MD13G1029100.v1.1

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0000778
Physical Location & Seq
Reverse (-)
2075984 .. 2077773
1790 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1029100.v1.1.491

Sequence Viewer

Length: 1425 bp
ATGGAGACTCAATTATTATTGTTGTTATTACTGAGTGCTCTTGCTCTCATCATTTATGCATTCAAATCTTCCAAAACCAACCTTCCACCGGGAAGTTTCGGATGGCCAATCATTGGCGAAACGATCGCCTTCATGAAAGAGCAGCAACAACACTTTGTGGGCGAAAGAATGAAAAAACACTCCACAAAGCTCTTTAAAACCAGCATATTCGGCGAGCGGATGGTTGTTTTATGCGGCACTGCCGGTCACAAGTTCATCGCCACCAACGAGGAAAAGCTTTTTCTTGCATGGCGACCACTATCCATGCAGAAGCTCTTCCGCTCTTCCTACCTAAAAGCCGCTGCCACAGCCATCGTACCGCAAGCAGACGTAATCCAAATCTACCGTGCCCCGGGCTTTTTGAAGCCCGAGGCTCTGGTGAAGTACATAGAAACTATGGACTCTTTGGTTCAAGAACATTTGAAGCTTCACTGGGAAGGCAAAACCACGGTTGAGGTATATAAACTTGCTCAGCTACTTGTCACGAGTTTGTCCGCTCGCTTCTTCACTGGACTGCATGACAATGAGCGCACGGAAAAGTATGCCCAGTTGATGGATGTTCTCACTTCAGGGCTGCATACTATTCACATCAACATTCCCGGAACCACGTTTCATCGAGCAATGAAGGCGGCGAAGGCATTGAGGAAGGAGGTTTTGTTGTTGATTGAGGAGAAGAAAGCGGCTGCTGCCAGTGGAGTCCAAACGCACGACATCTTGTCTTTCATGCTATTTAACCCGGACCCCACTGGAAGGTTCTCATCGGAGAATGAAGTTGCTGATAAATTTATGGGTTCGATGGTGGCTGCGTTTCACTCACCTTCCATGGTTACAGGTTTCCTCTTCAAATATCTTGGAGAAAGACCTGAAATATGTGACAAAGTTCGTACAGAACAATTGGAGATCGCAAGTTCAAAAAAGTCTGGCGAGGCACTGATCTGGGAAGATATACACAAGATGAAATATTCCTGGGGTGTGGCACTTGAAGTTATGAGGCTGGTACCACCTCTCCAAGGAACATTTAGGGAGGTTGCTACTGAATTCACGTATGAAGGTTACACAATTCCAAAAGGGTGGAAGGTGTTTTGGTCGGCAAGCAGCACGAATAAAATCCCTGAATACTTTCCGGATCCGGAAGAGTTTGATCCGACTAGGTTTGAGAATGGAAAAACTCCTCCGACTTACTCTAACATCCCATTTGGCAGCGGCCCTCGAATTTGCCCGGGAAAAGAATATGCTAGACTCCAACTTATGTGCTCCCTTCATCACATTGTCACAAAATACAAGTGGGAAGTAATAAATCCAAACAGCAAGATTGCAGGTGGTTTGAATCCAGTTCCAGAAGAAGGCATTCACATTCGCATCCAACCATATTTGCCCCAACAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
p450

Protein Analysis

475

Amino Acids

53.61

Weight (kDa)

8.94

Isoelectric Point (pI)

34.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 29 - 447 7.7e-47 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1346
Acc36I ACCTGC 1 cut(s) 1346
Acc65I GGTACC 1 cut(s) 1036
AccB1I GGYRCC 1 cut(s) 1036
AccB7I CCANNNNNTGG 2 cut(s) 113, 592
AccBSI CCGCTC 3 cut(s) 217, 321, 536
AccIII TCCGGA 2 cut(s) 1162, 1168
AciI CCGC 9 cut(s) 217, 234, 319, 339, 359, 534, 668, 719, 1242
AclWI GGATC 3 cut(s) 1160, 1173, 1175
AcoI YGGCCR 1 cut(s) 104
AcsI RAATTY 3 cut(s) 821, 1076, 1251
AcuI CTGAAG 1 cut(s) 591
AdeI CACNNNGTG 1 cut(s) 157
AfaI GTAC 4 cut(s) 357, 425, 925, 1038
AfiI CCNNNNNNNGG 7 cut(s) 88, 113, 391, 592, 789, 1049, 1382
AgsI TTSAA 8 cut(s) 64, 403, 452, 463, 883, 951, 1022, 1366
AhdI GACNNNNNGTC 1 cut(s) 754
AjnI CCWGG 1 cut(s) 1004
AluBI AGCT 5 cut(s) 190, 277, 313, 466, 514
AluI AGCT 5 cut(s) 190, 277, 313, 466, 514
Alw21I GWGCWC 2 cut(s) 40, 1295
AlwI GGATC 3 cut(s) 1160, 1173, 1175
Ama87I CYCGRG 3 cut(s) 391, 407, 1258
Aor13HI TCCGGA 2 cut(s) 1162, 1168
AoxI GGCC 2 cut(s) 104, 1243
ApeKI GCWGC 8 cut(s) 142, 341, 613, 722, 725, 842, 1134, 1239
ApoI RAATTY 3 cut(s) 821, 1076, 1251
Asp700I GAANNNNTTC 3 cut(s) 314, 1158, 1386
Asp718I GGTACC 1 cut(s) 1036
AspLEI GCGC 1 cut(s) 570
AspS9I GGNCC 2 cut(s) 778, 1244
AsuC2I CCSGG 7 cut(s) 90, 392, 393, 639, 776, 1259, 1260
AsuHPI GGTGA 2 cut(s) 430, 846
AvaI CYCGRG 3 cut(s) 391, 407, 1258
AvaII GGWCC 1 cut(s) 778
BaeGI GKGCMC 1 cut(s) 391
BalI TGGCCA 1 cut(s) 106
BamHI GGATCC 1 cut(s) 1165
BanI GGYRCC 1 cut(s) 1036
BauI CACGAG 1 cut(s) 523
Bbv12I GWGCWC 2 cut(s) 40, 1295
BbvI GCAGC 8 cut(s) 154, 328, 600, 709, 712, 829, 1146, 1251
BccI CCATC 5 cut(s) 96, 214, 359, 586, 829
BciT130I CCWGG 1 cut(s) 1006
BcnI CCSGG 7 cut(s) 90, 392, 393, 639, 776, 1259, 1260
BfaI CTAG 2 cut(s) 1188, 1275
BfuAI ACCTGC 1 cut(s) 1346
BlpI GCTNAGC 1 cut(s) 510
Bme1390I CCNGG 8 cut(s) 90, 392, 393, 639, 776, 1006, 1259, 1260
Bme18I GGWCC 1 cut(s) 778
BmeRI GACNNNNNGTC 1 cut(s) 754
BmeT110I CYCGRG 3 cut(s) 391, 407, 1258
BmgT120I GGNCC 2 cut(s) 778, 1244
BmiI GGNNCC 4 cut(s) 643, 780, 1038, 1167
BmrFI CCNGG 8 cut(s) 90, 392, 393, 639, 776, 1006, 1259, 1260
BmrI ACTGGG 2 cut(s) 481, 580
BmsI GCATC 1 cut(s) 1407
BmuI ACTGGG 2 cut(s) 481, 580
Bpu1102I GCTNAGC 1 cut(s) 510
BpuMI CCSGG 7 cut(s) 90, 392, 393, 639, 776, 1259, 1260
BsaAI YACGTR 1 cut(s) 1083
BsaJI CCNNGG 8 cut(s) 390, 391, 408, 486, 861, 1005, 1048, 1258
BsaWI WCCGGW 2 cut(s) 1162, 1168
BsaXI ACNNNNNCTCC 4 cut(s) 680, 710, 1029, 1059
Bsc4I CCNNNNNNNGG 7 cut(s) 88, 113, 391, 592, 789, 1049, 1382
Bse118I RCCGGY 1 cut(s) 242
Bse1I ACTGG 6 cut(s) 476, 553, 586, 729, 790, 1370
Bse3DI GCAATG 1 cut(s) 666
BseAI TCCGGA 2 cut(s) 1162, 1168
BseBI CCWGG 1 cut(s) 1006
BseDI CCNNGG 8 cut(s) 390, 391, 408, 486, 861, 1005, 1048, 1258
BseGI GGATG 5 cut(s) 107, 225, 601, 1227, 1398
BseLI CCNNNNNNNGG 7 cut(s) 88, 113, 391, 592, 789, 1049, 1382
BseMI GCAATG 1 cut(s) 666
BseMII CTCAG 2 cut(s) 23, 524
BseNI ACTGG 6 cut(s) 476, 553, 586, 729, 790, 1370
BseRI GAGGAG 2 cut(s) 722, 1200
BseSI GKGCMC 1 cut(s) 391
BseXI GCAGC 8 cut(s) 154, 328, 600, 709, 712, 829, 1146, 1251
Bsh1285I CGRYCG 1 cut(s) 126
BshFI GGCC 2 cut(s) 106, 1245
BshNI GGYRCC 1 cut(s) 1036
BsiEI CGRYCG 1 cut(s) 126
BsiHKAI GWGCWC 2 cut(s) 40, 1295
BsiHKCI CYCGRG 3 cut(s) 391, 407, 1258
BsiSI CCGG 8 cut(s) 89, 243, 392, 639, 776, 1163, 1169, 1259
BslI CCNNNNNNNGG 7 cut(s) 88, 113, 391, 592, 789, 1049, 1382
BsmI GAATGC 2 cut(s) 59, 1386
BsnI GGCC 2 cut(s) 106, 1245
BsoBI CYCGRG 3 cut(s) 391, 407, 1258
Bsp1286I GDGCHC 3 cut(s) 40, 391, 1295
Bsp13I TCCGGA 2 cut(s) 1162, 1168
Bsp143I GATC 5 cut(s) 123, 939, 972, 1165, 1180
Bsp1720I GCTNAGC 1 cut(s) 510
Bsp19I CCATGG 1 cut(s) 861
BspACI CCGC 9 cut(s) 217, 234, 319, 339, 359, 534, 668, 719, 1242
BspANI GGCC 2 cut(s) 106, 1245
BspCNI CTCAG 2 cut(s) 24, 523
BspEI TCCGGA 2 cut(s) 1162, 1168
BspHI TCATGA 1 cut(s) 132
BspLI GGNNCC 4 cut(s) 643, 780, 1038, 1167
BspMI ACCTGC 1 cut(s) 1346
BspPI GGATC 3 cut(s) 1160, 1173, 1175
BspQI GCTCTTC 2 cut(s) 320, 328
BspT107I GGYRCC 1 cut(s) 1036
BsrBI CCGCTC 3 cut(s) 217, 321, 536
BsrDI GCAATG 1 cut(s) 666
BsrFI RCCGGY 1 cut(s) 242
BsrI ACTGG 6 cut(s) 476, 553, 586, 729, 790, 1370
BssAI RCCGGY 1 cut(s) 242
BssECI CCNNGG 8 cut(s) 390, 391, 408, 486, 861, 1005, 1048, 1258
BssMI GATC 5 cut(s) 123, 939, 972, 1165, 1180
BssSI CACGAG 1 cut(s) 523
BssT1I CCWWGG 2 cut(s) 861, 1048
Bst2BI CACGAG 1 cut(s) 523
Bst2UI CCWGG 1 cut(s) 1006
Bst4CI ACNGT 3 cut(s) 386, 490, 1422
Bst6I CTCTTC 4 cut(s) 320, 328, 884, 1167
BstBAI YACGTR 1 cut(s) 1083
BstC8I GCNNGC 4 cut(s) 215, 363, 538, 1132
BstDEI CTNAG 2 cut(s) 32, 510
BstDSI CCRYGG 2 cut(s) 486, 861
BstENI CCTNNNNNAGG 1 cut(s) 1047
BstF5I GGATG 5 cut(s) 107, 225, 601, 1227, 1398
BstHHI GCGC 1 cut(s) 570
BstKTI GATC 5 cut(s) 126, 942, 975, 1168, 1183
BstMBI GATC 5 cut(s) 123, 939, 972, 1165, 1180
BstMCI CGRYCG 1 cut(s) 126
BstMWI GCNNNNNNNGC 5 cut(s) 210, 347, 665, 674, 725
BstNI CCWGG 1 cut(s) 1006
BstSCI CCNGG 8 cut(s) 88, 390, 391, 637, 774, 1004, 1257, 1258
BstSLI GKGCMC 1 cut(s) 391
BstV1I GCAGC 8 cut(s) 154, 328, 600, 709, 712, 829, 1146, 1251
BstX2I RGATCY 1 cut(s) 1165
BstXI CCANNNNNNTGG 1 cut(s) 1110
BstYI RGATCY 1 cut(s) 1165
BsuRI GGCC 2 cut(s) 106, 1245
BtgI CCRYGG 2 cut(s) 486, 861
BtgZI GCGATG 1 cut(s) 241
BtsCI GGATG 5 cut(s) 107, 225, 601, 1227, 1398
BtsI GCAGTG 1 cut(s) 237
BtsIMutI CAGTG 6 cut(s) 237, 469, 546, 736, 783, 968
BveI ACCTGC 1 cut(s) 1346
Cac8I GCNNGC 4 cut(s) 215, 363, 538, 1132
CciI TCATGA 1 cut(s) 132
CfoI GCGC 1 cut(s) 570
Cfr10I RCCGGY 1 cut(s) 242
Cfr13I GGNCC 2 cut(s) 778, 1244
Cfr9I CCCGGG 2 cut(s) 391, 1258
Csp6I GTAC 4 cut(s) 356, 424, 924, 1037
CviAII CATG 6 cut(s) 133, 288, 304, 557, 763, 862
CviQI GTAC 4 cut(s) 356, 424, 924, 1037
DdeI CTNAG 2 cut(s) 32, 510
DpnI GATC 5 cut(s) 125, 941, 974, 1167, 1182
DpnII GATC 5 cut(s) 123, 939, 972, 1165, 1180
DraI TTTAAA 1 cut(s) 196
DraIII CACNNNGTG 1 cut(s) 157
DriI GACNNNNNGTC 1 cut(s) 754
EaeI YGGCCR 1 cut(s) 104
Eam1104I CTCTTC 4 cut(s) 320, 328, 884, 1167
Eam1105I GACNNNNNGTC 1 cut(s) 754
EarI CTCTTC 4 cut(s) 320, 328, 884, 1167
Eco130I CCWWGG 2 cut(s) 861, 1048
Eco47I GGWCC 1 cut(s) 778
Eco57I CTGAAG 1 cut(s) 591
Eco88I CYCGRG 3 cut(s) 391, 407, 1258
EcoNI CCTNNNNNAGG 1 cut(s) 1047
EcoRI GAATTC 1 cut(s) 1076
EcoRII CCWGG 1 cut(s) 1004
EcoT14I CCWWGG 2 cut(s) 861, 1048
EcoT22I ATGCAT 1 cut(s) 61
ErhI CCWWGG 2 cut(s) 861, 1048
FaeI CATG 6 cut(s) 136, 291, 307, 560, 766, 865
FatI CATG 6 cut(s) 132, 287, 303, 556, 762, 861
FokI GGATG 5 cut(s) 114, 232, 608, 1214, 1385
FspBI CTAG 2 cut(s) 1188, 1275
GlaI GCGC 1 cut(s) 569
HaeIII GGCC 2 cut(s) 106, 1245
HapII CCGG 8 cut(s) 89, 243, 392, 639, 776, 1163, 1169, 1259
HhaI GCGC 1 cut(s) 570
Hin1II CATG 6 cut(s) 136, 291, 307, 560, 766, 865
Hin6I GCGC 1 cut(s) 568
HinP1I GCGC 1 cut(s) 568
HindIII AAGCTT 2 cut(s) 275, 464
HinfI GANTC 5 cut(s) 7, 440, 735, 1278, 1366
HpaII CCGG 8 cut(s) 89, 243, 392, 639, 776, 1163, 1169, 1259
HphI GGTGA 2 cut(s) 430, 846
Hpy188I TCNGA 4 cut(s) 101, 802, 1185, 1215
Hpy188III TCNNGA 6 cut(s) 133, 452, 523, 1163, 1169, 1376
HpyCH4III ACNGT 3 cut(s) 386, 490, 1422
HpyCH4IV ACGT 3 cut(s) 369, 647, 1082
HpyCH4V TGCA 6 cut(s) 59, 287, 307, 556, 616, 1355
HpyF10VI GCNNNNNNNGC 5 cut(s) 210, 347, 665, 674, 725
HpyF3I CTNAG 2 cut(s) 32, 510
HpySE526I ACGT 3 cut(s) 369, 647, 1082
Hsp92II CATG 6 cut(s) 136, 291, 307, 560, 766, 865
HspAI GCGC 1 cut(s) 568
Kpn2I TCCGGA 2 cut(s) 1162, 1168
KpnI GGTACC 1 cut(s) 1040
Kzo9I GATC 5 cut(s) 123, 939, 972, 1165, 1180
LguI GCTCTTC 2 cut(s) 320, 328
LmnI GCTCC 1 cut(s) 1298
Lsp1109I GCAGC 8 cut(s) 154, 328, 600, 709, 712, 829, 1146, 1251
LweI GCATC 1 cut(s) 1407
MaeI CTAG 2 cut(s) 1188, 1275
MaeII ACGT 3 cut(s) 369, 647, 1082
MaeIII GTNAC 6 cut(s) 245, 520, 865, 911, 1091, 1309
MalI GATC 5 cut(s) 125, 941, 974, 1167, 1182
MbiI CCGCTC 3 cut(s) 217, 321, 536
MboI GATC 5 cut(s) 123, 939, 972, 1165, 1180
MboII GAAGA 9 cut(s) 60, 307, 315, 535, 724, 871, 992, 1184, 1391
MfeI CAATTG 1 cut(s) 932
MflI RGATCY 1 cut(s) 1165
MhlI GDGCHC 3 cut(s) 40, 391, 1295
MlsI TGGCCA 1 cut(s) 106
MluCI AATT 6 cut(s) 11, 821, 932, 1076, 1098, 1251
MluNI TGGCCA 1 cut(s) 106
MlyI GAGTC 3 cut(s) 434, 744, 1272
MmeI TCCRAC 3 cut(s) 1208, 1238, 1306
Mox20I TGGCCA 1 cut(s) 106
Mph1103I ATGCAT 1 cut(s) 61
MroI TCCGGA 2 cut(s) 1162, 1168
MroXI GAANNNNTTC 3 cut(s) 314, 1158, 1386
MscI TGGCCA 1 cut(s) 106
MseI TTAA 2 cut(s) 195, 771
MslI CAYNNNNRTG 1 cut(s) 561
Msp20I TGGCCA 1 cut(s) 106
MspA1I CMGCKG 2 cut(s) 341, 1242
MspI CCGG 8 cut(s) 89, 243, 392, 639, 776, 1163, 1169, 1259
MspR9I CCNGG 8 cut(s) 90, 392, 393, 639, 776, 1006, 1259, 1260
MunI CAATTG 1 cut(s) 932
Mva1269I GAATGC 2 cut(s) 59, 1386
MvaI CCWGG 1 cut(s) 1006
MwoI GCNNNNNNNGC 5 cut(s) 210, 347, 665, 674, 725
NciI CCSGG 7 cut(s) 90, 392, 393, 639, 776, 1259, 1260
NcoI CCATGG 1 cut(s) 861
NdeII GATC 5 cut(s) 123, 939, 972, 1165, 1180
NlaIII CATG 6 cut(s) 136, 291, 307, 560, 766, 865
NlaIV GGNNCC 4 cut(s) 643, 780, 1038, 1167
NmuCI GTSAC 4 cut(s) 245, 520, 911, 1309
NsiI ATGCAT 1 cut(s) 61
PagI TCATGA 1 cut(s) 132
PaqCI CACCTGC 1 cut(s) 1346
PciSI GCTCTTC 2 cut(s) 320, 328
PcsI WCGNNNNNNNCGW 1 cut(s) 264
PctI GAATGC 2 cut(s) 59, 1386
PdmI GAANNNNTTC 3 cut(s) 314, 1158, 1386
PfeI GAWTC 1 cut(s) 1366
PflMI CCANNNNNTGG 2 cut(s) 113, 592
PfoI TCCNGGA 1 cut(s) 637
Ple19I CGATCG 1 cut(s) 126
PleI GAGTC 3 cut(s) 434, 743, 1272
PpsI GAGTC 3 cut(s) 434, 743, 1272
Ppu21I YACGTR 1 cut(s) 1083
Psp6I CCWGG 1 cut(s) 1004
PspGI CCWGG 1 cut(s) 1004
PspN4I GGNNCC 4 cut(s) 643, 780, 1038, 1167
PspPI GGNCC 2 cut(s) 778, 1244
PsuI RGATCY 1 cut(s) 1165
PvuI CGATCG 1 cut(s) 126
RsaI GTAC 4 cut(s) 357, 425, 925, 1038
RsaNI GTAC 4 cut(s) 356, 424, 924, 1037
RseI CAYNNNNRTG 1 cut(s) 561
SapI GCTCTTC 2 cut(s) 320, 328
SaqAI TTAA 2 cut(s) 195, 771
Sau3AI GATC 5 cut(s) 123, 939, 972, 1165, 1180
Sau96I GGNCC 2 cut(s) 778, 1244
SchI GAGTC 3 cut(s) 434, 744, 1272
ScrFI CCNGG 8 cut(s) 90, 392, 393, 639, 776, 1006, 1259, 1260
SduI GDGCHC 3 cut(s) 40, 391, 1295
SfaNI GCATC 1 cut(s) 1407
SinI GGWCC 1 cut(s) 778
SmaI CCCGGG 2 cut(s) 393, 1260
SmiMI CAYNNNNRTG 1 cut(s) 561
Sse9I AATT 6 cut(s) 11, 821, 932, 1076, 1098, 1251
SsiI CCGC 9 cut(s) 217, 234, 319, 339, 359, 534, 668, 719, 1242
SspI AATATT 1 cut(s) 1001
SspMI CTAG 2 cut(s) 1188, 1275
StyD4I CCNGG 8 cut(s) 88, 390, 391, 637, 774, 1004, 1257, 1258
StyI CCWWGG 2 cut(s) 861, 1048
TaaI ACNGT 3 cut(s) 386, 490, 1422
TaiI ACGT 3 cut(s) 372, 650, 1085
TaqI TCGA 3 cut(s) 655, 833, 1249
TasI AATT 6 cut(s) 11, 821, 932, 1076, 1098, 1251
TatI WGTACW 1 cut(s) 423
TauI GCSGC 5 cut(s) 237, 341, 671, 722, 1245
TfiI GAWTC 1 cut(s) 1366
Tru1I TTAA 2 cut(s) 195, 771
Tru9I TTAA 2 cut(s) 195, 771
TscAI CASTG 6 cut(s) 244, 476, 553, 736, 790, 975
TseFI GTSAC 4 cut(s) 245, 520, 911, 1309
TseI GCWGC 8 cut(s) 142, 341, 613, 722, 725, 842, 1134, 1239
Tsp45I GTSAC 4 cut(s) 245, 520, 911, 1309
TspGWI ACGGA 1 cut(s) 587
TspMI CCCGGG 2 cut(s) 391, 1258
TspRI CASTG 6 cut(s) 244, 476, 553, 736, 790, 975
Van91I CCANNNNNTGG 2 cut(s) 113, 592
VpaK11BI GGWCC 1 cut(s) 778
XagI CCTNNNNNAGG 1 cut(s) 1047
XapI RAATTY 3 cut(s) 821, 1076, 1251
XmaI CCCGGG 2 cut(s) 391, 1258
XmnI GAANNNNTTC 3 cut(s) 314, 1158, 1386
XspI CTAG 2 cut(s) 1188, 1275
Zsp2I ATGCAT 1 cut(s) 61
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.