Rw0G021530

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00992
OG0000778
Physical Location & Seq
Forward (+)
14688 .. 17113
2426 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G021530.1

Sequence Viewer

Length: 1257 bp
ATGAAGAAATACTCCTCCAAAATCTTCAAAACCAAGATACTGGGTGAGTCAACCGTGGTGTTATGTGGCACTGCCGGTCACAAGTTTGTGGCTTCCAACGAAGAAAAGCTCTTTGTAGTATGGAGACCTCACTCCATGCAAAAGCTCTTCCGCTCTTCTTACCAAAAATCTACTTCCACCGTCATACCAAGACAAAGCGAAATGCACGTAGTTAAGGCACCGGGGTTTCTGAGAGTCGAAGCCCTCGTTGGCTACGTGGTAGCGATGGATTCCATGGTTCTCGACCACCTGAAAACGCATTGGGATGGCAAACAGGTGGTTGAGGCTCATCGCCTCACGCAGATACTCATTTTGACTCTCTCGGCTAAGTTCTTCATGGGACTCGAAGACCATTGTCGTATTGAAAAGCTTGCGGAGTTGATGGACACCATGATGCTGGCGCTTCACACTATTCCTGTGAATATTCCGGGGACTGCATTTCATCAAGCAATGAAAGCAGCGCCGGCTGCGAGGGAAATTATTCAGTCGTTTGTTAAGGAGAAGAAGGAAGTTATAGCGTCTACCGGAAACAAAATGCATGATCTTTTGTCTTATATGATTTCTACGCCAGACCCGGTTACCGGAAGGTTCATGCCGGAGAATGAAATTGCTGATAAAATTATGGGGAATGTGGCCGCTTCGTTCAACTCTCCAGCTATGACTACTACTTTTATCGTGAAGTTCTTGGGCGAAAGACCTCACATTTATGATAAAGTCCGGATTGAACAACTGGAGATTTTGAAGTCCAAAAAATCTGGTGAGGCACTCAATTGGGATGATATGCAAAAAATGAAGTATTCGTGGAACGTAGCCCTGGAGGCGATGAGGCTCATACCACCTCTTCAGGGAACATTTAGAGAGGCTGCAACAGACTTCACTTACCAAGGTTATACAATTCCTAAAGGATGGAAGGTCTACTGGACAGTGAGCACCACAACCATGAACCCTGAATATTTTCCAGCCCCAGAAGAGTTTGATCCTACAAGGTTCGAGAAAGCAAGTCTTCCGCCTTATACAAACATCCCATTCGGGAGCGGTCCTCGAATTTGCCCCGGAAAGGACTACGCTCGTCTGCAAATTCTTAGTTTCATGCACCATCTTGTTATAAGATTCAAATGGGAACTTGTGAACCCTAATTGCAAGATCACAGGTGGAATGAATCCCTTTCCAGTTGATGGGCTGAAGGTTCGCCTCCAAGCTCAACCCAGTCAAGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
p450

Protein Analysis

418

Amino Acids

47.38

Weight (kDa)

9.28

Isoelectric Point (pI)

41.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 2 - 390 2.9e-50 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1145
AccB1I GGYRCC 1 cut(s) 217
AccB7I CCANNNNNTGG 1 cut(s) 1214
AccBSI CCGCTC 2 cut(s) 153, 1074
AccI GTMKAC 2 cut(s) 560, 954
AccIII TCCGGA 1 cut(s) 756
AciI CCGC 5 cut(s) 151, 413, 675, 1046, 1074
AclWI GGATC 1 cut(s) 1010
AcoI YGGCCR 1 cut(s) 672
AcsI RAATTY 2 cut(s) 1083, 1116
AcuI CTGAAG 2 cut(s) 866, 1241
AfiI CCNNNNNNNGG 4 cut(s) 620, 884, 1096, 1214
AgsI TTSAA 6 cut(s) 28, 404, 685, 764, 781, 1153
AjnI CCWGG 1 cut(s) 852
AjuI GAANNNNNNNTTGG 2 cut(s) 231, 263
AluBI AGCT 6 cut(s) 109, 145, 409, 695, 1238, 1253
AluI AGCT 6 cut(s) 109, 145, 409, 695, 1238, 1253
Alw21I GWGCWC 1 cut(s) 971
Alw26I GTCTC 1 cut(s) 118
AlwI GGATC 1 cut(s) 1010
Aor13HI TCCGGA 1 cut(s) 756
AoxI GGCC 1 cut(s) 672
ApeKI GCWGC 3 cut(s) 497, 506, 902
ApoI RAATTY 2 cut(s) 1083, 1116
Asp700I GAANNNNTTC 2 cut(s) 519, 993
AspLEI GCGC 2 cut(s) 442, 502
AspS9I GGNCC 1 cut(s) 1076
AsuC2I CCSGG 4 cut(s) 222, 468, 614, 1092
AsuHPI GGTGA 2 cut(s) 56, 809
AvaII GGWCC 1 cut(s) 1076
BanI GGYRCC 1 cut(s) 217
BbsI GAAGAC 2 cut(s) 393, 1034
Bbv12I GWGCWC 1 cut(s) 971
BbvI GCAGC 3 cut(s) 493, 509, 889
BccI CCATC 6 cut(s) 259, 299, 415, 939, 1143, 1208
BciT130I CCWGG 1 cut(s) 854
BcnI CCSGG 4 cut(s) 222, 468, 614, 1092
BcoDI GTCTC 1 cut(s) 118
BfoI RGCGCY 2 cut(s) 443, 503
BglI GCCNNNNNGGC 1 cut(s) 857
BisI GCNGC 4 cut(s) 498, 507, 675, 903
BlsI GCNGC 4 cut(s) 499, 508, 676, 904
Bme1390I CCNGG 5 cut(s) 222, 468, 614, 854, 1092
Bme18I GGWCC 1 cut(s) 1076
BmgT120I GGNCC 1 cut(s) 1076
BmiI GGNNCC 1 cut(s) 219
BmrFI CCNGG 5 cut(s) 222, 468, 614, 854, 1092
BmrI ACTGGG 2 cut(s) 50, 1239
BmsI GCATC 1 cut(s) 423
BmuI ACTGGG 2 cut(s) 50, 1239
BoxI GACNNNNGTC 1 cut(s) 393
BpiI GAAGAC 2 cut(s) 393, 1034
BplI GAGNNNNNCTC 2 cut(s) 1063, 1095
BpmI CTGGAG 3 cut(s) 675, 791, 875
BpuMI CCSGG 4 cut(s) 222, 468, 614, 1092
BsaAI YACGTR 2 cut(s) 208, 256
BsaI GGTCTC 1 cut(s) 118
BsaJI CCNNGG 7 cut(s) 54, 221, 273, 467, 852, 922, 1090
BsaWI WCCGGW 3 cut(s) 563, 620, 756
Bsc4I CCNNNNNNNGG 4 cut(s) 620, 884, 1096, 1214
Bse118I RCCGGY 2 cut(s) 74, 502
Bse1I ACTGG 5 cut(s) 45, 774, 962, 1208, 1245
Bse3DI GCAATG 1 cut(s) 495
BseAI TCCGGA 1 cut(s) 756
BseBI CCWGG 1 cut(s) 854
BseDI CCNNGG 7 cut(s) 54, 221, 273, 467, 852, 922, 1090
BseGI GGATG 4 cut(s) 310, 820, 950, 1059
BseLI CCNNNNNNNGG 4 cut(s) 620, 884, 1096, 1214
BseMI GCAATG 1 cut(s) 495
BseMII CTCAG 1 cut(s) 221
BseNI ACTGG 5 cut(s) 45, 774, 962, 1208, 1245
BseRI GAGGAG 1 cut(s) 4
BseXI GCAGC 3 cut(s) 493, 509, 889
BshFI GGCC 1 cut(s) 674
BshNI GGYRCC 1 cut(s) 217
BsiHKAI GWGCWC 1 cut(s) 971
BslFI GGGAC 2 cut(s) 393, 484
BslI CCNNNNNNNGG 4 cut(s) 620, 884, 1096, 1214
BsmAI GTCTC 1 cut(s) 118
BsmFI GGGAC 2 cut(s) 393, 484
BsnI GGCC 1 cut(s) 674
Bso31I GGTCTC 1 cut(s) 118
Bsp1286I GDGCHC 1 cut(s) 971
Bsp13I TCCGGA 1 cut(s) 756
Bsp143I GATC 3 cut(s) 580, 1015, 1182
Bsp19I CCATGG 1 cut(s) 273
BspACI CCGC 5 cut(s) 151, 413, 675, 1046, 1074
BspANI GGCC 1 cut(s) 674
BspCNI CTCAG 1 cut(s) 222
BspEI TCCGGA 1 cut(s) 756
BspLI GGNNCC 1 cut(s) 219
BspPI GGATC 1 cut(s) 1010
BspQI GCTCTTC 2 cut(s) 152, 160
BspT107I GGYRCC 1 cut(s) 217
BspTNI GGTCTC 1 cut(s) 118
BsrBI CCGCTC 2 cut(s) 153, 1074
BsrDI GCAATG 1 cut(s) 495
BsrFI RCCGGY 2 cut(s) 74, 502
BsrI ACTGG 5 cut(s) 45, 774, 962, 1208, 1245
BssAI RCCGGY 2 cut(s) 74, 502
BssECI CCNNGG 7 cut(s) 54, 221, 273, 467, 852, 922, 1090
BssMI GATC 3 cut(s) 580, 1015, 1182
BssT1I CCWWGG 2 cut(s) 273, 922
Bst2UI CCWGG 1 cut(s) 854
Bst4CI ACNGT 3 cut(s) 55, 181, 964
Bst6I CTCTTC 4 cut(s) 152, 160, 885, 1002
BstBAI YACGTR 2 cut(s) 208, 256
BstC8I GCNNGC 3 cut(s) 411, 438, 504
BstDEI CTNAG 3 cut(s) 230, 366, 1121
BstDSI CCRYGG 2 cut(s) 54, 273
BstEII GGTNACC 1 cut(s) 616
BstF5I GGATG 4 cut(s) 310, 820, 950, 1059
BstH2I RGCGCY 2 cut(s) 443, 503
BstHHI GCGC 2 cut(s) 442, 502
BstKTI GATC 3 cut(s) 583, 1018, 1185
BstMAI GTCTC 1 cut(s) 118
BstMBI GATC 3 cut(s) 580, 1015, 1182
BstMWI GCNNNNNNNGC 4 cut(s) 494, 503, 506, 857
BstNI CCWGG 1 cut(s) 854
BstPAI GACNNNNGTC 1 cut(s) 393
BstPI GGTNACC 1 cut(s) 616
BstSCI CCNGG 5 cut(s) 220, 466, 612, 852, 1090
BstV1I GCAGC 3 cut(s) 493, 509, 889
BstV2I GAAGAC 2 cut(s) 393, 1034
BstXI CCANNNNNNTGG 2 cut(s) 40, 436
BsuRI GGCC 1 cut(s) 674
BtgI CCRYGG 2 cut(s) 54, 273
BtgZI GCGATG 3 cut(s) 278, 314, 875
BtsCI GGATG 4 cut(s) 310, 820, 950, 1059
BtsI GCAGTG 1 cut(s) 69
BtsIMutI CAGTG 2 cut(s) 69, 969
Cac8I GCNNGC 3 cut(s) 411, 438, 504
CfoI GCGC 2 cut(s) 442, 502
Cfr10I RCCGGY 2 cut(s) 74, 502
Cfr13I GGNCC 1 cut(s) 1076
CseI GACGC 1 cut(s) 546
CviAII CATG 8 cut(s) 136, 274, 376, 430, 578, 631, 979, 1129
DdeI CTNAG 3 cut(s) 230, 366, 1121
DpnI GATC 3 cut(s) 582, 1017, 1184
DpnII GATC 3 cut(s) 580, 1015, 1182
EaeI YGGCCR 1 cut(s) 672
Eam1104I CTCTTC 4 cut(s) 152, 160, 885, 1002
EarI CTCTTC 4 cut(s) 152, 160, 885, 1002
EciI GGCGGA 1 cut(s) 1035
Eco130I CCWWGG 2 cut(s) 273, 922
Eco31I GGTCTC 1 cut(s) 118
Eco47I GGWCC 1 cut(s) 1076
Eco57I CTGAAG 2 cut(s) 866, 1241
Eco91I GGTNACC 1 cut(s) 616
EcoO65I GGTNACC 1 cut(s) 616
EcoRII CCWGG 1 cut(s) 852
EcoT14I CCWWGG 2 cut(s) 273, 922
EcoT22I ATGCAT 1 cut(s) 579
ErhI CCWWGG 2 cut(s) 273, 922
FaeI CATG 8 cut(s) 139, 277, 379, 433, 581, 634, 982, 1132
FalI AAGNNNNNCTT 2 cut(s) 1026, 1058
FaqI GGGAC 2 cut(s) 393, 484
FatI CATG 8 cut(s) 135, 273, 375, 429, 577, 630, 978, 1128
FblI GTMKAC 2 cut(s) 560, 954
Fnu4HI GCNGC 4 cut(s) 498, 507, 675, 903
FokI GGATG 4 cut(s) 317, 827, 957, 1046
Fsp4HI GCNGC 4 cut(s) 498, 507, 675, 903
GlaI GCGC 2 cut(s) 441, 501
GluI GCNGC 4 cut(s) 498, 507, 675, 903
GsuI CTGGAG 3 cut(s) 675, 791, 875
HaeII RGCGCY 2 cut(s) 443, 503
HaeIII GGCC 1 cut(s) 674
HgaI GACGC 1 cut(s) 546
HhaI GCGC 2 cut(s) 442, 502
Hin1II CATG 8 cut(s) 139, 277, 379, 433, 581, 634, 982, 1132
Hin6I GCGC 2 cut(s) 440, 500
HinP1I GCGC 2 cut(s) 440, 500
HincII GTYRAC 1 cut(s) 51
HindII GTYRAC 1 cut(s) 51
HindIII AAGCTT 2 cut(s) 407, 1251
HinfI GANTC 7 cut(s) 47, 234, 269, 355, 381, 1149, 1198
HphI GGTGA 2 cut(s) 56, 809
Hpy166II GTNNAC 4 cut(s) 51, 561, 955, 1168
Hpy188I TCNGA 1 cut(s) 231
Hpy188III TCNNGA 5 cut(s) 281, 715, 757, 1030, 1069
Hpy8I GTNNAC 4 cut(s) 51, 561, 955, 1168
HpyAV CCTTC 4 cut(s) 538, 618, 943, 1216
HpyCH4III ACNGT 3 cut(s) 55, 181, 964
HpyCH4IV ACGT 3 cut(s) 207, 255, 846
HpyCH4V TGCA 9 cut(s) 139, 205, 476, 577, 823, 905, 1114, 1132, 1179
HpyF10VI GCNNNNNNNGC 4 cut(s) 494, 503, 506, 857
HpyF3I CTNAG 3 cut(s) 230, 366, 1121
HpySE526I ACGT 3 cut(s) 207, 255, 846
Hsp92II CATG 8 cut(s) 139, 277, 379, 433, 581, 634, 982, 1132
HspAI GCGC 2 cut(s) 440, 500
Kpn2I TCCGGA 1 cut(s) 756
KroI GCCGGC 1 cut(s) 502
KroNI GCCGGC 1 cut(s) 504
Kzo9I GATC 3 cut(s) 580, 1015, 1182
LguI GCTCTTC 2 cut(s) 152, 160
LmnI GCTCC 1 cut(s) 1071
Lsp1109I GCAGC 3 cut(s) 493, 509, 889
LweI GCATC 1 cut(s) 423
MaeII ACGT 3 cut(s) 207, 255, 846
MaeIII GTNAC 2 cut(s) 77, 616
MalI GATC 3 cut(s) 582, 1017, 1184
MbiI CCGCTC 2 cut(s) 153, 1074
MboI GATC 3 cut(s) 580, 1015, 1182
MfeI CAATTG 1 cut(s) 808
MhlI GDGCHC 1 cut(s) 971
MluCI AATT 8 cut(s) 516, 645, 657, 808, 933, 1083, 1116, 1174
MlyI GAGTC 4 cut(s) 56, 243, 349, 375
MmeI TCCRAC 1 cut(s) 120
Mph1103I ATGCAT 1 cut(s) 579
MroI TCCGGA 1 cut(s) 756
MroNI GCCGGC 1 cut(s) 502
MroXI GAANNNNTTC 2 cut(s) 519, 993
MseI TTAA 3 cut(s) 213, 534, 1255
MslI CAYNNNNRTG 4 cut(s) 303, 431, 744, 977
MspR9I CCNGG 5 cut(s) 222, 468, 614, 854, 1092
MunI CAATTG 1 cut(s) 808
MvaI CCWGG 1 cut(s) 854
MwoI GCNNNNNNNGC 4 cut(s) 494, 503, 506, 857
NaeI GCCGGC 1 cut(s) 504
NciI CCSGG 4 cut(s) 222, 468, 614, 1092
NcoI CCATGG 1 cut(s) 273
NdeII GATC 3 cut(s) 580, 1015, 1182
NgoMIV GCCGGC 1 cut(s) 502
NlaIII CATG 8 cut(s) 139, 277, 379, 433, 581, 634, 982, 1132
NlaIV GGNNCC 1 cut(s) 219
NmeAIII GCCGAG 1 cut(s) 341
NmuCI GTSAC 1 cut(s) 77
NsiI ATGCAT 1 cut(s) 579
PciSI GCTCTTC 2 cut(s) 152, 160
PcsI WCGNNNNNNNCGW 2 cut(s) 243, 252
PdiI GCCGGC 1 cut(s) 504
PdmI GAANNNNTTC 2 cut(s) 519, 993
PfeI GAWTC 3 cut(s) 269, 1149, 1198
PflMI CCANNNNNTGG 1 cut(s) 1214
PkrI GCNGC 4 cut(s) 499, 508, 676, 904
PleI GAGTC 4 cut(s) 55, 242, 349, 375
PpsI GAGTC 4 cut(s) 55, 242, 349, 375
Ppu21I YACGTR 2 cut(s) 208, 256
PshAI GACNNNNGTC 1 cut(s) 393
PsiI TTATAA 1 cut(s) 1145
Psp6I CCWGG 1 cut(s) 852
PspEI GGTNACC 1 cut(s) 616
PspGI CCWGG 1 cut(s) 852
PspN4I GGNNCC 1 cut(s) 219
PspPI GGNCC 1 cut(s) 1076
RseI CAYNNNNRTG 4 cut(s) 303, 431, 744, 977
SapI GCTCTTC 2 cut(s) 152, 160
SaqAI TTAA 3 cut(s) 213, 534, 1255
SatI GCNGC 4 cut(s) 498, 507, 675, 903
Sau3AI GATC 3 cut(s) 580, 1015, 1182
Sau96I GGNCC 1 cut(s) 1076
SchI GAGTC 4 cut(s) 56, 243, 349, 375
ScrFI CCNGG 5 cut(s) 222, 468, 614, 854, 1092
SduI GDGCHC 1 cut(s) 971
SfaNI GCATC 1 cut(s) 423
SinI GGWCC 1 cut(s) 1076
SmiMI CAYNNNNRTG 4 cut(s) 303, 431, 744, 977
Sse9I AATT 8 cut(s) 516, 645, 657, 808, 933, 1083, 1116, 1174
SsiI CCGC 5 cut(s) 151, 413, 675, 1046, 1074
SspI AATATT 2 cut(s) 463, 992
StyD4I CCNGG 5 cut(s) 220, 466, 612, 852, 1090
StyI CCWWGG 2 cut(s) 273, 922
TaaI ACNGT 3 cut(s) 55, 181, 964
TaiI ACGT 3 cut(s) 210, 258, 849
TaqI TCGA 5 cut(s) 237, 282, 384, 1029, 1081
TasI AATT 8 cut(s) 516, 645, 657, 808, 933, 1083, 1116, 1174
TauI GCSGC 1 cut(s) 677
TfiI GAWTC 3 cut(s) 269, 1149, 1198
Tru1I TTAA 3 cut(s) 213, 534, 1255
Tru9I TTAA 3 cut(s) 213, 534, 1255
TscAI CASTG 2 cut(s) 76, 969
TseFI GTSAC 1 cut(s) 77
TseI GCWGC 3 cut(s) 497, 506, 902
Tsp45I GTSAC 1 cut(s) 77
TspRI CASTG 2 cut(s) 76, 969
Van91I CCANNNNNTGG 1 cut(s) 1214
VpaK11BI GGWCC 1 cut(s) 1076
XapI RAATTY 2 cut(s) 1083, 1116
XmiI GTMKAC 2 cut(s) 560, 954
XmnI GAANNNNTTC 2 cut(s) 519, 993
Zsp2I ATGCAT 1 cut(s) 579
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.