Rmu_sc0002070.1_g000013

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002070.1
OG0001040
Physical Location & Seq
Forward (+)
65217 .. 66365
1149 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0002070.1_g000013.1.cds

Sequence Viewer

Length: 1149 bp
atggttggaggagaaaggattcggaagaatgaaagagctggaaggtattggtggcgaggatttctcgatgctgcaaacgaacttgactctggcctcagttgtgatgtttttgtgttagattcctccaacggaaaaatctctgctgctcctggttttccaaaaccaaaaggaccaaagatcaatgccagagctttggccaccatgggtgacaaggtatacgtaatgagttttcggccatattactatcacggtacgcttccatctccggcctttgagtgctatgattctgcaaaacagtcatgggaggagctgcctgtgcctccaaattactattgttcgtcttctgctgctactacttatgggtttcattttgtgctgggcccaaagatttatttgtgcactcctcatggcttcttttgttacaacgtggatcaatccccacctaaatgggaattcatagaagatgcagaatggcagtttgatcagctcccctcatataattcagaagaggaaatggaagacaacaagattgaggactactcactgcctctgataccggctttgccctaccgttctatatgttttgcagaacccggaaaagggggcctcgccgttggctatactgctcaaggattagtgggttacttctttgcgcctaatggcaacctactgtatcaagagccactcaatcagttgaacagcaactctaagcttccctcccccaatagctatttctttggtggaaaagtttgctatgtaggtgaaaacaaattctgtctcattctatctggagaaattgatcaaacttcatggtcgtggtcgttagctgtagcaacttttgatgtgaaatgtaaagctaattttcacaatggtgatggaggagggaaacacttctaccatgatcttcctctgtgttatcatcagtttgacaatattaattcgtgtttttctaatgcttgcaaatatgagaacagcgagtctccgaggctttatgattaccttaatttccaggatgccttcgtcatgtacggttactctctcaccccaccccctccccctcttttagattttagtatattagcaactgttctcattgtgttctccgtgtgtctagtagtttcatggtttgatatgcatgatgtcccataa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

382

Amino Acids

42.99

Weight (kDa)

4.98

Isoelectric Point (pI)

52.31

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 216
AclWI GGATC 1 cut(s) 438
AcoI YGGCCR 2 cut(s) 195, 233
AcsI RAATTY 2 cut(s) 452, 770
AfaI GTAC 2 cut(s) 253, 1028
AfiI CCNNNNNNNGG 2 cut(s) 599, 600
AgsI TTSAA 1 cut(s) 697
AjnI CCWGG 2 cut(s) 148, 1008
AjuI GAANNNNNNNTTGG 2 cut(s) 716, 748
AleI CACNNNNGTG 1 cut(s) 870
AluBI AGCT 8 cut(s) 38, 191, 310, 487, 712, 729, 827, 857
AluI AGCT 8 cut(s) 38, 191, 310, 487, 712, 729, 827, 857
Alw21I GWGCWC 1 cut(s) 401
Alw26I GTCTC 2 cut(s) 782, 984
Alw44I GTGCAC 1 cut(s) 397
AlwI GGATC 1 cut(s) 438
AoxI GGCC 6 cut(s) 91, 195, 233, 267, 379, 604
ApaI GGGCCC 1 cut(s) 383
ApaLI GTGCAC 1 cut(s) 397
ApeKI GCWGC 4 cut(s) 71, 143, 310, 347
ApoI RAATTY 2 cut(s) 452, 770
AseI ATTAAT 1 cut(s) 936
Asp700I GAANNNNTTC 2 cut(s) 18, 890
AspLEI GCGC 1 cut(s) 655
AspS9I GGNCC 4 cut(s) 170, 379, 380, 604
AsuC2I CCSGG 1 cut(s) 594
AsuHPI GGTGA 4 cut(s) 218, 773, 884, 1033
AvaII GGWCC 1 cut(s) 170
BaeGI GKGCMC 2 cut(s) 383, 401
BalI TGGCCA 1 cut(s) 197
BanII GRGCYC 1 cut(s) 383
BbsI GAAGAC 2 cut(s) 333, 525
Bbv12I GWGCWC 1 cut(s) 401
BbvI GCAGC 4 cut(s) 58, 130, 297, 334
BccI CCATC 2 cut(s) 268, 869
BceAI ACGGC 1 cut(s) 596
BciT130I CCWGG 2 cut(s) 150, 1010
BclI TGATCA 2 cut(s) 481, 799
BcnI CCSGG 1 cut(s) 594
BcoDI GTCTC 2 cut(s) 782, 984
BfaI CTAG 1 cut(s) 1112
BfmI CTRYAG 1 cut(s) 828
BisI GCNGC 4 cut(s) 72, 144, 311, 348
BlsI GCNGC 4 cut(s) 73, 145, 312, 349
Bme1390I CCNGG 3 cut(s) 150, 594, 1010
Bme18I GGWCC 1 cut(s) 170
BmgT120I GGNCC 4 cut(s) 170, 379, 380, 604
BmiI GGNNCC 2 cut(s) 381, 605
BmrFI CCNGG 3 cut(s) 150, 594, 1010
BmsI GCATC 3 cut(s) 58, 454, 1003
BpiI GAAGAC 2 cut(s) 333, 525
BplI GAGNNNNNCTC 4 cut(s) 48, 80, 524, 556
BpmI CTGGAG 1 cut(s) 810
BpuEI CTTGAG 1 cut(s) 612
BpuMI CCSGG 1 cut(s) 594
BsaAI YACGTR 1 cut(s) 220
BsaJI CCNNGG 2 cut(s) 201, 983
Bsc4I CCNNNNNNNGG 2 cut(s) 599, 600
Bse118I RCCGGY 1 cut(s) 556
BseBI CCWGG 2 cut(s) 150, 1010
BseDI CCNNGG 2 cut(s) 201, 983
BseGI GGATG 1 cut(s) 1018
BseLI CCNNNNNNNGG 2 cut(s) 599, 600
BseMII CTCAG 1 cut(s) 109
BseRI GAGGAG 4 cut(s) 24, 320, 393, 894
BseSI GKGCMC 2 cut(s) 383, 401
BseXI GCAGC 4 cut(s) 58, 130, 297, 334
BseYI CCCAGC 1 cut(s) 376
BshFI GGCC 6 cut(s) 93, 197, 235, 269, 381, 606
BsiHKAI GWGCWC 1 cut(s) 401
BsiSI CCGG 3 cut(s) 266, 557, 594
BslFI GGGAC 1 cut(s) 1127
BslI CCNNNNNNNGG 2 cut(s) 599, 600
BsmAI GTCTC 2 cut(s) 782, 984
BsmFI GGGAC 1 cut(s) 1127
BsnI GGCC 6 cut(s) 93, 197, 235, 269, 381, 606
Bsp120I GGGCCC 1 cut(s) 379
Bsp1286I GDGCHC 2 cut(s) 383, 401
Bsp143I GATC 5 cut(s) 177, 430, 481, 799, 901
Bsp19I CCATGG 1 cut(s) 201
BspANI GGCC 6 cut(s) 93, 197, 235, 269, 381, 606
BspCNI CTCAG 1 cut(s) 108
BspLI GGNNCC 2 cut(s) 381, 605
BspPI GGATC 1 cut(s) 438
BsrFI RCCGGY 1 cut(s) 556
BssAI RCCGGY 1 cut(s) 556
BssECI CCNNGG 2 cut(s) 201, 983
BssMI GATC 5 cut(s) 177, 430, 481, 799, 901
BssNAI GTATAC 1 cut(s) 217
BssT1I CCWWGG 1 cut(s) 201
Bst1107I GTATAC 1 cut(s) 217
Bst2UI CCWGG 2 cut(s) 150, 1010
Bst4CI ACNGT 6 cut(s) 251, 297, 572, 672, 1031, 1087
Bst6I CTCTTC 1 cut(s) 501
BstBAI YACGTR 1 cut(s) 220
BstC8I GCNNGC 1 cut(s) 958
BstDEI CTNAG 2 cut(s) 95, 708
BstDSI CCRYGG 1 cut(s) 201
BstF5I GGATG 1 cut(s) 1018
BstHHI GCGC 1 cut(s) 655
BstKTI GATC 5 cut(s) 180, 433, 484, 802, 904
BstMAI GTCTC 2 cut(s) 782, 984
BstMBI GATC 5 cut(s) 177, 430, 481, 799, 901
BstMWI GCNNNNNNNGC 1 cut(s) 316
BstNI CCWGG 2 cut(s) 150, 1010
BstSCI CCNGG 3 cut(s) 148, 592, 1008
BstSFI CTRYAG 1 cut(s) 828
BstSLI GKGCMC 2 cut(s) 383, 401
BstSNI TACGTA 1 cut(s) 220
BstV1I GCAGC 4 cut(s) 58, 130, 297, 334
BstV2I GAAGAC 2 cut(s) 333, 525
BstXI CCANNNNNNTGG 2 cut(s) 193, 447
BstZ17I GTATAC 1 cut(s) 217
BsuRI GGCC 6 cut(s) 93, 197, 235, 269, 381, 606
BtgI CCRYGG 1 cut(s) 201
BtsCI GGATG 1 cut(s) 1018
BtsI GCAGTG 1 cut(s) 542
BtsIMutI CAGTG 1 cut(s) 542
Cac8I GCNNGC 1 cut(s) 958
CfoI GCGC 1 cut(s) 655
Cfr10I RCCGGY 1 cut(s) 556
Cfr13I GGNCC 4 cut(s) 170, 379, 380, 604
Csp6I GTAC 2 cut(s) 252, 1027
CviAII CATG 8 cut(s) 202, 300, 407, 810, 899, 1024, 1122, 1136
CviQI GTAC 2 cut(s) 252, 1027
DdeI CTNAG 2 cut(s) 95, 708
DpnI GATC 5 cut(s) 179, 432, 483, 801, 903
DpnII GATC 5 cut(s) 177, 430, 481, 799, 901
EaeI YGGCCR 2 cut(s) 195, 233
Eam1104I CTCTTC 1 cut(s) 501
EarI CTCTTC 1 cut(s) 501
Eco105I TACGTA 1 cut(s) 220
Eco130I CCWWGG 1 cut(s) 201
Eco24I GRGCYC 1 cut(s) 383
Eco47I GGWCC 1 cut(s) 170
EcoO109I RGGNCCY 1 cut(s) 604
EcoRI GAATTC 1 cut(s) 452
EcoRII CCWGG 2 cut(s) 148, 1008
EcoT14I CCWWGG 1 cut(s) 201
EcoT22I ATGCAT 1 cut(s) 1137
EcoT38I GRGCYC 1 cut(s) 383
ErhI CCWWGG 1 cut(s) 201
FaeI CATG 8 cut(s) 205, 303, 410, 813, 902, 1027, 1125, 1139
FaqI GGGAC 1 cut(s) 1127
FatI CATG 8 cut(s) 201, 299, 406, 809, 898, 1023, 1121, 1135
FbaI TGATCA 2 cut(s) 481, 799
FblI GTMKAC 1 cut(s) 216
Fnu4HI GCNGC 4 cut(s) 72, 144, 311, 348
FokI GGATG 1 cut(s) 1025
FriOI GRGCYC 1 cut(s) 383
Fsp4HI GCNGC 4 cut(s) 72, 144, 311, 348
FspBI CTAG 1 cut(s) 1112
GlaI GCGC 1 cut(s) 654
GluI GCNGC 4 cut(s) 72, 144, 311, 348
GsaI CCCAGC 1 cut(s) 380
GsuI CTGGAG 1 cut(s) 810
HaeIII GGCC 6 cut(s) 93, 197, 235, 269, 381, 606
HapII CCGG 3 cut(s) 266, 557, 594
HhaI GCGC 1 cut(s) 655
Hin1II CATG 8 cut(s) 205, 303, 410, 813, 902, 1027, 1125, 1139
Hin6I GCGC 1 cut(s) 653
HinP1I GCGC 1 cut(s) 653
HindIII AAGCTT 1 cut(s) 710
HinfI GANTC 5 cut(s) 19, 86, 119, 284, 977
HpaII CCGG 3 cut(s) 266, 557, 594
HphI GGTGA 4 cut(s) 218, 773, 884, 1033
Hpy166II GTNNAC 2 cut(s) 217, 399
Hpy188I TCNGA 4 cut(s) 24, 505, 552, 984
Hpy188III TCNNGA 3 cut(s) 65, 677, 789
Hpy8I GTNNAC 2 cut(s) 217, 399
HpyAV CCTTC 2 cut(s) 36, 1027
HpyCH4III ACNGT 6 cut(s) 251, 297, 572, 672, 1031, 1087
HpyCH4IV ACGT 2 cut(s) 219, 426
HpyCH4V TGCA 7 cut(s) 74, 290, 399, 467, 587, 960, 1135
HpyF10VI GCNNNNNNNGC 1 cut(s) 316
HpyF3I CTNAG 2 cut(s) 95, 708
HpySE526I ACGT 2 cut(s) 219, 426
Hsp92II CATG 8 cut(s) 205, 303, 410, 813, 902, 1027, 1125, 1139
HspAI GCGC 1 cut(s) 653
Ksp22I TGATCA 2 cut(s) 481, 799
Kzo9I GATC 5 cut(s) 177, 430, 481, 799, 901
LmnI GCTCC 3 cut(s) 151, 307, 492
Lsp1109I GCAGC 4 cut(s) 58, 130, 297, 334
LweI GCATC 3 cut(s) 58, 454, 1003
MaeI CTAG 1 cut(s) 1112
MaeII ACGT 2 cut(s) 219, 426
MaeIII GTNAC 4 cut(s) 206, 419, 641, 1031
MalI GATC 5 cut(s) 179, 432, 483, 801, 903
MboI GATC 5 cut(s) 177, 430, 481, 799, 901
MboII GAAGA 6 cut(s) 37, 333, 473, 518, 530, 896
MhlI GDGCHC 2 cut(s) 383, 401
MlsI TGGCCA 1 cut(s) 197
MluCI AATT 8 cut(s) 325, 452, 499, 770, 795, 859, 937, 1003
MluNI TGGCCA 1 cut(s) 197
MlyI GAGTC 2 cut(s) 80, 986
MmeI TCCRAC 1 cut(s) 150
Mox20I TGGCCA 1 cut(s) 197
Mph1103I ATGCAT 1 cut(s) 1137
MroXI GAANNNNTTC 2 cut(s) 18, 890
MscI TGGCCA 1 cut(s) 197
MseI TTAA 2 cut(s) 936, 1002
MslI CAYNNNNRTG 3 cut(s) 445, 814, 870
Msp20I TGGCCA 1 cut(s) 197
MspI CCGG 3 cut(s) 266, 557, 594
MspR9I CCNGG 3 cut(s) 150, 594, 1010
MvaI CCWGG 2 cut(s) 150, 1010
MwoI GCNNNNNNNGC 1 cut(s) 316
NciI CCSGG 1 cut(s) 594
NcoI CCATGG 1 cut(s) 201
NdeII GATC 5 cut(s) 177, 430, 481, 799, 901
NlaIII CATG 8 cut(s) 205, 303, 410, 813, 902, 1027, 1125, 1139
NlaIV GGNNCC 2 cut(s) 381, 605
NmuCI GTSAC 1 cut(s) 206
NsiI ATGCAT 1 cut(s) 1137
OliI CACNNNNGTG 1 cut(s) 870
PdmI GAANNNNTTC 2 cut(s) 18, 890
PfeI GAWTC 3 cut(s) 19, 119, 284
PfoI TCCNGGA 1 cut(s) 1008
PkrI GCNGC 4 cut(s) 73, 145, 312, 349
PleI GAGTC 2 cut(s) 80, 985
PpsI GAGTC 2 cut(s) 80, 985
Ppu21I YACGTR 1 cut(s) 220
PshBI ATTAAT 1 cut(s) 936
Psp6I CCWGG 2 cut(s) 148, 1008
PspFI CCCAGC 1 cut(s) 376
PspGI CCWGG 2 cut(s) 148, 1008
PspN4I GGNNCC 2 cut(s) 381, 605
PspOMI GGGCCC 1 cut(s) 379
PspPI GGNCC 4 cut(s) 170, 379, 380, 604
RsaI GTAC 2 cut(s) 253, 1028
RsaNI GTAC 2 cut(s) 252, 1027
RseI CAYNNNNRTG 3 cut(s) 445, 814, 870
SaqAI TTAA 2 cut(s) 936, 1002
SatI GCNGC 4 cut(s) 72, 144, 311, 348
Sau3AI GATC 5 cut(s) 177, 430, 481, 799, 901
Sau96I GGNCC 4 cut(s) 170, 379, 380, 604
SchI GAGTC 2 cut(s) 80, 986
ScrFI CCNGG 3 cut(s) 150, 594, 1010
SduI GDGCHC 2 cut(s) 383, 401
SfaNI GCATC 3 cut(s) 58, 454, 1003
SfcI CTRYAG 1 cut(s) 828
SinI GGWCC 1 cut(s) 170
SmiMI CAYNNNNRTG 3 cut(s) 445, 814, 870
SmlI CTYRAG 1 cut(s) 627
SmoI CTYRAG 1 cut(s) 627
SnaBI TACGTA 1 cut(s) 220
Sse9I AATT 8 cut(s) 325, 452, 499, 770, 795, 859, 937, 1003
SspI AATATT 1 cut(s) 934
SspMI CTAG 1 cut(s) 1112
StyD4I CCNGG 3 cut(s) 148, 592, 1008
StyI CCWWGG 1 cut(s) 201
TaaI ACNGT 6 cut(s) 251, 297, 572, 672, 1031, 1087
TaiI ACGT 2 cut(s) 222, 429
TaqI TCGA 1 cut(s) 66
TasI AATT 8 cut(s) 325, 452, 499, 770, 795, 859, 937, 1003
TfiI GAWTC 3 cut(s) 19, 119, 284
Tru1I TTAA 2 cut(s) 936, 1002
Tru9I TTAA 2 cut(s) 936, 1002
TscAI CASTG 1 cut(s) 549
TseFI GTSAC 1 cut(s) 206
TseI GCWGC 4 cut(s) 71, 143, 310, 347
Tsp45I GTSAC 1 cut(s) 206
TspDTI ATGAA 5 cut(s) 45, 356, 445, 798, 1110
TspGWI ACGGA 2 cut(s) 144, 1093
TspRI CASTG 1 cut(s) 549
VneI GTGCAC 1 cut(s) 397
VpaK11BI GGWCC 1 cut(s) 170
VspI ATTAAT 1 cut(s) 936
XapI RAATTY 2 cut(s) 452, 770
XmiI GTMKAC 1 cut(s) 216
XmnI GAANNNNTTC 2 cut(s) 18, 890
XspI CTAG 1 cut(s) 1112
Zsp2I ATGCAT 1 cut(s) 1137
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.