RLG00000006727

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0000778
Physical Location & Seq
Forward (+)
9958412 .. 9960734
2323 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000006727

Sequence Viewer

Length: 831 bp
ATGGATTCCATGGTTCTCGACCCCCTGAAAACACACTGGGATGGCAAACAGGTGGTCGAGGCTCATCGCCTCACGCAGCTACTCGTTTTGACTCTCTCGGCTAAGTTCTTCATGGGACTCGAAGACAGTTATCGTATTGATAAGCTTGCGGAGTTGATTGACACCATGATGCTGGCGCTTCACACTGTTCGTGTGAATATTCCGGGAACTGCATTTTATCAAGCAATAAAAGCAGCGCAGGCTGTGAGGGAGACTATTCAGTCGTTTATTAAGGAGAAGAAGGAAGTTATGGCGTCTACCGGAAACAAAATGCATGATCTTTTGTCTTATTTGATTGCTACGGCAGACCCGGTTACCGGAAGGTTCATGCCGGAGAATGAAATTGCTGATAAAATTATGGGGAATGTGGCCGCTTCGTTCAACTCTCCAGCTATGACTACTACTTTTATCGTGAAGTACCTGGGCGAAAGACCTGACATTTATGACAAAGTTCGAATTGAACAACTGGAGATTTTGAAGTCCAAAAAATCTGGCGAGGCACTCAATTGGGATGATATGCAAAAAATGAAGTATTCGTGGAACGTAGCCCTGGAGGCAATGAGGCTCATACCACCTCTTCAGGGAACATTTAGAGAGGCTGCAACAGACTTCAGTTACGAAGGTTTTACAATTCCTAAAGGATGGAAGGTATATTGGACAGTGAGCACCACAAACATGAACCCTGAATGTTTTCCAGCGCCAGGAGAGCTTGATCCTACAAGGTTCGAGAAAGCAACTCCTCCGCCTTGCAAAAACATTCCATTTGGGAGCGCTCCTCGAATCTGCCCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
p450

Protein Analysis

277

Amino Acids

31.14

Weight (kDa)

6.46

Isoelectric Point (pI)

32.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 64 - 276 2.3e-35 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 259
AccI GTMKAC 1 cut(s) 296
AciI CCGC 3 cut(s) 149, 411, 782
AclWI GGATC 1 cut(s) 746
AcoI YGGCCR 1 cut(s) 408
AcuI CTGAAG 2 cut(s) 602, 634
AcyI GRCGYC 1 cut(s) 293
AfaI GTAC 1 cut(s) 458
AfeI AGCGCT 1 cut(s) 811
AfiI CCNNNNNNNGG 3 cut(s) 356, 620, 740
AgsI TTSAA 3 cut(s) 421, 500, 517
AjnI CCWGG 3 cut(s) 459, 588, 739
AluBI AGCT 4 cut(s) 79, 145, 431, 748
AluI AGCT 4 cut(s) 79, 145, 431, 748
Alw21I GWGCWC 1 cut(s) 707
Alw26I GTCTC 1 cut(s) 245
AlwI GGATC 1 cut(s) 746
Aor51HI AGCGCT 1 cut(s) 811
AoxI GGCC 1 cut(s) 408
ApeKI GCWGC 3 cut(s) 76, 233, 638
ArsI GACNNNNNNTTYG 2 cut(s) 39, 71
Asp700I GAANNNNTTC 1 cut(s) 729
AspLEI GCGC 4 cut(s) 178, 238, 739, 812
AsuC2I CCSGG 2 cut(s) 204, 350
AsuII TTCGAA 1 cut(s) 493
BbsI GAAGAC 1 cut(s) 129
Bbv12I GWGCWC 1 cut(s) 707
BbvI GCAGC 3 cut(s) 88, 245, 625
BccI CCATC 2 cut(s) 35, 675
BceAI ACGGC 1 cut(s) 357
BciT130I CCWGG 3 cut(s) 461, 590, 741
BcnI CCSGG 2 cut(s) 204, 350
BcoDI GTCTC 1 cut(s) 245
BfoI RGCGCY 3 cut(s) 179, 740, 813
BglI GCCNNNNNGGC 1 cut(s) 593
BisI GCNGC 4 cut(s) 77, 234, 411, 639
BlsI GCNGC 4 cut(s) 78, 235, 412, 640
Bme1390I CCNGG 5 cut(s) 204, 350, 461, 590, 741
BmrFI CCNGG 5 cut(s) 204, 350, 461, 590, 741
BmrI ACTGGG 1 cut(s) 46
BmsI GCATC 1 cut(s) 159
BmuI ACTGGG 1 cut(s) 46
BpiI GAAGAC 1 cut(s) 129
BplI GAGNNNNNCTC 2 cut(s) 799, 831
BpmI CTGGAG 3 cut(s) 411, 527, 611
Bpu14I TTCGAA 1 cut(s) 493
BpuMI CCSGG 2 cut(s) 204, 350
BsaHI GRCGYC 1 cut(s) 293
BsaJI CCNNGG 3 cut(s) 9, 460, 588
BsaWI WCCGGW 2 cut(s) 299, 356
Bsc4I CCNNNNNNNGG 3 cut(s) 356, 620, 740
Bse1I ACTGG 2 cut(s) 41, 510
Bse3DI GCAATG 1 cut(s) 603
BseBI CCWGG 3 cut(s) 461, 590, 741
BseDI CCNNGG 3 cut(s) 9, 460, 588
BseGI GGATG 3 cut(s) 46, 556, 686
BseLI CCNNNNNNNGG 3 cut(s) 356, 620, 740
BseMI GCAATG 1 cut(s) 603
BseNI ACTGG 2 cut(s) 41, 510
BseRI GAGGAG 2 cut(s) 768, 804
BseXI GCAGC 3 cut(s) 88, 245, 625
BshFI GGCC 1 cut(s) 410
BsiHKAI GWGCWC 1 cut(s) 707
BsiSI CCGG 5 cut(s) 203, 300, 350, 357, 371
BslFI GGGAC 1 cut(s) 129
BslI CCNNNNNNNGG 3 cut(s) 356, 620, 740
BsmAI GTCTC 1 cut(s) 245
BsmFI GGGAC 1 cut(s) 129
BsnI GGCC 1 cut(s) 410
Bsp119I TTCGAA 1 cut(s) 493
Bsp1286I GDGCHC 1 cut(s) 707
Bsp143I GATC 2 cut(s) 316, 751
Bsp19I CCATGG 1 cut(s) 9
BspACI CCGC 3 cut(s) 149, 411, 782
BspANI GGCC 1 cut(s) 410
BspPI GGATC 1 cut(s) 746
BspT104I TTCGAA 1 cut(s) 493
BsrDI GCAATG 1 cut(s) 603
BsrI ACTGG 2 cut(s) 41, 510
BssECI CCNNGG 3 cut(s) 9, 460, 588
BssMI GATC 2 cut(s) 316, 751
BssNI GRCGYC 1 cut(s) 293
BssT1I CCWWGG 1 cut(s) 9
Bst2UI CCWGG 3 cut(s) 461, 590, 741
Bst4CI ACNGT 3 cut(s) 128, 187, 700
Bst6I CTCTTC 1 cut(s) 621
BstACI GRCGYC 1 cut(s) 293
BstBI TTCGAA 1 cut(s) 493
BstC8I GCNNGC 3 cut(s) 147, 174, 240
BstDEI CTNAG 1 cut(s) 102
BstDSI CCRYGG 1 cut(s) 9
BstEII GGTNACC 1 cut(s) 352
BstF5I GGATG 3 cut(s) 46, 556, 686
BstH2I RGCGCY 3 cut(s) 179, 740, 813
BstHHI GCGC 4 cut(s) 178, 238, 739, 812
BstKTI GATC 2 cut(s) 319, 754
BstMAI GTCTC 1 cut(s) 245
BstMBI GATC 2 cut(s) 316, 751
BstMWI GCNNNNNNNGC 4 cut(s) 230, 239, 593, 745
BstNI CCWGG 3 cut(s) 461, 590, 741
BstPI GGTNACC 1 cut(s) 352
BstSCI CCNGG 5 cut(s) 202, 348, 459, 588, 739
BstV1I GCAGC 3 cut(s) 88, 245, 625
BstV2I GAAGAC 1 cut(s) 129
BstXI CCANNNNNNTGG 1 cut(s) 172
BsuRI GGCC 1 cut(s) 410
BtgI CCRYGG 1 cut(s) 9
BtgZI GCGATG 1 cut(s) 50
BtsCI GGATG 3 cut(s) 46, 556, 686
BtsIMutI CAGTG 3 cut(s) 34, 183, 705
Cac8I GCNNGC 3 cut(s) 147, 174, 240
CfoI GCGC 4 cut(s) 178, 238, 739, 812
CseI GACGC 1 cut(s) 282
Csp6I GTAC 1 cut(s) 457
CviAII CATG 6 cut(s) 10, 112, 166, 314, 367, 715
CviQI GTAC 1 cut(s) 457
DdeI CTNAG 1 cut(s) 102
DpnI GATC 2 cut(s) 318, 753
DpnII GATC 2 cut(s) 316, 751
DrdI GACNNNNNNGTC 1 cut(s) 259
DseDI GACNNNNNNGTC 1 cut(s) 259
EaeI YGGCCR 1 cut(s) 408
Eam1104I CTCTTC 1 cut(s) 621
EarI CTCTTC 1 cut(s) 621
EciI GGCGGA 1 cut(s) 771
Eco130I CCWWGG 1 cut(s) 9
Eco47III AGCGCT 1 cut(s) 811
Eco57I CTGAAG 2 cut(s) 602, 634
Eco91I GGTNACC 1 cut(s) 352
EcoO65I GGTNACC 1 cut(s) 352
EcoRII CCWGG 3 cut(s) 459, 588, 739
EcoT14I CCWWGG 1 cut(s) 9
EcoT22I ATGCAT 1 cut(s) 315
ErhI CCWWGG 1 cut(s) 9
FaeI CATG 6 cut(s) 13, 115, 169, 317, 370, 718
FaqI GGGAC 1 cut(s) 129
FatI CATG 6 cut(s) 9, 111, 165, 313, 366, 714
FblI GTMKAC 1 cut(s) 296
Fnu4HI GCNGC 4 cut(s) 77, 234, 411, 639
FokI GGATG 3 cut(s) 53, 563, 693
Fsp4HI GCNGC 4 cut(s) 77, 234, 411, 639
GlaI GCGC 4 cut(s) 177, 237, 738, 811
GluI GCNGC 4 cut(s) 77, 234, 411, 639
GsuI CTGGAG 3 cut(s) 411, 527, 611
HaeII RGCGCY 3 cut(s) 179, 740, 813
HaeIII GGCC 1 cut(s) 410
HapII CCGG 5 cut(s) 203, 300, 350, 357, 371
HgaI GACGC 1 cut(s) 282
HhaI GCGC 4 cut(s) 178, 238, 739, 812
Hin1I GRCGYC 1 cut(s) 293
Hin1II CATG 6 cut(s) 13, 115, 169, 317, 370, 718
Hin6I GCGC 4 cut(s) 176, 236, 737, 810
HinP1I GCGC 4 cut(s) 176, 236, 737, 810
HindIII AAGCTT 1 cut(s) 143
HinfI GANTC 4 cut(s) 5, 91, 117, 819
HpaII CCGG 5 cut(s) 203, 300, 350, 357, 371
Hpy166II GTNNAC 1 cut(s) 297
Hpy188III TCNNGA 3 cut(s) 17, 451, 766
Hpy8I GTNNAC 1 cut(s) 297
HpyAV CCTTC 4 cut(s) 274, 354, 653, 679
HpyCH4III ACNGT 3 cut(s) 128, 187, 700
HpyCH4IV ACGT 1 cut(s) 582
HpyCH4V TGCA 5 cut(s) 212, 313, 559, 641, 789
HpyF10VI GCNNNNNNNGC 4 cut(s) 230, 239, 593, 745
HpyF3I CTNAG 1 cut(s) 102
HpySE526I ACGT 1 cut(s) 582
Hsp92I GRCGYC 1 cut(s) 293
Hsp92II CATG 6 cut(s) 13, 115, 169, 317, 370, 718
HspAI GCGC 4 cut(s) 176, 236, 737, 810
Kzo9I GATC 2 cut(s) 316, 751
LmnI GCTCC 2 cut(s) 807, 817
Lsp1109I GCAGC 3 cut(s) 88, 245, 625
LweI GCATC 1 cut(s) 159
MaeII ACGT 1 cut(s) 582
MaeIII GTNAC 2 cut(s) 352, 653
MalI GATC 2 cut(s) 318, 753
MboI GATC 2 cut(s) 316, 751
MboII GAAGA 4 cut(s) 100, 134, 289, 608
MfeI CAATTG 1 cut(s) 544
MhlI GDGCHC 1 cut(s) 707
MluCI AATT 5 cut(s) 381, 393, 495, 544, 669
MlyI GAGTC 2 cut(s) 85, 111
Mph1103I ATGCAT 1 cut(s) 315
MroXI GAANNNNTTC 1 cut(s) 729
MseI TTAA 1 cut(s) 270
MslI CAYNNNNRTG 3 cut(s) 39, 167, 713
MspI CCGG 5 cut(s) 203, 300, 350, 357, 371
MspR9I CCNGG 5 cut(s) 204, 350, 461, 590, 741
MunI CAATTG 1 cut(s) 544
MvaI CCWGG 3 cut(s) 461, 590, 741
MwoI GCNNNNNNNGC 4 cut(s) 230, 239, 593, 745
NciI CCSGG 2 cut(s) 204, 350
NcoI CCATGG 1 cut(s) 9
NdeII GATC 2 cut(s) 316, 751
NlaIII CATG 6 cut(s) 13, 115, 169, 317, 370, 718
NmeAIII GCCGAG 1 cut(s) 77
NsiI ATGCAT 1 cut(s) 315
NspV TTCGAA 1 cut(s) 493
PdmI GAANNNNTTC 1 cut(s) 729
PfeI GAWTC 2 cut(s) 5, 819
PfoI TCCNGGA 1 cut(s) 202
PkrI GCNGC 4 cut(s) 78, 235, 412, 640
PleI GAGTC 2 cut(s) 85, 111
PpsI GAGTC 2 cut(s) 85, 111
Psp6I CCWGG 3 cut(s) 459, 588, 739
PspEI GGTNACC 1 cut(s) 352
PspGI CCWGG 3 cut(s) 459, 588, 739
RsaI GTAC 1 cut(s) 458
RsaNI GTAC 1 cut(s) 457
RseI CAYNNNNRTG 3 cut(s) 39, 167, 713
SaqAI TTAA 1 cut(s) 270
SatI GCNGC 4 cut(s) 77, 234, 411, 639
Sau3AI GATC 2 cut(s) 316, 751
SchI GAGTC 2 cut(s) 85, 111
ScrFI CCNGG 5 cut(s) 204, 350, 461, 590, 741
SduI GDGCHC 1 cut(s) 707
SfaNI GCATC 1 cut(s) 159
SfuI TTCGAA 1 cut(s) 493
SmiMI CAYNNNNRTG 3 cut(s) 39, 167, 713
Sse9I AATT 5 cut(s) 381, 393, 495, 544, 669
SsiI CCGC 3 cut(s) 149, 411, 782
SspI AATATT 1 cut(s) 199
StyD4I CCNGG 5 cut(s) 202, 348, 459, 588, 739
StyI CCWWGG 1 cut(s) 9
TaaI ACNGT 3 cut(s) 128, 187, 700
TaiI ACGT 1 cut(s) 585
TaqI TCGA 6 cut(s) 18, 57, 120, 493, 765, 817
TasI AATT 5 cut(s) 381, 393, 495, 544, 669
TauI GCSGC 1 cut(s) 413
TfiI GAWTC 2 cut(s) 5, 819
Tru1I TTAA 1 cut(s) 270
Tru9I TTAA 1 cut(s) 270
TscAI CASTG 3 cut(s) 41, 190, 705
TseI GCWGC 3 cut(s) 76, 233, 638
TspDTI ATGAA 5 cut(s) 100, 355, 393, 581, 731
TspRI CASTG 3 cut(s) 41, 190, 705
XmiI GTMKAC 1 cut(s) 296
XmnI GAANNNNTTC 1 cut(s) 729
Zsp2I ATGCAT 1 cut(s) 315
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.