FvH4_3g24410

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0000778
Physical Location & Seq
Reverse (-)
17482972 .. 17485172
2201 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g24410.t1

Sequence Viewer

Length: 1461 bp
ATGGATTACAACTTTTACATCTTCTTCTCTACACTACTTGCCATATTCTTCACAACTACTATTCTTGCAGTCTTCTTTCGTGCTTATAAATCAAAATCCGATGAAAAATCCACCAACCTTCCCCCAGGCAGTTTCGGGTGGCCGATTATCGGCGAAACCTTTGCCTTCCTATACGATGACCATGAAAAGTTTGTAGGAGAAAGAATGAAGAAATACTCCTCCAAGATCTTCAAAACCAAAATACTAGGGGAGCCAACCGTGGTGTTATGTGGCACTGCCGGTCACAAATTTGTGGCCTCCAATGAAGATAAGCTCTTTGCAGCATGGAGACCTCACTCCATGCAAAGGCTCTTCCGCTCTTCTTACCAAAAAGCTGCTTCCACTGTCATACCGAGAATAAGTGAAATGCACGTATCTCGAGCACCGGGGTTTCTGAGAGCGGAAGCCCTCGTTGGCTACGTGGGGCAGATGGATGCGATGGTTCTCGACCACCTGAAAATGTACTGGGATGGCAAACAGGTGGTTGAGGCTCATCGCCTCTCGCAGCTACTCGTCTTGACTCTCGCGACTAAGTTCTTCATGGGACTCGAAGACCATGGTCGTATTGATAAGCTTGTAGATCTGATGGACACCATGATGCTGGCACTTCACACTATGCCGGTGAATATTCCAGGGACTGTGTTTTATCGAGCAATGAAAGCAACGCAGGCTGCAAGGGAGATATTTCAGTCGTTTATTAAGGAGAAGAGAGCAGCTATTGCGTCTACCGGAAACAAAGTGCATGATGTTTTGTCCTATATGATTTCTACTCCAGATCCGGGTACCGGTAAGTTTATGCCGGATAATGAAATTGCTGATAAAATTATGGGGAATGTGGCAGCTTCGTTCAACTCTCCGGCCATGACTACTACTTTTATCGTCAAGTTCCTCGCCCAGAGACCTGACATTTATGAAAAAGTCCGACTAGAACAACTGGAGATTTTGAAGTCCAAGAAATCTGGGGAGCCACTCAACTGGGAGGACATGCAAAAAATGAAGTATTCGTGGAACGTAGCCCTAGAGGCGATGAGGATCATACCACCTCTTCAGGGAACCTTTAGAGAGGCTGCAAAAGACTTCACTTACCAAGGTTATACAATTCCAAAAGGATGGAAGGTCTACTGGACAGTGAGCACCACAAACATGAACACTGAATATTTTGCAGCGCCAGAAGAGTTCGATCCGACAAGATACGAGAAAACAAGTCTTCCTCCTTACACAAACATCCCATTCGGGAGCGGCCCTCGAATTTGCCCCGGAAAGGACTACGCTCGTCTGCAAATTCTTAGCTTCATGCACCACCTTGTTATAAGATTCAAATGGGAACTTGTAAACCCTAACTGCAAGATCACAGGTGGTATGAATCCCTTTCCAGTTGATGGGCTCAAGGTTCGCCTCCAAGCTCAACCCCTTCCAGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
p450

Protein Analysis

487

Amino Acids

55.33

Weight (kDa)

9.28

Isoelectric Point (pI)

31.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 41 - 458 4e-54 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 87, 1349
Acc65I GGTACC 1 cut(s) 821
AccB1I GGYRCC 1 cut(s) 821
AccB7I CCANNNNNTGG 1 cut(s) 1418
AccBSI CCGCTC 3 cut(s) 357, 440, 1278
AccI GTMKAC 2 cut(s) 764, 1158
AccII CGCG 1 cut(s) 566
AciI CCGC 3 cut(s) 355, 440, 1278
AclWI GGATC 3 cut(s) 809, 1079, 1214
AcoI YGGCCR 2 cut(s) 140, 897
AcsI RAATTY 3 cut(s) 287, 1287, 1320
AcuI CTGAAG 1 cut(s) 1070
AfaI GTAC 2 cut(s) 503, 823
AfiI CCNNNNNNNGG 7 cut(s) 149, 345, 818, 824, 1088, 1300, 1418
AgeI ACCGGT 1 cut(s) 824
AgsI TTSAA 4 cut(s) 232, 889, 985, 1357
AjnI CCWGG 2 cut(s) 124, 670
AjuI GAANNNNNNNTTGG 2 cut(s) 435, 467
AluBI AGCT 9 cut(s) 313, 374, 547, 613, 755, 881, 1329, 1442, 1457
AluI AGCT 9 cut(s) 313, 374, 547, 613, 755, 881, 1329, 1442, 1457
Alw21I GWGCWC 2 cut(s) 424, 1175
Alw26I GTCTC 2 cut(s) 322, 931
AlwI GGATC 3 cut(s) 809, 1079, 1214
AlwNI CAGNNNCTG 1 cut(s) 677
Ama87I CYCGRG 1 cut(s) 417
AoxI GGCC 4 cut(s) 140, 294, 897, 1279
ApeKI GCWGC 8 cut(s) 320, 374, 544, 710, 752, 878, 1106, 1202
ApoI RAATTY 3 cut(s) 287, 1287, 1320
AsiGI ACCGGT 1 cut(s) 824
Asp718I GGTACC 1 cut(s) 821
AspLEI GCGC 1 cut(s) 1207
AspS9I GGNCC 1 cut(s) 1280
AsuC2I CCSGG 3 cut(s) 426, 819, 1296
AsuHPI GGTGA 1 cut(s) 673
AvaI CYCGRG 1 cut(s) 417
BanI GGYRCC 1 cut(s) 821
BanII GRGCYC 1 cut(s) 1425
BbsI GAAGAC 3 cut(s) 64, 597, 1238
Bbv12I GWGCWC 2 cut(s) 424, 1175
BbvI GCAGC 8 cut(s) 332, 361, 556, 697, 764, 890, 1093, 1214
BccI CCATC 6 cut(s) 463, 472, 503, 619, 1143, 1412
BcgI CGANNNNNNTGC 4 cut(s) 143, 177, 398, 432
BciT130I CCWGG 2 cut(s) 126, 672
BcnI CCSGG 3 cut(s) 426, 819, 1296
BcoDI GTCTC 2 cut(s) 322, 931
BfaI CTAG 3 cut(s) 245, 965, 1058
BfoI RGCGCY 1 cut(s) 1208
BglI GCCNNNNNGGC 1 cut(s) 1061
BglII AGATCT 2 cut(s) 225, 619
BisI GCNGC 9 cut(s) 321, 375, 545, 711, 753, 879, 1107, 1203, 1279
BlsI GCNGC 9 cut(s) 322, 376, 546, 712, 754, 880, 1108, 1204, 1280
Bme1390I CCNGG 5 cut(s) 126, 426, 672, 819, 1296
BmeT110I CYCGRG 1 cut(s) 417
BmgT120I GGNCC 1 cut(s) 1280
BmiI GGNNCC 4 cut(s) 252, 823, 1005, 1093
BmrFI CCNGG 5 cut(s) 126, 426, 672, 819, 1296
BmrI ACTGGG 2 cut(s) 514, 1024
BmsI GCATC 2 cut(s) 463, 627
BmuI ACTGGG 2 cut(s) 514, 1024
BoxI GACNNNNGTC 1 cut(s) 597
BpiI GAAGAC 3 cut(s) 64, 597, 1238
BplI GAGNNNNNCTC 2 cut(s) 1267, 1299
BpmI CTGGAG 2 cut(s) 795, 995
BpuEI CTTGAG 1 cut(s) 1409
BpuMI CCSGG 3 cut(s) 426, 819, 1296
BsaAI YACGTR 2 cut(s) 412, 460
BsaBI GATNNNNATC 1 cut(s) 1070
BsaI GGTCTC 2 cut(s) 322, 931
BsaJI CCNNGG 7 cut(s) 124, 258, 425, 595, 671, 1126, 1294
BsaWI WCCGGW 2 cut(s) 767, 824
Bsc4I CCNNNNNNNGG 7 cut(s) 149, 345, 818, 824, 1088, 1300, 1418
Bse118I RCCGGY 3 cut(s) 278, 658, 824
Bse1I ACTGG 5 cut(s) 509, 978, 1019, 1166, 1412
Bse3DI GCAATG 1 cut(s) 699
Bse8I GATNNNNATC 1 cut(s) 1070
BseBI CCWGG 2 cut(s) 126, 672
BseDI CCNNGG 7 cut(s) 124, 258, 425, 595, 671, 1126, 1294
BseGI GGATG 4 cut(s) 478, 514, 1154, 1263
BseJI GATNNNNATC 1 cut(s) 1070
BseLI CCNNNNNNNGG 7 cut(s) 149, 345, 818, 824, 1088, 1300, 1418
BseMI GCAATG 1 cut(s) 699
BseMII CTCAG 1 cut(s) 425
BseNI ACTGG 5 cut(s) 509, 978, 1019, 1166, 1412
BseRI GAGGAG 1 cut(s) 208
BseXI GCAGC 8 cut(s) 332, 361, 556, 697, 764, 890, 1093, 1214
Bsh1236I CGCG 1 cut(s) 566
BshFI GGCC 4 cut(s) 142, 296, 899, 1281
BshNI GGYRCC 1 cut(s) 821
BshTI ACCGGT 1 cut(s) 824
BsiHKAI GWGCWC 2 cut(s) 424, 1175
BsiHKCI CYCGRG 1 cut(s) 417
BsiSI CCGG 9 cut(s) 279, 425, 659, 768, 818, 825, 839, 896, 1296
BslFI GGGAC 2 cut(s) 597, 688
BslI CCNNNNNNNGG 7 cut(s) 149, 345, 818, 824, 1088, 1300, 1418
BsmAI GTCTC 2 cut(s) 322, 931
BsmFI GGGAC 2 cut(s) 597, 688
BsnI GGCC 4 cut(s) 142, 296, 899, 1281
Bso31I GGTCTC 2 cut(s) 322, 931
BsoBI CYCGRG 1 cut(s) 417
Bsp1286I GDGCHC 3 cut(s) 424, 1175, 1425
Bsp143I GATC 6 cut(s) 225, 619, 814, 1071, 1219, 1386
Bsp19I CCATGG 1 cut(s) 595
Bsp68I TCGCGA 1 cut(s) 566
BspACI CCGC 3 cut(s) 355, 440, 1278
BspANI GGCC 4 cut(s) 142, 296, 899, 1281
BspCNI CTCAG 1 cut(s) 426
BspFNI CGCG 1 cut(s) 566
BspLI GGNNCC 4 cut(s) 252, 823, 1005, 1093
BspPI GGATC 3 cut(s) 809, 1079, 1214
BspQI GCTCTTC 2 cut(s) 356, 364
BspT107I GGYRCC 1 cut(s) 821
BspTNI GGTCTC 2 cut(s) 322, 931
BsrBI CCGCTC 3 cut(s) 357, 440, 1278
BsrDI GCAATG 1 cut(s) 699
BsrFI RCCGGY 3 cut(s) 278, 658, 824
BsrI ACTGG 5 cut(s) 509, 978, 1019, 1166, 1412
BssAI RCCGGY 3 cut(s) 278, 658, 824
BssECI CCNNGG 7 cut(s) 124, 258, 425, 595, 671, 1126, 1294
BssMI GATC 6 cut(s) 225, 619, 814, 1071, 1219, 1386
BssT1I CCWWGG 2 cut(s) 595, 1126
Bst2UI CCWGG 2 cut(s) 126, 672
Bst4CI ACNGT 4 cut(s) 259, 385, 679, 1168
Bst6I CTCTTC 5 cut(s) 356, 364, 740, 1089, 1206
BstAPI GCANNNNNTGC 1 cut(s) 758
BstBAI YACGTR 2 cut(s) 412, 460
BstC8I GCNNGC 2 cut(s) 642, 708
BstDEI CTNAG 3 cut(s) 434, 570, 1325
BstDSI CCRYGG 2 cut(s) 258, 595
BstF5I GGATG 4 cut(s) 478, 514, 1154, 1263
BstFNI CGCG 1 cut(s) 566
BstH2I RGCGCY 1 cut(s) 1208
BstHHI GCGC 1 cut(s) 1207
BstKTI GATC 6 cut(s) 228, 622, 817, 1074, 1222, 1389
BstMAI GTCTC 2 cut(s) 322, 931
BstMBI GATC 6 cut(s) 225, 619, 814, 1071, 1219, 1386
BstMWI GCNNNNNNNGC 4 cut(s) 698, 707, 758, 1061
BstNI CCWGG 2 cut(s) 126, 672
BstNSI RCATGY 1 cut(s) 1027
BstPAI GACNNNNGTC 1 cut(s) 597
BstSCI CCNGG 5 cut(s) 124, 424, 670, 817, 1294
BstUI CGCG 1 cut(s) 566
BstV1I GCAGC 8 cut(s) 332, 361, 556, 697, 764, 890, 1093, 1214
BstV2I GAAGAC 3 cut(s) 64, 597, 1238
BstX2I RGATCY 3 cut(s) 225, 619, 814
BstXI CCANNNNNNTGG 3 cut(s) 640, 1014, 1149
BstYI RGATCY 3 cut(s) 225, 619, 814
BsuRI GGCC 4 cut(s) 142, 296, 899, 1281
BtgI CCRYGG 2 cut(s) 258, 595
BtgZI GCGATG 3 cut(s) 491, 518, 1079
BtsCI GGATG 4 cut(s) 478, 514, 1154, 1263
BtsI GCAGTG 1 cut(s) 273
BtsIMutI CAGTG 4 cut(s) 273, 381, 1173, 1188
BtuMI TCGCGA 1 cut(s) 566
Cac8I GCNNGC 2 cut(s) 642, 708
CaiI CAGNNNCTG 1 cut(s) 677
CfoI GCGC 1 cut(s) 1207
Cfr10I RCCGGY 3 cut(s) 278, 658, 824
Cfr13I GGNCC 1 cut(s) 1280
CseI GACGC 1 cut(s) 750
Csp6I GTAC 2 cut(s) 502, 822
CspAI ACCGGT 1 cut(s) 824
CviQI GTAC 2 cut(s) 502, 822
DdeI CTNAG 3 cut(s) 434, 570, 1325
DpnI GATC 6 cut(s) 227, 621, 816, 1073, 1221, 1388
DpnII GATC 6 cut(s) 225, 619, 814, 1071, 1219, 1386
EaeI YGGCCR 2 cut(s) 140, 897
Eam1104I CTCTTC 5 cut(s) 356, 364, 740, 1089, 1206
EarI CTCTTC 5 cut(s) 356, 364, 740, 1089, 1206
Eco130I CCWWGG 2 cut(s) 595, 1126
Eco24I GRGCYC 1 cut(s) 1425
Eco31I GGTCTC 2 cut(s) 322, 931
Eco57I CTGAAG 1 cut(s) 1070
Eco88I CYCGRG 1 cut(s) 417
EcoRII CCWGG 2 cut(s) 124, 670
EcoT14I CCWWGG 2 cut(s) 595, 1126
EcoT38I GRGCYC 1 cut(s) 1425
ErhI CCWWGG 2 cut(s) 595, 1126
FaqI GGGAC 2 cut(s) 597, 688
FblI GTMKAC 2 cut(s) 764, 1158
Fnu4HI GCNGC 9 cut(s) 321, 375, 545, 711, 753, 879, 1107, 1203, 1279
FokI GGATG 4 cut(s) 485, 521, 1161, 1250
FriOI GRGCYC 1 cut(s) 1425
Fsp4HI GCNGC 9 cut(s) 321, 375, 545, 711, 753, 879, 1107, 1203, 1279
FspBI CTAG 3 cut(s) 245, 965, 1058
GlaI GCGC 1 cut(s) 1206
GluI GCNGC 9 cut(s) 321, 375, 545, 711, 753, 879, 1107, 1203, 1279
GsuI CTGGAG 2 cut(s) 795, 995
HaeII RGCGCY 1 cut(s) 1208
HaeIII GGCC 4 cut(s) 142, 296, 899, 1281
HapII CCGG 9 cut(s) 279, 425, 659, 768, 818, 825, 839, 896, 1296
HgaI GACGC 1 cut(s) 750
HhaI GCGC 1 cut(s) 1207
Hin6I GCGC 1 cut(s) 1205
HinP1I GCGC 1 cut(s) 1205
HindIII AAGCTT 1 cut(s) 611
HinfI GANTC 4 cut(s) 559, 585, 1353, 1402
HpaII CCGG 9 cut(s) 279, 425, 659, 768, 818, 825, 839, 896, 1296
HphI GGTGA 1 cut(s) 673
Hpy166II GTNNAC 3 cut(s) 765, 1159, 1372
Hpy188I TCNGA 5 cut(s) 100, 435, 624, 962, 1224
Hpy188III TCNNGA 6 cut(s) 417, 485, 556, 565, 812, 1273
Hpy8I GTNNAC 3 cut(s) 765, 1159, 1372
HpyAV CCTTC 4 cut(s) 128, 175, 1147, 1460
HpyCH4III ACNGT 4 cut(s) 259, 385, 679, 1168
HpyCH4IV ACGT 3 cut(s) 411, 459, 1050
HpyF10VI GCNNNNNNNGC 4 cut(s) 698, 707, 758, 1061
HpyF3I CTNAG 3 cut(s) 434, 570, 1325
HpySE526I ACGT 3 cut(s) 411, 459, 1050
HspAI GCGC 1 cut(s) 1205
KpnI GGTACC 1 cut(s) 825
Kzo9I GATC 6 cut(s) 225, 619, 814, 1071, 1219, 1386
LguI GCTCTTC 2 cut(s) 356, 364
LmnI GCTCC 3 cut(s) 250, 1003, 1275
Lsp1109I GCAGC 8 cut(s) 332, 361, 556, 697, 764, 890, 1093, 1214
LweI GCATC 2 cut(s) 463, 627
MaeI CTAG 3 cut(s) 245, 965, 1058
MaeII ACGT 3 cut(s) 411, 459, 1050
MaeIII GTNAC 1 cut(s) 281
MalI GATC 6 cut(s) 227, 621, 816, 1073, 1221, 1388
MbiI CCGCTC 3 cut(s) 357, 440, 1278
MboI GATC 6 cut(s) 225, 619, 814, 1071, 1219, 1386
MflI RGATCY 3 cut(s) 225, 619, 814
MhlI GDGCHC 3 cut(s) 424, 1175, 1425
MluCI AATT 6 cut(s) 287, 849, 861, 1137, 1287, 1320
MlyI GAGTC 2 cut(s) 553, 579
MmeI TCCRAC 2 cut(s) 985, 1247
MseI TTAA 2 cut(s) 738, 1459
MslI CAYNNNNRTG 2 cut(s) 635, 1181
MspI CCGG 9 cut(s) 279, 425, 659, 768, 818, 825, 839, 896, 1296
MspR9I CCNGG 5 cut(s) 126, 426, 672, 819, 1296
MvaI CCWGG 2 cut(s) 126, 672
MvnI CGCG 1 cut(s) 566
MwoI GCNNNNNNNGC 4 cut(s) 698, 707, 758, 1061
NciI CCSGG 3 cut(s) 426, 819, 1296
NcoI CCATGG 1 cut(s) 595
NdeII GATC 6 cut(s) 225, 619, 814, 1071, 1219, 1386
NlaIV GGNNCC 4 cut(s) 252, 823, 1005, 1093
NmuCI GTSAC 1 cut(s) 281
NruI TCGCGA 1 cut(s) 566
NspI RCATGY 1 cut(s) 1027
PaeR7I CTCGAG 1 cut(s) 417
PciSI GCTCTTC 2 cut(s) 356, 364
PcsI WCGNNNNNNNCGW 1 cut(s) 456
PfeI GAWTC 2 cut(s) 1353, 1402
PflMI CCANNNNNTGG 1 cut(s) 1418
PinAI ACCGGT 1 cut(s) 824
PkrI GCNGC 9 cut(s) 322, 376, 546, 712, 754, 880, 1108, 1204, 1280
PleI GAGTC 2 cut(s) 553, 579
PpsI GAGTC 2 cut(s) 553, 579
Ppu21I YACGTR 2 cut(s) 412, 460
PshAI GACNNNNGTC 1 cut(s) 597
PsiI TTATAA 2 cut(s) 87, 1349
Psp6I CCWGG 2 cut(s) 124, 670
PspGI CCWGG 2 cut(s) 124, 670
PspN4I GGNNCC 4 cut(s) 252, 823, 1005, 1093
PspPI GGNCC 1 cut(s) 1280
PstNI CAGNNNCTG 1 cut(s) 677
PsuI RGATCY 3 cut(s) 225, 619, 814
RruI TCGCGA 1 cut(s) 566
RsaI GTAC 2 cut(s) 503, 823
RsaNI GTAC 2 cut(s) 502, 822
RseI CAYNNNNRTG 2 cut(s) 635, 1181
SapI GCTCTTC 2 cut(s) 356, 364
SaqAI TTAA 2 cut(s) 738, 1459
SatI GCNGC 9 cut(s) 321, 375, 545, 711, 753, 879, 1107, 1203, 1279
Sau3AI GATC 6 cut(s) 225, 619, 814, 1071, 1219, 1386
Sau96I GGNCC 1 cut(s) 1280
SchI GAGTC 2 cut(s) 553, 579
ScrFI CCNGG 5 cut(s) 126, 426, 672, 819, 1296
SduI GDGCHC 3 cut(s) 424, 1175, 1425
SfaNI GCATC 2 cut(s) 463, 627
Sfr274I CTCGAG 1 cut(s) 417
SlaI CTCGAG 1 cut(s) 417
SmiMI CAYNNNNRTG 2 cut(s) 635, 1181
SmlI CTYRAG 2 cut(s) 417, 1424
SmoI CTYRAG 2 cut(s) 417, 1424
Sse9I AATT 6 cut(s) 287, 849, 861, 1137, 1287, 1320
SsiI CCGC 3 cut(s) 355, 440, 1278
SspI AATATT 2 cut(s) 667, 1196
SspMI CTAG 3 cut(s) 245, 965, 1058
StyD4I CCNGG 5 cut(s) 124, 424, 670, 817, 1294
StyI CCWWGG 2 cut(s) 595, 1126
TaaI ACNGT 4 cut(s) 259, 385, 679, 1168
TaiI ACGT 3 cut(s) 414, 462, 1053
TaqI TCGA 6 cut(s) 418, 486, 588, 688, 1218, 1285
TasI AATT 6 cut(s) 287, 849, 861, 1137, 1287, 1320
TatI WGTACW 1 cut(s) 501
TauI GCSGC 1 cut(s) 1281
TfiI GAWTC 2 cut(s) 1353, 1402
Tru1I TTAA 2 cut(s) 738, 1459
Tru9I TTAA 2 cut(s) 738, 1459
TscAI CASTG 4 cut(s) 280, 388, 1173, 1195
TseFI GTSAC 1 cut(s) 281
TseI GCWGC 8 cut(s) 320, 374, 544, 710, 752, 878, 1106, 1202
Tsp45I GTSAC 1 cut(s) 281
TspRI CASTG 4 cut(s) 280, 388, 1173, 1195
Van91I CCANNNNNTGG 1 cut(s) 1418
XapI RAATTY 3 cut(s) 287, 1287, 1320
XceI RCATGY 1 cut(s) 1027
XhoI CTCGAG 1 cut(s) 417
XmiI GTMKAC 2 cut(s) 764, 1158
XspI CTAG 3 cut(s) 245, 965, 1058
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.