Rh2CG381800

IGR

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0001040
Physical Location & Seq
Forward (+)
52112717 .. 52116683
3967 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG381800.1

Sequence Viewer

Length: 1500 bp
ATGGGGGAGAAGGTCGAAAACTATTTGTACTTGCAATTTAAGGATCATCTTAGAAGGGTGAATCTTAACAAGCTCGAGGAGCAAAAGGAATCTCCTGAGTTTGAACTAGTGGCAGATTTTCTGGAGGAGAAACTCCCCTATGGAATGTGTGTTTTTTTGCAAGATTCGAATAAATTGTACATGGTTGGAGGAGAAAGGATTCGGAAGAATGAAAGAGCTGGAAGGTATTGGTGGCGAGGATTTCTCGATGCTGCAAACGAACTTGACTCCTCTGGCCTCAGTTGTGATGTTTTTGTGTTAGATTCCTCCAACGGAAAAATCTCTGCTGCTCCTGGTTTTCCAAAACCAAAAGGACCAAAGATCAATGCCAGAGCTTTGGCCACCATGGGTGACAAGGTATACGTAATGAGTTTTCGGCCATATTACTATCACGGTACGCTTCCATCTCCGGCCTTTGAGTGCTATGATTCTGCAAAACAGTCATGGGAGGAGCTGCCTGTGCCTCCAAATTACTATTGTTCGTCTTCTGCTGCTACTACTTATGGGTTTCATTTTGTGCTGGGCCCAAAGATTTATTTGTGCACTCCTCATGGCTTCTTTTGTTACAACGTGGATCAATCCCCACCTAAATGGGAATTCATAGAAGATGCAGAATGGCAGTTTGATCAGCTCCCCTCATATAATTCAGAAGAGGAAATGGAAGACAACAAGATTGAGGACTACTCACTGCCTCTGATACCGGCTTTGCCCTACCGTTCTATATGTTTTGCAGAACCCGGAAAAGGGGGCCTCGCCGTTGGCTATACTGCTCAAGGATTAGTGGGTTACTTCTTTGCGCCTAATGGCAACCTACTGTATCAAGAGCCACTCAATCAGTTGAACAGCAACTCTAAGCTTCCCTCCCCCAATAGCTATTTCTTTGGTGGAAAAGTTTGCTATGTAGGTGAAAACAAATTCTGTCTCATTCTATCTGGAGAAATTGATCAAACTTCATGGTCGTGGTCGTTAGCTGTAGCAACTTTTGATGTGAAATGTAAAGCTAATGTTCACAATGGTGATGGAGGAGGGAAACACTTCTACCATGATCTTCCTCTGTGTTATCGTCAGTTTGACAATATTAATTCGTGTTTTTCTAATGCTTGCAAATATGAGAACAGCGAGTCTCCGAGGCTTTATGATTACCTTAATTTCCAGGATGCCTTCGTCATATCCTCCGAGCCTAAGAGCCAGCGTCACCACAACCACAAGCGGAAGCGGATTATGATGGGTGTAGAGAAGGTTGGGATACCAGAATTATTAAATGGAGTAGGCAATGGGGTGGAAGCTCATGTGGCTAAAATTGAATCTGAGATTGGTGACTTTCAGAAGCTGCTGATCACTTGTAGTCTCATACTGAAGAAACTTGTCATCCCTATCAAACAAGGGAAGCTGATCTTGAAATACACCCACAAGTATAGGCTGGGACTCTGGAGGCCTCTAGCTCAGCCAATGAAATCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
IGR

Protein Analysis

499

Amino Acids

56.74

Weight (kDa)

6.48

Isoelectric Point (pI)

51.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1668 PF07893 98 - 214 7.1e-06 Protein of unknown function (DUF1668)
SAM_Ribosomal_mS41 PF09597 429 - 488 9.8e-10 Small ribosomal subunit protein mS41 SAM domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 399
AciI CCGC 2 cut(s) 1249, 1255
AclWI GGATC 2 cut(s) 51, 621
AcoI YGGCCR 2 cut(s) 378, 416
AcsI RAATTY 2 cut(s) 635, 953
AcuI CTGAAG 1 cut(s) 1415
AfaI GTAC 3 cut(s) 29, 179, 436
AfiI CCNNNNNNNGG 2 cut(s) 782, 783
AgsI TTSAA 4 cut(s) 104, 880, 1343, 1438
AhlI ACTAGT 1 cut(s) 106
AjnI CCWGG 2 cut(s) 331, 1191
AjuI GAANNNNNNNTTGG 4 cut(s) 899, 931, 1335, 1367
AleI CACNNNNGTG 1 cut(s) 1053
Alw21I GWGCWC 1 cut(s) 584
Alw26I GTCTC 3 cut(s) 965, 1167, 1391
Alw44I GTGCAC 1 cut(s) 580
AlwI GGATC 2 cut(s) 51, 621
AlwNI CAGNNNCTG 1 cut(s) 1369
Ama87I CYCGRG 1 cut(s) 74
AoxI GGCC 7 cut(s) 274, 378, 416, 450, 562, 787, 1472
ApaI GGGCCC 1 cut(s) 566
ApaLI GTGCAC 1 cut(s) 580
ApeKI GCWGC 5 cut(s) 251, 326, 493, 530, 1369
ApoI RAATTY 2 cut(s) 635, 953
AseI ATTAAT 1 cut(s) 1119
Asp700I GAANNNNTTC 2 cut(s) 198, 1073
AspLEI GCGC 1 cut(s) 838
AspS9I GGNCC 4 cut(s) 353, 562, 563, 787
AsuC2I CCSGG 1 cut(s) 777
AsuHPI GGTGA 6 cut(s) 70, 401, 956, 1067, 1226, 1367
AsuII TTCGAA 1 cut(s) 167
AvaI CYCGRG 1 cut(s) 74
AvaII GGWCC 1 cut(s) 353
BaeGI GKGCMC 2 cut(s) 566, 584
BalI TGGCCA 1 cut(s) 380
BanII GRGCYC 1 cut(s) 566
BbsI GAAGAC 2 cut(s) 516, 708
Bbv12I GWGCWC 1 cut(s) 584
BbvI GCAGC 5 cut(s) 238, 313, 480, 517, 1356
BccI CCATC 3 cut(s) 451, 1052, 1258
BceAI ACGGC 1 cut(s) 779
BciT130I CCWGG 2 cut(s) 333, 1193
BciVI GTATCC 1 cut(s) 1278
BclI TGATCA 3 cut(s) 664, 982, 1374
BcnI CCSGG 1 cut(s) 777
BcoDI GTCTC 3 cut(s) 965, 1167, 1391
BcuI ACTAGT 1 cut(s) 106
BfaI CTAG 3 cut(s) 107, 1478, 1498
BfmI CTRYAG 1 cut(s) 1011
BfuI GTATCC 1 cut(s) 1278
BisI GCNGC 5 cut(s) 252, 327, 494, 531, 1370
BlpI GCTNAGC 1 cut(s) 1482
BlsI GCNGC 5 cut(s) 253, 328, 495, 532, 1371
Bme1390I CCNGG 3 cut(s) 333, 777, 1193
Bme18I GGWCC 1 cut(s) 353
BmeT110I CYCGRG 1 cut(s) 74
BmgT120I GGNCC 4 cut(s) 353, 562, 563, 787
BmiI GGNNCC 2 cut(s) 564, 788
BmrFI CCNGG 3 cut(s) 333, 777, 1193
BmsI GCATC 3 cut(s) 238, 637, 1186
BpiI GAAGAC 2 cut(s) 516, 708
BplI GAGNNNNNCTC 4 cut(s) 228, 260, 707, 739
BpmI CTGGAG 3 cut(s) 143, 993, 1489
Bpu1102I GCTNAGC 1 cut(s) 1482
Bpu14I TTCGAA 1 cut(s) 167
BpuEI CTTGAG 1 cut(s) 795
BpuMI CCSGG 1 cut(s) 777
BsaAI YACGTR 1 cut(s) 403
BsaJI CCNNGG 2 cut(s) 384, 1166
Bsc4I CCNNNNNNNGG 2 cut(s) 782, 783
Bse118I RCCGGY 1 cut(s) 739
Bse3DI GCAATG 1 cut(s) 1318
BseBI CCWGG 2 cut(s) 333, 1193
BseDI CCNNGG 2 cut(s) 384, 1166
BseGI GGATG 2 cut(s) 1201, 1407
BseLI CCNNNNNNNGG 2 cut(s) 782, 783
BseMI GCAATG 1 cut(s) 1318
BseMII CTCAG 4 cut(s) 87, 292, 1338, 1496
BseRI GAGGAG 7 cut(s) 92, 140, 204, 259, 503, 576, 1077
BseSI GKGCMC 2 cut(s) 566, 584
BseXI GCAGC 5 cut(s) 238, 313, 480, 517, 1356
BseYI CCCAGC 2 cut(s) 559, 1459
BshFI GGCC 7 cut(s) 276, 380, 418, 452, 564, 789, 1474
BsiHKAI GWGCWC 1 cut(s) 584
BsiHKCI CYCGRG 1 cut(s) 74
BsiSI CCGG 3 cut(s) 449, 740, 777
BslFI GGGAC 1 cut(s) 1476
BslI CCNNNNNNNGG 2 cut(s) 782, 783
BsmAI GTCTC 3 cut(s) 965, 1167, 1391
BsmFI GGGAC 1 cut(s) 1476
BsnI GGCC 7 cut(s) 276, 380, 418, 452, 564, 789, 1474
BsoBI CYCGRG 1 cut(s) 74
Bsp119I TTCGAA 1 cut(s) 167
Bsp120I GGGCCC 1 cut(s) 562
Bsp1286I GDGCHC 2 cut(s) 566, 584
Bsp1407I TGTACA 1 cut(s) 177
Bsp143I GATC 8 cut(s) 43, 360, 613, 664, 982, 1084, 1374, 1431
Bsp1720I GCTNAGC 1 cut(s) 1482
Bsp19I CCATGG 1 cut(s) 384
BspACI CCGC 2 cut(s) 1249, 1255
BspANI GGCC 7 cut(s) 276, 380, 418, 452, 564, 789, 1474
BspCNI CTCAG 4 cut(s) 88, 291, 1339, 1495
BspLI GGNNCC 2 cut(s) 564, 788
BspPI GGATC 2 cut(s) 51, 621
BspT104I TTCGAA 1 cut(s) 167
BsrDI GCAATG 1 cut(s) 1318
BsrFI RCCGGY 1 cut(s) 739
BsrGI TGTACA 1 cut(s) 177
BssAI RCCGGY 1 cut(s) 739
BssECI CCNNGG 2 cut(s) 384, 1166
BssMI GATC 8 cut(s) 43, 360, 613, 664, 982, 1084, 1374, 1431
BssNAI GTATAC 1 cut(s) 400
BssT1I CCWWGG 1 cut(s) 384
Bst1107I GTATAC 1 cut(s) 400
Bst2UI CCWGG 2 cut(s) 333, 1193
Bst4CI ACNGT 4 cut(s) 434, 480, 755, 855
Bst6I CTCTTC 1 cut(s) 684
BstAUI TGTACA 1 cut(s) 177
BstBAI YACGTR 1 cut(s) 403
BstBI TTCGAA 1 cut(s) 167
BstC8I GCNNGC 2 cut(s) 1141, 1229
BstDEI CTNAG 7 cut(s) 50, 96, 278, 891, 1221, 1347, 1482
BstDSI CCRYGG 1 cut(s) 384
BstF5I GGATG 2 cut(s) 1201, 1407
BstHHI GCGC 1 cut(s) 838
BstKTI GATC 8 cut(s) 46, 363, 616, 667, 985, 1087, 1377, 1434
BstMAI GTCTC 3 cut(s) 965, 1167, 1391
BstMBI GATC 8 cut(s) 43, 360, 613, 664, 982, 1084, 1374, 1431
BstMWI GCNNNNNNNGC 3 cut(s) 79, 499, 1331
BstNI CCWGG 2 cut(s) 333, 1193
BstSCI CCNGG 3 cut(s) 331, 775, 1191
BstSFI CTRYAG 1 cut(s) 1011
BstSLI GKGCMC 2 cut(s) 566, 584
BstSNI TACGTA 1 cut(s) 403
BstV1I GCAGC 5 cut(s) 238, 313, 480, 517, 1356
BstV2I GAAGAC 2 cut(s) 516, 708
BstXI CCANNNNNNTGG 2 cut(s) 376, 630
BstZ17I GTATAC 1 cut(s) 400
BsuI GTATCC 1 cut(s) 1278
BsuRI GGCC 7 cut(s) 276, 380, 418, 452, 564, 789, 1474
BtgI CCRYGG 1 cut(s) 384
BtsCI GGATG 2 cut(s) 1201, 1407
BtsI GCAGTG 1 cut(s) 725
BtsIMutI CAGTG 1 cut(s) 725
Cac8I GCNNGC 2 cut(s) 1141, 1229
CaiI CAGNNNCTG 1 cut(s) 1369
CfoI GCGC 1 cut(s) 838
Cfr10I RCCGGY 1 cut(s) 739
Cfr13I GGNCC 4 cut(s) 353, 562, 563, 787
CseI GACGC 1 cut(s) 1220
Csp6I GTAC 3 cut(s) 28, 178, 435
CviAII CATG 7 cut(s) 181, 385, 483, 590, 993, 1082, 1328
CviQI GTAC 3 cut(s) 28, 178, 435
DdeI CTNAG 7 cut(s) 50, 96, 278, 891, 1221, 1347, 1482
DpnI GATC 8 cut(s) 45, 362, 615, 666, 984, 1086, 1376, 1433
DpnII GATC 8 cut(s) 43, 360, 613, 664, 982, 1084, 1374, 1431
EaeI YGGCCR 2 cut(s) 378, 416
Eam1104I CTCTTC 1 cut(s) 684
EarI CTCTTC 1 cut(s) 684
Eco105I TACGTA 1 cut(s) 403
Eco130I CCWWGG 1 cut(s) 384
Eco147I AGGCCT 1 cut(s) 1474
Eco24I GRGCYC 1 cut(s) 566
Eco47I GGWCC 1 cut(s) 353
Eco57I CTGAAG 1 cut(s) 1415
Eco88I CYCGRG 1 cut(s) 74
EcoO109I RGGNCCY 1 cut(s) 787
EcoRI GAATTC 1 cut(s) 635
EcoRII CCWGG 2 cut(s) 331, 1191
EcoT14I CCWWGG 1 cut(s) 384
EcoT38I GRGCYC 1 cut(s) 566
ErhI CCWWGG 1 cut(s) 384
FaeI CATG 7 cut(s) 184, 388, 486, 593, 996, 1085, 1331
FalI AAGNNNNNCTT 2 cut(s) 1418, 1450
FaqI GGGAC 1 cut(s) 1476
FatI CATG 7 cut(s) 180, 384, 482, 589, 992, 1081, 1327
FbaI TGATCA 3 cut(s) 664, 982, 1374
FblI GTMKAC 1 cut(s) 399
Fnu4HI GCNGC 5 cut(s) 252, 327, 494, 531, 1370
FokI GGATG 2 cut(s) 1208, 1394
FriOI GRGCYC 1 cut(s) 566
Fsp4HI GCNGC 5 cut(s) 252, 327, 494, 531, 1370
FspBI CTAG 3 cut(s) 107, 1478, 1498
GlaI GCGC 1 cut(s) 837
GluI GCNGC 5 cut(s) 252, 327, 494, 531, 1370
GsaI CCCAGC 2 cut(s) 563, 1463
GsuI CTGGAG 3 cut(s) 143, 993, 1489
HaeIII GGCC 7 cut(s) 276, 380, 418, 452, 564, 789, 1474
HapII CCGG 3 cut(s) 449, 740, 777
HgaI GACGC 1 cut(s) 1220
HhaI GCGC 1 cut(s) 838
Hin1II CATG 7 cut(s) 184, 388, 486, 593, 996, 1085, 1331
Hin6I GCGC 1 cut(s) 836
HinP1I GCGC 1 cut(s) 836
HindIII AAGCTT 1 cut(s) 893
HpaII CCGG 3 cut(s) 449, 740, 777
HphI GGTGA 6 cut(s) 70, 401, 956, 1067, 1226, 1367
Hpy166II GTNNAC 3 cut(s) 400, 582, 1048
Hpy188I TCNGA 7 cut(s) 204, 688, 735, 1167, 1216, 1348, 1365
Hpy188III TCNNGA 7 cut(s) 95, 122, 245, 860, 972, 1435, 1468
Hpy8I GTNNAC 3 cut(s) 400, 582, 1048
HpyAV CCTTC 5 cut(s) 4, 48, 216, 1210, 1270
HpyCH4III ACNGT 4 cut(s) 434, 480, 755, 855
HpyCH4IV ACGT 2 cut(s) 402, 609
HpyCH4V TGCA 8 cut(s) 34, 160, 254, 473, 582, 650, 770, 1143
HpyF10VI GCNNNNNNNGC 3 cut(s) 79, 499, 1331
HpyF3I CTNAG 7 cut(s) 50, 96, 278, 891, 1221, 1347, 1482
HpySE526I ACGT 2 cut(s) 402, 609
Hsp92II CATG 7 cut(s) 184, 388, 486, 593, 996, 1085, 1331
HspAI GCGC 1 cut(s) 836
Ksp22I TGATCA 3 cut(s) 664, 982, 1374
Kzo9I GATC 8 cut(s) 43, 360, 613, 664, 982, 1084, 1374, 1431
LmnI GCTCC 4 cut(s) 79, 334, 490, 675
Lsp1109I GCAGC 5 cut(s) 238, 313, 480, 517, 1356
LweI GCATC 3 cut(s) 238, 637, 1186
MaeI CTAG 3 cut(s) 107, 1478, 1498
MaeII ACGT 2 cut(s) 402, 609
MaeIII GTNAC 5 cut(s) 389, 602, 824, 1232, 1355
MalI GATC 8 cut(s) 45, 362, 615, 666, 984, 1086, 1376, 1433
MboI GATC 8 cut(s) 43, 360, 613, 664, 982, 1084, 1374, 1431
MboII GAAGA 7 cut(s) 217, 516, 656, 701, 713, 1079, 1408
MhlI GDGCHC 2 cut(s) 566, 584
MlsI TGGCCA 1 cut(s) 380
MluNI TGGCCA 1 cut(s) 380
MlyI GAGTC 3 cut(s) 260, 1169, 1458
MmeI TCCRAC 2 cut(s) 166, 333
Mox20I TGGCCA 1 cut(s) 380
MroXI GAANNNNTTC 2 cut(s) 198, 1073
MscI TGGCCA 1 cut(s) 380
MseI TTAA 5 cut(s) 39, 66, 1119, 1185, 1298
MslI CAYNNNNRTG 3 cut(s) 628, 997, 1053
Msp20I TGGCCA 1 cut(s) 380
MspI CCGG 3 cut(s) 449, 740, 777
MspR9I CCNGG 3 cut(s) 333, 777, 1193
MvaI CCWGG 2 cut(s) 333, 1193
MwoI GCNNNNNNNGC 3 cut(s) 79, 499, 1331
NciI CCSGG 1 cut(s) 777
NcoI CCATGG 1 cut(s) 384
NdeII GATC 8 cut(s) 43, 360, 613, 664, 982, 1084, 1374, 1431
NlaIII CATG 7 cut(s) 184, 388, 486, 593, 996, 1085, 1331
NlaIV GGNNCC 2 cut(s) 564, 788
NmuCI GTSAC 3 cut(s) 389, 1232, 1355
NspV TTCGAA 1 cut(s) 167
OliI CACNNNNGTG 1 cut(s) 1053
PaeR7I CTCGAG 1 cut(s) 74
PceI AGGCCT 1 cut(s) 1474
PdmI GAANNNNTTC 2 cut(s) 198, 1073
PfeI GAWTC 7 cut(s) 61, 89, 164, 199, 302, 467, 1343
PfoI TCCNGGA 1 cut(s) 1191
PkrI GCNGC 5 cut(s) 253, 328, 495, 532, 1371
PleI GAGTC 3 cut(s) 260, 1168, 1458
PpsI GAGTC 3 cut(s) 260, 1168, 1458
Ppu21I YACGTR 1 cut(s) 403
PshBI ATTAAT 1 cut(s) 1119
Psp6I CCWGG 2 cut(s) 331, 1191
PspFI CCCAGC 2 cut(s) 559, 1459
PspGI CCWGG 2 cut(s) 331, 1191
PspN4I GGNNCC 2 cut(s) 564, 788
PspOMI GGGCCC 1 cut(s) 562
PspPI GGNCC 4 cut(s) 353, 562, 563, 787
PspXI VCTCGAGB 1 cut(s) 74
PstNI CAGNNNCTG 1 cut(s) 1369
RsaI GTAC 3 cut(s) 29, 179, 436
RsaNI GTAC 3 cut(s) 28, 178, 435
RseI CAYNNNNRTG 3 cut(s) 628, 997, 1053
SaqAI TTAA 5 cut(s) 39, 66, 1119, 1185, 1298
SatI GCNGC 5 cut(s) 252, 327, 494, 531, 1370
Sau3AI GATC 8 cut(s) 43, 360, 613, 664, 982, 1084, 1374, 1431
Sau96I GGNCC 4 cut(s) 353, 562, 563, 787
SchI GAGTC 3 cut(s) 260, 1169, 1458
ScrFI CCNGG 3 cut(s) 333, 777, 1193
SduI GDGCHC 2 cut(s) 566, 584
SfaNI GCATC 3 cut(s) 238, 637, 1186
SfcI CTRYAG 1 cut(s) 1011
Sfr274I CTCGAG 1 cut(s) 74
SfuI TTCGAA 1 cut(s) 167
SinI GGWCC 1 cut(s) 353
SlaI CTCGAG 1 cut(s) 74
SmiMI CAYNNNNRTG 3 cut(s) 628, 997, 1053
SmlI CTYRAG 2 cut(s) 74, 810
SmoI CTYRAG 2 cut(s) 74, 810
SnaBI TACGTA 1 cut(s) 403
SpeI ACTAGT 1 cut(s) 106
SseBI AGGCCT 1 cut(s) 1474
SsiI CCGC 2 cut(s) 1249, 1255
SspI AATATT 1 cut(s) 1117
SspMI CTAG 3 cut(s) 107, 1478, 1498
StuI AGGCCT 1 cut(s) 1474
StyD4I CCNGG 3 cut(s) 331, 775, 1191
StyI CCWWGG 1 cut(s) 384
TaaI ACNGT 4 cut(s) 434, 480, 755, 855
TaiI ACGT 2 cut(s) 405, 612
TaqI TCGA 4 cut(s) 15, 75, 167, 246
TatI WGTACW 2 cut(s) 27, 177
TfiI GAWTC 7 cut(s) 61, 89, 164, 199, 302, 467, 1343
Tru1I TTAA 5 cut(s) 39, 66, 1119, 1185, 1298
Tru9I TTAA 5 cut(s) 39, 66, 1119, 1185, 1298
TscAI CASTG 1 cut(s) 732
TseFI GTSAC 3 cut(s) 389, 1232, 1355
TseI GCWGC 5 cut(s) 251, 326, 493, 530, 1369
Tsp45I GTSAC 3 cut(s) 389, 1232, 1355
TspDTI ATGAA 4 cut(s) 225, 539, 628, 981
TspGWI ACGGA 1 cut(s) 327
TspRI CASTG 1 cut(s) 732
VneI GTGCAC 1 cut(s) 580
VpaK11BI GGWCC 1 cut(s) 353
VspI ATTAAT 1 cut(s) 1119
XapI RAATTY 2 cut(s) 635, 953
XhoI CTCGAG 1 cut(s) 74
XmiI GTMKAC 1 cut(s) 399
XmnI GAANNNNTTC 2 cut(s) 198, 1073
XspI CTAG 3 cut(s) 107, 1478, 1498
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.