FvH4_2g26650

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0000778
Physical Location & Seq
Forward (+)
21366594 .. 21368560
1967 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g26650.t2

Sequence Viewer

Length: 1458 bp
ATGGATTACAACTTTTACATTCTCTTGTCTACACTACTTACCATCTTCACTACCATCACTCTTGCATTCCTTGTTCGTGCTTACAAATCAAAACTCCATGAACAATCCATCAATCTTCCCCCAGGCAGTTTTGGGTGGCCGATTATCGGCGAAACCTTTGCCTTCCTACAGAGTGACCACAACAAATTTGTAGGGGATAGAATGAAGAAATACTCCTCCAAAATCTTCAAAACCAAGATTCTAGGTGAGCGAACCGTCGTCTTATGTGGTACTGCCGGTCACAAATTTGTGGCTGCCAACGAAGAAAAGCTCTTTGTAGCATGGAGACCACACTCCATGCAGAAGCTCTTTCGCTCTTCTTACCAGAAAGCTGCTTCCACCGTCATACCAAGACAAAGCGAAAAGCATGTAGTTACCGCACCGGGGTTTTTGAAAGCCGAAGCCCTTGTTCGCTACGTGGGAGGGATGGATTCCATGGTTCTTGACCACCTGAAAAAGCACTGGGATGGCAAACAGGTGCTCGAGGCTCATCGCCTCACGCAGCAATTAGTTTTGTCCCTCTCGGCCAAGTTCTTCATGGGACTTGAGGACCAGTCTCGTATTGAGAAGCTTGCGGAGTTGATGGACACCATGATGCTGGCGCTTCATACTATTCCTGTGAATATTCCGGGGACTACGTTTTATAAAGCAATGAAAGCATCGCAGGCTGTGAGGGAGACTATTATGTCGTTCATTAAGGAGAAGAAAGAAATTATGGCCTCTACCGGAAACAAAGTGCAAGATCTTTTGTCTTATATGATTTCTACGGCAGACCCCTTTACCGGAAAGTTGATGCCGGACAATGAAATTGCGGATAAAATTATGGGGAATGTGGCCGCTTCGTTCAACTCTCCGGCTATGACTACTACTTTTATCGTCAAGTTTCTGGGCGAAAGACCTGACATTTATGAGAAAGTTCGAAACGAACAACTGGAGATTTTAAAGTCCAAAAAATTTGGCGAGGCACTCAATTGGGATGATTTGAACAAAATGAAGTATTCGTGGAACGTAGCCCTGGAGGCGATGCGGCTCATACCACCTCTTCAGGGAACATTCAGAGAGGCTGCAACAGATATCACTTACCAAGGTTATACAATTCCTAAGGGATGGAAGGTCTACTGGACAGTGAGCACCACAGCAATGAACCCTGAATATTTTCCAGCGCCAGAAGAGTTTGATCCTACAAGGTTCGAGAAATCAAGTCCTCCGCCTTACACAAACATCCCATTCGGGAGTGGTCCTCGAATTTGCCCCGGAAAGGACTACTCTCGCCTGCAAATTCTTAGTTTTATGCACCATCTTGTTATAAGATTCAAATGGGAACTTGTAAACCCTGATTGCAAGATCACAGGTGGTATGAATCCCTTTCCAGTTGATGGCCTGATGATCCGCCTCCAAGCTCAACCCGTTTCAGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
p450

Protein Analysis

486

Amino Acids

55.04

Weight (kDa)

9.28

Isoelectric Point (pI)

39.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 40 - 387 4e-34 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 684, 1346
AasI GACNNNNNNGTC 1 cut(s) 724
AccB7I CCANNNNNTGG 1 cut(s) 1415
AccI GTMKAC 2 cut(s) 29, 1155
AciI CCGC 7 cut(s) 417, 614, 851, 876, 1066, 1247, 1429
AclWI GGATC 2 cut(s) 1211, 1420
AcoI YGGCCR 3 cut(s) 137, 564, 873
AcsI RAATTY 5 cut(s) 185, 284, 992, 1284, 1317
AcuI CTGAAG 1 cut(s) 1067
AfaI GTAC 1 cut(s) 271
AfiI CCNNNNNNNGG 6 cut(s) 146, 423, 821, 1085, 1297, 1415
AgsI TTSAA 5 cut(s) 229, 433, 886, 1024, 1354
AjnI CCWGG 2 cut(s) 121, 1053
AluBI AGCT 6 cut(s) 310, 346, 371, 610, 1439, 1454
AluI AGCT 6 cut(s) 310, 346, 371, 610, 1439, 1454
Alw21I GWGCWC 2 cut(s) 522, 1172
Alw26I GTCTC 3 cut(s) 319, 600, 710
AlwI GGATC 2 cut(s) 1211, 1420
Ama87I CYCGRG 1 cut(s) 521
AoxI GGCC 5 cut(s) 137, 564, 756, 873, 1417
ApeKI GCWGC 4 cut(s) 293, 371, 541, 1103
ApoI RAATTY 5 cut(s) 185, 284, 992, 1284, 1317
Asp700I GAANNNNTTC 1 cut(s) 1194
AspLEI GCGC 2 cut(s) 643, 1204
AspS9I GGNCC 2 cut(s) 589, 1277
AsuC2I CCSGG 3 cut(s) 423, 669, 1293
AsuHPI GGTGA 1 cut(s) 257
AsuII TTCGAA 1 cut(s) 958
AvaI CYCGRG 1 cut(s) 521
AvaII GGWCC 2 cut(s) 589, 1277
AxyI CCTNAGG 1 cut(s) 1140
Bbv12I GWGCWC 2 cut(s) 522, 1172
BbvI GCAGC 4 cut(s) 280, 358, 553, 1090
BccI CCATC 9 cut(s) 50, 62, 116, 460, 500, 616, 1140, 1344, 1409
BceAI ACGGC 1 cut(s) 822
BcgI CGANNNNNNTGC 2 cut(s) 140, 174
BciT130I CCWGG 2 cut(s) 123, 1055
BcnI CCSGG 3 cut(s) 423, 669, 1293
BcoDI GTCTC 3 cut(s) 319, 600, 710
BfaI CTAG 1 cut(s) 242
BfmI CTRYAG 1 cut(s) 167
BfoI RGCGCY 2 cut(s) 644, 1205
BglI GCCNNNNNGGC 1 cut(s) 1058
BglII AGATCT 1 cut(s) 781
BisI GCNGC 6 cut(s) 294, 372, 542, 876, 1067, 1104
BlsI GCNGC 6 cut(s) 295, 373, 543, 877, 1068, 1105
Bme1390I CCNGG 5 cut(s) 123, 423, 669, 1055, 1293
Bme18I GGWCC 2 cut(s) 589, 1277
BmeT110I CYCGRG 1 cut(s) 521
BmgT120I GGNCC 2 cut(s) 589, 1277
BmrFI CCNGG 5 cut(s) 123, 423, 669, 1055, 1293
BmrI ACTGGG 1 cut(s) 511
BmsI GCATC 4 cut(s) 624, 707, 822, 1053
BmuI ACTGGG 1 cut(s) 511
BplI GAGNNNNNCTC 2 cut(s) 1264, 1296
BpmI CTGGAG 2 cut(s) 992, 1076
Bpu14I TTCGAA 1 cut(s) 958
BpuEI CTTGAG 1 cut(s) 605
BpuMI CCSGG 3 cut(s) 423, 669, 1293
BsaAI YACGTR 1 cut(s) 457
BsaI GGTCTC 1 cut(s) 319
BsaJI CCNNGG 7 cut(s) 121, 422, 474, 668, 1053, 1123, 1291
BsaWI WCCGGW 2 cut(s) 764, 821
Bsc4I CCNNNNNNNGG 6 cut(s) 146, 423, 821, 1085, 1297, 1415
Bse118I RCCGGY 1 cut(s) 275
Bse1I ACTGG 5 cut(s) 506, 592, 975, 1163, 1409
Bse21I CCTNAGG 1 cut(s) 1140
Bse3DI GCAATG 2 cut(s) 696, 1185
BseBI CCWGG 2 cut(s) 123, 1055
BseDI CCNNGG 7 cut(s) 121, 422, 474, 668, 1053, 1123, 1291
BseGI GGATG 5 cut(s) 471, 511, 1021, 1151, 1260
BseLI CCNNNNNNNGG 6 cut(s) 146, 423, 821, 1085, 1297, 1415
BseMI GCAATG 2 cut(s) 696, 1185
BseNI ACTGG 5 cut(s) 506, 592, 975, 1163, 1409
BseRI GAGGAG 1 cut(s) 205
BseXI GCAGC 4 cut(s) 280, 358, 553, 1090
BshFI GGCC 5 cut(s) 139, 566, 758, 875, 1419
BsiHKAI GWGCWC 2 cut(s) 522, 1172
BsiHKCI CYCGRG 1 cut(s) 521
BsiSI CCGG 8 cut(s) 276, 422, 668, 765, 822, 836, 893, 1293
BslFI GGGAC 3 cut(s) 541, 594, 685
BslI CCNNNNNNNGG 6 cut(s) 146, 423, 821, 1085, 1297, 1415
BsmAI GTCTC 3 cut(s) 319, 600, 710
BsmFI GGGAC 3 cut(s) 541, 594, 685
BsmI GAATGC 1 cut(s) 65
BsnI GGCC 5 cut(s) 139, 566, 758, 875, 1419
Bso31I GGTCTC 1 cut(s) 319
BsoBI CYCGRG 1 cut(s) 521
Bsp119I TTCGAA 1 cut(s) 958
Bsp1286I GDGCHC 2 cut(s) 522, 1172
Bsp143I GATC 4 cut(s) 781, 1216, 1383, 1425
Bsp19I CCATGG 1 cut(s) 474
BspACI CCGC 7 cut(s) 417, 614, 851, 876, 1066, 1247, 1429
BspANI GGCC 5 cut(s) 139, 566, 758, 875, 1419
BspPI GGATC 2 cut(s) 1211, 1420
BspQI GCTCTTC 1 cut(s) 361
BspT104I TTCGAA 1 cut(s) 958
BspTNI GGTCTC 1 cut(s) 319
BsrDI GCAATG 2 cut(s) 696, 1185
BsrFI RCCGGY 1 cut(s) 275
BsrI ACTGG 5 cut(s) 506, 592, 975, 1163, 1409
BssAI RCCGGY 1 cut(s) 275
BssECI CCNNGG 7 cut(s) 121, 422, 474, 668, 1053, 1123, 1291
BssMI GATC 4 cut(s) 781, 1216, 1383, 1425
BssT1I CCWWGG 2 cut(s) 474, 1123
Bst2UI CCWGG 2 cut(s) 123, 1055
Bst4CI ACNGT 3 cut(s) 256, 382, 1165
Bst6I CTCTTC 3 cut(s) 361, 1086, 1203
BstBAI YACGTR 1 cut(s) 457
BstBI TTCGAA 1 cut(s) 958
BstC8I GCNNGC 4 cut(s) 612, 639, 705, 1313
BstDEI CTNAG 2 cut(s) 1140, 1322
BstDSI CCRYGG 1 cut(s) 474
BstF5I GGATG 5 cut(s) 471, 511, 1021, 1151, 1260
BstH2I RGCGCY 2 cut(s) 644, 1205
BstHHI GCGC 2 cut(s) 643, 1204
BstKTI GATC 4 cut(s) 784, 1219, 1386, 1428
BstMAI GTCTC 3 cut(s) 319, 600, 710
BstMBI GATC 4 cut(s) 781, 1216, 1383, 1425
BstMWI GCNNNNNNNGC 3 cut(s) 695, 704, 1058
BstNI CCWGG 2 cut(s) 123, 1055
BstNSI RCATGY 1 cut(s) 410
BstSCI CCNGG 5 cut(s) 121, 421, 667, 1053, 1291
BstSFI CTRYAG 1 cut(s) 167
BstV1I GCAGC 4 cut(s) 280, 358, 553, 1090
BstX2I RGATCY 1 cut(s) 781
BstXI CCANNNNNNTGG 1 cut(s) 637
BstYI RGATCY 1 cut(s) 781
Bsu36I CCTNAGG 1 cut(s) 1140
BsuRI GGCC 5 cut(s) 139, 566, 758, 875, 1419
BtgI CCRYGG 1 cut(s) 474
BtgZI GCGATG 3 cut(s) 515, 684, 1076
BtsCI GGATG 5 cut(s) 471, 511, 1021, 1151, 1260
BtsIMutI CAGTG 2 cut(s) 499, 1170
Cac8I GCNNGC 4 cut(s) 612, 639, 705, 1313
CfoI GCGC 2 cut(s) 643, 1204
Cfr10I RCCGGY 1 cut(s) 275
Cfr13I GGNCC 2 cut(s) 589, 1277
Csp6I GTAC 1 cut(s) 270
CviAII CATG 7 cut(s) 98, 321, 337, 407, 475, 577, 631
CviQI GTAC 1 cut(s) 270
DdeI CTNAG 2 cut(s) 1140, 1322
DpnI GATC 4 cut(s) 783, 1218, 1385, 1427
DpnII GATC 4 cut(s) 781, 1216, 1383, 1425
DraI TTTAAA 1 cut(s) 981
DrdI GACNNNNNNGTC 1 cut(s) 724
DseDI GACNNNNNNGTC 1 cut(s) 724
EaeI YGGCCR 3 cut(s) 137, 564, 873
Eam1104I CTCTTC 3 cut(s) 361, 1086, 1203
EarI CTCTTC 3 cut(s) 361, 1086, 1203
EciI GGCGGA 2 cut(s) 1236, 1418
Eco130I CCWWGG 2 cut(s) 474, 1123
Eco31I GGTCTC 1 cut(s) 319
Eco32I GATATC 1 cut(s) 1114
Eco47I GGWCC 2 cut(s) 589, 1277
Eco57I CTGAAG 1 cut(s) 1067
Eco81I CCTNAGG 1 cut(s) 1140
Eco88I CYCGRG 1 cut(s) 521
EcoRII CCWGG 2 cut(s) 121, 1053
EcoRV GATATC 1 cut(s) 1114
EcoT14I CCWWGG 2 cut(s) 474, 1123
ErhI CCWWGG 2 cut(s) 474, 1123
FaeI CATG 7 cut(s) 101, 324, 340, 410, 478, 580, 634
FaqI GGGAC 3 cut(s) 541, 594, 685
FatI CATG 7 cut(s) 97, 320, 336, 406, 474, 576, 630
FblI GTMKAC 2 cut(s) 29, 1155
Fnu4HI GCNGC 6 cut(s) 294, 372, 542, 876, 1067, 1104
FokI GGATG 5 cut(s) 478, 518, 1028, 1158, 1247
Fsp4HI GCNGC 6 cut(s) 294, 372, 542, 876, 1067, 1104
FspBI CTAG 1 cut(s) 242
GlaI GCGC 2 cut(s) 642, 1203
GluI GCNGC 6 cut(s) 294, 372, 542, 876, 1067, 1104
GsuI CTGGAG 2 cut(s) 992, 1076
HaeII RGCGCY 2 cut(s) 644, 1205
HaeIII GGCC 5 cut(s) 139, 566, 758, 875, 1419
HapII CCGG 8 cut(s) 276, 422, 668, 765, 822, 836, 893, 1293
HhaI GCGC 2 cut(s) 643, 1204
Hin1II CATG 7 cut(s) 101, 324, 340, 410, 478, 580, 634
Hin6I GCGC 2 cut(s) 641, 1202
HinP1I GCGC 2 cut(s) 641, 1202
HindIII AAGCTT 1 cut(s) 608
HinfI GANTC 4 cut(s) 238, 470, 1350, 1399
HpaII CCGG 8 cut(s) 276, 422, 668, 765, 822, 836, 893, 1293
HphI GGTGA 1 cut(s) 257
Hpy166II GTNNAC 3 cut(s) 30, 1156, 1369
Hpy188I TCNGA 1 cut(s) 1097
Hpy188III TCNNGA 3 cut(s) 482, 1231, 1270
Hpy8I GTNNAC 3 cut(s) 30, 1156, 1369
Hpy99I CGWCG 1 cut(s) 260
HpyAV CCTTC 2 cut(s) 172, 1144
HpyCH4III ACNGT 3 cut(s) 256, 382, 1165
HpyCH4IV ACGT 3 cut(s) 456, 677, 1047
HpyCH4V TGCA 7 cut(s) 65, 340, 778, 1106, 1315, 1333, 1380
HpyF10VI GCNNNNNNNGC 3 cut(s) 695, 704, 1058
HpyF3I CTNAG 2 cut(s) 1140, 1322
HpySE526I ACGT 3 cut(s) 456, 677, 1047
Hsp92II CATG 7 cut(s) 101, 324, 340, 410, 478, 580, 634
HspAI GCGC 2 cut(s) 641, 1202
Kzo9I GATC 4 cut(s) 781, 1216, 1383, 1425
LguI GCTCTTC 1 cut(s) 361
Lsp1109I GCAGC 4 cut(s) 280, 358, 553, 1090
LweI GCATC 4 cut(s) 624, 707, 822, 1053
MaeI CTAG 1 cut(s) 242
MaeII ACGT 3 cut(s) 456, 677, 1047
MaeIII GTNAC 3 cut(s) 173, 278, 412
MalI GATC 4 cut(s) 783, 1218, 1385, 1427
MboI GATC 4 cut(s) 781, 1216, 1383, 1425
MfeI CAATTG 1 cut(s) 1009
MflI RGATCY 1 cut(s) 781
MhlI GDGCHC 2 cut(s) 522, 1172
MroXI GAANNNNTTC 1 cut(s) 1194
MseI TTAA 3 cut(s) 735, 980, 1456
MslI CAYNNNNRTG 3 cut(s) 504, 632, 1178
MspI CCGG 8 cut(s) 276, 422, 668, 765, 822, 836, 893, 1293
MspR9I CCNGG 5 cut(s) 123, 423, 669, 1055, 1293
MunI CAATTG 1 cut(s) 1009
Mva1269I GAATGC 1 cut(s) 65
MvaI CCWGG 2 cut(s) 123, 1055
MwoI GCNNNNNNNGC 3 cut(s) 695, 704, 1058
NciI CCSGG 3 cut(s) 423, 669, 1293
NcoI CCATGG 1 cut(s) 474
NdeII GATC 4 cut(s) 781, 1216, 1383, 1425
NlaIII CATG 7 cut(s) 101, 324, 340, 410, 478, 580, 634
NmeAIII GCCGAG 1 cut(s) 542
NmuCI GTSAC 2 cut(s) 173, 278
NspI RCATGY 1 cut(s) 410
NspV TTCGAA 1 cut(s) 958
PaeR7I CTCGAG 1 cut(s) 521
PciSI GCTCTTC 1 cut(s) 361
PctI GAATGC 1 cut(s) 65
PdmI GAANNNNTTC 1 cut(s) 1194
PfeI GAWTC 4 cut(s) 238, 470, 1350, 1399
PflMI CCANNNNNTGG 1 cut(s) 1415
PkrI GCNGC 6 cut(s) 295, 373, 543, 877, 1068, 1105
Ppu21I YACGTR 1 cut(s) 457
PsiI TTATAA 2 cut(s) 684, 1346
Psp6I CCWGG 2 cut(s) 121, 1053
PspGI CCWGG 2 cut(s) 121, 1053
PspPI GGNCC 2 cut(s) 589, 1277
PspXI VCTCGAGB 1 cut(s) 521
PsuI RGATCY 1 cut(s) 781
RsaI GTAC 1 cut(s) 271
RsaNI GTAC 1 cut(s) 270
RseI CAYNNNNRTG 3 cut(s) 504, 632, 1178
SapI GCTCTTC 1 cut(s) 361
SaqAI TTAA 3 cut(s) 735, 980, 1456
SatI GCNGC 6 cut(s) 294, 372, 542, 876, 1067, 1104
Sau3AI GATC 4 cut(s) 781, 1216, 1383, 1425
Sau96I GGNCC 2 cut(s) 589, 1277
ScrFI CCNGG 5 cut(s) 123, 423, 669, 1055, 1293
SduI GDGCHC 2 cut(s) 522, 1172
SfaNI GCATC 4 cut(s) 624, 707, 822, 1053
SfcI CTRYAG 1 cut(s) 167
Sfr274I CTCGAG 1 cut(s) 521
SfuI TTCGAA 1 cut(s) 958
SinI GGWCC 2 cut(s) 589, 1277
SlaI CTCGAG 1 cut(s) 521
SmiMI CAYNNNNRTG 3 cut(s) 504, 632, 1178
SmlI CTYRAG 2 cut(s) 521, 584
SmoI CTYRAG 2 cut(s) 521, 584
SsiI CCGC 7 cut(s) 417, 614, 851, 876, 1066, 1247, 1429
SspI AATATT 2 cut(s) 664, 1193
SspMI CTAG 1 cut(s) 242
StyD4I CCNGG 5 cut(s) 121, 421, 667, 1053, 1291
StyI CCWWGG 2 cut(s) 474, 1123
TaaI ACNGT 3 cut(s) 256, 382, 1165
TaiI ACGT 3 cut(s) 459, 680, 1050
TaqI TCGA 4 cut(s) 522, 958, 1230, 1282
TauI GCSGC 2 cut(s) 878, 1069
TfiI GAWTC 4 cut(s) 238, 470, 1350, 1399
Tru1I TTAA 3 cut(s) 735, 980, 1456
Tru9I TTAA 3 cut(s) 735, 980, 1456
TscAI CASTG 2 cut(s) 506, 1170
TseFI GTSAC 2 cut(s) 173, 278
TseI GCWGC 4 cut(s) 293, 371, 541, 1103
Tsp45I GTSAC 2 cut(s) 173, 278
TspRI CASTG 2 cut(s) 506, 1170
Van91I CCANNNNNTGG 1 cut(s) 1415
VpaK11BI GGWCC 2 cut(s) 589, 1277
XapI RAATTY 5 cut(s) 185, 284, 992, 1284, 1317
XceI RCATGY 1 cut(s) 410
XcmI CCANNNNNNNNNTGG 1 cut(s) 574
XhoI CTCGAG 1 cut(s) 521
XmiI GTMKAC 2 cut(s) 29, 1155
XmnI GAANNNNTTC 1 cut(s) 1194
XspI CTAG 1 cut(s) 242
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.