1 cut(s) | 255 |
| MspR9I | CCNGG | 1 cut(s) | 96 |
| MvaI | CCWGG | 1 cut(s) | 96 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 230, 270 |
| NdeII | GATC | 2 cut(s) | 198, 211 |
| NlaIII | CATG | 2 cut(s) | 254, 312 |
| PkrI | GCNGC | 2 cut(s) | 171, 263 |
| Psp6I | CCWGG | 1 cut(s) | 94 |
| PspFI | CCCAGC | 1 cut(s) | 301 |
| PspGI | CCWGG | 1 cut(s) | 94 |
| PspPI | GGNCC | 1 cut(s) | 66 |
| PstI | CTGCAG | 1 cut(s) | 174 |
| RseI | CAYNNNNRTG | 1 cut(s) | 255 |
| SaqAI | TTAA | 1 cut(s) | 240 |
| SatI | GCNGC | 2 cut(s) | 170, 262 |
| Sau3AI | GATC | 2 cut(s) | 198, 211 |
| Sau96I | GGNCC | 1 cut(s) | 66 |
| ScrFI | CCNGG | 1 cut(s) | 96 |
| SetI | ASST | 3 cut(s) | 100, 171, 246 |
| SexAI | ACCWGGT | 1 cut(s) | 94 |
| SfcI | CTRYAG | 1 cut(s) | 170 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 255 |
| SsiI | CCGC | 2 cut(s) | 140, 254 |
| StyD4I | CCNGG | 1 cut(s) | 94 |
| TaqI | TCGA | 1 cut(s) | 21 |
| Tru1I | TTAA | 1 cut(s) | 240 |
| Tru9I | TTAA | 1 cut(s) | 240 |
| TseI | GCWGC | 2 cut(s) | 169, 261 |
| TspDTI | ATGAA | 1 cut(s) | 30 |
| TspGWI | ACGGA | 1 cut(s) | 67 |