Rorug03G0371300

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0000778
Physical Location & Seq
Forward (+)
45515383 .. 45523060
7678 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0371300.1

Sequence Viewer

Length: 1671 bp
ATGTCGATCCAAGTTGGTCCGACTGTTGAGCTAGAAAACGCCATGCCTGAAATTGCTCATCCAAAGACAAGTTTTCAACCAGGCATTTCCATAGATGGGTTCATCAACTGTCATTCCCTCGACAGCATAACTCATGCTCGTATGCAACAGCAAATTGACGAACTGAAAGTACTAGTGAAGGAAGAAGTTTTCGCATCATATGATGTTTCATATCAGTTGAAATTAATTGATGAAATCCAACGCCTAGGTGTGGCATACCATTTTGAAACAGAAATAGATGAAGCACTAGAAAATATCTATGTTAAATACCATGATGATGGTGATCTGGACCTATATAATGTTTCTCTTCGTTTTCGTCTACTAAGACAAAATGGATATAATGTTTCATCTGATATATTCAGCAAATTTAAAGATGTAAACAGTTACTTCAAGGAGAGCTTAATTGCTGATATCTTAGCTATCATATGCTTGTATGAGGCAACACATCTTAGGGTTCATGGAGAAGAAATACTAGAAGAAGCTCTTGTTTTCACCACCACCCACCTTGAATTGGCGATAAGTGGTGTAAGCTATCCACTAGCTGCAAAAATATCTCAAGCCCTAGAGAGGCCTCTGCGTAAAGGTCTAGAGAGGTTATGTACCAGGAATTACATACCAATCTACGAAGCCACGACTCCGCATAACGAAGCGCTACTGAAACTTGCGAAGTTAGATTTCAATCTGGTTCAATCTTTGCACAAAGAGGAGCTTAGTGAGGTTTCAAGGTGGGGGAAAGAACTAGGCTTTGAAAAGAATCTTCCTTTTGTAAGGTATAGGATTGTGGAGTGTTTCTTTTGGATGGTGGGAATGTATTTTGAACCCCGATACTCGGTTGGAAGAATAATCGGAACAAAACTGTGTGTTCTAGCTACAATTTTGGATGATATCTTTGATGCATGTGGTACACTTGAAGAACTCAAGATATTTCGTGAAGCAGTTGACAGGTTTGATGTCAACTATTGCACAAATGGTCTACCACGGTACATGCAAGTATACTATCATTTACTTTTGAATACTATGAATGAAATTGAGGAAGAGCTAGAAAAGCAAGGAATATCGTACCGAGTCCACTACGCAAAACAAGCTTTGAAGAATCAAGCTAGAGACTACCTTATTGAGGCCCAATGGCTCCATGAAGGATGTATCCCTTGCATGGAGGAGTATATGCATGTCAGATTGACTTCTGTTGGTGTTGCTATGACTGTAGTCTTTTCTTTAATTGGAATGGAAGAAACTATTACCGAGGAAACATTTGAGTGGATTTTGAAGTACCCGAAAAGTATTAGGGCTTCGAGCCTTATCTTTAGGCTCATGGATGACATTGCGGGGAGTAAGCATAAGAAAGAAAAAGGGGATGTTGCTTCTAGTATTGAATGTTACATGAAGCAATATGAGGTCTCAGAGAAAGAGACTATCGATGTGTTTAACAAACAAATTATAGATGCATGGAAGGACATAAATGAGGATTTACTTCGACCAACTGTTGTGCCAATGTGTGCGATTAAGCTCGGTCTTAATTTCGCAAGAGGTGTTGATCTGGTTTACAAAGAAGAAGATGAATTCACATATGTTGGAGAAGTAGTTAAACGCTCTGTCGCTGCACTTTTTGTTGATCCATTGCCACTCGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
p450

Protein Analysis

556

Amino Acids

64.16

Weight (kDa)

5.05

Isoelectric Point (pI)

42.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Terpene_synth PF01397 42 - 201 6.8e-51 Terpene synthase, N-terminal domain
Terpene_synth_C PF03936 258 - 498 3.7e-89 Terpene synthase family, metal binding domain
Terpene_syn_C_2 PF19086 298 - 498 9e-52 Terpene synthase family 2, C-terminal metal binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 3 cut(s) 358, 1012, 1032
AciI CCGC 2 cut(s) 677, 1364
AclWI GGATC 1 cut(s) 1646
AcsI RAATTY 2 cut(s) 404, 1598
AfaI GTAC 6 cut(s) 171, 640, 943, 1022, 1100, 1310
AfeI AGCGCT 1 cut(s) 690
AfiI CCNNNNNNNGG 7 cut(s) 96, 250, 550, 606, 868, 1156, 1192
AhlI ACTAGT 1 cut(s) 172
AjnI CCWGG 2 cut(s) 79, 641
Alw26I GTCTC 3 cut(s) 1137, 1441, 1442
AlwI GGATC 1 cut(s) 1646
Aor51HI AGCGCT 1 cut(s) 690
AoxI GGCC 2 cut(s) 608, 1158
ApeKI GCWGC 2 cut(s) 581, 1637
ApoI RAATTY 2 cut(s) 404, 1598
AseI ATTAAT 1 cut(s) 224
AspA2I CCTAGG 1 cut(s) 244
AspLEI GCGC 1 cut(s) 691
AspS9I GGNCC 3 cut(s) 17, 328, 1159
AsuHPI GGTGA 2 cut(s) 332, 523
AvaII GGWCC 2 cut(s) 17, 328
AvrII CCTAGG 1 cut(s) 244
BarI GAAGNNNNNNTAC 2 cut(s) 1312, 1344
BbvI GCAGC 2 cut(s) 568, 1624
BccI CCATC 3 cut(s) 89, 311, 832
BciT130I CCWGG 2 cut(s) 81, 643
BciVI GTATCC 1 cut(s) 1193
BcoDI GTCTC 3 cut(s) 1137, 1441, 1442
BcuI ACTAGT 1 cut(s) 172
BfmI CTRYAG 1 cut(s) 1242
BfoI RGCGCY 1 cut(s) 692
BfuI GTATCC 1 cut(s) 1193
BisI GCNGC 2 cut(s) 582, 1638
BlnI CCTAGG 1 cut(s) 244
BlsI GCNGC 2 cut(s) 583, 1639
BmcAI AGTACT 1 cut(s) 171
Bme1390I CCNGG 2 cut(s) 81, 643
Bme18I GGWCC 2 cut(s) 17, 328
BmgT120I GGNCC 3 cut(s) 17, 328, 1159
BmiI GGNNCC 1 cut(s) 1169
BmrFI CCNGG 2 cut(s) 81, 643
BmsI GCATC 3 cut(s) 203, 922, 1471
BoxI GACNNNNGTC 1 cut(s) 1244
BpuEI CTTGAG 2 cut(s) 579, 941
Bsa29I ATCGAT 1 cut(s) 1455
BsaBI GATNNNNATC 2 cut(s) 321, 717
BsaI GGTCTC 1 cut(s) 1441
BsaJI CCNNGG 3 cut(s) 244, 1016, 1281
BsaXI ACNNNNNCTCC 2 cut(s) 1605, 1635
Bsc4I CCNNNNNNNGG 7 cut(s) 96, 250, 550, 606, 868, 1156, 1192
Bse3DI GCAATG 2 cut(s) 1359, 1655
Bse8I GATNNNNATC 2 cut(s) 321, 717
BseBI CCWGG 2 cut(s) 81, 643
BseCI ATCGAT 1 cut(s) 1455
BseDI CCNNGG 3 cut(s) 244, 1016, 1281
BseGI GGATG 6 cut(s) 58, 843, 925, 1184, 1360, 1399
BseJI GATNNNNATC 2 cut(s) 321, 717
BseLI CCNNNNNNNGG 7 cut(s) 96, 250, 550, 606, 868, 1156, 1192
BseMI GCAATG 2 cut(s) 1359, 1655
BseMII CTCAG 1 cut(s) 1452
BseRI GAGGAG 2 cut(s) 758, 1211
BseXI GCAGC 2 cut(s) 568, 1624
BsgI GTGCAG 1 cut(s) 1623
BshFI GGCC 2 cut(s) 610, 1160
BshVI ATCGAT 1 cut(s) 1455
BslI CCNNNNNNNGG 7 cut(s) 96, 250, 550, 606, 868, 1156, 1192
BsmAI GTCTC 3 cut(s) 1137, 1441, 1442
BsnI GGCC 2 cut(s) 610, 1160
Bso31I GGTCTC 1 cut(s) 1441
Bsp143I GATC 4 cut(s) 6, 322, 1573, 1651
BspACI CCGC 2 cut(s) 677, 1364
BspANI GGCC 2 cut(s) 610, 1160
BspCNI CTCAG 1 cut(s) 1451
BspDI ATCGAT 1 cut(s) 1455
BspLI GGNNCC 1 cut(s) 1169
BspPI GGATC 1 cut(s) 1646
BspQI GCTCTTC 1 cut(s) 1068
BspTNI GGTCTC 1 cut(s) 1441
BsrDI GCAATG 2 cut(s) 1359, 1655
BssECI CCNNGG 3 cut(s) 244, 1016, 1281
BssMI GATC 4 cut(s) 6, 322, 1573, 1651
BssNAI GTATAC 1 cut(s) 1033
BssT1I CCWWGG 1 cut(s) 244
Bst1107I GTATAC 1 cut(s) 1033
Bst2UI CCWGG 2 cut(s) 81, 643
Bst4CI ACNGT 7 cut(s) 25, 110, 422, 897, 1020, 1243, 1522
Bst6I CTCTTC 2 cut(s) 351, 1068
BstDEI CTNAG 5 cut(s) 362, 454, 488, 749, 1438
BstDSI CCRYGG 1 cut(s) 1016
BstENI CCTNNNNNAGG 1 cut(s) 1154
BstF5I GGATG 6 cut(s) 58, 843, 925, 1184, 1360, 1399
BstH2I RGCGCY 1 cut(s) 692
BstHHI GCGC 1 cut(s) 691
BstKTI GATC 4 cut(s) 9, 325, 1576, 1654
BstMAI GTCTC 3 cut(s) 1137, 1441, 1442
BstMBI GATC 4 cut(s) 6, 322, 1573, 1651
BstMWI GCNNNNNNNGC 2 cut(s) 1084, 1121
BstNI CCWGG 2 cut(s) 81, 643
BstNSI RCATGY 3 cut(s) 939, 1027, 1211
BstPAI GACNNNNGTC 1 cut(s) 1244
BstSCI CCNGG 2 cut(s) 79, 641
BstSFI CTRYAG 1 cut(s) 1242
BstV1I GCAGC 2 cut(s) 568, 1624
BstXI CCANNNNNNTGG 1 cut(s) 317
BstZ17I GTATAC 1 cut(s) 1033
Bsu15I ATCGAT 1 cut(s) 1455
BsuI GTATCC 1 cut(s) 1193
BsuRI GGCC 2 cut(s) 610, 1160
BsuTUI ATCGAT 1 cut(s) 1455
BtgI CCRYGG 1 cut(s) 1016
BtsCI GGATG 6 cut(s) 58, 843, 925, 1184, 1360, 1399
CfoI GCGC 1 cut(s) 691
Cfr13I GGNCC 3 cut(s) 17, 328, 1159
ClaI ATCGAT 1 cut(s) 1455
Csp6I GTAC 6 cut(s) 170, 639, 942, 1021, 1099, 1309
CspCI CAANNNNNGTGG 2 cut(s) 526, 561
CviQI GTAC 6 cut(s) 170, 639, 942, 1021, 1099, 1309
DdeI CTNAG 5 cut(s) 362, 454, 488, 749, 1438
DpnI GATC 4 cut(s) 8, 324, 1575, 1653
DpnII GATC 4 cut(s) 6, 322, 1573, 1651
DraI TTTAAA 1 cut(s) 409
Eam1104I CTCTTC 2 cut(s) 351, 1068
EarI CTCTTC 2 cut(s) 351, 1068
Eco130I CCWWGG 1 cut(s) 244
Eco147I AGGCCT 1 cut(s) 610
Eco31I GGTCTC 1 cut(s) 1441
Eco32I GATATC 2 cut(s) 451, 925
Eco47I GGWCC 2 cut(s) 17, 328
Eco47III AGCGCT 1 cut(s) 690
EcoNI CCTNNNNNAGG 1 cut(s) 1154
EcoRI GAATTC 1 cut(s) 1598
EcoRII CCWGG 2 cut(s) 79, 641
EcoRV GATATC 2 cut(s) 451, 925
EcoT14I CCWWGG 1 cut(s) 244
EcoT22I ATGCAT 3 cut(s) 937, 1209, 1486
ErhI CCWWGG 1 cut(s) 244
FalI AAGNNNNNCTT 6 cut(s) 422, 454, 507, 539, 732, 764
FauI CCCGC 1 cut(s) 1357
FauNDI CATATG 3 cut(s) 199, 464, 1606
FblI GTMKAC 3 cut(s) 358, 1012, 1032
Fnu4HI GCNGC 2 cut(s) 582, 1638
FokI GGATG 6 cut(s) 45, 850, 932, 1191, 1367, 1406
Fsp4HI GCNGC 2 cut(s) 582, 1638
GlaI GCGC 1 cut(s) 690
GluI GCNGC 2 cut(s) 582, 1638
HaeII RGCGCY 1 cut(s) 692
HaeIII GGCC 2 cut(s) 610, 1160
HhaI GCGC 1 cut(s) 691
Hin6I GCGC 1 cut(s) 689
HinP1I GCGC 1 cut(s) 689
HincII GTYRAC 2 cut(s) 979, 994
HindII GTYRAC 2 cut(s) 979, 994
HindIII AAGCTT 1 cut(s) 1122
HinfI GANTC 4 cut(s) 673, 793, 1104, 1132
HphI GGTGA 2 cut(s) 332, 523
Hpy166II GTNNAC 9 cut(s) 359, 418, 944, 979, 994, 1013, 1033, 1108, 1582
Hpy188I TCNGA 5 cut(s) 21, 391, 887, 1214, 1441
Hpy188III TCNNGA 4 cut(s) 326, 626, 958, 968
Hpy8I GTNNAC 9 cut(s) 359, 418, 944, 979, 994, 1013, 1033, 1108, 1582
HpyAV CCTTC 3 cut(s) 172, 1169, 1483
HpyCH4III ACNGT 7 cut(s) 25, 110, 422, 897, 1020, 1243, 1522
HpyF10VI GCNNNNNNNGC 2 cut(s) 1084, 1121
HpyF3I CTNAG 5 cut(s) 362, 454, 488, 749, 1438
HspAI GCGC 1 cut(s) 689
Kzo9I GATC 4 cut(s) 6, 322, 1573, 1651
LguI GCTCTTC 1 cut(s) 1068
LmnI GCTCC 2 cut(s) 745, 1173
LpnPI CCDG 9 cut(s) 60, 66, 93, 311, 628, 655, 707, 967, 1562
Lsp1109I GCAGC 2 cut(s) 568, 1624
LweI GCATC 3 cut(s) 203, 922, 1471
MaeIII GTNAC 2 cut(s) 422, 1415
MalI GATC 4 cut(s) 8, 324, 1575, 1653
MboI GATC 4 cut(s) 6, 322, 1573, 1651
MlyI GAGTC 2 cut(s) 667, 1113
MmeI TCCRAC 4 cut(s) 44, 262, 853, 1591
Mph1103I ATGCAT 3 cut(s) 937, 1209, 1486
MseI TTAA 9 cut(s) 224, 303, 408, 440, 1256, 1464, 1542, 1554, 1623
MslI CAYNNNNRTG 3 cut(s) 315, 1294, 1666
MspR9I CCNGG 2 cut(s) 81, 643
MvaI CCWGG 2 cut(s) 81, 643
MwoI GCNNNNNNNGC 2 cut(s) 1084, 1121
NdeI CATATG 3 cut(s) 199, 464, 1606
NdeII GATC 4 cut(s) 6, 322, 1573, 1651
NlaIV GGNNCC 1 cut(s) 1169
NsiI ATGCAT 3 cut(s) 937, 1209, 1486
NspI RCATGY 3 cut(s) 939, 1027, 1211
PceI AGGCCT 1 cut(s) 610
PciSI GCTCTTC 1 cut(s) 1068
PfeI GAWTC 2 cut(s) 793, 1132
PkrI GCNGC 2 cut(s) 583, 1639
PleI GAGTC 2 cut(s) 667, 1112
PpsI GAGTC 2 cut(s) 667, 1112
PshAI GACNNNNGTC 1 cut(s) 1244
PshBI ATTAAT 1 cut(s) 224
Psp6I CCWGG 2 cut(s) 79, 641
PspGI CCWGG 2 cut(s) 79, 641
PspN4I GGNNCC 1 cut(s) 1169
PspPI GGNCC 3 cut(s) 17, 328, 1159
PsrI GAACNNNNNNTAC 2 cut(s) 153, 185
RsaI GTAC 6 cut(s) 171, 640, 943, 1022, 1100, 1310
RsaNI GTAC 6 cut(s) 170, 639, 942, 1021, 1099, 1309
RseI CAYNNNNRTG 3 cut(s) 315, 1294, 1666
SapI GCTCTTC 1 cut(s) 1068
SaqAI TTAA 9 cut(s) 224, 303, 408, 440, 1256, 1464, 1542, 1554, 1623
SatI GCNGC 2 cut(s) 582, 1638
Sau3AI GATC 4 cut(s) 6, 322, 1573, 1651
Sau96I GGNCC 3 cut(s) 17, 328, 1159
ScaI AGTACT 1 cut(s) 171
SchI GAGTC 2 cut(s) 667, 1113
ScrFI CCNGG 2 cut(s) 81, 643
SfaNI GCATC 3 cut(s) 203, 922, 1471
SfcI CTRYAG 1 cut(s) 1242
SinI GGWCC 2 cut(s) 17, 328
SmiMI CAYNNNNRTG 3 cut(s) 315, 1294, 1666
SmlI CTYRAG 2 cut(s) 594, 956
SmoI CTYRAG 2 cut(s) 594, 956
SpeI ACTAGT 1 cut(s) 172
SseBI AGGCCT 1 cut(s) 610
SsiI CCGC 2 cut(s) 677, 1364
StuI AGGCCT 1 cut(s) 610
StyD4I CCNGG 2 cut(s) 79, 641
StyI CCWWGG 1 cut(s) 244
TaaI ACNGT 7 cut(s) 25, 110, 422, 897, 1020, 1243, 1522
TaqI TCGA 6 cut(s) 5, 120, 1331, 1455, 1513, 1665
TaqII GACCGA 1 cut(s) 1538
TatI WGTACW 1 cut(s) 169
TfiI GAWTC 2 cut(s) 793, 1132
Tru1I TTAA 9 cut(s) 224, 303, 408, 440, 1256, 1464, 1542, 1554, 1623
Tru9I TTAA 9 cut(s) 224, 303, 408, 440, 1256, 1464, 1542, 1554, 1623
TseI GCWGC 2 cut(s) 581, 1637
VpaK11BI GGWCC 2 cut(s) 17, 328
VspI ATTAAT 1 cut(s) 224
XagI CCTNNNNNAGG 1 cut(s) 1154
XapI RAATTY 2 cut(s) 404, 1598
XbaI TCTAGA 1 cut(s) 625
XceI RCATGY 3 cut(s) 939, 1027, 1211
XmaJI CCTAGG 1 cut(s) 244
XmiI GTMKAC 3 cut(s) 358, 1012, 1032
ZrmI AGTACT 1 cut(s) 171
Zsp2I ATGCAT 3 cut(s) 937, 1209, 1486
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.