Rw4G028250

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0000778
Physical Location & Seq
Reverse (-)
54918270 .. 54920323
2054 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G028250.1

Sequence Viewer

Length: 1458 bp
ATGGATTACAACCTTTACATGTTCTTGTCTACACTACTTGCCATCTTCACTACTACTACTCTTGCATTCCTTTTTTGTGCTTATAAATCAAAATGCCATGAAAAATTCATTAATCTTCCCCCAGGCAGTTTTGGCTGGCCAATTATCGGCGAAACCTTTGCTTTCCTACACAATGACCATGAAAAATTTGTAGGGAATAGAATGAAGAAATACTCCTCCAAAATCTTCAAAACCAAGATACTAGGTGAGCCAACCGTGGTGTTATGTGGCACTGCCGGTCACAAATTTGTGGCTTCCAATGAAGAAAAGCTCTTTGTAGCATGGAGACCTCACTCAATGCAAAAGCTCTTCCGCTCTTCTTACCAAAAAGCTGCTTCCACCGTCATACCAAGACAATTCGAAAAGCACGTAGCTCGGGCACCGGGGTTTCTGAGAGCCGAAGCCCTGGTTGGCTACGTGGGAAGGATGGATTCCATGGTTCTCGACCACCTGAAAACACACTGGGATGGCAAACAGGTGGTCGAGGCTCATCGCCTCACGCAACTACTCGTTTTGACTCTCTCGGCTAAGTTCTTCATGGGACTCAAAGACCATTGTCGTATTGATAAGCTTGCAGAGTTGATGGACACCATGATGCTGGCGCTTCACACTGTTCCAGTGAATATTCCGGGAACTGCATTTTATCAAGCAATGAAAGCAGCGCAGGCTGTGAGGGAGACTATTCAGTCGTTTATTAAGGAGAAGAAGGAAGTAATGGCGTCTACCGGAAACAAAATGCATGATCTTTTGTCTTATTTGATTTCTACGGCCAACCCGGTTACCGGAAGGTTCATGCCGGAGAATGAAATTGCTGATAAAATTATGGGGACTGTGGCCGCTTCGTTCAACTCTCCAGCTATGACTACTACTTTTATCGTGAAGTTCCTGGGCGAAAGACCTGACATTTATGACAAAGTTCGAATTGAACAACTGGAGATTTTGAAGTCCAAAAAATCTGGCGAGGCACTCAATTGGGATGATATGCAAAAAATGAAGTATTCGTGGAACGTAGCACTGGAGGCGATGAGGCTCATACCACCTCTTCAGGGAACATTTAGAGAGGCTGCAACAGACTTCACTTACGAAGGTTTTACAATTCCTAAAGGATGGAAGGTTTATTGGACAGTGAGCACCACAAACATGAACCCTGAATATTTTCCAGCGCCAGAAGAGTTTGATCCTACAAGGTTCGAGAAAGCAACTCCTCCGCCTTACACAAACATCCCATTTGGGAGCGGTCCTCGAATCTGCCCCGGAATGGACTACGCTCGTCTGCAAATTCTTAGTTTTATGCACCATCTTGTTATAAGATTCAAATGGGAACTTGTGAATCCTAATTGCAAGATCTCAGGTGGTATGAATCCCTTTCCAGTTGATGGGCTGAAGGTTCGCCTCCAAGCTCAACCCATTCAAGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
p450

Protein Analysis

485

Amino Acids

55.14

Weight (kDa)

9.26

Isoelectric Point (pI)

32.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 40 - 457 1.3e-55 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 84, 1346
AasI GACNNNNNNGTC 1 cut(s) 724
AccB1I GGYRCC 1 cut(s) 418
AccB7I CCANNNNNTGG 1 cut(s) 1415
AccBSI CCGCTC 2 cut(s) 354, 1275
AccI GTMKAC 2 cut(s) 29, 761
AciI CCGC 4 cut(s) 352, 876, 1247, 1275
AclWI GGATC 1 cut(s) 1211
AcoI YGGCCR 3 cut(s) 137, 807, 873
AcsI RAATTY 4 cut(s) 104, 185, 284, 1317
AcuI CTGAAG 2 cut(s) 1067, 1442
AcyI GRCGYC 1 cut(s) 758
AfiI CCNNNNNNNGG 5 cut(s) 146, 821, 1085, 1297, 1415
AflIII ACRYGT 1 cut(s) 18
AgsI TTSAA 6 cut(s) 229, 886, 965, 982, 1354, 1451
AjnI CCWGG 3 cut(s) 121, 444, 924
AjuI GAANNNNNNNTTGG 2 cut(s) 432, 464
AluBI AGCT 8 cut(s) 310, 346, 371, 413, 610, 896, 1439, 1454
AluI AGCT 8 cut(s) 310, 346, 371, 413, 610, 896, 1439, 1454
Alw21I GWGCWC 1 cut(s) 1172
Alw26I GTCTC 2 cut(s) 319, 710
AlwI GGATC 1 cut(s) 1211
Ama87I CYCGRG 1 cut(s) 414
AoxI GGCC 3 cut(s) 137, 807, 873
ApeKI GCWGC 3 cut(s) 371, 698, 1103
ApoI RAATTY 4 cut(s) 104, 185, 284, 1317
ArsI GACNNNNNNTTYG 2 cut(s) 504, 536
AseI ATTAAT 1 cut(s) 111
Asp700I GAANNNNTTC 1 cut(s) 1194
AspLEI GCGC 3 cut(s) 643, 703, 1204
AspS9I GGNCC 1 cut(s) 1277
AsuC2I CCSGG 4 cut(s) 423, 669, 815, 1293
AsuHPI GGTGA 1 cut(s) 257
AsuII TTCGAA 2 cut(s) 399, 958
AvaI CYCGRG 1 cut(s) 414
AvaII GGWCC 1 cut(s) 1277
BaeGI GKGCMC 1 cut(s) 421
BalI TGGCCA 1 cut(s) 139
BanI GGYRCC 1 cut(s) 418
Bbv12I GWGCWC 1 cut(s) 1172
BbvI GCAGC 3 cut(s) 358, 710, 1090
BccI CCATC 7 cut(s) 50, 460, 500, 616, 1140, 1344, 1409
BceAI ACGGC 1 cut(s) 822
BcgI CGANNNNNNTGC 4 cut(s) 140, 174, 395, 429
BciT130I CCWGG 3 cut(s) 123, 446, 926
BcnI CCSGG 4 cut(s) 423, 669, 815, 1293
BcoDI GTCTC 2 cut(s) 319, 710
BfaI CTAG 1 cut(s) 242
BfoI RGCGCY 2 cut(s) 644, 1205
BglII AGATCT 1 cut(s) 1383
BisI GCNGC 4 cut(s) 372, 699, 876, 1104
BlsI GCNGC 4 cut(s) 373, 700, 877, 1105
Bme1390I CCNGG 7 cut(s) 123, 423, 446, 669, 815, 926, 1293
Bme18I GGWCC 1 cut(s) 1277
BmeT110I CYCGRG 1 cut(s) 414
BmgT120I GGNCC 1 cut(s) 1277
BmiI GGNNCC 1 cut(s) 420
BmrFI CCNGG 7 cut(s) 123, 423, 446, 669, 815, 926, 1293
BmrI ACTGGG 1 cut(s) 511
BmsI GCATC 1 cut(s) 624
BmuI ACTGGG 1 cut(s) 511
BoxI GACNNNNGTC 1 cut(s) 594
BplI GAGNNNNNCTC 2 cut(s) 1264, 1296
BpmI CTGGAG 3 cut(s) 876, 992, 1076
Bpu14I TTCGAA 2 cut(s) 399, 958
BpuMI CCSGG 4 cut(s) 423, 669, 815, 1293
BsaAI YACGTR 2 cut(s) 409, 457
BsaHI GRCGYC 1 cut(s) 758
BsaI GGTCTC 1 cut(s) 319
BsaJI CCNNGG 7 cut(s) 121, 255, 422, 444, 474, 925, 1291
BsaWI WCCGGW 2 cut(s) 764, 821
Bsc4I CCNNNNNNNGG 5 cut(s) 146, 821, 1085, 1297, 1415
Bse118I RCCGGY 1 cut(s) 275
Bse1I ACTGG 5 cut(s) 506, 656, 975, 1059, 1409
Bse3DI GCAATG 1 cut(s) 696
BseBI CCWGG 3 cut(s) 123, 446, 926
BseDI CCNNGG 7 cut(s) 121, 255, 422, 444, 474, 925, 1291
BseGI GGATG 5 cut(s) 471, 511, 1021, 1151, 1260
BseLI CCNNNNNNNGG 5 cut(s) 146, 821, 1085, 1297, 1415
BseMI GCAATG 1 cut(s) 696
BseMII CTCAG 2 cut(s) 422, 1401
BseNI ACTGG 5 cut(s) 506, 656, 975, 1059, 1409
BseRI GAGGAG 2 cut(s) 205, 1233
BseSI GKGCMC 1 cut(s) 421
BseXI GCAGC 3 cut(s) 358, 710, 1090
BshFI GGCC 3 cut(s) 139, 809, 875
BshNI GGYRCC 1 cut(s) 418
BsiHKAI GWGCWC 1 cut(s) 1172
BsiHKCI CYCGRG 1 cut(s) 414
BsiSI CCGG 8 cut(s) 276, 422, 668, 765, 815, 822, 836, 1293
BslFI GGGAC 2 cut(s) 594, 880
BslI CCNNNNNNNGG 5 cut(s) 146, 821, 1085, 1297, 1415
BsmAI GTCTC 2 cut(s) 319, 710
BsmFI GGGAC 2 cut(s) 594, 880
BsmI GAATGC 1 cut(s) 65
BsnI GGCC 3 cut(s) 139, 809, 875
Bso31I GGTCTC 1 cut(s) 319
BsoBI CYCGRG 1 cut(s) 414
Bsp119I TTCGAA 2 cut(s) 399, 958
Bsp1286I GDGCHC 2 cut(s) 421, 1172
Bsp143I GATC 3 cut(s) 781, 1216, 1383
Bsp19I CCATGG 1 cut(s) 474
BspACI CCGC 4 cut(s) 352, 876, 1247, 1275
BspANI GGCC 3 cut(s) 139, 809, 875
BspCNI CTCAG 2 cut(s) 423, 1400
BspLI GGNNCC 1 cut(s) 420
BspPI GGATC 1 cut(s) 1211
BspQI GCTCTTC 2 cut(s) 353, 361
BspT104I TTCGAA 2 cut(s) 399, 958
BspT107I GGYRCC 1 cut(s) 418
BspTNI GGTCTC 1 cut(s) 319
BsrBI CCGCTC 2 cut(s) 354, 1275
BsrDI GCAATG 1 cut(s) 696
BsrFI RCCGGY 1 cut(s) 275
BsrI ACTGG 5 cut(s) 506, 656, 975, 1059, 1409
BssAI RCCGGY 1 cut(s) 275
BssECI CCNNGG 7 cut(s) 121, 255, 422, 444, 474, 925, 1291
BssMI GATC 3 cut(s) 781, 1216, 1383
BssNI GRCGYC 1 cut(s) 758
BssT1I CCWWGG 1 cut(s) 474
Bst2UI CCWGG 3 cut(s) 123, 446, 926
Bst4CI ACNGT 5 cut(s) 256, 382, 652, 871, 1165
Bst6I CTCTTC 4 cut(s) 353, 361, 1086, 1203
BstACI GRCGYC 1 cut(s) 758
BstBAI YACGTR 2 cut(s) 409, 457
BstBI TTCGAA 2 cut(s) 399, 958
BstC8I GCNNGC 4 cut(s) 137, 612, 639, 705
BstDEI CTNAG 4 cut(s) 431, 567, 1322, 1387
BstDSI CCRYGG 2 cut(s) 255, 474
BstEII GGTNACC 1 cut(s) 817
BstF5I GGATG 5 cut(s) 471, 511, 1021, 1151, 1260
BstH2I RGCGCY 2 cut(s) 644, 1205
BstHHI GCGC 3 cut(s) 643, 703, 1204
BstKTI GATC 3 cut(s) 784, 1219, 1386
BstMAI GTCTC 2 cut(s) 319, 710
BstMBI GATC 3 cut(s) 781, 1216, 1383
BstMWI GCNNNNNNNGC 4 cut(s) 132, 695, 704, 1058
BstNI CCWGG 3 cut(s) 123, 446, 926
BstNSI RCATGY 1 cut(s) 22
BstPAI GACNNNNGTC 1 cut(s) 594
BstPI GGTNACC 1 cut(s) 817
BstSCI CCNGG 7 cut(s) 121, 421, 444, 667, 813, 924, 1291
BstSLI GKGCMC 1 cut(s) 421
BstV1I GCAGC 3 cut(s) 358, 710, 1090
BstX2I RGATCY 1 cut(s) 1383
BstXI CCANNNNNNTGG 1 cut(s) 637
BstYI RGATCY 1 cut(s) 1383
BsuRI GGCC 3 cut(s) 139, 809, 875
BtgI CCRYGG 2 cut(s) 255, 474
BtgZI GCGATG 2 cut(s) 515, 1076
BtsCI GGATG 5 cut(s) 471, 511, 1021, 1151, 1260
BtsI GCAGTG 1 cut(s) 270
BtsIMutI CAGTG 6 cut(s) 270, 499, 648, 663, 1052, 1170
Cac8I GCNNGC 4 cut(s) 137, 612, 639, 705
CfoI GCGC 3 cut(s) 643, 703, 1204
Cfr10I RCCGGY 1 cut(s) 275
Cfr13I GGNCC 1 cut(s) 1277
CseI GACGC 1 cut(s) 747
DdeI CTNAG 4 cut(s) 431, 567, 1322, 1387
DpnI GATC 3 cut(s) 783, 1218, 1385
DpnII GATC 3 cut(s) 781, 1216, 1383
DrdI GACNNNNNNGTC 1 cut(s) 724
DseDI GACNNNNNNGTC 1 cut(s) 724
EaeI YGGCCR 3 cut(s) 137, 807, 873
Eam1104I CTCTTC 4 cut(s) 353, 361, 1086, 1203
EarI CTCTTC 4 cut(s) 353, 361, 1086, 1203
EciI GGCGGA 1 cut(s) 1236
Eco130I CCWWGG 1 cut(s) 474
Eco31I GGTCTC 1 cut(s) 319
Eco47I GGWCC 1 cut(s) 1277
Eco57I CTGAAG 2 cut(s) 1067, 1442
Eco88I CYCGRG 1 cut(s) 414
Eco91I GGTNACC 1 cut(s) 817
EcoO65I GGTNACC 1 cut(s) 817
EcoRII CCWGG 3 cut(s) 121, 444, 924
EcoT14I CCWWGG 1 cut(s) 474
EcoT22I ATGCAT 1 cut(s) 780
ErhI CCWWGG 1 cut(s) 474
FaqI GGGAC 2 cut(s) 594, 880
FblI GTMKAC 2 cut(s) 29, 761
Fnu4HI GCNGC 4 cut(s) 372, 699, 876, 1104
FokI GGATG 5 cut(s) 478, 518, 1028, 1158, 1247
Fsp4HI GCNGC 4 cut(s) 372, 699, 876, 1104
FspBI CTAG 1 cut(s) 242
GlaI GCGC 3 cut(s) 642, 702, 1203
GluI GCNGC 4 cut(s) 372, 699, 876, 1104
GsuI CTGGAG 3 cut(s) 876, 992, 1076
HaeII RGCGCY 2 cut(s) 644, 1205
HaeIII GGCC 3 cut(s) 139, 809, 875
HapII CCGG 8 cut(s) 276, 422, 668, 765, 815, 822, 836, 1293
HgaI GACGC 1 cut(s) 747
HhaI GCGC 3 cut(s) 643, 703, 1204
Hin1I GRCGYC 1 cut(s) 758
Hin6I GCGC 3 cut(s) 641, 701, 1202
HinP1I GCGC 3 cut(s) 641, 701, 1202
HindIII AAGCTT 2 cut(s) 608, 1452
HinfI GANTC 7 cut(s) 470, 556, 582, 1284, 1350, 1369, 1399
HpaII CCGG 8 cut(s) 276, 422, 668, 765, 815, 822, 836, 1293
HphI GGTGA 1 cut(s) 257
Hpy166II GTNNAC 2 cut(s) 30, 762
Hpy188I TCNGA 1 cut(s) 432
Hpy188III TCNNGA 3 cut(s) 482, 916, 1231
Hpy8I GTNNAC 2 cut(s) 30, 762
HpyAV CCTTC 6 cut(s) 456, 739, 819, 1118, 1144, 1417
HpyCH4III ACNGT 5 cut(s) 256, 382, 652, 871, 1165
HpyCH4IV ACGT 3 cut(s) 408, 456, 1047
HpyF10VI GCNNNNNNNGC 4 cut(s) 132, 695, 704, 1058
HpyF3I CTNAG 4 cut(s) 431, 567, 1322, 1387
HpySE526I ACGT 3 cut(s) 408, 456, 1047
Hsp92I GRCGYC 1 cut(s) 758
HspAI GCGC 3 cut(s) 641, 701, 1202
Kzo9I GATC 3 cut(s) 781, 1216, 1383
LguI GCTCTTC 2 cut(s) 353, 361
LmnI GCTCC 1 cut(s) 1272
Lsp1109I GCAGC 3 cut(s) 358, 710, 1090
LweI GCATC 1 cut(s) 624
MaeI CTAG 1 cut(s) 242
MaeII ACGT 3 cut(s) 408, 456, 1047
MaeIII GTNAC 2 cut(s) 278, 817
MalI GATC 3 cut(s) 783, 1218, 1385
MbiI CCGCTC 2 cut(s) 354, 1275
MboI GATC 3 cut(s) 781, 1216, 1383
MfeI CAATTG 1 cut(s) 1009
MflI RGATCY 1 cut(s) 1383
MhlI GDGCHC 2 cut(s) 421, 1172
MlsI TGGCCA 1 cut(s) 139
MluNI TGGCCA 1 cut(s) 139
MlyI GAGTC 2 cut(s) 550, 576
Mox20I TGGCCA 1 cut(s) 139
Mph1103I ATGCAT 1 cut(s) 780
MroXI GAANNNNTTC 1 cut(s) 1194
MscI TGGCCA 1 cut(s) 139
MseI TTAA 3 cut(s) 111, 735, 1456
MslI CAYNNNNRTG 3 cut(s) 504, 632, 1178
Msp20I TGGCCA 1 cut(s) 139
MspI CCGG 8 cut(s) 276, 422, 668, 765, 815, 822, 836, 1293
MspR9I CCNGG 7 cut(s) 123, 423, 446, 669, 815, 926, 1293
MunI CAATTG 1 cut(s) 1009
Mva1269I GAATGC 1 cut(s) 65
MvaI CCWGG 3 cut(s) 123, 446, 926
MwoI GCNNNNNNNGC 4 cut(s) 132, 695, 704, 1058
NciI CCSGG 4 cut(s) 423, 669, 815, 1293
NcoI CCATGG 1 cut(s) 474
NdeII GATC 3 cut(s) 781, 1216, 1383
NlaIV GGNNCC 1 cut(s) 420
NmeAIII GCCGAG 1 cut(s) 542
NmuCI GTSAC 1 cut(s) 278
NsiI ATGCAT 1 cut(s) 780
NspI RCATGY 1 cut(s) 22
NspV TTCGAA 2 cut(s) 399, 958
PciI ACATGT 1 cut(s) 18
PciSI GCTCTTC 2 cut(s) 353, 361
PcsI WCGNNNNNNNCGW 1 cut(s) 405
PctI GAATGC 1 cut(s) 65
PdmI GAANNNNTTC 1 cut(s) 1194
PfeI GAWTC 5 cut(s) 470, 1284, 1350, 1369, 1399
PflMI CCANNNNNTGG 1 cut(s) 1415
PfoI TCCNGGA 1 cut(s) 667
PkrI GCNGC 4 cut(s) 373, 700, 877, 1105
PleI GAGTC 2 cut(s) 550, 576
PpsI GAGTC 2 cut(s) 550, 576
Ppu21I YACGTR 2 cut(s) 409, 457
PscI ACATGT 1 cut(s) 18
PshAI GACNNNNGTC 1 cut(s) 594
PshBI ATTAAT 1 cut(s) 111
PsiI TTATAA 2 cut(s) 84, 1346
Psp6I CCWGG 3 cut(s) 121, 444, 924
PspEI GGTNACC 1 cut(s) 817
PspGI CCWGG 3 cut(s) 121, 444, 924
PspN4I GGNNCC 1 cut(s) 420
PspPI GGNCC 1 cut(s) 1277
PsuI RGATCY 1 cut(s) 1383
RseI CAYNNNNRTG 3 cut(s) 504, 632, 1178
SapI GCTCTTC 2 cut(s) 353, 361
SaqAI TTAA 3 cut(s) 111, 735, 1456
SatI GCNGC 4 cut(s) 372, 699, 876, 1104
Sau3AI GATC 3 cut(s) 781, 1216, 1383
Sau96I GGNCC 1 cut(s) 1277
SchI GAGTC 2 cut(s) 550, 576
ScrFI CCNGG 7 cut(s) 123, 423, 446, 669, 815, 926, 1293
SduI GDGCHC 2 cut(s) 421, 1172
SfaNI GCATC 1 cut(s) 624
SfuI TTCGAA 2 cut(s) 399, 958
SinI GGWCC 1 cut(s) 1277
SmiMI CAYNNNNRTG 3 cut(s) 504, 632, 1178
SsiI CCGC 4 cut(s) 352, 876, 1247, 1275
SspI AATATT 2 cut(s) 664, 1193
SspMI CTAG 1 cut(s) 242
StyD4I CCNGG 7 cut(s) 121, 421, 444, 667, 813, 924, 1291
StyI CCWWGG 1 cut(s) 474
TaaI ACNGT 5 cut(s) 256, 382, 652, 871, 1165
TaiI ACGT 3 cut(s) 411, 459, 1050
TaqI TCGA 6 cut(s) 399, 483, 522, 958, 1230, 1282
TauI GCSGC 1 cut(s) 878
TfiI GAWTC 5 cut(s) 470, 1284, 1350, 1369, 1399
Tru1I TTAA 3 cut(s) 111, 735, 1456
Tru9I TTAA 3 cut(s) 111, 735, 1456
TscAI CASTG 6 cut(s) 277, 506, 655, 663, 1059, 1170
TseFI GTSAC 1 cut(s) 278
TseI GCWGC 3 cut(s) 371, 698, 1103
Tsp45I GTSAC 1 cut(s) 278
TspRI CASTG 6 cut(s) 277, 506, 655, 663, 1059, 1170
Van91I CCANNNNNTGG 1 cut(s) 1415
VpaK11BI GGWCC 1 cut(s) 1277
VspI ATTAAT 1 cut(s) 111
XapI RAATTY 4 cut(s) 104, 185, 284, 1317
XceI RCATGY 1 cut(s) 22
XmiI GTMKAC 2 cut(s) 29, 761
XmnI GAANNNNTTC 1 cut(s) 1194
XspI CTAG 1 cut(s) 242
Zsp2I ATGCAT 1 cut(s) 780
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.