Rh2DG423000

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0001040
Physical Location & Seq
Reverse (-)
61286869 .. 61288584
1716 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG423000.1

Sequence Viewer

Length: 1278 bp
ATGGGGGAGAAGGTCGAAAACTATTTGTACTTGCAATTTAAGGAACATCTTAGAAGGGTGAATCTTAACAAGCTCGAGGAGCAAAAGGAATCTCCTGAGTTTGAACTAGTGGCAGATTTTCTGGACGAGAAACTCCCCTATGGAATGTGTGTTTTTTTGCAAGATTCGAATAAATTGTACATGGTTGGAGGAGAAAGGATTCGGAAGAATGAAACAGCTGGAAGGTATTGGTGGCAAGGGTTACCCGTTGCTGCAAACGAATTAGACCCCTCTGGCCTCACTTGTGATGTTTTTGTGTTAGATTCCTCCAACGGAAAAATCTCTGCTGCTCCTGGTTTTCCAAAACCAAAAGGACCAAAGATCAATGCCAAAGCTTTGGCCACCATCGGTGACAAGGTATACGTAATGAGTTTTCGGCCATATTACTATCGAGGTACGCTTCCATCTCCGGCCTTTGAGTGCTATGATTCTGCAAAACAGTCATGGGAGGAGCTGCCTGTGCCTCCAAAATACTATCATTTGCGTTGTGCTTCTCCTACTCATGGGTTTCATTTTGTGCTGGGCCCAAAGATTTATTTGTGCACTCGTCATGGGTTCTTTTGTTACAACGTGGATCAGTCCCCACCTAAATGGGAATTCATAGTAGATGCAAAGTGGCATTATGATCGGGACACCTCATATGAAACAGAAGTGGAAATGGTAGACCACAAGATTGAGGACTCCTCACTGCCTCTGATACCGGCTTGGCCCTACCATTCTATATGTTTTGCAGAACCGGGAAAAGGAGGCCTCGCCGTTGGCTATACTGCCCAAGGATTAGTGGGTTACTTCTTTGCGCCTAATGGCGACCTACTGTATCAAGAGCCACTTAATCAGTTGAACAGCAACTCTAAGCTTCCCTCCCCCGATAACTATTTCTTTGGTGGAAAAGTTTGCTATGTAGGTGAAAACAAATTCTGTCTCATTCTATCCGGAGAAATTGATCAAACTTCATGGTCGTTGGCTGTAGCAACTTTTGATGTGAAATGGAAAGCGGATGTTCACAATGGTGATGGAGGAGGCTTCTACCATGATCTTCCTCTGTGTTATCACCAGTTTGACAATATTAATTCGTCTTTTTCTAATGCTTGCAAATATGAGAACAACGAGTCTCCGAGGCTTTATGATTACTTCAATTTCCAGGATGCCTTCGTCATATCCTCCGAGCCTAAGAGCCAGCGTCACCACAACCACAAGCGCAAGCGGAGTATGATGGGTGTAGGTTTTCAGTTGGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

425

Amino Acids

48.55

Weight (kDa)

5.98

Isoelectric Point (pI)

53.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Kelch_FKB95 PF25210 94 - 213 7.9e-06 FKB95, Kelch-repeats domain
DUF1668 PF07893 98 - 212 2.6e-06 Protein of unknown function (DUF1668)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 399, 702
AccIII TCCGGA 1 cut(s) 971
AciI CCGC 2 cut(s) 1034, 1243
AclWI GGATC 1 cut(s) 621
AcoI YGGCCR 2 cut(s) 378, 416
AcsI RAATTY 2 cut(s) 635, 953
AfaI GTAC 3 cut(s) 29, 179, 436
AfiI CCNNNNNNNGG 2 cut(s) 542, 782
AgsI TTSAA 3 cut(s) 104, 880, 1174
AhlI ACTAGT 1 cut(s) 106
AjnI CCWGG 2 cut(s) 331, 1179
AleI CACNNNNGTG 1 cut(s) 1047
AluBI AGCT 5 cut(s) 73, 218, 374, 493, 895
AluI AGCT 5 cut(s) 73, 218, 374, 493, 895
Alw21I GWGCWC 1 cut(s) 584
Alw26I GTCTC 2 cut(s) 965, 1155
Alw44I GTGCAC 1 cut(s) 580
AlwI GGATC 1 cut(s) 621
Ama87I CYCGRG 1 cut(s) 74
Aor13HI TCCGGA 1 cut(s) 971
AoxI GGCC 7 cut(s) 274, 378, 416, 450, 562, 746, 787
ApaI GGGCCC 1 cut(s) 566
ApaLI GTGCAC 1 cut(s) 580
ApeKI GCWGC 3 cut(s) 251, 326, 493
ApoI RAATTY 2 cut(s) 635, 953
AseI ATTAAT 1 cut(s) 1107
Asp700I GAANNNNTTC 1 cut(s) 198
AspLEI GCGC 2 cut(s) 838, 1239
AspS9I GGNCC 4 cut(s) 353, 562, 563, 747
AsuC2I CCSGG 1 cut(s) 777
AsuHPI GGTGA 6 cut(s) 70, 401, 956, 1061, 1082, 1214
AsuII TTCGAA 1 cut(s) 167
AvaI CYCGRG 1 cut(s) 74
AvaII GGWCC 1 cut(s) 353
BaeGI GKGCMC 2 cut(s) 566, 584
BalI TGGCCA 1 cut(s) 380
BanII GRGCYC 1 cut(s) 566
Bbv12I GWGCWC 1 cut(s) 584
BbvI GCAGC 3 cut(s) 238, 313, 480
BccI CCATC 4 cut(s) 392, 451, 1046, 1246
BceAI ACGGC 1 cut(s) 779
BcgI CGANNNNNNTGC 2 cut(s) 647, 681
BciT130I CCWGG 2 cut(s) 333, 1181
BclI TGATCA 1 cut(s) 982
BcnI CCSGG 1 cut(s) 777
BcoDI GTCTC 2 cut(s) 965, 1155
BcuI ACTAGT 1 cut(s) 106
BfaI CTAG 1 cut(s) 107
BfmI CTRYAG 1 cut(s) 1005
BisI GCNGC 3 cut(s) 252, 327, 494
BlsI GCNGC 3 cut(s) 253, 328, 495
Bme1390I CCNGG 3 cut(s) 333, 777, 1181
Bme18I GGWCC 1 cut(s) 353
BmeT110I CYCGRG 1 cut(s) 74
BmgT120I GGNCC 4 cut(s) 353, 562, 563, 747
BmiI GGNNCC 1 cut(s) 564
BmrFI CCNGG 3 cut(s) 333, 777, 1181
BmsI GCATC 2 cut(s) 637, 1174
BplI GAGNNNNNCTC 2 cut(s) 707, 739
Bpu14I TTCGAA 1 cut(s) 167
BpuMI CCSGG 1 cut(s) 777
BsaAI YACGTR 1 cut(s) 403
BsaJI CCNNGG 2 cut(s) 811, 1154
BsaWI WCCGGW 1 cut(s) 971
Bsc4I CCNNNNNNNGG 2 cut(s) 542, 782
Bse118I RCCGGY 1 cut(s) 739
Bse1I ACTGG 1 cut(s) 1093
BseAI TCCGGA 1 cut(s) 971
BseBI CCWGG 2 cut(s) 333, 1181
BseDI CCNNGG 2 cut(s) 811, 1154
BseGI GGATG 2 cut(s) 1042, 1189
BseLI CCNNNNNNNGG 2 cut(s) 542, 782
BseMII CTCAG 1 cut(s) 87
BseNI ACTGG 1 cut(s) 1093
BseRI GAGGAG 5 cut(s) 92, 204, 503, 712, 1071
BseSI GKGCMC 2 cut(s) 566, 584
BseXI GCAGC 3 cut(s) 238, 313, 480
BseYI CCCAGC 1 cut(s) 559
BshFI GGCC 7 cut(s) 276, 380, 418, 452, 564, 748, 789
BsiHKAI GWGCWC 1 cut(s) 584
BsiHKCI CYCGRG 1 cut(s) 74
BsiSI CCGG 4 cut(s) 449, 740, 776, 972
BslFI GGGAC 2 cut(s) 604, 683
BslI CCNNNNNNNGG 2 cut(s) 542, 782
BsmAI GTCTC 2 cut(s) 965, 1155
BsmFI GGGAC 2 cut(s) 604, 683
BsnI GGCC 7 cut(s) 276, 380, 418, 452, 564, 748, 789
BsoBI CYCGRG 1 cut(s) 74
Bsp119I TTCGAA 1 cut(s) 167
Bsp120I GGGCCC 1 cut(s) 562
Bsp1286I GDGCHC 2 cut(s) 566, 584
Bsp13I TCCGGA 1 cut(s) 971
Bsp1407I TGTACA 1 cut(s) 177
Bsp143I GATC 5 cut(s) 360, 613, 664, 982, 1072
BspACI CCGC 2 cut(s) 1034, 1243
BspANI GGCC 7 cut(s) 276, 380, 418, 452, 564, 748, 789
BspCNI CTCAG 1 cut(s) 88
BspEI TCCGGA 1 cut(s) 971
BspLI GGNNCC 1 cut(s) 564
BspPI GGATC 1 cut(s) 621
BspT104I TTCGAA 1 cut(s) 167
BsrFI RCCGGY 1 cut(s) 739
BsrGI TGTACA 1 cut(s) 177
BsrI ACTGG 1 cut(s) 1093
BssAI RCCGGY 1 cut(s) 739
BssECI CCNNGG 2 cut(s) 811, 1154
BssMI GATC 5 cut(s) 360, 613, 664, 982, 1072
BssNAI GTATAC 1 cut(s) 400
BssT1I CCWWGG 1 cut(s) 811
Bst1107I GTATAC 1 cut(s) 400
Bst2UI CCWGG 2 cut(s) 333, 1181
Bst4CI ACNGT 2 cut(s) 480, 855
BstAUI TGTACA 1 cut(s) 177
BstBAI YACGTR 1 cut(s) 403
BstBI TTCGAA 1 cut(s) 167
BstC8I GCNNGC 3 cut(s) 1129, 1217, 1241
BstDEI CTNAG 4 cut(s) 50, 96, 891, 1209
BstEII GGTNACC 1 cut(s) 240
BstF5I GGATG 2 cut(s) 1042, 1189
BstHHI GCGC 2 cut(s) 838, 1239
BstKTI GATC 5 cut(s) 363, 616, 667, 985, 1075
BstMAI GTCTC 2 cut(s) 965, 1155
BstMBI GATC 5 cut(s) 360, 613, 664, 982, 1072
BstMWI GCNNNNNNNGC 2 cut(s) 79, 499
BstNI CCWGG 2 cut(s) 333, 1181
BstPI GGTNACC 1 cut(s) 240
BstSCI CCNGG 3 cut(s) 331, 775, 1179
BstSFI CTRYAG 1 cut(s) 1005
BstSLI GKGCMC 2 cut(s) 566, 584
BstSNI TACGTA 1 cut(s) 403
BstV1I GCAGC 3 cut(s) 238, 313, 480
BstXI CCANNNNNNTGG 2 cut(s) 376, 630
BstZ17I GTATAC 1 cut(s) 400
BsuRI GGCC 7 cut(s) 276, 380, 418, 452, 564, 748, 789
BtsCI GGATG 2 cut(s) 1042, 1189
BtsI GCAGTG 1 cut(s) 725
BtsIMutI CAGTG 1 cut(s) 725
Cac8I GCNNGC 3 cut(s) 1129, 1217, 1241
CfoI GCGC 2 cut(s) 838, 1239
Cfr10I RCCGGY 1 cut(s) 739
Cfr13I GGNCC 4 cut(s) 353, 562, 563, 747
CseI GACGC 1 cut(s) 1208
Csp6I GTAC 3 cut(s) 28, 178, 435
CviAII CATG 6 cut(s) 181, 483, 542, 590, 993, 1070
CviQI GTAC 3 cut(s) 28, 178, 435
DdeI CTNAG 4 cut(s) 50, 96, 891, 1209
DpnI GATC 5 cut(s) 362, 615, 666, 984, 1074
DpnII GATC 5 cut(s) 360, 613, 664, 982, 1072
EaeI YGGCCR 2 cut(s) 378, 416
Eco105I TACGTA 1 cut(s) 403
Eco130I CCWWGG 1 cut(s) 811
Eco147I AGGCCT 1 cut(s) 789
Eco24I GRGCYC 1 cut(s) 566
Eco47I GGWCC 1 cut(s) 353
Eco88I CYCGRG 1 cut(s) 74
Eco91I GGTNACC 1 cut(s) 240
EcoO65I GGTNACC 1 cut(s) 240
EcoRI GAATTC 1 cut(s) 635
EcoRII CCWGG 2 cut(s) 331, 1179
EcoT14I CCWWGG 1 cut(s) 811
EcoT38I GRGCYC 1 cut(s) 566
ErhI CCWWGG 1 cut(s) 811
FaeI CATG 6 cut(s) 184, 486, 545, 593, 996, 1073
FalI AAGNNNNNCTT 2 cut(s) 852, 884
FaqI GGGAC 2 cut(s) 604, 683
FatI CATG 6 cut(s) 180, 482, 541, 589, 992, 1069
FauNDI CATATG 1 cut(s) 679
FbaI TGATCA 1 cut(s) 982
FblI GTMKAC 2 cut(s) 399, 702
Fnu4HI GCNGC 3 cut(s) 252, 327, 494
FokI GGATG 2 cut(s) 1049, 1196
FriOI GRGCYC 1 cut(s) 566
Fsp4HI GCNGC 3 cut(s) 252, 327, 494
FspBI CTAG 1 cut(s) 107
GlaI GCGC 2 cut(s) 837, 1238
GluI GCNGC 3 cut(s) 252, 327, 494
GsaI CCCAGC 1 cut(s) 563
HaeIII GGCC 7 cut(s) 276, 380, 418, 452, 564, 748, 789
HapII CCGG 4 cut(s) 449, 740, 776, 972
HgaI GACGC 1 cut(s) 1208
HhaI GCGC 2 cut(s) 838, 1239
Hin1II CATG 6 cut(s) 184, 486, 545, 593, 996, 1073
Hin6I GCGC 2 cut(s) 836, 1237
HinP1I GCGC 2 cut(s) 836, 1237
HindIII AAGCTT 2 cut(s) 372, 893
HinfI GANTC 8 cut(s) 61, 89, 164, 199, 302, 467, 719, 1148
HpaII CCGG 4 cut(s) 449, 740, 776, 972
HphI GGTGA 6 cut(s) 70, 401, 956, 1061, 1082, 1214
Hpy166II GTNNAC 4 cut(s) 400, 582, 703, 1042
Hpy188I TCNGA 4 cut(s) 204, 735, 1155, 1204
Hpy188III TCNNGA 5 cut(s) 95, 122, 668, 860, 972
Hpy8I GTNNAC 4 cut(s) 400, 582, 703, 1042
HpyAV CCTTC 4 cut(s) 4, 48, 216, 1198
HpyCH4III ACNGT 2 cut(s) 480, 855
HpyCH4IV ACGT 2 cut(s) 402, 609
HpyCH4V TGCA 8 cut(s) 34, 160, 254, 473, 582, 650, 770, 1131
HpyF10VI GCNNNNNNNGC 2 cut(s) 79, 499
HpyF3I CTNAG 4 cut(s) 50, 96, 891, 1209
HpySE526I ACGT 2 cut(s) 402, 609
Hsp92II CATG 6 cut(s) 184, 486, 545, 593, 996, 1073
HspAI GCGC 2 cut(s) 836, 1237
Kpn2I TCCGGA 1 cut(s) 971
Ksp22I TGATCA 1 cut(s) 982
Kzo9I GATC 5 cut(s) 360, 613, 664, 982, 1072
LmnI GCTCC 3 cut(s) 79, 334, 490
Lsp1109I GCAGC 3 cut(s) 238, 313, 480
LweI GCATC 2 cut(s) 637, 1174
MaeI CTAG 1 cut(s) 107
MaeII ACGT 2 cut(s) 402, 609
MaeIII GTNAC 5 cut(s) 240, 389, 602, 824, 1220
MalI GATC 5 cut(s) 362, 615, 666, 984, 1074
MboI GATC 5 cut(s) 360, 613, 664, 982, 1072
MboII GAAGA 2 cut(s) 217, 1067
MhlI GDGCHC 2 cut(s) 566, 584
MlsI TGGCCA 1 cut(s) 380
MluCI AATT 8 cut(s) 35, 173, 260, 635, 953, 978, 1108, 1174
MluNI TGGCCA 1 cut(s) 380
MlyI GAGTC 2 cut(s) 713, 1157
MmeI TCCRAC 2 cut(s) 166, 333
Mox20I TGGCCA 1 cut(s) 380
MroI TCCGGA 1 cut(s) 971
MroXI GAANNNNTTC 1 cut(s) 198
MscI TGGCCA 1 cut(s) 380
MseI TTAA 4 cut(s) 39, 66, 870, 1107
MslI CAYNNNNRTG 2 cut(s) 628, 1047
Msp20I TGGCCA 1 cut(s) 380
MspA1I CMGCKG 1 cut(s) 218
MspI CCGG 4 cut(s) 449, 740, 776, 972
MspR9I CCNGG 3 cut(s) 333, 777, 1181
MvaI CCWGG 2 cut(s) 333, 1181
MwoI GCNNNNNNNGC 2 cut(s) 79, 499
NciI CCSGG 1 cut(s) 777
NdeI CATATG 1 cut(s) 679
NdeII GATC 5 cut(s) 360, 613, 664, 982, 1072
NlaIII CATG 6 cut(s) 184, 486, 545, 593, 996, 1073
NlaIV GGNNCC 1 cut(s) 564
NmuCI GTSAC 2 cut(s) 389, 1220
NspV TTCGAA 1 cut(s) 167
OliI CACNNNNGTG 1 cut(s) 1047
PaeR7I CTCGAG 1 cut(s) 74
PceI AGGCCT 1 cut(s) 789
PdmI GAANNNNTTC 1 cut(s) 198
PfeI GAWTC 6 cut(s) 61, 89, 164, 199, 302, 467
PfoI TCCNGGA 1 cut(s) 1179
PkrI GCNGC 3 cut(s) 253, 328, 495
PleI GAGTC 2 cut(s) 713, 1156
PpsI GAGTC 2 cut(s) 713, 1156
Ppu21I YACGTR 1 cut(s) 403
PshBI ATTAAT 1 cut(s) 1107
Psp6I CCWGG 2 cut(s) 331, 1179
PspEI GGTNACC 1 cut(s) 240
PspFI CCCAGC 1 cut(s) 559
PspGI CCWGG 2 cut(s) 331, 1179
PspN4I GGNNCC 1 cut(s) 564
PspOMI GGGCCC 1 cut(s) 562
PspPI GGNCC 4 cut(s) 353, 562, 563, 747
PspXI VCTCGAGB 1 cut(s) 74
PvuII CAGCTG 1 cut(s) 218
RsaI GTAC 3 cut(s) 29, 179, 436
RsaNI GTAC 3 cut(s) 28, 178, 435
RseI CAYNNNNRTG 2 cut(s) 628, 1047
SaqAI TTAA 4 cut(s) 39, 66, 870, 1107
SatI GCNGC 3 cut(s) 252, 327, 494
Sau3AI GATC 5 cut(s) 360, 613, 664, 982, 1072
Sau96I GGNCC 4 cut(s) 353, 562, 563, 747
SchI GAGTC 2 cut(s) 713, 1157
ScrFI CCNGG 3 cut(s) 333, 777, 1181
SduI GDGCHC 2 cut(s) 566, 584
SfaNI GCATC 2 cut(s) 637, 1174
SfcI CTRYAG 1 cut(s) 1005
Sfr274I CTCGAG 1 cut(s) 74
SfuI TTCGAA 1 cut(s) 167
SinI GGWCC 1 cut(s) 353
SlaI CTCGAG 1 cut(s) 74
SmiMI CAYNNNNRTG 2 cut(s) 628, 1047
SmlI CTYRAG 1 cut(s) 74
SmoI CTYRAG 1 cut(s) 74
SnaBI TACGTA 1 cut(s) 403
SpeI ACTAGT 1 cut(s) 106
Sse9I AATT 8 cut(s) 35, 173, 260, 635, 953, 978, 1108, 1174
SseBI AGGCCT 1 cut(s) 789
SsiI CCGC 2 cut(s) 1034, 1243
SspI AATATT 1 cut(s) 1105
SspMI CTAG 1 cut(s) 107
StuI AGGCCT 1 cut(s) 789
StyD4I CCNGG 3 cut(s) 331, 775, 1179
StyI CCWWGG 1 cut(s) 811
TaaI ACNGT 2 cut(s) 480, 855
TaiI ACGT 2 cut(s) 405, 612
TaqI TCGA 4 cut(s) 15, 75, 167, 430
TasI AATT 8 cut(s) 35, 173, 260, 635, 953, 978, 1108, 1174
TatI WGTACW 2 cut(s) 27, 177
TfiI GAWTC 6 cut(s) 61, 89, 164, 199, 302, 467
Tru1I TTAA 4 cut(s) 39, 66, 870, 1107
Tru9I TTAA 4 cut(s) 39, 66, 870, 1107
TscAI CASTG 1 cut(s) 732
TseFI GTSAC 2 cut(s) 389, 1220
TseI GCWGC 3 cut(s) 251, 326, 493
Tsp45I GTSAC 2 cut(s) 389, 1220
TspDTI ATGAA 5 cut(s) 225, 539, 628, 696, 981
TspGWI ACGGA 1 cut(s) 327
TspRI CASTG 1 cut(s) 732
VneI GTGCAC 1 cut(s) 580
VpaK11BI GGWCC 1 cut(s) 353
VspI ATTAAT 1 cut(s) 1107
XapI RAATTY 2 cut(s) 635, 953
XhoI CTCGAG 1 cut(s) 74
XmiI GTMKAC 2 cut(s) 399, 702
XmnI GAANNNNTTC 1 cut(s) 198
XspI CTAG 1 cut(s) 107
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.