Rh2BG412400

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0001040
Physical Location & Seq
Reverse (-)
58315231 .. 58315716
486 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG412400.1

Sequence Viewer

Length: 486 bp
ATGGGGGAGAAGGTCGAAAACTATTTGTACTTGCAATTTAAGGAACATCTTAGAAGGGTGAATCTTAACAAGCTCGAGGAGCAAAAGGAATCTCCTGAGTTTGAACTAGTGGCAGATTTTCTGGAGGAGAAACTCCCCTATGGAATGTGTGTTTTTTTGCAAGATTCGAATAAATTGTACATGGTTGGAGGAGAAAGGATTCGGAAGAATGAAACAGCTGGAAGGTATTGGTGGCAAGGATTACCCGTTGCTGCAAACGAATTTGACCCCTCTGGCCTCACTGGTGATCTTTTTGTGTTAGATTCCTCCAACGGAAAAATCTCTGCTGCTCCTGGTTTTCCAAAACCAAAAGGACCAAAGATCAATGCCAAAGCTTTGGCCACCATCGGTGACAAGGTATACGTAATGAGTTTCCGGCCATATTACTATCGAGGTACGCTTCCATCTCCGGCCTTTGAGTGCTATGATTCTGCAAAACAGTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

161

Amino Acids

18.29

Weight (kDa)

6.61

Isoelectric Point (pI)

53.32

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 399
AcoI YGGCCR 2 cut(s) 378, 416
AcsI RAATTY 1 cut(s) 260
AfaI GTAC 3 cut(s) 29, 179, 436
AgsI TTSAA 1 cut(s) 104
AhlI ACTAGT 1 cut(s) 106
AjnI CCWGG 1 cut(s) 331
AluBI AGCT 3 cut(s) 73, 218, 374
AluI AGCT 3 cut(s) 73, 218, 374
Ama87I CYCGRG 1 cut(s) 74
AoxI GGCC 4 cut(s) 274, 378, 416, 450
ApeKI GCWGC 2 cut(s) 251, 326
ApoI RAATTY 1 cut(s) 260
Asp700I GAANNNNTTC 1 cut(s) 198
AspS9I GGNCC 1 cut(s) 353
AsuHPI GGTGA 3 cut(s) 70, 296, 401
AsuII TTCGAA 1 cut(s) 167
AvaI CYCGRG 1 cut(s) 74
AvaII GGWCC 1 cut(s) 353
BalI TGGCCA 1 cut(s) 380
BbvI GCAGC 2 cut(s) 238, 313
BccI CCATC 2 cut(s) 392, 451
BciT130I CCWGG 1 cut(s) 333
BcuI ACTAGT 1 cut(s) 106
BfaI CTAG 1 cut(s) 107
BisI GCNGC 2 cut(s) 252, 327
BlsI GCNGC 2 cut(s) 253, 328
Bme1390I CCNGG 1 cut(s) 333
Bme18I GGWCC 1 cut(s) 353
BmeT110I CYCGRG 1 cut(s) 74
BmgT120I GGNCC 1 cut(s) 353
BmrFI CCNGG 1 cut(s) 333
BpmI CTGGAG 1 cut(s) 143
Bpu14I TTCGAA 1 cut(s) 167
BsaAI YACGTR 1 cut(s) 403
Bse1I ACTGG 1 cut(s) 286
BseBI CCWGG 1 cut(s) 333
BseMII CTCAG 1 cut(s) 87
BseNI ACTGG 1 cut(s) 286
BseRI GAGGAG 3 cut(s) 92, 140, 204
BseXI GCAGC 2 cut(s) 238, 313
BshFI GGCC 4 cut(s) 276, 380, 418, 452
BsiHKCI CYCGRG 1 cut(s) 74
BsiSI CCGG 2 cut(s) 415, 449
BsnI GGCC 4 cut(s) 276, 380, 418, 452
BsoBI CYCGRG 1 cut(s) 74
Bsp119I TTCGAA 1 cut(s) 167
Bsp1407I TGTACA 1 cut(s) 177
Bsp143I GATC 2 cut(s) 286, 360
BspANI GGCC 4 cut(s) 276, 380, 418, 452
BspCNI CTCAG 1 cut(s) 88
BspHI TCATGA 1 cut(s) 482
BspT104I TTCGAA 1 cut(s) 167
BsrGI TGTACA 1 cut(s) 177
BsrI ACTGG 1 cut(s) 286
BssMI GATC 2 cut(s) 286, 360
BssNAI GTATAC 1 cut(s) 400
Bst1107I GTATAC 1 cut(s) 400
Bst2UI CCWGG 1 cut(s) 333
Bst4CI ACNGT 1 cut(s) 480
BstAUI TGTACA 1 cut(s) 177
BstBAI YACGTR 1 cut(s) 403
BstBI TTCGAA 1 cut(s) 167
BstDEI CTNAG 2 cut(s) 50, 96
BstKTI GATC 2 cut(s) 289, 363
BstMBI GATC 2 cut(s) 286, 360
BstMWI GCNNNNNNNGC 1 cut(s) 79
BstNI CCWGG 1 cut(s) 333
BstSCI CCNGG 1 cut(s) 331
BstSNI TACGTA 1 cut(s) 403
BstV1I GCAGC 2 cut(s) 238, 313
BstXI CCANNNNNNTGG 1 cut(s) 376
BstZ17I GTATAC 1 cut(s) 400
BsuRI GGCC 4 cut(s) 276, 380, 418, 452
BtsIMutI CAGTG 1 cut(s) 279
CciI TCATGA 1 cut(s) 482
Cfr13I GGNCC 1 cut(s) 353
Csp6I GTAC 3 cut(s) 28, 178, 435
CviAII CATG 2 cut(s) 181, 483
CviJI RGCY 7 cut(s) 73, 218, 276, 374, 380, 418, 452
CviKI_1 RGCY 7 cut(s) 73, 218, 276, 374, 380, 418, 452
CviQI GTAC 3 cut(s) 28, 178, 435
DdeI CTNAG 2 cut(s) 50, 96
DpnI GATC 2 cut(s) 288, 362
DpnII GATC 2 cut(s) 286, 360
EaeI YGGCCR 2 cut(s) 378, 416
Eco105I TACGTA 1 cut(s) 403
Eco47I GGWCC 1 cut(s) 353
Eco88I CYCGRG 1 cut(s) 74
EcoRII CCWGG 1 cut(s) 331
FaeI CATG 2 cut(s) 184, 486
FaiI YATR 6 cut(s) 141, 182, 400, 421, 465, 484
FatI CATG 2 cut(s) 180, 482
FblI GTMKAC 1 cut(s) 399
Fnu4HI GCNGC 2 cut(s) 252, 327
Fsp4HI GCNGC 2 cut(s) 252, 327
FspBI CTAG 1 cut(s) 107
GluI GCNGC 2 cut(s) 252, 327
GsuI CTGGAG 1 cut(s) 143
HaeIII GGCC 4 cut(s) 276, 380, 418, 452
HapII CCGG 2 cut(s) 415, 449
Hin1II CATG 2 cut(s) 184, 486
HindIII AAGCTT 1 cut(s) 372
HinfI GANTC 6 cut(s) 61, 89, 164, 199, 302, 467
HpaII CCGG 2 cut(s) 415, 449
HphI GGTGA 3 cut(s) 70, 296, 401
Hpy166II GTNNAC 1 cut(s) 400
Hpy188I TCNGA 1 cut(s) 204
Hpy188III TCNNGA 3 cut(s) 95, 122, 483
Hpy8I GTNNAC 1 cut(s) 400
HpyAV CCTTC 3 cut(s) 4, 48, 216
HpyCH4III ACNGT 1 cut(s) 480
HpyCH4IV ACGT 1 cut(s) 402
HpyCH4V TGCA 4 cut(s) 34, 160, 254, 473
HpyF10VI GCNNNNNNNGC 1 cut(s) 79
HpyF3I CTNAG 2 cut(s) 50, 96
HpySE526I ACGT 1 cut(s) 402
Hsp92II CATG 2 cut(s) 184, 486
Kzo9I GATC 2 cut(s) 286, 360
LmnI GCTCC 2 cut(s) 79, 334
LpnPI CCDG 9 cut(s) 107, 108, 204, 258, 267, 318, 345, 428, 462
Lsp1109I GCAGC 2 cut(s) 238, 313
MaeI CTAG 1 cut(s) 107
MaeII ACGT 1 cut(s) 402
MaeIII GTNAC 1 cut(s) 389
MalI GATC 2 cut(s) 288, 362
MboI GATC 2 cut(s) 286, 360
MboII GAAGA 1 cut(s) 217
MlsI TGGCCA 1 cut(s) 380
MluCI AATT 3 cut(s) 35, 173, 260
MluNI TGGCCA 1 cut(s) 380
MmeI TCCRAC 2 cut(s) 166, 333
MnlI CCTC 7 cut(s) 70, 118, 182, 280, 287, 316, 425
Mox20I TGGCCA 1 cut(s) 380
MroXI GAANNNNTTC 1 cut(s) 198
MscI TGGCCA 1 cut(s) 380
MseI TTAA 2 cut(s) 39, 66
Msp20I TGGCCA 1 cut(s) 380
MspA1I CMGCKG 1 cut(s) 218
MspI CCGG 2 cut(s) 415, 449
MspR9I CCNGG 1 cut(s) 333
MvaI CCWGG 1 cut(s) 333
MwoI GCNNNNNNNGC 1 cut(s) 79
NdeII GATC 2 cut(s) 286, 360
NlaIII CATG 2 cut(s) 184, 486
NmuCI GTSAC 1 cut(s) 389
NspV TTCGAA 1 cut(s) 167
PaeR7I CTCGAG 1 cut(s) 74
PagI TCATGA 1 cut(s) 482
PdmI GAANNNNTTC 1 cut(s) 198
PfeI GAWTC 6 cut(s) 61, 89, 164, 199, 302, 467
PkrI GCNGC 2 cut(s) 253, 328
Ppu21I YACGTR 1 cut(s) 403
Psp6I CCWGG 1 cut(s) 331
PspGI CCWGG 1 cut(s) 331
PspPI GGNCC 1 cut(s) 353
PspXI VCTCGAGB 1 cut(s) 74
PvuII CAGCTG 1 cut(s) 218
RsaI GTAC 3 cut(s) 29, 179, 436
RsaNI GTAC 3 cut(s) 28, 178, 435
SaqAI TTAA 2 cut(s) 39, 66
SatI GCNGC 2 cut(s) 252, 327
Sau3AI GATC 2 cut(s) 286, 360
Sau96I GGNCC 1 cut(s) 353
ScrFI CCNGG 1 cut(s) 333
SetI ASST 8 cut(s) 15, 75, 220, 227, 376, 399, 405, 436
Sfr274I CTCGAG 1 cut(s) 74
SfuI TTCGAA 1 cut(s) 167
SinI GGWCC 1 cut(s) 353
SlaI CTCGAG 1 cut(s) 74
SmlI CTYRAG 1 cut(s) 74
SmoI CTYRAG 1 cut(s) 74
SnaBI TACGTA 1 cut(s) 403
SpeI ACTAGT 1 cut(s) 106
Sse9I AATT 3 cut(s) 35, 173, 260
SspMI CTAG 1 cut(s) 107
StyD4I CCNGG 1 cut(s) 331
TaaI ACNGT 1 cut(s) 480
TaiI ACGT 1 cut(s) 405
TaqI TCGA 4 cut(s) 15, 75, 167, 430
TasI AATT 3 cut(s) 35, 173, 260
TatI WGTACW 2 cut(s) 27, 177
TfiI GAWTC 6 cut(s) 61, 89, 164, 199, 302, 467
Tru1I TTAA 2 cut(s) 39, 66
Tru9I TTAA 2 cut(s) 39, 66
TscAI CASTG 1 cut(s) 286
TseFI GTSAC 1 cut(s) 389
TseI GCWGC 2 cut(s) 251, 326
Tsp45I GTSAC 1 cut(s) 389
TspDTI ATGAA 1 cut(s) 225
TspGWI ACGGA 1 cut(s) 327
TspRI CASTG 1 cut(s) 286
VpaK11BI GGWCC 1 cut(s) 353
XapI RAATTY 1 cut(s) 260
XhoI CTCGAG 1 cut(s) 74
XmiI GTMKAC 1 cut(s) 399
XmnI GAANNNNTTC 1 cut(s) 198
XspI CTAG 1 cut(s) 107
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.