Rroxscaffold_2G00114580

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0000778
Physical Location & Seq
Forward (+)
40748845 .. 40752492
3648 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00114580.1

Sequence Viewer

Length: 1461 bp
ATGGATTACAGTAACCTTTATATTTTCTTGTCTACACTACTTGCCATCTTCACTACTACTACTCTTGCATTCCTTTTTTCTGCTTATAAGTCAAAATCCCATGCAAAATCCATTAATCTTCCCCCAGGCAGTTTTGGGTGGCCGATTATCGGCGAAACCTTTGCCTTCCTACACAATGACCATGAAAAATTTATAGGGGATAGAATGAAGAAATACTCCTCCAAAATCTTCAAAACCAAGATACTAGGTGAGCGAACAGTGGTGTTATGTGGCACTGCCGGTCACAAATTTGTGGCTTCCAACGAAGAAAAGCTCTTTGCAGCATGGAGACCTCACTCAATGCAAAAGCTCTTCCGTTCTTCATACCAAAAAGCTGCTTCCATCATCATGCCAAAACAAATGGAAAAGCACGTATCGCAGGCACCGGGGTTTCTGAGAGCCGAAGCCCTGGTTGGCTACGTTGCAGCGTTGGATTCCATGGTTCTCGACCACCTGAAAACGCACTGGGATGGCAAACAGGTGGTCGAGGCTCATCGCCTCACGCAGCTACTCGTTTTGACTCTCTCAGCCAAGTCATTCATGGGACTCGAAGACCATTGTCGTATTGATAAGCTTGCGAAGTTGATGGACACCATGATGCCGGCGCTTCACACTATTCCTGTGAATATTCCGGGGACTGCATTTCATCGAGCAATGAAAGCAGTGCCGGCTGCGAGGGAGATTATTCAGTCGTTTGTTAAGGAGAAGAAAGAAGTTATGGCGTCTACAGGAAACAAAATGCATGATTTTTTGTCTTATATGATTGCTACGGCAGACCCAGTTACCGGAAAGTTCATGCCGGATAATGAAATTGCTGACAAAATCATGGGGATTTTAGCCGCTTCGTTCAACTCTCCAGCTATGACTATTACTTTTATCGTGAAGTTCCTGGGCGAAAGACCTGACATTTATGACAAAGTCCGAATTGAACAAATGGAGATTTTGAAGTCCAAAAAATCTGACGAGGCACTCAATTGGGATGACTTGCAAAAAATGAAGTATTCGTGGAACGTAGCCTTGGAGGCGATGAGGCTCATACCACCTCTTCAGGGAACATTTAGAGAGGCTGCAAAGGACTTCACTTACGAAGGTTACACAATTCCTAAAGGATGGAAGGTCTACTGGACAGTGAGCACCACAACCATGAACCCTGAATATTTTCCAGCGCCAGAAGAGTTTGATCCTACAAGGTTCGAGAAAGCAACTCCTCCGCCTTACACAAACATCCCATTTGGGAGCGGTCCTCGAATTTGCCCCGGAAAGGACTACGCTCGTCTGCAAATTCTTAGTTTTATGCACCATCTTGTTATAAGATTCAAATGGGAACTTGTGAACCCTACTTGCAAGATCACAGGTGGTATGAATCCCTTTCCAGTTGATGGGCTGAAGGTTCGCCTCCAAGCTCAACCCAGTCAAGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
p450

Protein Analysis

486

Amino Acids

55.0

Weight (kDa)

9.29

Isoelectric Point (pI)

36.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 41 - 459 6.4e-57 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 87, 1349
AccB1I GGYRCC 1 cut(s) 421
AccB7I CCANNNNNTGG 1 cut(s) 1418
AccBSI CCGCTC 1 cut(s) 1278
AccI GTMKAC 3 cut(s) 32, 764, 1158
AciI CCGC 3 cut(s) 879, 1250, 1278
AclWI GGATC 1 cut(s) 1214
AcoI YGGCCR 1 cut(s) 140
AcsI RAATTY 4 cut(s) 188, 287, 1287, 1320
AcuI CTGAAG 2 cut(s) 1070, 1445
AcyI GRCGYC 1 cut(s) 761
AfiI CCNNNNNNNGG 5 cut(s) 149, 824, 1088, 1300, 1418
AgsI TTSAA 5 cut(s) 232, 889, 968, 985, 1357
AjnI CCWGG 3 cut(s) 124, 447, 927
AjuI GAANNNNNNNTTGG 4 cut(s) 435, 467, 1040, 1072
AluBI AGCT 8 cut(s) 313, 349, 374, 547, 613, 899, 1442, 1457
AluI AGCT 8 cut(s) 313, 349, 374, 547, 613, 899, 1442, 1457
Alw21I GWGCWC 1 cut(s) 1175
Alw26I GTCTC 1 cut(s) 322
AlwI GGATC 1 cut(s) 1214
AoxI GGCC 1 cut(s) 140
ApeKI GCWGC 6 cut(s) 320, 374, 464, 544, 710, 1106
ApoI RAATTY 4 cut(s) 188, 287, 1287, 1320
ArsI GACNNNNNNTTYG 2 cut(s) 507, 539
AseI ATTAAT 1 cut(s) 114
Asp700I GAANNNNTTC 1 cut(s) 1197
AspLEI GCGC 2 cut(s) 646, 1207
AspS9I GGNCC 1 cut(s) 1280
AsuC2I CCSGG 3 cut(s) 426, 672, 1296
AsuHPI GGTGA 1 cut(s) 260
AvaII GGWCC 1 cut(s) 1280
BanI GGYRCC 1 cut(s) 421
BbsI GAAGAC 1 cut(s) 597
Bbv12I GWGCWC 1 cut(s) 1175
BbvI GCAGC 6 cut(s) 332, 361, 476, 556, 697, 1093
BccI CCATC 7 cut(s) 53, 389, 503, 619, 1143, 1347, 1412
BceAI ACGGC 1 cut(s) 825
BcgI CGANNNNNNTGC 2 cut(s) 143, 177
BciT130I CCWGG 3 cut(s) 126, 449, 929
BcnI CCSGG 3 cut(s) 426, 672, 1296
BcoDI GTCTC 1 cut(s) 322
BfaI CTAG 1 cut(s) 245
BfmI CTRYAG 1 cut(s) 765
BfoI RGCGCY 2 cut(s) 647, 1208
BglI GCCNNNNNGGC 1 cut(s) 1061
BisI GCNGC 7 cut(s) 321, 375, 465, 545, 711, 879, 1107
BlsI GCNGC 7 cut(s) 322, 376, 466, 546, 712, 880, 1108
Bme1390I CCNGG 6 cut(s) 126, 426, 449, 672, 929, 1296
Bme18I GGWCC 1 cut(s) 1280
BmgT120I GGNCC 1 cut(s) 1280
BmiI GGNNCC 1 cut(s) 423
BmrFI CCNGG 6 cut(s) 126, 426, 449, 672, 929, 1296
BmrI ACTGGG 3 cut(s) 514, 812, 1443
BmsI GCATC 1 cut(s) 627
BmuI ACTGGG 3 cut(s) 514, 812, 1443
BoxI GACNNNNGTC 1 cut(s) 597
BpiI GAAGAC 1 cut(s) 597
BplI GAGNNNNNCTC 2 cut(s) 1267, 1299
BpmI CTGGAG 1 cut(s) 879
BpuMI CCSGG 3 cut(s) 426, 672, 1296
BsaAI YACGTR 1 cut(s) 412
BsaHI GRCGYC 1 cut(s) 761
BsaI GGTCTC 1 cut(s) 322
BsaJI CCNNGG 8 cut(s) 124, 425, 447, 477, 671, 928, 1056, 1294
BsaWI WCCGGW 1 cut(s) 824
Bsc4I CCNNNNNNNGG 5 cut(s) 149, 824, 1088, 1300, 1418
Bse118I RCCGGY 3 cut(s) 278, 640, 706
Bse1I ACTGG 5 cut(s) 509, 818, 1166, 1412, 1449
Bse3DI GCAATG 1 cut(s) 699
BseBI CCWGG 3 cut(s) 126, 449, 929
BseDI CCNNGG 8 cut(s) 124, 425, 447, 477, 671, 928, 1056, 1294
BseGI GGATG 4 cut(s) 514, 1024, 1154, 1263
BseLI CCNNNNNNNGG 5 cut(s) 149, 824, 1088, 1300, 1418
BseMI GCAATG 1 cut(s) 699
BseMII CTCAG 2 cut(s) 425, 579
BseNI ACTGG 5 cut(s) 509, 818, 1166, 1412, 1449
BseRI GAGGAG 2 cut(s) 208, 1236
BseXI GCAGC 6 cut(s) 332, 361, 476, 556, 697, 1093
BshFI GGCC 1 cut(s) 142
BshNI GGYRCC 1 cut(s) 421
BsiHKAI GWGCWC 1 cut(s) 1175
BsiSI CCGG 8 cut(s) 279, 425, 641, 671, 707, 825, 839, 1296
BslFI GGGAC 2 cut(s) 597, 688
BslI CCNNNNNNNGG 5 cut(s) 149, 824, 1088, 1300, 1418
BsmAI GTCTC 1 cut(s) 322
BsmFI GGGAC 2 cut(s) 597, 688
BsmI GAATGC 1 cut(s) 68
BsnI GGCC 1 cut(s) 142
Bso31I GGTCTC 1 cut(s) 322
Bsp1286I GDGCHC 1 cut(s) 1175
Bsp143I GATC 2 cut(s) 1219, 1386
Bsp19I CCATGG 1 cut(s) 477
BspACI CCGC 3 cut(s) 879, 1250, 1278
BspANI GGCC 1 cut(s) 142
BspCNI CTCAG 2 cut(s) 426, 578
BspLI GGNNCC 1 cut(s) 423
BspPI GGATC 1 cut(s) 1214
BspQI GCTCTTC 1 cut(s) 356
BspT107I GGYRCC 1 cut(s) 421
BspTNI GGTCTC 1 cut(s) 322
BsrBI CCGCTC 1 cut(s) 1278
BsrDI GCAATG 1 cut(s) 699
BsrFI RCCGGY 3 cut(s) 278, 640, 706
BsrI ACTGG 5 cut(s) 509, 818, 1166, 1412, 1449
BssAI RCCGGY 3 cut(s) 278, 640, 706
BssECI CCNNGG 8 cut(s) 124, 425, 447, 477, 671, 928, 1056, 1294
BssMI GATC 2 cut(s) 1219, 1386
BssNI GRCGYC 1 cut(s) 761
BssT1I CCWWGG 2 cut(s) 477, 1056
Bst2UI CCWGG 3 cut(s) 126, 449, 929
Bst4CI ACNGT 3 cut(s) 11, 259, 1168
Bst6I CTCTTC 3 cut(s) 356, 1089, 1206
BstACI GRCGYC 1 cut(s) 761
BstBAI YACGTR 1 cut(s) 412
BstC8I GCNNGC 4 cut(s) 420, 615, 642, 708
BstDEI CTNAG 3 cut(s) 434, 565, 1325
BstDSI CCRYGG 1 cut(s) 477
BstF5I GGATG 4 cut(s) 514, 1024, 1154, 1263
BstH2I RGCGCY 2 cut(s) 647, 1208
BstHHI GCGC 2 cut(s) 646, 1207
BstKTI GATC 2 cut(s) 1222, 1389
BstMAI GTCTC 1 cut(s) 322
BstMBI GATC 2 cut(s) 1219, 1386
BstMWI GCNNNNNNNGC 4 cut(s) 415, 698, 707, 1061
BstNI CCWGG 3 cut(s) 126, 449, 929
BstPAI GACNNNNGTC 1 cut(s) 597
BstSCI CCNGG 6 cut(s) 124, 424, 447, 670, 927, 1294
BstSFI CTRYAG 1 cut(s) 765
BstV1I GCAGC 6 cut(s) 332, 361, 476, 556, 697, 1093
BstV2I GAAGAC 1 cut(s) 597
BsuRI GGCC 1 cut(s) 142
BtgI CCRYGG 1 cut(s) 477
BtgZI GCGATG 2 cut(s) 518, 1079
BtsCI GGATG 4 cut(s) 514, 1024, 1154, 1263
BtsI GCAGTG 2 cut(s) 273, 708
BtsIMutI CAGTG 5 cut(s) 264, 273, 502, 708, 1173
Cac8I GCNNGC 4 cut(s) 420, 615, 642, 708
CfoI GCGC 2 cut(s) 646, 1207
Cfr10I RCCGGY 3 cut(s) 278, 640, 706
Cfr13I GGNCC 1 cut(s) 1280
CseI GACGC 1 cut(s) 750
DdeI CTNAG 3 cut(s) 434, 565, 1325
DpnI GATC 2 cut(s) 1221, 1388
DpnII GATC 2 cut(s) 1219, 1386
EaeI YGGCCR 1 cut(s) 140
Eam1104I CTCTTC 3 cut(s) 356, 1089, 1206
EarI CTCTTC 3 cut(s) 356, 1089, 1206
EciI GGCGGA 1 cut(s) 1239
Eco130I CCWWGG 2 cut(s) 477, 1056
Eco31I GGTCTC 1 cut(s) 322
Eco47I GGWCC 1 cut(s) 1280
Eco57I CTGAAG 2 cut(s) 1070, 1445
EcoRII CCWGG 3 cut(s) 124, 447, 927
EcoT14I CCWWGG 2 cut(s) 477, 1056
EcoT22I ATGCAT 1 cut(s) 783
ErhI CCWWGG 2 cut(s) 477, 1056
FaqI GGGAC 2 cut(s) 597, 688
FblI GTMKAC 3 cut(s) 32, 764, 1158
Fnu4HI GCNGC 7 cut(s) 321, 375, 465, 545, 711, 879, 1107
FokI GGATG 4 cut(s) 521, 1031, 1161, 1250
Fsp4HI GCNGC 7 cut(s) 321, 375, 465, 545, 711, 879, 1107
FspBI CTAG 1 cut(s) 245
GlaI GCGC 2 cut(s) 645, 1206
GluI GCNGC 7 cut(s) 321, 375, 465, 545, 711, 879, 1107
GsuI CTGGAG 1 cut(s) 879
HaeII RGCGCY 2 cut(s) 647, 1208
HaeIII GGCC 1 cut(s) 142
HapII CCGG 8 cut(s) 279, 425, 641, 671, 707, 825, 839, 1296
HgaI GACGC 1 cut(s) 750
HhaI GCGC 2 cut(s) 646, 1207
Hin1I GRCGYC 1 cut(s) 761
Hin6I GCGC 2 cut(s) 644, 1205
HinP1I GCGC 2 cut(s) 644, 1205
HindIII AAGCTT 2 cut(s) 611, 1455
HinfI GANTC 5 cut(s) 473, 559, 585, 1353, 1402
HpaII CCGG 8 cut(s) 279, 425, 641, 671, 707, 825, 839, 1296
HphI GGTGA 1 cut(s) 260
Hpy166II GTNNAC 4 cut(s) 33, 765, 1159, 1372
Hpy188I TCNGA 3 cut(s) 435, 962, 1000
Hpy188III TCNNGA 3 cut(s) 485, 919, 1234
Hpy8I GTNNAC 4 cut(s) 33, 765, 1159, 1372
HpyAV CCTTC 4 cut(s) 175, 1121, 1147, 1420
HpyCH4III ACNGT 3 cut(s) 11, 259, 1168
HpyCH4IV ACGT 3 cut(s) 411, 459, 1050
HpyF10VI GCNNNNNNNGC 4 cut(s) 415, 698, 707, 1061
HpyF3I CTNAG 3 cut(s) 434, 565, 1325
HpySE526I ACGT 3 cut(s) 411, 459, 1050
Hsp92I GRCGYC 1 cut(s) 761
HspAI GCGC 2 cut(s) 644, 1205
KroI GCCGGC 2 cut(s) 640, 706
KroNI GCCGGC 2 cut(s) 642, 708
Kzo9I GATC 2 cut(s) 1219, 1386
LguI GCTCTTC 1 cut(s) 356
LmnI GCTCC 1 cut(s) 1275
Lsp1109I GCAGC 6 cut(s) 332, 361, 476, 556, 697, 1093
LweI GCATC 1 cut(s) 627
MaeI CTAG 1 cut(s) 245
MaeII ACGT 3 cut(s) 411, 459, 1050
MaeIII GTNAC 4 cut(s) 11, 281, 820, 1130
MalI GATC 2 cut(s) 1221, 1388
MbiI CCGCTC 1 cut(s) 1278
MboI GATC 2 cut(s) 1219, 1386
MfeI CAATTG 1 cut(s) 1012
MhlI GDGCHC 1 cut(s) 1175
MluCI AATT 8 cut(s) 188, 287, 849, 963, 1012, 1137, 1287, 1320
MlyI GAGTC 2 cut(s) 553, 579
MmeI TCCRAC 2 cut(s) 324, 450
Mph1103I ATGCAT 1 cut(s) 783
MroNI GCCGGC 2 cut(s) 640, 706
MroXI GAANNNNTTC 1 cut(s) 1197
MseI TTAA 3 cut(s) 114, 738, 1459
MslI CAYNNNNRTG 4 cut(s) 386, 507, 635, 1181
MspI CCGG 8 cut(s) 279, 425, 641, 671, 707, 825, 839, 1296
MspR9I CCNGG 6 cut(s) 126, 426, 449, 672, 929, 1296
MunI CAATTG 1 cut(s) 1012
Mva1269I GAATGC 1 cut(s) 68
MvaI CCWGG 3 cut(s) 126, 449, 929
MwoI GCNNNNNNNGC 4 cut(s) 415, 698, 707, 1061
NaeI GCCGGC 2 cut(s) 642, 708
NciI CCSGG 3 cut(s) 426, 672, 1296
NcoI CCATGG 1 cut(s) 477
NdeII GATC 2 cut(s) 1219, 1386
NgoMIV GCCGGC 2 cut(s) 640, 706
NlaIV GGNNCC 1 cut(s) 423
NmuCI GTSAC 1 cut(s) 281
NsiI ATGCAT 1 cut(s) 783
PciSI GCTCTTC 1 cut(s) 356
PctI GAATGC 1 cut(s) 68
PdiI GCCGGC 2 cut(s) 642, 708
PdmI GAANNNNTTC 1 cut(s) 1197
PfeI GAWTC 3 cut(s) 473, 1353, 1402
PflFI GACNNNGTC 1 cut(s) 956
PflMI CCANNNNNTGG 1 cut(s) 1418
PkrI GCNGC 7 cut(s) 322, 376, 466, 546, 712, 880, 1108
PleI GAGTC 2 cut(s) 553, 579
PpsI GAGTC 2 cut(s) 553, 579
Ppu21I YACGTR 1 cut(s) 412
PshAI GACNNNNGTC 1 cut(s) 597
PshBI ATTAAT 1 cut(s) 114
PsiI TTATAA 2 cut(s) 87, 1349
Psp6I CCWGG 3 cut(s) 124, 447, 927
PspGI CCWGG 3 cut(s) 124, 447, 927
PspN4I GGNNCC 1 cut(s) 423
PspPI GGNCC 1 cut(s) 1280
PsyI GACNNNGTC 1 cut(s) 956
RseI CAYNNNNRTG 4 cut(s) 386, 507, 635, 1181
SapI GCTCTTC 1 cut(s) 356
SaqAI TTAA 3 cut(s) 114, 738, 1459
SatI GCNGC 7 cut(s) 321, 375, 465, 545, 711, 879, 1107
Sau3AI GATC 2 cut(s) 1219, 1386
Sau96I GGNCC 1 cut(s) 1280
SchI GAGTC 2 cut(s) 553, 579
ScrFI CCNGG 6 cut(s) 126, 426, 449, 672, 929, 1296
SduI GDGCHC 1 cut(s) 1175
SfaNI GCATC 1 cut(s) 627
SfcI CTRYAG 1 cut(s) 765
SinI GGWCC 1 cut(s) 1280
SmiMI CAYNNNNRTG 4 cut(s) 386, 507, 635, 1181
Sse9I AATT 8 cut(s) 188, 287, 849, 963, 1012, 1137, 1287, 1320
SsiI CCGC 3 cut(s) 879, 1250, 1278
SspI AATATT 2 cut(s) 667, 1196
SspMI CTAG 1 cut(s) 245
StyD4I CCNGG 6 cut(s) 124, 424, 447, 670, 927, 1294
StyI CCWWGG 2 cut(s) 477, 1056
TaaI ACNGT 3 cut(s) 11, 259, 1168
TaiI ACGT 3 cut(s) 414, 462, 1053
TaqI TCGA 6 cut(s) 486, 525, 588, 688, 1233, 1285
TasI AATT 8 cut(s) 188, 287, 849, 963, 1012, 1137, 1287, 1320
TauI GCSGC 1 cut(s) 881
TfiI GAWTC 3 cut(s) 473, 1353, 1402
Tru1I TTAA 3 cut(s) 114, 738, 1459
Tru9I TTAA 3 cut(s) 114, 738, 1459
TscAI CASTG 5 cut(s) 264, 280, 509, 708, 1173
TseFI GTSAC 1 cut(s) 281
TseI GCWGC 6 cut(s) 320, 374, 464, 544, 710, 1106
Tsp45I GTSAC 1 cut(s) 281
TspGWI ACGGA 1 cut(s) 344
TspRI CASTG 5 cut(s) 264, 280, 509, 708, 1173
Tth111I GACNNNGTC 1 cut(s) 956
Van91I CCANNNNNTGG 1 cut(s) 1418
VpaK11BI GGWCC 1 cut(s) 1280
VspI ATTAAT 1 cut(s) 114
XapI RAATTY 4 cut(s) 188, 287, 1287, 1320
XcmI CCANNNNNNNNNTGG 1 cut(s) 577
XmiI GTMKAC 3 cut(s) 32, 764, 1158
XmnI GAANNNNTTC 1 cut(s) 1197
XspI CTAG 1 cut(s) 245
Zsp2I ATGCAT 1 cut(s) 783
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.