Rh4CG052100

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0000778
Physical Location & Seq
Forward (+)
10331099 .. 10335003
3905 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG052100.1

Sequence Viewer

Length: 828 bp
ATGAAGAAATACTCCTCCAAAATCTTCAAAACCAAGATACTAGGTGAGCGAACCGTGGTGTTATGTGGCACTGCCGGTCACAAATTTGTGGCTTCCAACGAAGAAAAGCTCTTTGCAGCATGGAGACCTCACTCAATGCAAAAGCTCTTTCGTTCTTCATACCAAAAAGCTGCTTCCACCATCATGCCAAGACAAATGGAAAAGCATGTATCTCAGGCACCGGGGTTTCTGCGAGCCGAAGCCCTGGTTGGCTACGTGGCAACGATGGATTCCATGGTTCTCGACCACCTGAAAACGCACTGGGATGGCAAACAGGTGGTCGAGGCTCATCGCCTCACGCAGCTACTGGTTTTGACTCTCTCGGCCAAGTTCTTCATGGGACTCGAAGATAGTTGTCGTATTGGTAAGCTTGTCGAGTTGATGGACACCATGATGCTGGCGCTTCACACTATTCCTGTGAATATTCCGGGGACTGCATTTCATAGAGCAATGAAAGCAGCGCCGGCTGCGAGGGAGATTATTCAGTCGTTTGTTAAGGAGAAGAAAGAAGTTATGGAGTCTACCGGAACCAAAATGCATGATCTTTTGTCTTATATGATTACTACGCCAGACCCAACCGGAAGGTTCATGCCAGAGAATGAAATTGCTGATAAAATTATGGGGAATGTGGCCGCTTCGTTCAACTCTCCAGCTATGACAACTACTTTTATCGTGAAGTTCTTGGGCGAAAGACCTGACATATATGACAAAGTCCGAATTGGGGAAATGTCGAAAATCTTATTGAGGACCAATAACAAGTACAAAATCATCATTTTCTTGTATAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
p450

Protein Analysis

275

Amino Acids

31.11

Weight (kDa)

9.66

Isoelectric Point (pI)

34.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 2 - 252 2.3e-09 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 217
AccI GTMKAC 1 cut(s) 560
AciI CCGC 1 cut(s) 672
AcoI YGGCCR 2 cut(s) 363, 669
AcsI RAATTY 1 cut(s) 83
AfaI GTAC 1 cut(s) 800
AfiI CCNNNNNNNGG 1 cut(s) 760
AgsI TTSAA 2 cut(s) 28, 682
AjnI CCWGG 1 cut(s) 243
AjuI GAANNNNNNNTTGG 2 cut(s) 231, 263
AluBI AGCT 6 cut(s) 109, 145, 170, 343, 409, 692
AluI AGCT 6 cut(s) 109, 145, 170, 343, 409, 692
Alw26I GTCTC 1 cut(s) 118
AlwNI CAGNNNCTG 1 cut(s) 346
AoxI GGCC 2 cut(s) 363, 669
ApeKI GCWGC 5 cut(s) 116, 170, 340, 497, 506
ApoI RAATTY 1 cut(s) 83
ArsI GACNNNNNNTTYG 2 cut(s) 303, 335
AspLEI GCGC 2 cut(s) 442, 502
AspS9I GGNCC 1 cut(s) 786
AsuC2I CCSGG 2 cut(s) 222, 468
AsuHPI GGTGA 1 cut(s) 56
AvaII GGWCC 1 cut(s) 786
BanI GGYRCC 1 cut(s) 217
BbvI GCAGC 5 cut(s) 128, 157, 352, 493, 509
BccI CCATC 4 cut(s) 188, 259, 299, 415
BciT130I CCWGG 1 cut(s) 245
BcnI CCSGG 2 cut(s) 222, 468
BcoDI GTCTC 1 cut(s) 118
BfaI CTAG 1 cut(s) 41
BfoI RGCGCY 2 cut(s) 443, 503
BisI GCNGC 6 cut(s) 117, 171, 341, 498, 507, 672
BlsI GCNGC 6 cut(s) 118, 172, 342, 499, 508, 673
Bme1390I CCNGG 3 cut(s) 222, 245, 468
Bme18I GGWCC 1 cut(s) 786
BmgT120I GGNCC 1 cut(s) 786
BmiI GGNNCC 2 cut(s) 219, 568
BmrFI CCNGG 3 cut(s) 222, 245, 468
BmrI ACTGGG 1 cut(s) 310
BmsI GCATC 1 cut(s) 423
BmuI ACTGGG 1 cut(s) 310
BpmI CTGGAG 1 cut(s) 672
BpuMI CCSGG 2 cut(s) 222, 468
BsaAI YACGTR 1 cut(s) 256
BsaI GGTCTC 1 cut(s) 118
BsaJI CCNNGG 5 cut(s) 54, 221, 243, 273, 467
BsaWI WCCGGW 2 cut(s) 563, 617
Bsc4I CCNNNNNNNGG 1 cut(s) 760
Bse118I RCCGGY 2 cut(s) 74, 502
Bse1I ACTGG 2 cut(s) 305, 351
Bse3DI GCAATG 1 cut(s) 495
BseBI CCWGG 1 cut(s) 245
BseDI CCNNGG 5 cut(s) 54, 221, 243, 273, 467
BseGI GGATG 1 cut(s) 310
BseLI CCNNNNNNNGG 1 cut(s) 760
BseMI GCAATG 1 cut(s) 495
BseMII CTCAG 1 cut(s) 227
BseNI ACTGG 2 cut(s) 305, 351
BseRI GAGGAG 1 cut(s) 4
BseXI GCAGC 5 cut(s) 128, 157, 352, 493, 509
BshFI GGCC 2 cut(s) 365, 671
BshNI GGYRCC 1 cut(s) 217
BsiSI CCGG 6 cut(s) 75, 221, 467, 503, 564, 618
BslFI GGGAC 2 cut(s) 393, 484
BslI CCNNNNNNNGG 1 cut(s) 760
BsmAI GTCTC 1 cut(s) 118
BsmFI GGGAC 2 cut(s) 393, 484
BsnI GGCC 2 cut(s) 365, 671
Bso31I GGTCTC 1 cut(s) 118
Bsp143I GATC 1 cut(s) 580
Bsp19I CCATGG 1 cut(s) 273
BspACI CCGC 1 cut(s) 672
BspANI GGCC 2 cut(s) 365, 671
BspCNI CTCAG 1 cut(s) 226
BspLI GGNNCC 2 cut(s) 219, 568
BspT107I GGYRCC 1 cut(s) 217
BspTNI GGTCTC 1 cut(s) 118
BsrDI GCAATG 1 cut(s) 495
BsrFI RCCGGY 2 cut(s) 74, 502
BsrI ACTGG 2 cut(s) 305, 351
BssAI RCCGGY 2 cut(s) 74, 502
BssECI CCNNGG 5 cut(s) 54, 221, 243, 273, 467
BssMI GATC 1 cut(s) 580
BssT1I CCWWGG 1 cut(s) 273
Bst2UI CCWGG 1 cut(s) 245
Bst4CI ACNGT 1 cut(s) 55
BstBAI YACGTR 1 cut(s) 256
BstC8I GCNNGC 3 cut(s) 234, 438, 504
BstDEI CTNAG 1 cut(s) 213
BstDSI CCRYGG 2 cut(s) 54, 273
BstF5I GGATG 1 cut(s) 310
BstH2I RGCGCY 2 cut(s) 443, 503
BstHHI GCGC 2 cut(s) 442, 502
BstKTI GATC 1 cut(s) 583
BstMAI GTCTC 1 cut(s) 118
BstMBI GATC 1 cut(s) 580
BstMWI GCNNNNNNNGC 3 cut(s) 494, 503, 506
BstNI CCWGG 1 cut(s) 245
BstNSI RCATGY 1 cut(s) 209
BstSCI CCNGG 3 cut(s) 220, 243, 466
BstV1I GCAGC 5 cut(s) 128, 157, 352, 493, 509
BstXI CCANNNNNNTGG 1 cut(s) 436
BsuRI GGCC 2 cut(s) 365, 671
BtgI CCRYGG 2 cut(s) 54, 273
BtgZI GCGATG 1 cut(s) 314
BtsCI GGATG 1 cut(s) 310
BtsI GCAGTG 1 cut(s) 69
BtsIMutI CAGTG 2 cut(s) 69, 298
Cac8I GCNNGC 3 cut(s) 234, 438, 504
CaiI CAGNNNCTG 1 cut(s) 346
CfoI GCGC 2 cut(s) 442, 502
Cfr10I RCCGGY 2 cut(s) 74, 502
Cfr13I GGNCC 1 cut(s) 786
Csp6I GTAC 1 cut(s) 799
CviAII CATG 8 cut(s) 120, 184, 206, 274, 376, 430, 578, 628
CviQI GTAC 1 cut(s) 799
DdeI CTNAG 1 cut(s) 213
DpnI GATC 1 cut(s) 582
DpnII GATC 1 cut(s) 580
EaeI YGGCCR 2 cut(s) 363, 669
Eco130I CCWWGG 1 cut(s) 273
Eco31I GGTCTC 1 cut(s) 118
Eco47I GGWCC 1 cut(s) 786
EcoRII CCWGG 1 cut(s) 243
EcoT14I CCWWGG 1 cut(s) 273
EcoT22I ATGCAT 1 cut(s) 579
ErhI CCWWGG 1 cut(s) 273
FaeI CATG 8 cut(s) 123, 187, 209, 277, 379, 433, 581, 631
FaqI GGGAC 2 cut(s) 393, 484
FatI CATG 8 cut(s) 119, 183, 205, 273, 375, 429, 577, 627
FblI GTMKAC 1 cut(s) 560
Fnu4HI GCNGC 6 cut(s) 117, 171, 341, 498, 507, 672
FokI GGATG 1 cut(s) 317
Fsp4HI GCNGC 6 cut(s) 117, 171, 341, 498, 507, 672
FspBI CTAG 1 cut(s) 41
GlaI GCGC 2 cut(s) 441, 501
GluI GCNGC 6 cut(s) 117, 171, 341, 498, 507, 672
GsuI CTGGAG 1 cut(s) 672
HaeII RGCGCY 2 cut(s) 443, 503
HaeIII GGCC 2 cut(s) 365, 671
HapII CCGG 6 cut(s) 75, 221, 467, 503, 564, 618
HhaI GCGC 2 cut(s) 442, 502
Hin1II CATG 8 cut(s) 123, 187, 209, 277, 379, 433, 581, 631
Hin6I GCGC 2 cut(s) 440, 500
HinP1I GCGC 2 cut(s) 440, 500
HindIII AAGCTT 1 cut(s) 407
HinfI GANTC 4 cut(s) 269, 355, 381, 557
HpaII CCGG 6 cut(s) 75, 221, 467, 503, 564, 618
HphI GGTGA 1 cut(s) 56
Hpy166II GTNNAC 1 cut(s) 561
Hpy188I TCNGA 1 cut(s) 755
Hpy188III TCNNGA 2 cut(s) 281, 712
Hpy8I GTNNAC 1 cut(s) 561
HpyAV CCTTC 1 cut(s) 615
HpyCH4III ACNGT 1 cut(s) 55
HpyCH4IV ACGT 1 cut(s) 255
HpyCH4V TGCA 4 cut(s) 116, 139, 476, 577
HpyF10VI GCNNNNNNNGC 3 cut(s) 494, 503, 506
HpyF3I CTNAG 1 cut(s) 213
HpySE526I ACGT 1 cut(s) 255
Hsp92II CATG 8 cut(s) 123, 187, 209, 277, 379, 433, 581, 631
HspAI GCGC 2 cut(s) 440, 500
KroI GCCGGC 1 cut(s) 502
KroNI GCCGGC 1 cut(s) 504
Kzo9I GATC 1 cut(s) 580
Lsp1109I GCAGC 5 cut(s) 128, 157, 352, 493, 509
LweI GCATC 1 cut(s) 423
MaeI CTAG 1 cut(s) 41
MaeII ACGT 1 cut(s) 255
MaeIII GTNAC 1 cut(s) 77
MalI GATC 1 cut(s) 582
MboI GATC 1 cut(s) 580
MboII GAAGA 7 cut(s) 16, 16, 113, 147, 364, 398, 553
MluCI AATT 4 cut(s) 83, 642, 654, 756
MlyI GAGTC 3 cut(s) 349, 375, 566
MmeI TCCRAC 1 cut(s) 120
MnlI CCTC 6 cut(s) 25, 138, 316, 344, 504, 777
Mph1103I ATGCAT 1 cut(s) 579
MroNI GCCGGC 1 cut(s) 502
MseI TTAA 1 cut(s) 534
MslI CAYNNNNRTG 3 cut(s) 182, 303, 431
MspI CCGG 6 cut(s) 75, 221, 467, 503, 564, 618
MspR9I CCNGG 3 cut(s) 222, 245, 468
MvaI CCWGG 1 cut(s) 245
MwoI GCNNNNNNNGC 3 cut(s) 494, 503, 506
NaeI GCCGGC 1 cut(s) 504
NciI CCSGG 2 cut(s) 222, 468
NcoI CCATGG 1 cut(s) 273
NdeII GATC 1 cut(s) 580
NgoMIV GCCGGC 1 cut(s) 502
NlaIII CATG 8 cut(s) 123, 187, 209, 277, 379, 433, 581, 631
NlaIV GGNNCC 2 cut(s) 219, 568
NmeAIII GCCGAG 1 cut(s) 341
NmuCI GTSAC 1 cut(s) 77
NsiI ATGCAT 1 cut(s) 579
NspI RCATGY 1 cut(s) 209
PdiI GCCGGC 1 cut(s) 504
PfeI GAWTC 1 cut(s) 269
PflFI GACNNNGTC 1 cut(s) 749
PkrI GCNGC 6 cut(s) 118, 172, 342, 499, 508, 673
PleI GAGTC 3 cut(s) 349, 375, 565
PpsI GAGTC 3 cut(s) 349, 375, 565
Ppu21I YACGTR 1 cut(s) 256
Psp6I CCWGG 1 cut(s) 243
PspGI CCWGG 1 cut(s) 243
PspN4I GGNNCC 2 cut(s) 219, 568
PspPI GGNCC 1 cut(s) 786
PstNI CAGNNNCTG 1 cut(s) 346
PsyI GACNNNGTC 1 cut(s) 749
RsaI GTAC 1 cut(s) 800
RsaNI GTAC 1 cut(s) 799
RseI CAYNNNNRTG 3 cut(s) 182, 303, 431
SaqAI TTAA 1 cut(s) 534
SatI GCNGC 6 cut(s) 117, 171, 341, 498, 507, 672
Sau3AI GATC 1 cut(s) 580
Sau96I GGNCC 1 cut(s) 786
SchI GAGTC 3 cut(s) 349, 375, 566
ScrFI CCNGG 3 cut(s) 222, 245, 468
SfaNI GCATC 1 cut(s) 423
SinI GGWCC 1 cut(s) 786
SmiMI CAYNNNNRTG 3 cut(s) 182, 303, 431
Sse9I AATT 4 cut(s) 83, 642, 654, 756
SsiI CCGC 1 cut(s) 672
SspI AATATT 1 cut(s) 463
SspMI CTAG 1 cut(s) 41
StyD4I CCNGG 3 cut(s) 220, 243, 466
StyI CCWWGG 1 cut(s) 273
TaaI ACNGT 1 cut(s) 55
TaiI ACGT 1 cut(s) 258
TaqI TCGA 5 cut(s) 282, 321, 384, 414, 770
TasI AATT 4 cut(s) 83, 642, 654, 756
TatI WGTACW 1 cut(s) 798
TauI GCSGC 1 cut(s) 674
TfiI GAWTC 1 cut(s) 269
Tru1I TTAA 1 cut(s) 534
Tru9I TTAA 1 cut(s) 534
TscAI CASTG 2 cut(s) 76, 305
TseFI GTSAC 1 cut(s) 77
TseI GCWGC 5 cut(s) 116, 170, 340, 497, 506
Tsp45I GTSAC 1 cut(s) 77
TspDTI ATGAA 7 cut(s) 17, 147, 364, 470, 506, 616, 654
TspRI CASTG 2 cut(s) 76, 305
Tth111I GACNNNGTC 1 cut(s) 749
VpaK11BI GGWCC 1 cut(s) 786
XapI RAATTY 1 cut(s) 83
XceI RCATGY 1 cut(s) 209
XcmI CCANNNNNNNNNTGG 1 cut(s) 373
XmiI GTMKAC 1 cut(s) 560
XspI CTAG 1 cut(s) 41
Zsp2I ATGCAT 1 cut(s) 579
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.