Rh4AG048800

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0000778
Physical Location & Seq
Forward (+)
10252053 .. 10254576
2524 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG048800.1

Sequence Viewer

Length: 1458 bp
ATGGATTACAGTAACCTTTACATTTTCTTGTCTACACTACTTGCCATCTTCACTACTACTACTCTTGCATTCCTTTTTTCTGCTTCCAAGTCAAAATCCCATGAAAAATCCATTAATCTTCCCCCAGGCAGCTTTGGGTGGCCGATTATCGGCGAAACCTTTGCCTTCCTACACGATGACCATGAAAAATTTGTAGGGGATAGGATGAAGAAATACTCCTCCAAAATCTTCAAAACCAAGATACTAGGTGAGCGAACCGTGGTGTTATGTGGCACTGCCGGTCACAAATTTGTGGCTTCCAACGAAGAAAAGCTCTTTGCAGCATGGAGACCTCACTCAATGCAAAAGCTCTTTCGTTCTTCATACCAAAAAGCTGCTTCCACCATCATGCCAAGACAAATGGAAAAGCATGTATCTCAGGCACCGGGGTTTCTGCGAGCCGAAGCCCTGGTTGGCTACGTGGCAACGATGGATTCCATGGTTCTCGACCACCTGAAAACGCACTGGGATGGCAAACAGGTGGTCGAGGCTCATCGCCTCACGCAGCTACTGGTTTTGACTCTCTCGGCCAAGTTCTTCATGGGACTCGAAGATAGTTGTCGTATTGGTAAGCTTGTCGAGTTGATGGACACCATGATGCTGGCGCTTCACACTATTCCTGTGAATATTCCGGGGACTGCATTTCATAGAGCAATGAAAGCAGCGCCGGCTGCGAGGGAGATTATTCAGTCGTTTGTTAAGGAGAAGAAAGAAGTTATGGAGTCTACCGGAACCAAAATGCATGATCTTTTGTCTTATATGATTACTACGCCAGACCCAACCGGAAGGTTCATGCCAGAGAATGAAATTGCTGATAAAATTATGGGGAATGTGGCCGCTTCGTTCAACTCTCCAGCTATGACAACTACTTTTATCGTGAAGTTCTTGGGCGAAAGACCTGACATATATGACAAAGTCCGAATTGAGCAACTGGAGATTCTGAAGTCCAAAAAATCTGGCGAGACACTCAATTGGGATGATATGCAAAAAATGAAGTATTCATGGAACGTAGCCTTGGAGGCGATGAGGCTCATACCACCTCTTCAGGGAACATTTAGAGAGGCTGCAACAGACTTCACTTACCAAGGTTATACAATTCCTAAAGGATGGAAGGTCTACTGGACAGTGAGCACCACAACCATGAACCCTGAATATTTTCCTGCGCCAGAAGAGTTTGACCCTTCAAGGTTCGAGAAAGCAACTCCTCCGCCTTACACAAACATCCCATTCGGGAGTGGTCCTCGAATTTGCCCCGGAAAGGACTACGCTCGTCTGCAAATTCTTAGTTTTATGCACCATCTTGTTATAAGATTCAAATGGGAACTTGTGAACCATAATTGCAAGATCACAGGTGGTATGAATCCCTTTCCAGTTGATGGGCTGAAGGTTCGTCTCCAAGCTCAACCCAATCAAGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
p450

Protein Analysis

485

Amino Acids

55.0

Weight (kDa)

9.16

Isoelectric Point (pI)

34.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 41 - 459 4.5e-55 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1346
AccB1I GGYRCC 1 cut(s) 421
AccB7I CCANNNNNTGG 1 cut(s) 1415
AccI GTMKAC 3 cut(s) 32, 764, 1155
AciI CCGC 2 cut(s) 876, 1247
AcoI YGGCCR 3 cut(s) 140, 567, 873
AcsI RAATTY 4 cut(s) 188, 287, 1284, 1317
AcuI CTGAAG 3 cut(s) 1001, 1067, 1442
AfiI CCNNNNNNNGG 4 cut(s) 149, 1085, 1297, 1415
AgsI TTSAA 4 cut(s) 232, 886, 1224, 1354
AjnI CCWGG 2 cut(s) 124, 447
AjuI GAANNNNNNNTTGG 4 cut(s) 435, 467, 1037, 1069
AluBI AGCT 9 cut(s) 132, 313, 349, 374, 547, 613, 896, 1439, 1454
AluI AGCT 9 cut(s) 132, 313, 349, 374, 547, 613, 896, 1439, 1454
Alw21I GWGCWC 1 cut(s) 1172
Alw26I GTCTC 3 cut(s) 322, 995, 1436
AlwNI CAGNNNCTG 1 cut(s) 550
AoxI GGCC 3 cut(s) 140, 567, 873
ApeKI GCWGC 7 cut(s) 129, 320, 374, 544, 701, 710, 1103
ApoI RAATTY 4 cut(s) 188, 287, 1284, 1317
ArsI GACNNNNNNTTYG 2 cut(s) 507, 539
AseI ATTAAT 1 cut(s) 114
Asp700I GAANNNNTTC 1 cut(s) 1194
AspLEI GCGC 3 cut(s) 646, 706, 1204
AspS9I GGNCC 1 cut(s) 1277
AsuC2I CCSGG 3 cut(s) 426, 672, 1293
AsuHPI GGTGA 1 cut(s) 260
AvaII GGWCC 1 cut(s) 1277
BanI GGYRCC 1 cut(s) 421
Bbv12I GWGCWC 1 cut(s) 1172
BbvI GCAGC 7 cut(s) 141, 332, 361, 556, 697, 713, 1090
BccI CCATC 8 cut(s) 53, 392, 463, 503, 619, 1140, 1344, 1409
BcgI CGANNNNNNTGC 2 cut(s) 143, 177
BciT130I CCWGG 2 cut(s) 126, 449
BcnI CCSGG 3 cut(s) 426, 672, 1293
BcoDI GTCTC 3 cut(s) 322, 995, 1436
BfaI CTAG 1 cut(s) 245
BfoI RGCGCY 2 cut(s) 647, 707
BglI GCCNNNNNGGC 1 cut(s) 1058
BisI GCNGC 8 cut(s) 130, 321, 375, 545, 702, 711, 876, 1104
BlsI GCNGC 8 cut(s) 131, 322, 376, 546, 703, 712, 877, 1105
Bme1390I CCNGG 5 cut(s) 126, 426, 449, 672, 1293
Bme18I GGWCC 1 cut(s) 1277
BmgT120I GGNCC 1 cut(s) 1277
BmiI GGNNCC 2 cut(s) 423, 772
BmrFI CCNGG 5 cut(s) 126, 426, 449, 672, 1293
BmrI ACTGGG 1 cut(s) 514
BmsI GCATC 1 cut(s) 627
BmuI ACTGGG 1 cut(s) 514
BplI GAGNNNNNCTC 2 cut(s) 1264, 1296
BpmI CTGGAG 2 cut(s) 876, 992
BpuMI CCSGG 3 cut(s) 426, 672, 1293
BsaAI YACGTR 1 cut(s) 460
BsaI GGTCTC 1 cut(s) 322
BsaJI CCNNGG 9 cut(s) 124, 258, 425, 447, 477, 671, 1053, 1123, 1291
BsaWI WCCGGW 2 cut(s) 767, 821
Bsc4I CCNNNNNNNGG 4 cut(s) 149, 1085, 1297, 1415
Bse118I RCCGGY 2 cut(s) 278, 706
Bse1I ACTGG 5 cut(s) 509, 555, 975, 1163, 1409
Bse3DI GCAATG 1 cut(s) 699
BseBI CCWGG 2 cut(s) 126, 449
BseDI CCNNGG 9 cut(s) 124, 258, 425, 447, 477, 671, 1053, 1123, 1291
BseGI GGATG 5 cut(s) 210, 514, 1021, 1151, 1260
BseLI CCNNNNNNNGG 4 cut(s) 149, 1085, 1297, 1415
BseMI GCAATG 1 cut(s) 699
BseMII CTCAG 1 cut(s) 431
BseNI ACTGG 5 cut(s) 509, 555, 975, 1163, 1409
BseRI GAGGAG 2 cut(s) 208, 1233
BseXI GCAGC 7 cut(s) 141, 332, 361, 556, 697, 713, 1090
BshFI GGCC 3 cut(s) 142, 569, 875
BshNI GGYRCC 1 cut(s) 421
BsiHKAI GWGCWC 1 cut(s) 1172
BsiSI CCGG 7 cut(s) 279, 425, 671, 707, 768, 822, 1293
BslFI GGGAC 2 cut(s) 597, 688
BslI CCNNNNNNNGG 4 cut(s) 149, 1085, 1297, 1415
BsmAI GTCTC 3 cut(s) 322, 995, 1436
BsmBI CGTCTC 1 cut(s) 1436
BsmFI GGGAC 2 cut(s) 597, 688
BsmI GAATGC 1 cut(s) 68
BsnI GGCC 3 cut(s) 142, 569, 875
Bso31I GGTCTC 1 cut(s) 322
Bsp1286I GDGCHC 1 cut(s) 1172
Bsp143I GATC 2 cut(s) 784, 1383
Bsp19I CCATGG 1 cut(s) 477
BspACI CCGC 2 cut(s) 876, 1247
BspANI GGCC 3 cut(s) 142, 569, 875
BspCNI CTCAG 1 cut(s) 430
BspLI GGNNCC 2 cut(s) 423, 772
BspT107I GGYRCC 1 cut(s) 421
BspTNI GGTCTC 1 cut(s) 322
BsrDI GCAATG 1 cut(s) 699
BsrFI RCCGGY 2 cut(s) 278, 706
BsrI ACTGG 5 cut(s) 509, 555, 975, 1163, 1409
BssAI RCCGGY 2 cut(s) 278, 706
BssECI CCNNGG 9 cut(s) 124, 258, 425, 447, 477, 671, 1053, 1123, 1291
BssMI GATC 2 cut(s) 784, 1383
BssT1I CCWWGG 3 cut(s) 477, 1053, 1123
Bst2UI CCWGG 2 cut(s) 126, 449
Bst4CI ACNGT 3 cut(s) 11, 259, 1165
Bst6I CTCTTC 2 cut(s) 1086, 1203
BstBAI YACGTR 1 cut(s) 460
BstC8I GCNNGC 3 cut(s) 438, 642, 708
BstDEI CTNAG 2 cut(s) 417, 1322
BstDSI CCRYGG 2 cut(s) 258, 477
BstF5I GGATG 5 cut(s) 210, 514, 1021, 1151, 1260
BstH2I RGCGCY 2 cut(s) 647, 707
BstHHI GCGC 3 cut(s) 646, 706, 1204
BstKTI GATC 2 cut(s) 787, 1386
BstMAI GTCTC 3 cut(s) 322, 995, 1436
BstMBI GATC 2 cut(s) 784, 1383
BstMWI GCNNNNNNNGC 4 cut(s) 698, 707, 710, 1058
BstNI CCWGG 2 cut(s) 126, 449
BstNSI RCATGY 1 cut(s) 413
BstSCI CCNGG 5 cut(s) 124, 424, 447, 670, 1291
BstV1I GCAGC 7 cut(s) 141, 332, 361, 556, 697, 713, 1090
BstXI CCANNNNNNTGG 1 cut(s) 640
BsuRI GGCC 3 cut(s) 142, 569, 875
BtgI CCRYGG 2 cut(s) 258, 477
BtgZI GCGATG 2 cut(s) 518, 1076
BtsCI GGATG 5 cut(s) 210, 514, 1021, 1151, 1260
BtsI GCAGTG 1 cut(s) 273
BtsIMutI CAGTG 3 cut(s) 273, 502, 1170
Cac8I GCNNGC 3 cut(s) 438, 642, 708
CaiI CAGNNNCTG 1 cut(s) 550
CfoI GCGC 3 cut(s) 646, 706, 1204
Cfr10I RCCGGY 2 cut(s) 278, 706
Cfr13I GGNCC 1 cut(s) 1277
DdeI CTNAG 2 cut(s) 417, 1322
DpnI GATC 2 cut(s) 786, 1385
DpnII GATC 2 cut(s) 784, 1383
EaeI YGGCCR 3 cut(s) 140, 567, 873
Eam1104I CTCTTC 2 cut(s) 1086, 1203
EarI CTCTTC 2 cut(s) 1086, 1203
EciI GGCGGA 1 cut(s) 1236
Eco130I CCWWGG 3 cut(s) 477, 1053, 1123
Eco31I GGTCTC 1 cut(s) 322
Eco47I GGWCC 1 cut(s) 1277
Eco57I CTGAAG 3 cut(s) 1001, 1067, 1442
EcoRII CCWGG 2 cut(s) 124, 447
EcoT14I CCWWGG 3 cut(s) 477, 1053, 1123
EcoT22I ATGCAT 1 cut(s) 783
ErhI CCWWGG 3 cut(s) 477, 1053, 1123
Esp3I CGTCTC 1 cut(s) 1436
FaqI GGGAC 2 cut(s) 597, 688
FblI GTMKAC 3 cut(s) 32, 764, 1155
Fnu4HI GCNGC 8 cut(s) 130, 321, 375, 545, 702, 711, 876, 1104
FokI GGATG 5 cut(s) 217, 521, 1028, 1158, 1247
Fsp4HI GCNGC 8 cut(s) 130, 321, 375, 545, 702, 711, 876, 1104
FspBI CTAG 1 cut(s) 245
GlaI GCGC 3 cut(s) 645, 705, 1203
GluI GCNGC 8 cut(s) 130, 321, 375, 545, 702, 711, 876, 1104
GsuI CTGGAG 2 cut(s) 876, 992
HaeII RGCGCY 2 cut(s) 647, 707
HaeIII GGCC 3 cut(s) 142, 569, 875
HapII CCGG 7 cut(s) 279, 425, 671, 707, 768, 822, 1293
HhaI GCGC 3 cut(s) 646, 706, 1204
Hin6I GCGC 3 cut(s) 644, 704, 1202
HinP1I GCGC 3 cut(s) 644, 704, 1202
HindIII AAGCTT 2 cut(s) 611, 1452
HinfI GANTC 7 cut(s) 473, 559, 585, 761, 976, 1350, 1399
HpaII CCGG 7 cut(s) 279, 425, 671, 707, 768, 822, 1293
HphI GGTGA 1 cut(s) 260
Hpy166II GTNNAC 4 cut(s) 33, 765, 1156, 1369
Hpy188I TCNGA 2 cut(s) 959, 981
Hpy188III TCNNGA 4 cut(s) 485, 916, 1231, 1270
Hpy8I GTNNAC 4 cut(s) 33, 765, 1156, 1369
HpyAV CCTTC 5 cut(s) 175, 819, 1144, 1230, 1417
HpyCH4III ACNGT 3 cut(s) 11, 259, 1165
HpyCH4IV ACGT 2 cut(s) 459, 1047
HpyF10VI GCNNNNNNNGC 4 cut(s) 698, 707, 710, 1058
HpyF3I CTNAG 2 cut(s) 417, 1322
HpySE526I ACGT 2 cut(s) 459, 1047
HspAI GCGC 3 cut(s) 644, 704, 1202
KroI GCCGGC 1 cut(s) 706
KroNI GCCGGC 1 cut(s) 708
Kzo9I GATC 2 cut(s) 784, 1383
Lsp1109I GCAGC 7 cut(s) 141, 332, 361, 556, 697, 713, 1090
LweI GCATC 1 cut(s) 627
MaeI CTAG 1 cut(s) 245
MaeII ACGT 2 cut(s) 459, 1047
MaeIII GTNAC 2 cut(s) 11, 281
MalI GATC 2 cut(s) 786, 1385
MboI GATC 2 cut(s) 784, 1383
MfeI CAATTG 1 cut(s) 1009
MhlI GDGCHC 1 cut(s) 1172
MlyI GAGTC 3 cut(s) 553, 579, 770
MmeI TCCRAC 1 cut(s) 324
Mph1103I ATGCAT 1 cut(s) 783
MroNI GCCGGC 1 cut(s) 706
MroXI GAANNNNTTC 1 cut(s) 1194
MseI TTAA 3 cut(s) 114, 738, 1456
MslI CAYNNNNRTG 4 cut(s) 386, 507, 635, 1178
MspI CCGG 7 cut(s) 279, 425, 671, 707, 768, 822, 1293
MspR9I CCNGG 5 cut(s) 126, 426, 449, 672, 1293
MunI CAATTG 1 cut(s) 1009
Mva1269I GAATGC 1 cut(s) 68
MvaI CCWGG 2 cut(s) 126, 449
MwoI GCNNNNNNNGC 4 cut(s) 698, 707, 710, 1058
NaeI GCCGGC 1 cut(s) 708
NciI CCSGG 3 cut(s) 426, 672, 1293
NcoI CCATGG 1 cut(s) 477
NdeII GATC 2 cut(s) 784, 1383
NgoMIV GCCGGC 1 cut(s) 706
NlaIV GGNNCC 2 cut(s) 423, 772
NmeAIII GCCGAG 1 cut(s) 545
NmuCI GTSAC 1 cut(s) 281
NsiI ATGCAT 1 cut(s) 783
NspI RCATGY 1 cut(s) 413
PctI GAATGC 1 cut(s) 68
PdiI GCCGGC 1 cut(s) 708
PdmI GAANNNNTTC 1 cut(s) 1194
PfeI GAWTC 4 cut(s) 473, 976, 1350, 1399
PflFI GACNNNGTC 1 cut(s) 953
PflMI CCANNNNNTGG 1 cut(s) 1415
PkrI GCNGC 8 cut(s) 131, 322, 376, 546, 703, 712, 877, 1105
PleI GAGTC 3 cut(s) 553, 579, 769
PpsI GAGTC 3 cut(s) 553, 579, 769
Ppu21I YACGTR 1 cut(s) 460
PshBI ATTAAT 1 cut(s) 114
PsiI TTATAA 1 cut(s) 1346
Psp6I CCWGG 2 cut(s) 124, 447
PspGI CCWGG 2 cut(s) 124, 447
PspN4I GGNNCC 2 cut(s) 423, 772
PspPI GGNCC 1 cut(s) 1277
PstNI CAGNNNCTG 1 cut(s) 550
PsyI GACNNNGTC 1 cut(s) 953
RseI CAYNNNNRTG 4 cut(s) 386, 507, 635, 1178
SaqAI TTAA 3 cut(s) 114, 738, 1456
SatI GCNGC 8 cut(s) 130, 321, 375, 545, 702, 711, 876, 1104
Sau3AI GATC 2 cut(s) 784, 1383
Sau96I GGNCC 1 cut(s) 1277
SchI GAGTC 3 cut(s) 553, 579, 770
ScrFI CCNGG 5 cut(s) 126, 426, 449, 672, 1293
SduI GDGCHC 1 cut(s) 1172
SfaNI GCATC 1 cut(s) 627
SinI GGWCC 1 cut(s) 1277
SmiMI CAYNNNNRTG 4 cut(s) 386, 507, 635, 1178
SsiI CCGC 2 cut(s) 876, 1247
SspI AATATT 2 cut(s) 667, 1193
SspMI CTAG 1 cut(s) 245
StyD4I CCNGG 5 cut(s) 124, 424, 447, 670, 1291
StyI CCWWGG 3 cut(s) 477, 1053, 1123
TaaI ACNGT 3 cut(s) 11, 259, 1165
TaiI ACGT 2 cut(s) 462, 1050
TaqI TCGA 6 cut(s) 486, 525, 588, 618, 1230, 1282
TauI GCSGC 1 cut(s) 878
TfiI GAWTC 4 cut(s) 473, 976, 1350, 1399
Tru1I TTAA 3 cut(s) 114, 738, 1456
Tru9I TTAA 3 cut(s) 114, 738, 1456
TscAI CASTG 3 cut(s) 280, 509, 1170
TseFI GTSAC 1 cut(s) 281
TseI GCWGC 7 cut(s) 129, 320, 374, 544, 701, 710, 1103
Tsp45I GTSAC 1 cut(s) 281
TspRI CASTG 3 cut(s) 280, 509, 1170
Tth111I GACNNNGTC 1 cut(s) 953
Van91I CCANNNNNTGG 1 cut(s) 1415
VpaK11BI GGWCC 1 cut(s) 1277
VspI ATTAAT 1 cut(s) 114
XapI RAATTY 4 cut(s) 188, 287, 1284, 1317
XceI RCATGY 1 cut(s) 413
XcmI CCANNNNNNNNNTGG 1 cut(s) 577
XmiI GTMKAC 3 cut(s) 32, 764, 1155
XmnI GAANNNNTTC 1 cut(s) 1194
XspI CTAG 1 cut(s) 245
Zsp2I ATGCAT 1 cut(s) 783
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.