Rroxscaffold_5G00375250

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0000778
Physical Location & Seq
Forward (+)
56113726 .. 56115424
1699 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00375250.1

Sequence Viewer

Length: 1365 bp
ATGGAGATAGAGAACCTCTTCCTTCCTCTATCCATCATCGTTTCCGTATATACTCTCGCCTTCATCTTTTACACTATCAGGTCCAAGTCCAACTCCAAAATGAATCTTCCCCCAGGAAGTTTCGGGTGGCCAATCATCGGTGAAACTTTCGCCTTTCTGAATGATGCGCAGGAGAAGTTCGTGGGCGACAGAAAGAAGAAATACTCTCCCAAAGTCTTCAAAACCAGCATACTCGGTGAGCCAACTGCCGTGCTTTGCGGCATTTCCGGTCACAAGTTCATCTCTTCCAACGAGGAAAAACTGTTTCTGTCATGGCGACCGCAGTCCACACAGAAACTCTTCCGCTCCTCCTATCAAAAAGCTGCTTCCGAGATCACCAAACGAGCAGATTCAGTCCATATCGGTAAAGCACCAGGGTTTCTTAAACCTGAAGCGGTGGTTCGGTACATAGAGAGCATGGAGTCCATAGTTCAGGAGCATTTGAAGGTTTACTGGGAAGGCAAAAGCACAGTTGAGATTTACAGCCTCGCTCAGCTACTTGTAACGACTCTGTCCGCTCGATTCTTCACTGGGCTCCAAAACAATAACGACCGTTGTGTTAAGTTTGCCGAGTTGATTAATATCATAGCCTCAGGGCTGCATACGTTCCCAATTAATATTCCAGGAACCGAGTTTTATCGAGCGATGAAAGCAGCCGAGGCTATGAGGAAGGAGGTTCTGTTGTTGATCAAGGAGAAGAAAGCGGCCGTGTCTTCCGGCGGAGCCAAAGTAACCGACATCATGTCTCATTTGCTGTTTAACCCGGATCCGACTGGACGGTTCATACCGGAGAATGAAGTTGCAGATAAGGTCATGGGTTTGATGACGGGTGGATTTCACTCCCCTTCCATGGCTACAAGTTTTCTCGTCAAGTTTCTTGGTGAAAACCCTAAAGTTTGTGAGAAAGTCCGAACAGCACTTAAAGTGATGAGGCTGGTGCCACCGCTTCAAGGAACGTTCAGAGAAGTTGTTACCGACTTCACCTATGAAGGTTACACAATTCCAAAAGGATGGAAGGTATATTGGTCCTCCAGCAGCACGAATAAAATTGCAGAGCATTTTCCAGCACCAGAAGAGTTTGATCCGAGCAGGTTTGAGAATGGAAAGGCCGCTCCAGCTTACTCAAATATCCCATTCGGAAGCGGTCCTCGAATCTGTCCAGGAAAAGAATATGCTCGACTTCAACTTATGTGTTTCCTCCATCACGTCGTTACAAGATACAAATGGGAAGTGACAAATACAAAGGCCAAAATTGCAGGCGGTTTGAATCCTGTTCCAAAAGAAGGGTTTCACATTCGCATCCAACCTTACAATAAGTTGGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
p450

Protein Analysis

454

Amino Acids

50.92

Weight (kDa)

9.47

Isoelectric Point (pI)

36.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 37 - 319 4.9e-09 Cytochrome P450
p450 PF00067 322 - 427 2.4e-28 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 168
Acc36I ACCTGC 1 cut(s) 1119
AccB1I GGYRCC 1 cut(s) 976
AccBSI CCGCTC 3 cut(s) 345, 557, 1151
AclI AACGTT 1 cut(s) 995
AclWI GGATC 3 cut(s) 800, 813, 1115
AcoI YGGCCR 2 cut(s) 128, 744
AcuI CTGAAG 1 cut(s) 450
AfaI GTAC 1 cut(s) 446
AfiI CCNNNNNNNGG 4 cut(s) 137, 889, 989, 1322
AgsI TTSAA 5 cut(s) 220, 484, 989, 1223, 1306
AhdI GACNNNNNGTC 1 cut(s) 781
AjiI CACGTC 1 cut(s) 1246
AjnI CCWGG 4 cut(s) 112, 412, 661, 1198
AluBI AGCT 3 cut(s) 362, 535, 1157
AluI AGCT 3 cut(s) 362, 535, 1157
Alw26I GTCTC 1 cut(s) 789
AlwI GGATC 3 cut(s) 800, 813, 1115
AoxI GGCC 5 cut(s) 128, 744, 1146, 1284, 1359
ApeKI GCWGC 4 cut(s) 362, 637, 692, 1074
AseI ATTAAT 2 cut(s) 618, 654
Asp700I GAANNNNTTC 3 cut(s) 17, 338, 1326
AspLEI GCGC 1 cut(s) 169
AspS9I GGNCC 3 cut(s) 81, 1065, 1184
AsuC2I CCSGG 1 cut(s) 803
AsuHPI GGTGA 5 cut(s) 152, 248, 367, 932, 1012
AvaII GGWCC 3 cut(s) 81, 1065, 1184
AxyI CCTNAGG 1 cut(s) 631
BalI TGGCCA 1 cut(s) 130
BamHI GGATCC 1 cut(s) 805
BanI GGYRCC 1 cut(s) 976
BanII GRGCYC 1 cut(s) 576
BbsI GAAGAC 2 cut(s) 208, 744
BbvI GCAGC 4 cut(s) 349, 624, 704, 1086
BccI CCATC 3 cut(s) 41, 1044, 1248
BceAI ACGGC 2 cut(s) 233, 731
BciT130I CCWGG 4 cut(s) 114, 414, 663, 1200
BclI TGATCA 1 cut(s) 726
BcnI CCSGG 1 cut(s) 803
BcoDI GTCTC 1 cut(s) 789
BfuAI ACCTGC 1 cut(s) 1119
BisI GCNGC 7 cut(s) 259, 363, 638, 693, 744, 1075, 1149
BlpI GCTNAGC 1 cut(s) 531
BlsI GCNGC 7 cut(s) 260, 364, 639, 694, 745, 1076, 1150
Bme1390I CCNGG 5 cut(s) 114, 414, 663, 803, 1200
Bme18I GGWCC 3 cut(s) 81, 1065, 1184
BmeRI GACNNNNNGTC 1 cut(s) 781
BmgBI CACGTC 1 cut(s) 1246
BmgT120I GGNCC 3 cut(s) 81, 1065, 1184
BmiI GGNNCC 5 cut(s) 575, 667, 763, 807, 978
BmrFI CCNGG 5 cut(s) 114, 414, 663, 803, 1200
BmrI ACTGGG 2 cut(s) 502, 579
BmsI GCATC 2 cut(s) 154, 1347
BmuI ACTGGG 2 cut(s) 502, 579
BoxI GACNNNNGTC 1 cut(s) 322
BpiI GAAGAC 2 cut(s) 208, 744
BpmI CTGGAG 2 cut(s) 1054, 1137
Bpu1102I GCTNAGC 1 cut(s) 531
BpuMI CCSGG 1 cut(s) 803
BsaBI GATNNNNATC 1 cut(s) 620
BsaJI CCNNGG 4 cut(s) 112, 413, 696, 888
BsaWI WCCGGW 2 cut(s) 266, 826
BsaXI ACNNNNNCTCC 4 cut(s) 704, 734, 753, 783
Bsc4I CCNNNNNNNGG 4 cut(s) 137, 889, 989, 1322
Bse1I ACTGG 3 cut(s) 497, 574, 817
Bse21I CCTNAGG 1 cut(s) 631
Bse8I GATNNNNATC 1 cut(s) 620
BseBI CCWGG 4 cut(s) 114, 414, 663, 1200
BseDI CCNNGG 4 cut(s) 112, 413, 696, 888
BseGI GGATG 2 cut(s) 1055, 1338
BseJI GATNNNNATC 1 cut(s) 620
BseLI CCNNNNNNNGG 4 cut(s) 137, 889, 989, 1322
BseMII CTCAG 2 cut(s) 545, 645
BseNI ACTGG 3 cut(s) 497, 574, 817
BseRI GAGGAG 1 cut(s) 337
BseX3I CGGCCG 1 cut(s) 744
BseXI GCAGC 4 cut(s) 349, 624, 704, 1086
Bsh1285I CGRYCG 3 cut(s) 320, 592, 747
BshFI GGCC 5 cut(s) 130, 746, 1148, 1286, 1361
BshNI GGYRCC 1 cut(s) 976
BsiEI CGRYCG 3 cut(s) 320, 592, 747
BsiSI CCGG 4 cut(s) 267, 756, 803, 827
BslI CCNNNNNNNGG 4 cut(s) 137, 889, 989, 1322
BsmAI GTCTC 1 cut(s) 789
BsnI GGCC 5 cut(s) 130, 746, 1148, 1286, 1361
Bsp1286I GDGCHC 1 cut(s) 576
Bsp143I GATC 4 cut(s) 372, 726, 805, 1120
Bsp1720I GCTNAGC 1 cut(s) 531
Bsp19I CCATGG 1 cut(s) 888
BspANI GGCC 5 cut(s) 130, 746, 1148, 1286, 1361
BspCNI CTCAG 2 cut(s) 544, 644
BspLI GGNNCC 5 cut(s) 575, 667, 763, 807, 978
BspMI ACCTGC 1 cut(s) 1119
BspPI GGATC 3 cut(s) 800, 813, 1115
BspT107I GGYRCC 1 cut(s) 976
BsrBI CCGCTC 3 cut(s) 345, 557, 1151
BsrI ACTGG 3 cut(s) 497, 574, 817
BssECI CCNNGG 4 cut(s) 112, 413, 696, 888
BssMI GATC 4 cut(s) 372, 726, 805, 1120
BssT1I CCWWGG 1 cut(s) 888
Bst2UI CCWGG 4 cut(s) 114, 414, 663, 1200
Bst4CI ACNGT 4 cut(s) 303, 511, 593, 819
Bst6I CTCTTC 4 cut(s) 23, 289, 344, 1107
BstC8I GCNNGC 1 cut(s) 1297
BstDEI CTNAG 2 cut(s) 531, 631
BstDSI CCRYGG 1 cut(s) 888
BstF5I GGATG 2 cut(s) 1055, 1338
BstHHI GCGC 1 cut(s) 169
BstKTI GATC 4 cut(s) 375, 729, 808, 1123
BstMAI GTCTC 1 cut(s) 789
BstMBI GATC 4 cut(s) 372, 726, 805, 1120
BstMCI CGRYCG 3 cut(s) 320, 592, 747
BstMWI GCNNNNNNNGC 4 cut(s) 689, 698, 1154, 1292
BstNI CCWGG 4 cut(s) 114, 414, 663, 1200
BstPAI GACNNNNGTC 1 cut(s) 322
BstSCI CCNGG 5 cut(s) 112, 412, 661, 801, 1198
BstV1I GCAGC 4 cut(s) 349, 624, 704, 1086
BstV2I GAAGAC 2 cut(s) 208, 744
BstX2I RGATCY 1 cut(s) 805
BstXI CCANNNNNNTGG 1 cut(s) 1050
BstYI RGATCY 1 cut(s) 805
BstZI CGGCCG 1 cut(s) 744
Bsu36I CCTNAGG 1 cut(s) 631
BsuRI GGCC 5 cut(s) 130, 746, 1148, 1286, 1361
BtgI CCRYGG 1 cut(s) 888
BtgZI GCGATG 1 cut(s) 698
BtrI CACGTC 1 cut(s) 1246
BtsCI GGATG 2 cut(s) 1055, 1338
BtsIMutI CAGTG 1 cut(s) 567
BveI ACCTGC 1 cut(s) 1119
Cac8I GCNNGC 1 cut(s) 1297
CfoI GCGC 1 cut(s) 169
Cfr13I GGNCC 3 cut(s) 81, 1065, 1184
Csp6I GTAC 1 cut(s) 445
CviAII CATG 5 cut(s) 312, 457, 781, 853, 889
CviQI GTAC 1 cut(s) 445
DdeI CTNAG 2 cut(s) 531, 631
DpnI GATC 4 cut(s) 374, 728, 807, 1122
DpnII GATC 4 cut(s) 372, 726, 805, 1120
DriI GACNNNNNGTC 1 cut(s) 781
EaeI YGGCCR 2 cut(s) 128, 744
EagI CGGCCG 1 cut(s) 744
Eam1104I CTCTTC 4 cut(s) 23, 289, 344, 1107
Eam1105I GACNNNNNGTC 1 cut(s) 781
EarI CTCTTC 4 cut(s) 23, 289, 344, 1107
EciI GGCGGA 1 cut(s) 774
EclXI CGGCCG 1 cut(s) 744
Eco130I CCWWGG 1 cut(s) 888
Eco24I GRGCYC 1 cut(s) 576
Eco47I GGWCC 3 cut(s) 81, 1065, 1184
Eco52I CGGCCG 1 cut(s) 744
Eco57I CTGAAG 1 cut(s) 450
Eco81I CCTNAGG 1 cut(s) 631
EcoRII CCWGG 4 cut(s) 112, 412, 661, 1198
EcoT14I CCWWGG 1 cut(s) 888
EcoT38I GRGCYC 1 cut(s) 576
ErhI CCWWGG 1 cut(s) 888
FaeI CATG 5 cut(s) 315, 460, 784, 856, 892
FatI CATG 5 cut(s) 311, 456, 780, 852, 888
FbaI TGATCA 1 cut(s) 726
Fnu4HI GCNGC 7 cut(s) 259, 363, 638, 693, 744, 1075, 1149
FokI GGATG 2 cut(s) 1062, 1325
FriOI GRGCYC 1 cut(s) 576
Fsp4HI GCNGC 7 cut(s) 259, 363, 638, 693, 744, 1075, 1149
FspI TGCGCA 1 cut(s) 168
GlaI GCGC 1 cut(s) 168
GluI GCNGC 7 cut(s) 259, 363, 638, 693, 744, 1075, 1149
GsuI CTGGAG 2 cut(s) 1054, 1137
HaeIII GGCC 5 cut(s) 130, 746, 1148, 1286, 1361
HapII CCGG 4 cut(s) 267, 756, 803, 827
HhaI GCGC 1 cut(s) 169
Hin1II CATG 5 cut(s) 315, 460, 784, 856, 892
Hin6I GCGC 1 cut(s) 167
HinP1I GCGC 1 cut(s) 167
HinfI GANTC 7 cut(s) 103, 389, 461, 547, 561, 1191, 1306
HpaII CCGG 4 cut(s) 267, 756, 803, 827
HphI GGTGA 5 cut(s) 152, 248, 367, 932, 1012
Hpy166II GTNNAC 2 cut(s) 327, 490
Hpy188I TCNGA 7 cut(s) 159, 370, 810, 950, 1001, 1125, 1178
Hpy188III TCNNGA 1 cut(s) 473
Hpy8I GTNNAC 2 cut(s) 327, 490
Hpy99I CGWCG 1 cut(s) 1250
HpyAV CCTTC 9 cut(s) 32, 70, 478, 491, 703, 894, 1022, 1048, 1316
HpyCH4III ACNGT 4 cut(s) 303, 511, 593, 819
HpyCH4IV ACGT 3 cut(s) 644, 995, 1245
HpyCH4V TGCA 4 cut(s) 640, 842, 1091, 1295
HpyF10VI GCNNNNNNNGC 4 cut(s) 689, 698, 1154, 1292
HpyF3I CTNAG 2 cut(s) 531, 631
HpySE526I ACGT 3 cut(s) 644, 995, 1245
Hsp92II CATG 5 cut(s) 315, 460, 784, 856, 892
HspAI GCGC 1 cut(s) 167
Ksp22I TGATCA 1 cut(s) 726
Kzo9I GATC 4 cut(s) 372, 726, 805, 1120
LmnI GCTCC 5 cut(s) 350, 475, 579, 761, 1156
Lsp1109I GCAGC 4 cut(s) 349, 624, 704, 1086
LweI GCATC 2 cut(s) 154, 1347
MaeII ACGT 3 cut(s) 644, 995, 1245
MaeIII GTNAC 7 cut(s) 269, 541, 769, 1009, 1031, 1249, 1270
MalI GATC 4 cut(s) 374, 728, 807, 1122
MbiI CCGCTC 3 cut(s) 345, 557, 1151
MboI GATC 4 cut(s) 372, 726, 805, 1120
MflI RGATCY 1 cut(s) 805
MhlI GDGCHC 1 cut(s) 576
MlsI TGGCCA 1 cut(s) 130
MluCI AATT 4 cut(s) 651, 1038, 1086, 1290
MluNI TGGCCA 1 cut(s) 130
MlyI GAGTC 2 cut(s) 470, 541
MmeI TCCRAC 3 cut(s) 114, 312, 833
Mox20I TGGCCA 1 cut(s) 130
MroXI GAANNNNTTC 3 cut(s) 17, 338, 1326
MscI TGGCCA 1 cut(s) 130
MseI TTAA 6 cut(s) 423, 600, 618, 654, 798, 960
Msp20I TGGCCA 1 cut(s) 130
MspI CCGG 4 cut(s) 267, 756, 803, 827
MspR9I CCNGG 5 cut(s) 114, 414, 663, 803, 1200
MvaI CCWGG 4 cut(s) 114, 414, 663, 1200
MwoI GCNNNNNNNGC 4 cut(s) 689, 698, 1154, 1292
NciI CCSGG 1 cut(s) 803
NcoI CCATGG 1 cut(s) 888
NdeII GATC 4 cut(s) 372, 726, 805, 1120
NlaIII CATG 5 cut(s) 315, 460, 784, 856, 892
NlaIV GGNNCC 5 cut(s) 575, 667, 763, 807, 978
NmeAIII GCCGAG 2 cut(s) 634, 721
NmuCI GTSAC 2 cut(s) 269, 1270
NsbI TGCGCA 1 cut(s) 168
PdmI GAANNNNTTC 3 cut(s) 17, 338, 1326
PfeI GAWTC 5 cut(s) 103, 389, 561, 1191, 1306
PflFI GACNNNGTC 1 cut(s) 550
PfoI TCCNGGA 2 cut(s) 661, 1198
PkrI GCNGC 7 cut(s) 260, 364, 639, 694, 745, 1076, 1150
PleI GAGTC 2 cut(s) 469, 541
PpsI GAGTC 2 cut(s) 469, 541
PshAI GACNNNNGTC 1 cut(s) 322
PshBI ATTAAT 2 cut(s) 618, 654
Psp1406I AACGTT 1 cut(s) 995
Psp6I CCWGG 4 cut(s) 112, 412, 661, 1198
PspGI CCWGG 4 cut(s) 112, 412, 661, 1198
PspN4I GGNNCC 5 cut(s) 575, 667, 763, 807, 978
PspPI GGNCC 3 cut(s) 81, 1065, 1184
PsuI RGATCY 1 cut(s) 805
PsyI GACNNNGTC 1 cut(s) 550
RsaI GTAC 1 cut(s) 446
RsaNI GTAC 1 cut(s) 445
SaqAI TTAA 6 cut(s) 423, 600, 618, 654, 798, 960
SatI GCNGC 7 cut(s) 259, 363, 638, 693, 744, 1075, 1149
Sau3AI GATC 4 cut(s) 372, 726, 805, 1120
Sau96I GGNCC 3 cut(s) 81, 1065, 1184
SchI GAGTC 2 cut(s) 470, 541
ScrFI CCNGG 5 cut(s) 114, 414, 663, 803, 1200
SduI GDGCHC 1 cut(s) 576
SfaNI GCATC 2 cut(s) 154, 1347
SinI GGWCC 3 cut(s) 81, 1065, 1184
Sse9I AATT 4 cut(s) 651, 1038, 1086, 1290
SspI AATATT 1 cut(s) 658
StyD4I CCNGG 5 cut(s) 112, 412, 661, 801, 1198
StyI CCWWGG 1 cut(s) 888
TaaI ACNGT 4 cut(s) 303, 511, 593, 819
TaiI ACGT 3 cut(s) 647, 998, 1248
TaqI TCGA 4 cut(s) 559, 679, 1189, 1216
TasI AATT 4 cut(s) 651, 1038, 1086, 1290
TauI GCSGC 3 cut(s) 261, 746, 1151
TfiI GAWTC 5 cut(s) 103, 389, 561, 1191, 1306
Tru1I TTAA 6 cut(s) 423, 600, 618, 654, 798, 960
Tru9I TTAA 6 cut(s) 423, 600, 618, 654, 798, 960
TscAI CASTG 1 cut(s) 574
TseFI GTSAC 2 cut(s) 269, 1270
TseI GCWGC 4 cut(s) 362, 637, 692, 1074
Tsp45I GTSAC 2 cut(s) 269, 1270
TspDTI ATGAA 7 cut(s) 52, 116, 268, 701, 811, 849, 1041
TspGWI ACGGA 1 cut(s) 34
TspRI CASTG 1 cut(s) 574
Tth111I GACNNNGTC 1 cut(s) 550
VpaK11BI GGWCC 3 cut(s) 81, 1065, 1184
VspI ATTAAT 2 cut(s) 618, 654
XmnI GAANNNNTTC 3 cut(s) 17, 338, 1326
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.