MD13G1030800.v1.1

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0000778
Physical Location & Seq
Reverse (-)
2215444 .. 2216836
1393 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1030800.v1.1.491

Sequence Viewer

Length: 1065 bp
ATGGAGACTCAATTATTATTGTTGTTATTACTGAGTGCTCTTGCTCTCATCATTTATGCATTCAAATCTTCCAAAACCAACCTTCCACCGGGAAGTTTCGGATGGCCAATCATTGGCGAAACGATCGCCTTCATGAAAGAGCAGCAACAACACTTTGTGGGCGAAAGAATGAAAAAACACTCCACAAAGCTCTTTAAAACCAGCATATTCGGCGAGCGGATGGTTGTTTTATGCGGCACTGCCGGTCACAAGTTCATCGCCACCAACGAGGAAAAGCTTTTTCTTGCATGGCGACCACTATCCATGCAGAAGCTCTTCCGCTCTTCCTACCTAAAAGCCGCTGCCACAGCCATCGTACCGCAAGCAGACGTAATCCAAATCTACCGTGCCCCGGGCTTTTTGAAGCCCGAGGCTCTGGTGAAGTACATAGAAACTATGGACTCTTTGGTTCAAGAACATTTGAAGCTTCACTGGGAAGGCAAAACCACGGTTGAGGTATATAAACTTGCTCAGCTACTTGTCACACGAGTTTGTCCGCTCGCTTCTTCACTGGACTGCATGACAATGAGCGCACGGAAAAAAAGCGGCTGCTGCCAGTGGAGTCCAAACGCACACGACATCTTGTCTTTCATGCTATTTAACCCGGACCCCACTGGAAGGTTCTCATCGGAGAATGAAGTTGCTGATAAATTTATGGGTTCGATGGTGGCTGCGTTTCACTCACCTTCCATGGTTACAGGTTTCCTCTTCAAATATCTTGGAGAAAGACCTGAAATATGTGACAAAGTTCGTACAGAACAATTGGAGATCGCAAGTTCAAAAAAGTCTGGCGAGGCACTGATCTGGGAAGATATACACAAGATGAAATATTCCTGGGGTGTGGCACTTGAAGTTATGAGGCTGGTACCACCTCTCCAAGGAACATTTAGGGAGGTTGCTACTGAATTCACGTATGAAGGTTACACAATTCCAAAAGGGTGGAAGGTGTTTTGGTCGGCAAGCAGCACGAATAAAATCCCTGAATACTTTCCGGATCCGGAAGAGTTTGATCCGACTAGGTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
p450

Protein Analysis

355

Amino Acids

40.19

Weight (kDa)

8.58

Isoelectric Point (pI)

30.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 206 - 354 2.4e-23 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 904
AccB1I GGYRCC 1 cut(s) 904
AccB7I CCANNNNNTGG 1 cut(s) 113
AccBSI CCGCTC 3 cut(s) 217, 321, 538
AccIII TCCGGA 2 cut(s) 1030, 1036
AciI CCGC 7 cut(s) 217, 234, 319, 339, 359, 536, 585
AclWI GGATC 3 cut(s) 1028, 1041, 1043
AcoI YGGCCR 1 cut(s) 104
AcsI RAATTY 2 cut(s) 689, 944
AdeI CACNNNGTG 1 cut(s) 157
AfaI GTAC 4 cut(s) 357, 425, 793, 906
AfiI CCNNNNNNNGG 5 cut(s) 88, 113, 391, 657, 917
AgsI TTSAA 7 cut(s) 64, 403, 452, 463, 751, 819, 890
AhdI GACNNNNNGTC 1 cut(s) 622
AjnI CCWGG 1 cut(s) 872
AluBI AGCT 5 cut(s) 190, 277, 313, 466, 514
AluI AGCT 5 cut(s) 190, 277, 313, 466, 514
Alw21I GWGCWC 1 cut(s) 40
AlwI GGATC 3 cut(s) 1028, 1041, 1043
Ama87I CYCGRG 2 cut(s) 391, 407
Aor13HI TCCGGA 2 cut(s) 1030, 1036
AoxI GGCC 1 cut(s) 104
ApeKI GCWGC 6 cut(s) 142, 341, 588, 591, 710, 1002
ApoI RAATTY 2 cut(s) 689, 944
Asp700I GAANNNNTTC 2 cut(s) 314, 1026
Asp718I GGTACC 1 cut(s) 904
AspLEI GCGC 1 cut(s) 572
AspS9I GGNCC 1 cut(s) 646
AsuC2I CCSGG 4 cut(s) 90, 392, 393, 644
AsuHPI GGTGA 2 cut(s) 430, 714
AvaI CYCGRG 2 cut(s) 391, 407
AvaII GGWCC 1 cut(s) 646
BaeGI GKGCMC 1 cut(s) 391
BalI TGGCCA 1 cut(s) 106
BamHI GGATCC 1 cut(s) 1033
BanI GGYRCC 1 cut(s) 904
BauI CACGAG 1 cut(s) 525
Bbv12I GWGCWC 1 cut(s) 40
BbvI GCAGC 6 cut(s) 154, 328, 575, 578, 697, 1014
BccI CCATC 4 cut(s) 96, 214, 359, 697
BciT130I CCWGG 1 cut(s) 874
BcnI CCSGG 4 cut(s) 90, 392, 393, 644
BfaI CTAG 1 cut(s) 1056
BisI GCNGC 9 cut(s) 143, 235, 339, 342, 586, 589, 592, 711, 1003
BlpI GCTNAGC 1 cut(s) 510
BlsI GCNGC 9 cut(s) 144, 236, 340, 343, 587, 590, 593, 712, 1004
Bme1390I CCNGG 5 cut(s) 90, 392, 393, 644, 874
Bme18I GGWCC 1 cut(s) 646
BmeRI GACNNNNNGTC 1 cut(s) 622
BmeT110I CYCGRG 2 cut(s) 391, 407
BmgT120I GGNCC 1 cut(s) 646
BmiI GGNNCC 3 cut(s) 648, 906, 1035
BmrFI CCNGG 5 cut(s) 90, 392, 393, 644, 874
BmrI ACTGGG 1 cut(s) 481
BmuI ACTGGG 1 cut(s) 481
Bpu1102I GCTNAGC 1 cut(s) 510
BpuMI CCSGG 4 cut(s) 90, 392, 393, 644
BsaAI YACGTR 1 cut(s) 951
BsaJI CCNNGG 7 cut(s) 390, 391, 408, 486, 729, 873, 916
BsaWI WCCGGW 2 cut(s) 1030, 1036
BsaXI ACNNNNNCTCC 2 cut(s) 897, 927
Bsc4I CCNNNNNNNGG 5 cut(s) 88, 113, 391, 657, 917
Bse118I RCCGGY 1 cut(s) 242
Bse1I ACTGG 4 cut(s) 476, 555, 595, 658
BseAI TCCGGA 2 cut(s) 1030, 1036
BseBI CCWGG 1 cut(s) 874
BseDI CCNNGG 7 cut(s) 390, 391, 408, 486, 729, 873, 916
BseGI GGATG 2 cut(s) 107, 225
BseLI CCNNNNNNNGG 5 cut(s) 88, 113, 391, 657, 917
BseMII CTCAG 2 cut(s) 23, 524
BseNI ACTGG 4 cut(s) 476, 555, 595, 658
BseSI GKGCMC 1 cut(s) 391
BseXI GCAGC 6 cut(s) 154, 328, 575, 578, 697, 1014
Bsh1285I CGRYCG 1 cut(s) 126
BshFI GGCC 1 cut(s) 106
BshNI GGYRCC 1 cut(s) 904
BsiEI CGRYCG 1 cut(s) 126
BsiHKAI GWGCWC 1 cut(s) 40
BsiHKCI CYCGRG 2 cut(s) 391, 407
BsiSI CCGG 6 cut(s) 89, 243, 392, 644, 1031, 1037
BslI CCNNNNNNNGG 5 cut(s) 88, 113, 391, 657, 917
BsmI GAATGC 1 cut(s) 59
BsnI GGCC 1 cut(s) 106
BsoBI CYCGRG 2 cut(s) 391, 407
Bsp1286I GDGCHC 2 cut(s) 40, 391
Bsp13I TCCGGA 2 cut(s) 1030, 1036
Bsp143I GATC 5 cut(s) 123, 807, 840, 1033, 1048
Bsp1720I GCTNAGC 1 cut(s) 510
Bsp19I CCATGG 1 cut(s) 729
BspACI CCGC 7 cut(s) 217, 234, 319, 339, 359, 536, 585
BspANI GGCC 1 cut(s) 106
BspCNI CTCAG 2 cut(s) 24, 523
BspEI TCCGGA 2 cut(s) 1030, 1036
BspHI TCATGA 1 cut(s) 132
BspLI GGNNCC 3 cut(s) 648, 906, 1035
BspPI GGATC 3 cut(s) 1028, 1041, 1043
BspQI GCTCTTC 2 cut(s) 320, 328
BspT107I GGYRCC 1 cut(s) 904
BsrBI CCGCTC 3 cut(s) 217, 321, 538
BsrFI RCCGGY 1 cut(s) 242
BsrI ACTGG 4 cut(s) 476, 555, 595, 658
BssAI RCCGGY 1 cut(s) 242
BssECI CCNNGG 7 cut(s) 390, 391, 408, 486, 729, 873, 916
BssMI GATC 5 cut(s) 123, 807, 840, 1033, 1048
BssSI CACGAG 1 cut(s) 525
BssT1I CCWWGG 2 cut(s) 729, 916
Bst2BI CACGAG 1 cut(s) 525
Bst2UI CCWGG 1 cut(s) 874
Bst4CI ACNGT 2 cut(s) 386, 490
Bst6I CTCTTC 4 cut(s) 320, 328, 752, 1035
BstBAI YACGTR 1 cut(s) 951
BstC8I GCNNGC 4 cut(s) 215, 363, 540, 1000
BstDEI CTNAG 2 cut(s) 32, 510
BstDSI CCRYGG 2 cut(s) 486, 729
BstENI CCTNNNNNAGG 1 cut(s) 915
BstF5I GGATG 2 cut(s) 107, 225
BstHHI GCGC 1 cut(s) 572
BstKTI GATC 5 cut(s) 126, 810, 843, 1036, 1051
BstMBI GATC 5 cut(s) 123, 807, 840, 1033, 1048
BstMCI CGRYCG 1 cut(s) 126
BstMWI GCNNNNNNNGC 3 cut(s) 210, 347, 591
BstNI CCWGG 1 cut(s) 874
BstSCI CCNGG 5 cut(s) 88, 390, 391, 642, 872
BstSLI GKGCMC 1 cut(s) 391
BstV1I GCAGC 6 cut(s) 154, 328, 575, 578, 697, 1014
BstX2I RGATCY 1 cut(s) 1033
BstXI CCANNNNNNTGG 1 cut(s) 978
BstYI RGATCY 1 cut(s) 1033
BsuRI GGCC 1 cut(s) 106
BtgI CCRYGG 2 cut(s) 486, 729
BtgZI GCGATG 1 cut(s) 241
BtsCI GGATG 2 cut(s) 107, 225
BtsI GCAGTG 1 cut(s) 237
BtsIMutI CAGTG 6 cut(s) 237, 469, 548, 602, 651, 836
Cac8I GCNNGC 4 cut(s) 215, 363, 540, 1000
CciI TCATGA 1 cut(s) 132
CfoI GCGC 1 cut(s) 572
Cfr10I RCCGGY 1 cut(s) 242
Cfr13I GGNCC 1 cut(s) 646
Cfr9I CCCGGG 1 cut(s) 391
Csp6I GTAC 4 cut(s) 356, 424, 792, 905
CviAII CATG 6 cut(s) 133, 288, 304, 559, 631, 730
CviQI GTAC 4 cut(s) 356, 424, 792, 905
DdeI CTNAG 2 cut(s) 32, 510
DpnI GATC 5 cut(s) 125, 809, 842, 1035, 1050
DpnII GATC 5 cut(s) 123, 807, 840, 1033, 1048
DraI TTTAAA 1 cut(s) 196
DraIII CACNNNGTG 1 cut(s) 157
DriI GACNNNNNGTC 1 cut(s) 622
EaeI YGGCCR 1 cut(s) 104
Eam1104I CTCTTC 4 cut(s) 320, 328, 752, 1035
Eam1105I GACNNNNNGTC 1 cut(s) 622
EarI CTCTTC 4 cut(s) 320, 328, 752, 1035
Eco130I CCWWGG 2 cut(s) 729, 916
Eco47I GGWCC 1 cut(s) 646
Eco88I CYCGRG 2 cut(s) 391, 407
EcoNI CCTNNNNNAGG 1 cut(s) 915
EcoRI GAATTC 1 cut(s) 944
EcoRII CCWGG 1 cut(s) 872
EcoT14I CCWWGG 2 cut(s) 729, 916
EcoT22I ATGCAT 1 cut(s) 61
ErhI CCWWGG 2 cut(s) 729, 916
FaeI CATG 6 cut(s) 136, 291, 307, 562, 634, 733
FatI CATG 6 cut(s) 132, 287, 303, 558, 630, 729
Fnu4HI GCNGC 9 cut(s) 143, 235, 339, 342, 586, 589, 592, 711, 1003
FokI GGATG 2 cut(s) 114, 232
Fsp4HI GCNGC 9 cut(s) 143, 235, 339, 342, 586, 589, 592, 711, 1003
FspBI CTAG 1 cut(s) 1056
GlaI GCGC 1 cut(s) 571
GluI GCNGC 9 cut(s) 143, 235, 339, 342, 586, 589, 592, 711, 1003
HaeIII GGCC 1 cut(s) 106
HapII CCGG 6 cut(s) 89, 243, 392, 644, 1031, 1037
HhaI GCGC 1 cut(s) 572
Hin1II CATG 6 cut(s) 136, 291, 307, 562, 634, 733
Hin6I GCGC 1 cut(s) 570
HinP1I GCGC 1 cut(s) 570
HindIII AAGCTT 2 cut(s) 275, 464
HinfI GANTC 3 cut(s) 7, 440, 601
HpaII CCGG 6 cut(s) 89, 243, 392, 644, 1031, 1037
HphI GGTGA 2 cut(s) 430, 714
Hpy188I TCNGA 3 cut(s) 101, 670, 1053
Hpy188III TCNNGA 4 cut(s) 133, 452, 1031, 1037
HpyAV CCTTC 7 cut(s) 92, 139, 470, 651, 735, 950, 976
HpyCH4III ACNGT 2 cut(s) 386, 490
HpyCH4IV ACGT 2 cut(s) 369, 950
HpyCH4V TGCA 4 cut(s) 59, 287, 307, 558
HpyF10VI GCNNNNNNNGC 3 cut(s) 210, 347, 591
HpyF3I CTNAG 2 cut(s) 32, 510
HpySE526I ACGT 2 cut(s) 369, 950
Hsp92II CATG 6 cut(s) 136, 291, 307, 562, 634, 733
HspAI GCGC 1 cut(s) 570
Kpn2I TCCGGA 2 cut(s) 1030, 1036
KpnI GGTACC 1 cut(s) 908
Kzo9I GATC 5 cut(s) 123, 807, 840, 1033, 1048
LguI GCTCTTC 2 cut(s) 320, 328
Lsp1109I GCAGC 6 cut(s) 154, 328, 575, 578, 697, 1014
MaeI CTAG 1 cut(s) 1056
MaeII ACGT 2 cut(s) 369, 950
MaeIII GTNAC 5 cut(s) 245, 520, 733, 779, 959
MalI GATC 5 cut(s) 125, 809, 842, 1035, 1050
MbiI CCGCTC 3 cut(s) 217, 321, 538
MboI GATC 5 cut(s) 123, 807, 840, 1033, 1048
MboII GAAGA 7 cut(s) 60, 307, 315, 537, 739, 860, 1052
MfeI CAATTG 1 cut(s) 800
MflI RGATCY 1 cut(s) 1033
MhlI GDGCHC 2 cut(s) 40, 391
MlsI TGGCCA 1 cut(s) 106
MluCI AATT 5 cut(s) 11, 689, 800, 944, 966
MluNI TGGCCA 1 cut(s) 106
MlyI GAGTC 2 cut(s) 434, 610
MnlI CCTC 8 cut(s) 262, 403, 487, 755, 826, 891, 921, 925
Mox20I TGGCCA 1 cut(s) 106
Mph1103I ATGCAT 1 cut(s) 61
MroI TCCGGA 2 cut(s) 1030, 1036
MroXI GAANNNNTTC 2 cut(s) 314, 1026
MscI TGGCCA 1 cut(s) 106
MseI TTAA 2 cut(s) 195, 639
MslI CAYNNNNRTG 1 cut(s) 563
Msp20I TGGCCA 1 cut(s) 106
MspA1I CMGCKG 1 cut(s) 341
MspI CCGG 6 cut(s) 89, 243, 392, 644, 1031, 1037
MspR9I CCNGG 5 cut(s) 90, 392, 393, 644, 874
MunI CAATTG 1 cut(s) 800
Mva1269I GAATGC 1 cut(s) 59
MvaI CCWGG 1 cut(s) 874
MwoI GCNNNNNNNGC 3 cut(s) 210, 347, 591
NciI CCSGG 4 cut(s) 90, 392, 393, 644
NcoI CCATGG 1 cut(s) 729
NdeII GATC 5 cut(s) 123, 807, 840, 1033, 1048
NlaIII CATG 6 cut(s) 136, 291, 307, 562, 634, 733
NlaIV GGNNCC 3 cut(s) 648, 906, 1035
NmuCI GTSAC 3 cut(s) 245, 520, 779
NsiI ATGCAT 1 cut(s) 61
PagI TCATGA 1 cut(s) 132
PciSI GCTCTTC 2 cut(s) 320, 328
PcsI WCGNNNNNNNCGW 1 cut(s) 264
PctI GAATGC 1 cut(s) 59
PdmI GAANNNNTTC 2 cut(s) 314, 1026
PflMI CCANNNNNTGG 1 cut(s) 113
PkrI GCNGC 9 cut(s) 144, 236, 340, 343, 587, 590, 593, 712, 1004
Ple19I CGATCG 1 cut(s) 126
PleI GAGTC 2 cut(s) 434, 609
PpsI GAGTC 2 cut(s) 434, 609
Ppu21I YACGTR 1 cut(s) 951
Psp6I CCWGG 1 cut(s) 872
PspGI CCWGG 1 cut(s) 872
PspN4I GGNNCC 3 cut(s) 648, 906, 1035
PspPI GGNCC 1 cut(s) 646
PsuI RGATCY 1 cut(s) 1033
PvuI CGATCG 1 cut(s) 126
RsaI GTAC 4 cut(s) 357, 425, 793, 906
RsaNI GTAC 4 cut(s) 356, 424, 792, 905
RseI CAYNNNNRTG 1 cut(s) 563
SapI GCTCTTC 2 cut(s) 320, 328
SaqAI TTAA 2 cut(s) 195, 639
SatI GCNGC 9 cut(s) 143, 235, 339, 342, 586, 589, 592, 711, 1003
Sau3AI GATC 5 cut(s) 123, 807, 840, 1033, 1048
Sau96I GGNCC 1 cut(s) 646
SchI GAGTC 2 cut(s) 434, 610
ScrFI CCNGG 5 cut(s) 90, 392, 393, 644, 874
SduI GDGCHC 2 cut(s) 40, 391
SinI GGWCC 1 cut(s) 646
SmaI CCCGGG 1 cut(s) 393
SmiMI CAYNNNNRTG 1 cut(s) 563
Sse9I AATT 5 cut(s) 11, 689, 800, 944, 966
SsiI CCGC 7 cut(s) 217, 234, 319, 339, 359, 536, 585
SspI AATATT 1 cut(s) 869
SspMI CTAG 1 cut(s) 1056
StyD4I CCNGG 5 cut(s) 88, 390, 391, 642, 872
StyI CCWWGG 2 cut(s) 729, 916
TaaI ACNGT 2 cut(s) 386, 490
TaiI ACGT 2 cut(s) 372, 953
TaqI TCGA 1 cut(s) 701
TasI AATT 5 cut(s) 11, 689, 800, 944, 966
TatI WGTACW 1 cut(s) 423
TauI GCSGC 3 cut(s) 237, 341, 588
Tru1I TTAA 2 cut(s) 195, 639
Tru9I TTAA 2 cut(s) 195, 639
TscAI CASTG 6 cut(s) 244, 476, 555, 602, 658, 843
TseFI GTSAC 3 cut(s) 245, 520, 779
TseI GCWGC 6 cut(s) 142, 341, 588, 591, 710, 1002
Tsp45I GTSAC 3 cut(s) 245, 520, 779
TspDTI ATGAA 8 cut(s) 121, 149, 185, 244, 619, 690, 878, 969
TspGWI ACGGA 1 cut(s) 589
TspMI CCCGGG 1 cut(s) 391
TspRI CASTG 6 cut(s) 244, 476, 555, 602, 658, 843
Van91I CCANNNNNTGG 1 cut(s) 113
VpaK11BI GGWCC 1 cut(s) 646
XagI CCTNNNNNAGG 1 cut(s) 915
XapI RAATTY 2 cut(s) 689, 944
XmaI CCCGGG 1 cut(s) 391
XmnI GAANNNNTTC 2 cut(s) 314, 1026
XspI CTAG 1 cut(s) 1056
Zsp2I ATGCAT 1 cut(s) 61
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.