RchiOBHm_Chr4g0393751

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0000778
Physical Location & Seq
Forward (+)
9308182 .. 9310306
2125 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ36629

Sequence Viewer

Length: 1008 bp
ATGGTTCTCGACCACCTGAAAACGCACTGGGATGGCAAACAGGTGGTCGAGGCTCATCGCCTCACGCAGCTACTTGTTTTGACTCTCTCGGCCAAGTTCTTCATGGGACTCGAAGACAGTTGTCGTATTGGTAAGCTTGTGGAGTTGATGGACACCATGATGCTGGCGCTTCACACTATTCCTGTGAATATTCCGGGGACTGCATTTCATCGAGCAATGAAAGCAGCGCCGGCTGCGAGGGAGATTATTCAGTCGTTTGTTAAGGAGAAGAAAGAAGTTATGGAGTCTACCGGAAACAAAATGCATGATCTTTTGTCTTATATGATTGCTACGCCAGACCCAACCGGAAGGTTCATGCCGGAGAATGAAATTGCTGATAAAATTATGGGGAATGTGGCCGCTTCGTTCAACTCTCCAGCTATGACTACTACTTTTATCGTGAAGTTCCTGGGCGAAAGACCTGACATATATGACAAAGTCCGAATTGAACAACTGGAGATTTTGAAGTCCAAAAAATCTGGCGAGGCACTCAATTGGGATGATATGCAAAAAATGAAGTATTCGTGGAACGTAGCCCTGGAGGCGATGAGGCTCATACCACCTCTTCAGGGAACATTTAGAGAGGCTGCAACAGACTTCACTTACCAAGGTTATACAATTCCTAAAGGATGGAAGGTCTACTGGACAGTGAGCACCACAACCATGAACCCTGAATATTTTCCAGCGCCAGAAGAGTTTGACCCTACAAGGTTCGAGAAAGCAACTCCTCCGCCTTACACAAACATCCCATTCGGTAGTGGTCCTCGAATTTGCCCCGGAAAGGACTACGCTCGTTTGCAAATTCTTAGTTTTATGCACCATCTTGTTATAAGATTCAAATGGGACCTTGTGAACCCTAATTGCAAGATCACAGGTGGTATGAATCCCTTTCCAGTTGATGGGCTGAAGGTTCGTCTCCAAGCTCAACCCAGTCAAGCTTTAGTTGTACTACGCACGTACGTAGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
p450

Protein Analysis

335

Amino Acids

38.08

Weight (kDa)

8.57

Isoelectric Point (pI)

38.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 45 - 298 5.2e-49 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 869
AccB7I CCANNNNNTGG 1 cut(s) 938
AccI GTMKAC 2 cut(s) 287, 678
AciI CCGC 2 cut(s) 399, 770
AcoI YGGCCR 2 cut(s) 90, 396
AcsI RAATTY 2 cut(s) 807, 840
AcuI CTGAAG 2 cut(s) 590, 965
AfaI GTAC 2 cut(s) 987, 998
AfiI CCNNNNNNNGG 3 cut(s) 608, 820, 938
AgsI TTSAA 4 cut(s) 409, 488, 505, 877
AjnI CCWGG 2 cut(s) 447, 576
AluBI AGCT 5 cut(s) 70, 136, 419, 962, 977
AluI AGCT 5 cut(s) 70, 136, 419, 962, 977
Alw21I GWGCWC 1 cut(s) 695
Alw26I GTCTC 1 cut(s) 959
AoxI GGCC 2 cut(s) 90, 396
ApeKI GCWGC 4 cut(s) 67, 224, 233, 626
ApoI RAATTY 2 cut(s) 807, 840
ArsI GACNNNNNNTTYG 2 cut(s) 30, 62
Asp700I GAANNNNTTC 1 cut(s) 717
AspLEI GCGC 3 cut(s) 169, 229, 727
AspS9I GGNCC 2 cut(s) 800, 883
AsuC2I CCSGG 2 cut(s) 195, 816
AvaII GGWCC 2 cut(s) 800, 883
BbsI GAAGAC 1 cut(s) 120
Bbv12I GWGCWC 1 cut(s) 695
BbvI GCAGC 4 cut(s) 79, 220, 236, 613
BccI CCATC 5 cut(s) 26, 142, 663, 867, 932
BciT130I CCWGG 2 cut(s) 449, 578
BcnI CCSGG 2 cut(s) 195, 816
BcoDI GTCTC 1 cut(s) 959
BfoI RGCGCY 3 cut(s) 170, 230, 728
BglI GCCNNNNNGGC 1 cut(s) 581
BisI GCNGC 5 cut(s) 68, 225, 234, 399, 627
BlsI GCNGC 5 cut(s) 69, 226, 235, 400, 628
Bme1390I CCNGG 4 cut(s) 195, 449, 578, 816
Bme18I GGWCC 2 cut(s) 800, 883
BmgT120I GGNCC 2 cut(s) 800, 883
BmiI GGNNCC 1 cut(s) 884
BmrFI CCNGG 4 cut(s) 195, 449, 578, 816
BmrI ACTGGG 2 cut(s) 37, 963
BmsI GCATC 1 cut(s) 150
BmuI ACTGGG 2 cut(s) 37, 963
BoxI GACNNNNGTC 1 cut(s) 120
BpiI GAAGAC 1 cut(s) 120
BpmI CTGGAG 3 cut(s) 399, 515, 599
BpuMI CCSGG 2 cut(s) 195, 816
BsaAI YACGTR 2 cut(s) 996, 1000
BsaJI CCNNGG 5 cut(s) 194, 448, 576, 646, 814
BsaWI WCCGGW 2 cut(s) 290, 344
Bsc4I CCNNNNNNNGG 3 cut(s) 608, 820, 938
Bse118I RCCGGY 1 cut(s) 229
Bse1I ACTGG 5 cut(s) 32, 498, 686, 932, 969
Bse3DI GCAATG 1 cut(s) 222
BseBI CCWGG 2 cut(s) 449, 578
BseDI CCNNGG 5 cut(s) 194, 448, 576, 646, 814
BseGI GGATG 4 cut(s) 37, 544, 674, 783
BseLI CCNNNNNNNGG 3 cut(s) 608, 820, 938
BseMI GCAATG 1 cut(s) 222
BseNI ACTGG 5 cut(s) 32, 498, 686, 932, 969
BseRI GAGGAG 1 cut(s) 756
BseXI GCAGC 4 cut(s) 79, 220, 236, 613
BshFI GGCC 2 cut(s) 92, 398
BsiHKAI GWGCWC 1 cut(s) 695
BsiSI CCGG 6 cut(s) 194, 230, 291, 345, 359, 816
BsiWI CGTACG 1 cut(s) 996
BslFI GGGAC 3 cut(s) 120, 211, 896
BslI CCNNNNNNNGG 3 cut(s) 608, 820, 938
BsmAI GTCTC 1 cut(s) 959
BsmBI CGTCTC 1 cut(s) 959
BsmFI GGGAC 3 cut(s) 120, 211, 896
BsnI GGCC 2 cut(s) 92, 398
Bsp1286I GDGCHC 1 cut(s) 695
Bsp143I GATC 2 cut(s) 307, 906
BspACI CCGC 2 cut(s) 399, 770
BspANI GGCC 2 cut(s) 92, 398
BspLI GGNNCC 1 cut(s) 884
BsrDI GCAATG 1 cut(s) 222
BsrFI RCCGGY 1 cut(s) 229
BsrI ACTGG 5 cut(s) 32, 498, 686, 932, 969
BssAI RCCGGY 1 cut(s) 229
BssECI CCNNGG 5 cut(s) 194, 448, 576, 646, 814
BssMI GATC 2 cut(s) 307, 906
BssT1I CCWWGG 1 cut(s) 646
Bst2UI CCWGG 2 cut(s) 449, 578
Bst4CI ACNGT 2 cut(s) 119, 688
Bst6I CTCTTC 2 cut(s) 609, 726
BstBAI YACGTR 2 cut(s) 996, 1000
BstC8I GCNNGC 2 cut(s) 165, 231
BstDEI CTNAG 1 cut(s) 845
BstF5I GGATG 4 cut(s) 37, 544, 674, 783
BstH2I RGCGCY 3 cut(s) 170, 230, 728
BstHHI GCGC 3 cut(s) 169, 229, 727
BstKTI GATC 2 cut(s) 310, 909
BstMAI GTCTC 1 cut(s) 959
BstMBI GATC 2 cut(s) 307, 906
BstMWI GCNNNNNNNGC 4 cut(s) 221, 230, 233, 581
BstNI CCWGG 2 cut(s) 449, 578
BstPAI GACNNNNGTC 1 cut(s) 120
BstSCI CCNGG 4 cut(s) 193, 447, 576, 814
BstSNI TACGTA 1 cut(s) 1000
BstV1I GCAGC 4 cut(s) 79, 220, 236, 613
BstV2I GAAGAC 1 cut(s) 120
BstXI CCANNNNNNTGG 1 cut(s) 163
BsuRI GGCC 2 cut(s) 92, 398
BtgZI GCGATG 2 cut(s) 41, 599
BtsCI GGATG 4 cut(s) 37, 544, 674, 783
BtsIMutI CAGTG 2 cut(s) 25, 693
Cac8I GCNNGC 2 cut(s) 165, 231
CfoI GCGC 3 cut(s) 169, 229, 727
Cfr10I RCCGGY 1 cut(s) 229
Cfr13I GGNCC 2 cut(s) 800, 883
Csp6I GTAC 2 cut(s) 986, 997
CviAII CATG 5 cut(s) 103, 157, 305, 355, 703
CviQI GTAC 2 cut(s) 986, 997
DdeI CTNAG 1 cut(s) 845
DpnI GATC 2 cut(s) 309, 908
DpnII GATC 2 cut(s) 307, 906
EaeI YGGCCR 2 cut(s) 90, 396
Eam1104I CTCTTC 2 cut(s) 609, 726
EarI CTCTTC 2 cut(s) 609, 726
EciI GGCGGA 1 cut(s) 759
Eco105I TACGTA 1 cut(s) 1000
Eco130I CCWWGG 1 cut(s) 646
Eco47I GGWCC 2 cut(s) 800, 883
Eco57I CTGAAG 2 cut(s) 590, 965
EcoO109I RGGNCCY 1 cut(s) 883
EcoRII CCWGG 2 cut(s) 447, 576
EcoT14I CCWWGG 1 cut(s) 646
EcoT22I ATGCAT 1 cut(s) 306
ErhI CCWWGG 1 cut(s) 646
Esp3I CGTCTC 1 cut(s) 959
FaeI CATG 5 cut(s) 106, 160, 308, 358, 706
FaqI GGGAC 3 cut(s) 120, 211, 896
FatI CATG 5 cut(s) 102, 156, 304, 354, 702
FblI GTMKAC 2 cut(s) 287, 678
Fnu4HI GCNGC 5 cut(s) 68, 225, 234, 399, 627
FokI GGATG 4 cut(s) 44, 551, 681, 770
Fsp4HI GCNGC 5 cut(s) 68, 225, 234, 399, 627
GlaI GCGC 3 cut(s) 168, 228, 726
GluI GCNGC 5 cut(s) 68, 225, 234, 399, 627
GsuI CTGGAG 3 cut(s) 399, 515, 599
HaeII RGCGCY 3 cut(s) 170, 230, 728
HaeIII GGCC 2 cut(s) 92, 398
HapII CCGG 6 cut(s) 194, 230, 291, 345, 359, 816
HhaI GCGC 3 cut(s) 169, 229, 727
Hin1II CATG 5 cut(s) 106, 160, 308, 358, 706
Hin6I GCGC 3 cut(s) 167, 227, 725
HinP1I GCGC 3 cut(s) 167, 227, 725
HindIII AAGCTT 2 cut(s) 134, 975
HinfI GANTC 5 cut(s) 82, 108, 284, 873, 922
HpaII CCGG 6 cut(s) 194, 230, 291, 345, 359, 816
Hpy166II GTNNAC 3 cut(s) 288, 679, 892
Hpy188I TCNGA 1 cut(s) 482
Hpy188III TCNNGA 3 cut(s) 8, 439, 754
Hpy8I GTNNAC 3 cut(s) 288, 679, 892
HpyAV CCTTC 3 cut(s) 342, 667, 940
HpyCH4III ACNGT 2 cut(s) 119, 688
HpyCH4IV ACGT 3 cut(s) 570, 995, 999
HpyCH4V TGCA 7 cut(s) 203, 304, 547, 629, 838, 856, 903
HpyF10VI GCNNNNNNNGC 4 cut(s) 221, 230, 233, 581
HpyF3I CTNAG 1 cut(s) 845
HpySE526I ACGT 3 cut(s) 570, 995, 999
Hsp92II CATG 5 cut(s) 106, 160, 308, 358, 706
HspAI GCGC 3 cut(s) 167, 227, 725
KroI GCCGGC 1 cut(s) 229
KroNI GCCGGC 1 cut(s) 231
Kzo9I GATC 2 cut(s) 307, 906
Lsp1109I GCAGC 4 cut(s) 79, 220, 236, 613
LweI GCATC 1 cut(s) 150
MaeII ACGT 3 cut(s) 570, 995, 999
MalI GATC 2 cut(s) 309, 908
MboI GATC 2 cut(s) 307, 906
MboII GAAGA 5 cut(s) 91, 125, 280, 596, 743
MfeI CAATTG 1 cut(s) 532
MhlI GDGCHC 1 cut(s) 695
MluCI AATT 8 cut(s) 369, 381, 483, 532, 657, 807, 840, 898
MlyI GAGTC 3 cut(s) 76, 102, 293
Mph1103I ATGCAT 1 cut(s) 306
MroNI GCCGGC 1 cut(s) 229
MroXI GAANNNNTTC 1 cut(s) 717
MseI TTAA 1 cut(s) 261
MslI CAYNNNNRTG 3 cut(s) 30, 158, 701
MspI CCGG 6 cut(s) 194, 230, 291, 345, 359, 816
MspR9I CCNGG 4 cut(s) 195, 449, 578, 816
MunI CAATTG 1 cut(s) 532
MvaI CCWGG 2 cut(s) 449, 578
MwoI GCNNNNNNNGC 4 cut(s) 221, 230, 233, 581
NaeI GCCGGC 1 cut(s) 231
NciI CCSGG 2 cut(s) 195, 816
NdeII GATC 2 cut(s) 307, 906
NgoMIV GCCGGC 1 cut(s) 229
NlaIII CATG 5 cut(s) 106, 160, 308, 358, 706
NlaIV GGNNCC 1 cut(s) 884
NmeAIII GCCGAG 1 cut(s) 68
NsiI ATGCAT 1 cut(s) 306
PdiI GCCGGC 1 cut(s) 231
PdmI GAANNNNTTC 1 cut(s) 717
PfeI GAWTC 2 cut(s) 873, 922
Pfl23II CGTACG 1 cut(s) 996
PflFI GACNNNGTC 1 cut(s) 476
PflMI CCANNNNNTGG 1 cut(s) 938
PkrI GCNGC 5 cut(s) 69, 226, 235, 400, 628
PleI GAGTC 3 cut(s) 76, 102, 292
PpsI GAGTC 3 cut(s) 76, 102, 292
Ppu21I YACGTR 2 cut(s) 996, 1000
PpuMI RGGWCCY 1 cut(s) 883
PshAI GACNNNNGTC 1 cut(s) 120
PsiI TTATAA 1 cut(s) 869
Psp5II RGGWCCY 1 cut(s) 883
Psp6I CCWGG 2 cut(s) 447, 576
PspGI CCWGG 2 cut(s) 447, 576
PspLI CGTACG 1 cut(s) 996
PspN4I GGNNCC 1 cut(s) 884
PspPI GGNCC 2 cut(s) 800, 883
PspPPI RGGWCCY 1 cut(s) 883
PsyI GACNNNGTC 1 cut(s) 476
RsaI GTAC 2 cut(s) 987, 998
RsaNI GTAC 2 cut(s) 986, 997
RseI CAYNNNNRTG 3 cut(s) 30, 158, 701
SaqAI TTAA 1 cut(s) 261
SatI GCNGC 5 cut(s) 68, 225, 234, 399, 627
Sau3AI GATC 2 cut(s) 307, 906
Sau96I GGNCC 2 cut(s) 800, 883
SchI GAGTC 3 cut(s) 76, 102, 293
ScrFI CCNGG 4 cut(s) 195, 449, 578, 816
SduI GDGCHC 1 cut(s) 695
SfaNI GCATC 1 cut(s) 150
SinI GGWCC 2 cut(s) 800, 883
SmiMI CAYNNNNRTG 3 cut(s) 30, 158, 701
SnaBI TACGTA 1 cut(s) 1000
Sse9I AATT 8 cut(s) 369, 381, 483, 532, 657, 807, 840, 898
SsiI CCGC 2 cut(s) 399, 770
SspI AATATT 2 cut(s) 190, 716
StyD4I CCNGG 4 cut(s) 193, 447, 576, 814
StyI CCWWGG 1 cut(s) 646
TaaI ACNGT 2 cut(s) 119, 688
TaiI ACGT 3 cut(s) 573, 998, 1002
TaqI TCGA 6 cut(s) 9, 48, 111, 211, 753, 805
TasI AATT 8 cut(s) 369, 381, 483, 532, 657, 807, 840, 898
TatI WGTACW 1 cut(s) 985
TauI GCSGC 1 cut(s) 401
TfiI GAWTC 2 cut(s) 873, 922
Tru1I TTAA 1 cut(s) 261
Tru9I TTAA 1 cut(s) 261
TscAI CASTG 2 cut(s) 32, 693
TseI GCWGC 4 cut(s) 67, 224, 233, 626
TspDTI ATGAA 8 cut(s) 91, 197, 233, 343, 381, 569, 719, 935
TspRI CASTG 2 cut(s) 32, 693
Tth111I GACNNNGTC 1 cut(s) 476
Van91I CCANNNNNTGG 1 cut(s) 938
VpaK11BI GGWCC 2 cut(s) 800, 883
XapI RAATTY 2 cut(s) 807, 840
XcmI CCANNNNNNNNNTGG 1 cut(s) 100
XmiI GTMKAC 2 cut(s) 287, 678
XmnI GAANNNNTTC 1 cut(s) 717
Zsp2I ATGCAT 1 cut(s) 306
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.