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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AcsI | RAATTY | 1 cut(s) | 260 |
| AfaI | GTAC | 2 cut(s) | 29, 179 |
| AgsI | TTSAA | 1 cut(s) | 104 |
| AhlI | ACTAGT | 1 cut(s) | 106 |
| AluBI | AGCT | 2 cut(s) | 73, 218 |
| AluI | AGCT | 2 cut(s) | 73, 218 |
| Ama87I | CYCGRG | 1 cut(s) | 74 |
| AoxI | GGCC | 1 cut(s) | 274 |
| ApeKI | GCWGC | 1 cut(s) | 251 |
| ApoI | RAATTY | 1 cut(s) | 260 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 198 |
| AsuHPI | GGTGA | 1 cut(s) | 70 |
| AsuII | TTCGAA | 1 cut(s) | 167 |
| AvaI | CYCGRG | 1 cut(s) | 74 |
| BbvI | GCAGC | 1 cut(s) | 238 |
| BcuI | ACTAGT | 1 cut(s) | 106 |
| BfaI | CTAG | 2 cut(s) | 107, 299 |
| BisI | GCNGC | 1 cut(s) | 252 |
| BlsI | GCNGC | 1 cut(s) | 253 |
| BmeT110I | CYCGRG | 1 cut(s) | 74 |
| BpmI | CTGGAG | 1 cut(s) | 143 |
| Bpu14I | TTCGAA | 1 cut(s) | 167 |
| Bse1I | ACTGG | 1 cut(s) | 286 |
| BseMII | CTCAG | 1 cut(s) | 87 |
| BseNI | ACTGG | 1 cut(s) | 286 |
| BseRI | GAGGAG | 3 cut(s) | 92, 140, 204 |
| BseXI | GCAGC | 1 cut(s) | 238 |
| BshFI | GGCC | 1 cut(s) | 276 |
| BsiHKCI | CYCGRG | 1 cut(s) | 74 |
| BsnI | GGCC | 1 cut(s) | 276 |
| BsoBI | CYCGRG | 1 cut(s) | 74 |
| Bsp119I | TTCGAA | 1 cut(s) | 167 |
| Bsp1407I | TGTACA | 1 cut(s) | 177 |
| BspANI | GGCC | 1 cut(s) | 276 |
| BspCNI | CTCAG | 1 cut(s) | 88 |
| BspT104I | TTCGAA | 1 cut(s) | 167 |
| BsrGI | TGTACA | 1 cut(s) | 177 |
| BsrI | ACTGG | 1 cut(s) | 286 |
| BstAUI | TGTACA | 1 cut(s) | 177 |
| BstBI | TTCGAA | 1 cut(s) | 167 |
| BstDEI | CTNAG | 2 cut(s) | 50, 96 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 79 |
| BstV1I | GCAGC | 1 cut(s) | 238 |
| BsuRI | GGCC | 1 cut(s) | 276 |
| BtsIMutI | CAGTG | 1 cut(s) | 279 |
| Csp6I | GTAC | 2 cut(s) | 28, 178 |
| CviAII | CATG | 1 cut(s) | 181 |
| CviJI | RGCY | 3 cut(s) | 73, 218, 276 |
| CviKI_1 | RGCY | 3 cut(s) | 73, 218, 276 |
| CviQI | GTAC | 2 cut(s) | 28, 178 |
| DdeI | CTNAG | 2 cut(s) | 50, 96 |
| Eco88I | CYCGRG | 1 cut(s) | 74 |
| FaeI | CATG | 1 cut(s) | 184 |
| FaiI | YATR | 2 cut(s) | 141, 182 |
| FatI | CATG | 1 cut(s) | 180 |
| Fnu4HI | GCNGC | 1 cut(s) | 252 |
| Fsp4HI | GCNGC | 1 cut(s) | 252 |
| FspBI | CTAG | 2 cut(s) | 107, 299 |
| GluI | GCNGC | 1 cut(s) | 252 |
| GsuI | CTGGAG | 1 cut(s) | 143 |
| HaeIII | GGCC | 1 cut(s) | 276 |
| Hin1II | CATG | 1 cut(s) | 184 |
| HinfI | GANTC | 5 cut(s) | 61, 89, 164, 199, 302 |
| HphI | GGTGA | 1 cut(s) | 70 |
| Hpy188I | TCNGA | 1 cut(s) | 204 |
| Hpy188III | TCNNGA | 2 cut(s) | 95, 122 |
| HpyAV | CCTTC | 3 cut(s) | 4, 48, 216 |
| HpyCH4V | TGCA | 3 cut(s) | 34, 160, 254 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 79 |
| HpyF3I | CTNAG | 2 cut(s) | 50, 96 |
| Hsp92II | CATG | 1 cut(s) | 184 |
| LmnI | GCTCC | 1 cut(s) | 79 |
| LpnPI | CCDG | 5 cut(s) | 107, 108, 204, 258, 267 |
| Lsp1109I | GCAGC | 1 cut(s) | 238 |
| MaeI | CTAG | 2 cut(s) | 107, 299 |
| MboII | GAAGA | 1 cut(s) | 217 |
| MluCI | AATT | 3 cut(s) | 35, 173, 260 |
| MmeI | TCCRAC | 1 cut(s) | 166 |
| MnlI | CCTC | 5 cut(s) | 70, 118, 182, 280, 287 |
| MroXI | GAANNNNTTC | 1 cut(s) | 198 |
| MseI | TTAA | 2 cut(s) | 39, 66 |
| MspA1I | CMGCKG | 1 cut(s) | 218 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 79 |
| NlaIII | CATG | 1 cut(s) | 184 |
| NspV | TTCGAA | 1 cut(s) | 167 |
| PaeR7I | CTCGAG | 1 cut(s) | 74 |
| PdmI | GAANNNNTTC | 1 cut(s) | 198 |
| PfeI | GAWTC | 5 cut(s) | 61, 89, 164, 199, 302 |
| PkrI | GCNGC | 1 cut(s) | 253 |
| PspXI | VCTCGAGB | 1 cut(s) | 74 |
| PvuII | CAGCTG | 1 cut(s) | 218 |
| RsaI | GTAC | 2 cut(s) | 29, 179 |
| RsaNI | GTAC | 2 cut(s) | 28, 178 |
| SaqAI | TTAA | 2 cut(s) | 39, 66 |
| SatI | GCNGC | 1 cut(s) | 252 |
| SetI | ASST | 4 cut(s) | 15, 75, 220, 227 |
| Sfr274I | CTCGAG | 1 cut(s) | 74 |
| SfuI | TTCGAA | 1 cut(s) | 167 |
| SlaI | CTCGAG | 1 cut(s) | 74 |
| SmlI | CTYRAG | 1 cut(s) | 74 |
| SmoI | CTYRAG | 1 cut(s) | 74 |
| SpeI | ACTAGT | 1 cut(s) | 106 |
| Sse9I | AATT | 3 cut(s) | 35, 173, 260 |
| SspMI | CTAG | 2 cut(s) | 107, 299 |
| TaqI | TCGA | 3 cut(s) | 15, 75, 167 |
| TasI | AATT | 3 cut(s) | 35, 173, 260 |
| TatI | WGTACW | 2 cut(s) | 27, 177 |
| TfiI | GAWTC | 5 cut(s) | 61, 89, 164, 199, 302 |
| Tru1I | TTAA | 2 cut(s) | 39, 66 |
| Tru9I | TTAA | 2 cut(s) | 39, 66 |
| TscAI | CASTG | 1 cut(s) | 286 |
| TseI | GCWGC | 1 cut(s) | 251 |
| TspDTI | ATGAA | 1 cut(s) | 225 |
| TspRI | CASTG | 1 cut(s) | 286 |
| XapI | RAATTY | 1 cut(s) | 260 |
| XhoI | CTCGAG | 1 cut(s) | 74 |
| XmnI | GAANNNNTTC | 1 cut(s) | 198 |
| XspI | CTAG | 2 cut(s) | 107, 299 |