Rroxscaffold_5G00375230

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0000778
Physical Location & Seq
Reverse (-)
56073720 .. 56075952
2233 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00375230.1

Sequence Viewer

Length: 1056 bp
ATGCACGTAGTTAAGGCACCGGGGTTTCTGCGAGCCGAAGCCCTCTTTGGCTACGTGGTAGCTATGGATTCCATGGTTCTCGACCACCTGAAAATGCATTGGGAAGGCAAACAGGTGGTTGAGGCTTATCGCCTCACGCAGATACTCGTTTTGACTCTCTCGGCTAAGTTCTTCATGGGACTCGAAGACCATTGTCGTATTGATAAGCTTGCGGAGTTGATGGACACCATGATGCTGGCGCTTCACACTATTCCTGTGAATATTCCAGGGACTGCATTTCATCAAGCAATGAAAGCAGTGCCGGCTGCGAGGGAGATTATTCAGTCATTTGTTAAGGAGAAGAAGGAAGTTATGGCGTCTACCGGAAACAAAATGCATGATCTTTTGTCTTATATGATTTCTACGCCAGACCCGGTTACCGGAAGGTTCATGCCGGAGAATGAAATTGCTGATAAAATTATGGGGAATGTGGCCGCTTCATTCAACTCTCCAGCTATGACTACTACTTTTATCGTGAAGTTCCTGGGCGAAAGACCTGACATTTATGACAAAGTTCGAATTGAACAACTGGAGATTTTGAAGTCCAAAAAATCTGGCGAGGCACTCAATTGGGATGATATGCAAAAAATGAAGTATTCGTGGAACGTAGCCCTGGAGACGATGAGGCTCATACCACCTCTTCAGGGAACATTTAGAGAGGCTGCAACAGACTTCACTTACCAAGGTTATACAATTCCTAAAGGATGGAAGGTCTACTGGACAGTGAGCACCACAACCATGAACCCTGAATATTTTCCAGCGCCAGAAGAGTTTGATCCTACAAGGTTCGAGAAAGCAAGTCTTCCGCCTTATACAAACATCCCATTCGGGAGCGGTCCTCGAATTTGCCCCGGAAAGGACTACGCTCGTCTGCAAATTCTTAGTTTCATGCACCATCTTGTTATAAGATTCAAATGGGAACTTGTGAACCCTAATTGCAAGATCACCGGTGGAATGAATCCCTTTCCAGTTGATGGGCTGAAGGTTCGCCTCCAAGCTCAACCCAGTCAAGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
p450

Protein Analysis

351

Amino Acids

39.85

Weight (kDa)

8.26

Isoelectric Point (pI)

41.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 38 - 323 3.9e-50 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 944
AccB1I GGYRCC 1 cut(s) 16
AccB7I CCANNNNNTGG 1 cut(s) 1013
AccBSI CCGCTC 1 cut(s) 873
AccI GTMKAC 2 cut(s) 359, 753
AciI CCGC 4 cut(s) 212, 474, 845, 873
AclWI GGATC 1 cut(s) 809
AcoI YGGCCR 1 cut(s) 471
AcsI RAATTY 2 cut(s) 882, 915
AcuI CTGAAG 2 cut(s) 665, 1040
AcyI GRCGYC 1 cut(s) 356
AfiI CCNNNNNNNGG 4 cut(s) 419, 683, 895, 1013
AgeI ACCGGT 1 cut(s) 986
AgsI TTSAA 4 cut(s) 484, 563, 580, 952
AjnI CCWGG 3 cut(s) 265, 522, 651
AjuI GAANNNNNNNTTGG 2 cut(s) 30, 62
AluBI AGCT 5 cut(s) 62, 208, 494, 1037, 1052
AluI AGCT 5 cut(s) 62, 208, 494, 1037, 1052
Alw21I GWGCWC 1 cut(s) 770
Alw26I GTCTC 1 cut(s) 650
AlwI GGATC 1 cut(s) 809
AlwNI CAGNNNCTG 1 cut(s) 272
AoxI GGCC 1 cut(s) 471
ApeKI GCWGC 2 cut(s) 305, 701
ApoI RAATTY 2 cut(s) 882, 915
AsiGI ACCGGT 1 cut(s) 986
Asp700I GAANNNNTTC 1 cut(s) 792
AspLEI GCGC 2 cut(s) 241, 802
AspS9I GGNCC 1 cut(s) 875
AsuC2I CCSGG 3 cut(s) 21, 413, 891
AsuHPI GGTGA 1 cut(s) 976
AsuII TTCGAA 1 cut(s) 556
AvaII GGWCC 1 cut(s) 875
BanI GGYRCC 1 cut(s) 16
BbsI GAAGAC 2 cut(s) 192, 833
Bbv12I GWGCWC 1 cut(s) 770
BbvI GCAGC 2 cut(s) 292, 688
BccI CCATC 4 cut(s) 214, 738, 942, 1007
BciT130I CCWGG 3 cut(s) 267, 524, 653
BcnI CCSGG 3 cut(s) 21, 413, 891
BcoDI GTCTC 1 cut(s) 650
BfoI RGCGCY 2 cut(s) 242, 803
BisI GCNGC 3 cut(s) 306, 474, 702
BlsI GCNGC 3 cut(s) 307, 475, 703
Bme1390I CCNGG 6 cut(s) 21, 267, 413, 524, 653, 891
Bme18I GGWCC 1 cut(s) 875
BmgT120I GGNCC 1 cut(s) 875
BmiI GGNNCC 1 cut(s) 18
BmrFI CCNGG 6 cut(s) 21, 267, 413, 524, 653, 891
BmrI ACTGGG 1 cut(s) 1038
BmsI GCATC 1 cut(s) 222
BmuI ACTGGG 1 cut(s) 1038
BoxI GACNNNNGTC 1 cut(s) 192
BpiI GAAGAC 2 cut(s) 192, 833
BplI GAGNNNNNCTC 2 cut(s) 862, 894
BpmI CTGGAG 3 cut(s) 474, 590, 674
Bpu14I TTCGAA 1 cut(s) 556
BpuMI CCSGG 3 cut(s) 21, 413, 891
BsaAI YACGTR 2 cut(s) 7, 55
BsaHI GRCGYC 1 cut(s) 356
BsaJI CCNNGG 7 cut(s) 20, 72, 266, 523, 651, 721, 889
BsaWI WCCGGW 3 cut(s) 362, 419, 986
Bsc4I CCNNNNNNNGG 4 cut(s) 419, 683, 895, 1013
Bse118I RCCGGY 2 cut(s) 301, 986
Bse1I ACTGG 4 cut(s) 573, 761, 1007, 1044
Bse3DI GCAATG 1 cut(s) 294
BseBI CCWGG 3 cut(s) 267, 524, 653
BseDI CCNNGG 7 cut(s) 20, 72, 266, 523, 651, 721, 889
BseGI GGATG 3 cut(s) 619, 749, 858
BseLI CCNNNNNNNGG 4 cut(s) 419, 683, 895, 1013
BseMI GCAATG 1 cut(s) 294
BseNI ACTGG 4 cut(s) 573, 761, 1007, 1044
BseXI GCAGC 2 cut(s) 292, 688
BshFI GGCC 1 cut(s) 473
BshNI GGYRCC 1 cut(s) 16
BshTI ACCGGT 1 cut(s) 986
BsiHKAI GWGCWC 1 cut(s) 770
BsiSI CCGG 8 cut(s) 20, 302, 363, 413, 420, 434, 891, 987
BslFI GGGAC 2 cut(s) 192, 283
BslI CCNNNNNNNGG 4 cut(s) 419, 683, 895, 1013
BsmAI GTCTC 1 cut(s) 650
BsmBI CGTCTC 1 cut(s) 650
BsmFI GGGAC 2 cut(s) 192, 283
BsnI GGCC 1 cut(s) 473
Bsp119I TTCGAA 1 cut(s) 556
Bsp1286I GDGCHC 1 cut(s) 770
Bsp143I GATC 3 cut(s) 379, 814, 981
Bsp19I CCATGG 1 cut(s) 72
BspACI CCGC 4 cut(s) 212, 474, 845, 873
BspANI GGCC 1 cut(s) 473
BspLI GGNNCC 1 cut(s) 18
BspPI GGATC 1 cut(s) 809
BspT104I TTCGAA 1 cut(s) 556
BspT107I GGYRCC 1 cut(s) 16
BsrBI CCGCTC 1 cut(s) 873
BsrDI GCAATG 1 cut(s) 294
BsrFI RCCGGY 2 cut(s) 301, 986
BsrI ACTGG 4 cut(s) 573, 761, 1007, 1044
BssAI RCCGGY 2 cut(s) 301, 986
BssECI CCNNGG 7 cut(s) 20, 72, 266, 523, 651, 721, 889
BssMI GATC 3 cut(s) 379, 814, 981
BssNI GRCGYC 1 cut(s) 356
BssT1I CCWWGG 2 cut(s) 72, 721
Bst2UI CCWGG 3 cut(s) 267, 524, 653
Bst4CI ACNGT 1 cut(s) 763
Bst6I CTCTTC 2 cut(s) 684, 801
BstACI GRCGYC 1 cut(s) 356
BstBAI YACGTR 2 cut(s) 7, 55
BstBI TTCGAA 1 cut(s) 556
BstC8I GCNNGC 4 cut(s) 33, 210, 237, 303
BstDEI CTNAG 2 cut(s) 165, 920
BstDSI CCRYGG 1 cut(s) 72
BstEII GGTNACC 1 cut(s) 415
BstF5I GGATG 3 cut(s) 619, 749, 858
BstH2I RGCGCY 2 cut(s) 242, 803
BstHHI GCGC 2 cut(s) 241, 802
BstKTI GATC 3 cut(s) 382, 817, 984
BstMAI GTCTC 1 cut(s) 650
BstMBI GATC 3 cut(s) 379, 814, 981
BstMWI GCNNNNNNNGC 2 cut(s) 293, 302
BstNI CCWGG 3 cut(s) 267, 524, 653
BstPAI GACNNNNGTC 1 cut(s) 192
BstPI GGTNACC 1 cut(s) 415
BstSCI CCNGG 6 cut(s) 19, 265, 411, 522, 651, 889
BstV1I GCAGC 2 cut(s) 292, 688
BstV2I GAAGAC 2 cut(s) 192, 833
BstXI CCANNNNNNTGG 1 cut(s) 235
BsuRI GGCC 1 cut(s) 473
BtgI CCRYGG 1 cut(s) 72
BtsCI GGATG 3 cut(s) 619, 749, 858
BtsI GCAGTG 1 cut(s) 303
BtsIMutI CAGTG 2 cut(s) 303, 768
Cac8I GCNNGC 4 cut(s) 33, 210, 237, 303
CaiI CAGNNNCTG 1 cut(s) 272
CfoI GCGC 2 cut(s) 241, 802
Cfr10I RCCGGY 2 cut(s) 301, 986
Cfr13I GGNCC 1 cut(s) 875
CseI GACGC 1 cut(s) 345
CspAI ACCGGT 1 cut(s) 986
CviAII CATG 7 cut(s) 73, 175, 229, 377, 430, 778, 928
DdeI CTNAG 2 cut(s) 165, 920
DpnI GATC 3 cut(s) 381, 816, 983
DpnII GATC 3 cut(s) 379, 814, 981
EaeI YGGCCR 1 cut(s) 471
Eam1104I CTCTTC 2 cut(s) 684, 801
EarI CTCTTC 2 cut(s) 684, 801
EciI GGCGGA 1 cut(s) 834
Eco130I CCWWGG 2 cut(s) 72, 721
Eco47I GGWCC 1 cut(s) 875
Eco57I CTGAAG 2 cut(s) 665, 1040
Eco91I GGTNACC 1 cut(s) 415
EcoO65I GGTNACC 1 cut(s) 415
EcoRII CCWGG 3 cut(s) 265, 522, 651
EcoT14I CCWWGG 2 cut(s) 72, 721
EcoT22I ATGCAT 2 cut(s) 99, 378
ErhI CCWWGG 2 cut(s) 72, 721
Esp3I CGTCTC 1 cut(s) 650
FaeI CATG 7 cut(s) 76, 178, 232, 380, 433, 781, 931
FalI AAGNNNNNCTT 2 cut(s) 825, 857
FaqI GGGAC 2 cut(s) 192, 283
FatI CATG 7 cut(s) 72, 174, 228, 376, 429, 777, 927
FblI GTMKAC 2 cut(s) 359, 753
Fnu4HI GCNGC 3 cut(s) 306, 474, 702
FokI GGATG 3 cut(s) 626, 756, 845
Fsp4HI GCNGC 3 cut(s) 306, 474, 702
GlaI GCGC 2 cut(s) 240, 801
GluI GCNGC 3 cut(s) 306, 474, 702
GsuI CTGGAG 3 cut(s) 474, 590, 674
HaeII RGCGCY 2 cut(s) 242, 803
HaeIII GGCC 1 cut(s) 473
HapII CCGG 8 cut(s) 20, 302, 363, 413, 420, 434, 891, 987
HgaI GACGC 1 cut(s) 345
HhaI GCGC 2 cut(s) 241, 802
Hin1I GRCGYC 1 cut(s) 356
Hin1II CATG 7 cut(s) 76, 178, 232, 380, 433, 781, 931
Hin6I GCGC 2 cut(s) 239, 800
HinP1I GCGC 2 cut(s) 239, 800
HindIII AAGCTT 2 cut(s) 206, 1050
HinfI GANTC 5 cut(s) 68, 154, 180, 948, 997
HpaII CCGG 8 cut(s) 20, 302, 363, 413, 420, 434, 891, 987
HphI GGTGA 1 cut(s) 976
Hpy166II GTNNAC 3 cut(s) 360, 754, 967
Hpy188III TCNNGA 4 cut(s) 80, 514, 829, 868
Hpy8I GTNNAC 3 cut(s) 360, 754, 967
HpyAV CCTTC 5 cut(s) 98, 337, 417, 742, 1015
HpyCH4III ACNGT 1 cut(s) 763
HpyCH4IV ACGT 3 cut(s) 6, 54, 645
HpyCH4V TGCA 9 cut(s) 4, 97, 275, 376, 622, 704, 913, 931, 978
HpyF10VI GCNNNNNNNGC 2 cut(s) 293, 302
HpyF3I CTNAG 2 cut(s) 165, 920
HpySE526I ACGT 3 cut(s) 6, 54, 645
Hsp92I GRCGYC 1 cut(s) 356
Hsp92II CATG 7 cut(s) 76, 178, 232, 380, 433, 781, 931
HspAI GCGC 2 cut(s) 239, 800
KroI GCCGGC 1 cut(s) 301
KroNI GCCGGC 1 cut(s) 303
Kzo9I GATC 3 cut(s) 379, 814, 981
LmnI GCTCC 1 cut(s) 870
Lsp1109I GCAGC 2 cut(s) 292, 688
LweI GCATC 1 cut(s) 222
MaeII ACGT 3 cut(s) 6, 54, 645
MaeIII GTNAC 1 cut(s) 415
MalI GATC 3 cut(s) 381, 816, 983
MbiI CCGCTC 1 cut(s) 873
MboI GATC 3 cut(s) 379, 814, 981
MboII GAAGA 6 cut(s) 163, 197, 352, 671, 818, 833
MfeI CAATTG 1 cut(s) 607
MhlI GDGCHC 1 cut(s) 770
MluCI AATT 8 cut(s) 444, 456, 558, 607, 732, 882, 915, 973
MlyI GAGTC 2 cut(s) 148, 174
Mph1103I ATGCAT 2 cut(s) 99, 378
MroNI GCCGGC 1 cut(s) 301
MroXI GAANNNNTTC 1 cut(s) 792
MseI TTAA 3 cut(s) 12, 333, 1054
MslI CAYNNNNRTG 2 cut(s) 230, 776
MspI CCGG 8 cut(s) 20, 302, 363, 413, 420, 434, 891, 987
MspR9I CCNGG 6 cut(s) 21, 267, 413, 524, 653, 891
MunI CAATTG 1 cut(s) 607
MvaI CCWGG 3 cut(s) 267, 524, 653
MwoI GCNNNNNNNGC 2 cut(s) 293, 302
NaeI GCCGGC 1 cut(s) 303
NciI CCSGG 3 cut(s) 21, 413, 891
NcoI CCATGG 1 cut(s) 72
NdeII GATC 3 cut(s) 379, 814, 981
NgoMIV GCCGGC 1 cut(s) 301
NlaIII CATG 7 cut(s) 76, 178, 232, 380, 433, 781, 931
NlaIV GGNNCC 1 cut(s) 18
NmeAIII GCCGAG 1 cut(s) 140
NsiI ATGCAT 2 cut(s) 99, 378
NspV TTCGAA 1 cut(s) 556
PdiI GCCGGC 1 cut(s) 303
PdmI GAANNNNTTC 1 cut(s) 792
PfeI GAWTC 3 cut(s) 68, 948, 997
PflMI CCANNNNNTGG 1 cut(s) 1013
PinAI ACCGGT 1 cut(s) 986
PkrI GCNGC 3 cut(s) 307, 475, 703
PleI GAGTC 2 cut(s) 148, 174
PpsI GAGTC 2 cut(s) 148, 174
Ppu21I YACGTR 2 cut(s) 7, 55
PshAI GACNNNNGTC 1 cut(s) 192
PsiI TTATAA 1 cut(s) 944
Psp6I CCWGG 3 cut(s) 265, 522, 651
PspEI GGTNACC 1 cut(s) 415
PspGI CCWGG 3 cut(s) 265, 522, 651
PspN4I GGNNCC 1 cut(s) 18
PspPI GGNCC 1 cut(s) 875
PstNI CAGNNNCTG 1 cut(s) 272
RseI CAYNNNNRTG 2 cut(s) 230, 776
SaqAI TTAA 3 cut(s) 12, 333, 1054
SatI GCNGC 3 cut(s) 306, 474, 702
Sau3AI GATC 3 cut(s) 379, 814, 981
Sau96I GGNCC 1 cut(s) 875
SchI GAGTC 2 cut(s) 148, 174
ScrFI CCNGG 6 cut(s) 21, 267, 413, 524, 653, 891
SduI GDGCHC 1 cut(s) 770
SfaNI GCATC 1 cut(s) 222
SfuI TTCGAA 1 cut(s) 556
SgrAI CRCCGGYG 1 cut(s) 986
SinI GGWCC 1 cut(s) 875
SmiMI CAYNNNNRTG 2 cut(s) 230, 776
Sse9I AATT 8 cut(s) 444, 456, 558, 607, 732, 882, 915, 973
SsiI CCGC 4 cut(s) 212, 474, 845, 873
SspI AATATT 2 cut(s) 262, 791
StyD4I CCNGG 6 cut(s) 19, 265, 411, 522, 651, 889
StyI CCWWGG 2 cut(s) 72, 721
TaaI ACNGT 1 cut(s) 763
TaiI ACGT 3 cut(s) 9, 57, 648
TaqI TCGA 5 cut(s) 81, 183, 556, 828, 880
TasI AATT 8 cut(s) 444, 456, 558, 607, 732, 882, 915, 973
TauI GCSGC 1 cut(s) 476
TfiI GAWTC 3 cut(s) 68, 948, 997
Tru1I TTAA 3 cut(s) 12, 333, 1054
Tru9I TTAA 3 cut(s) 12, 333, 1054
TscAI CASTG 2 cut(s) 303, 768
TseI GCWGC 2 cut(s) 305, 701
TspRI CASTG 2 cut(s) 303, 768
Van91I CCANNNNNTGG 1 cut(s) 1013
VpaK11BI GGWCC 1 cut(s) 875
XapI RAATTY 2 cut(s) 882, 915
XmiI GTMKAC 2 cut(s) 359, 753
XmnI GAANNNNTTC 1 cut(s) 792
Zsp2I ATGCAT 2 cut(s) 99, 378
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.