Rroxscaffold_5G00339220

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0000778
Physical Location & Seq
Reverse (-)
7578799 .. 7581554
2756 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00339220.1

Sequence Viewer

Length: 1455 bp
ATGGATTACAGTAACCTTTACATTTTCTTGTCTACACTACTTGCCATCTTCACTACTACTACTCTTGCATTCCTTTTTTCTGCTTACAAGTCAAAATCCCATGAAAAATCCATTAGTCTTCCCCCAGGCAGTTTTGGGTGGCCGATTATTGGCGAAACCTTTGCCTTCCTACACGATGACCATGAAAAATTTGTAGGGGATAGGATGAAGAAATACTCCTCCAAAATCTTCAAAACCAAGATACTAGGTGAACGAACCGTGGTGTTATGTGGCACTGCCGGTCACAAATTTGTGGCTTCCAACGAAGAAAAGCTCTTTGCAGCATGGAGACCTCACTCAATGCAAAAGCTCTTTCGTTCTTCATACCAAAAAGCTGCTTCCACCATCATGCCAAGACAAATGGAAAAGCATGTATCTCAGGCACCGGGGTTTCTGAGAGCCGAAGCCCTGGTTGGCTACGTGGCAGCGATGGATTCCATGGTTCTCGACCACCTGAAAACGCACTGGGATGGCAAACAAGTGGTCGAGGCTTATCGCCTCACGCAGCTACTCGTTTTGACTCTCTCAGCCAAGTTCTTCATGGGGCTCGAAGACAGCTGTCGTATTGGTAAGCTTGTCGAGTTGATGGACACCATGATGATGGCGCTTCACACTATTCCTGTGAATATTCCGGGGACTGCATTTCATCGAGCAATGAAAGCAGCGCCTGCTGCGAGGGAGATTATTCAGTCGTTTGTTAAGGAGAAGAAAGAAGTTATGGAGTCTACCGGAAACAAAATGCATGATCTTTTGTCTTATATGATTGCTACGCCAGACCCAACCGGAAGGTTCATGCCGGAGAATGAAATTGCTGATAAAATTATGGGGAATGTGGCCGCTTCTTTCAACTCTCCAGCTATGACTACTACTTTTATCGTGAAGTTCCTGGGCGAAAGACCTGACATATATGACAAAGTCCGAATTGAACAACTGGAGATTTTGAAGTCCAAAAAATCTGGCGAGGCACTCAATTGGGATGATATGCAAAAAATGAAGTATTCGTGGAACGTAGCCCTGGAGGCGATGAGGCTCATACCACCTCTTCAGGGAACATTTAGAGAGGCTGCAACAGACTTCACTTACCAAGGTTATACAATTCCTAAAGGATGGAAGGTCTACTGGACAGTGAGCACCACAACCATGAACCCTGAATATTTTCCAGCGCCAGAAGAGTTTGACCCTACAAGGTTCGAGAAAGCAACTCCGCCTTACACAAACATCCCATTCGGGAGTGGTCCTCGAATTTGCCCCGGAAAGGACTACGCTCGTCTGCAAATTCTTAGTTTTATGCACCATCTTGTTATAAGATTCAAATGGGAACTTGTGAACCCTAATTGCAAGATCACAGGTGGTATGAATCCCTTTCCAGTTGATGGGCTGATGGTTCGTCTCCAAGCTCAACCCAGTCAAGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
p450

Protein Analysis

484

Amino Acids

54.87

Weight (kDa)

9.09

Isoelectric Point (pI)

34.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 41 - 456 3e-55 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1343
AccB1I GGYRCC 1 cut(s) 421
AccB7I CCANNNNNTGG 1 cut(s) 1412
AccI GTMKAC 3 cut(s) 32, 764, 1155
AciI CCGC 2 cut(s) 876, 1244
AcoI YGGCCR 2 cut(s) 140, 873
AcsI RAATTY 4 cut(s) 188, 287, 1281, 1314
AcuI CTGAAG 1 cut(s) 1067
AfiI CCNNNNNNNGG 4 cut(s) 149, 1085, 1294, 1412
AgsI TTSAA 5 cut(s) 232, 886, 965, 982, 1351
AjnI CCWGG 4 cut(s) 124, 447, 924, 1053
AjuI GAANNNNNNNTTGG 2 cut(s) 435, 467
AluBI AGCT 9 cut(s) 313, 349, 374, 547, 597, 613, 896, 1436, 1451
AluI AGCT 9 cut(s) 313, 349, 374, 547, 597, 613, 896, 1436, 1451
Alw21I GWGCWC 1 cut(s) 1172
Alw26I GTCTC 2 cut(s) 322, 1433
AlwNI CAGNNNCTG 1 cut(s) 707
AoxI GGCC 2 cut(s) 140, 873
ApeKI GCWGC 7 cut(s) 320, 374, 464, 544, 701, 710, 1103
ApoI RAATTY 4 cut(s) 188, 287, 1281, 1314
ArsI GACNNNNNNTTYG 2 cut(s) 507, 539
Asp700I GAANNNNTTC 1 cut(s) 1194
AspLEI GCGC 3 cut(s) 646, 706, 1204
AspS9I GGNCC 1 cut(s) 1274
AsuC2I CCSGG 3 cut(s) 426, 672, 1290
AsuHPI GGTGA 1 cut(s) 260
AvaII GGWCC 1 cut(s) 1274
BanI GGYRCC 1 cut(s) 421
BanII GRGCYC 1 cut(s) 588
BbsI GAAGAC 2 cut(s) 110, 597
Bbv12I GWGCWC 1 cut(s) 1172
BbvI GCAGC 7 cut(s) 332, 361, 476, 556, 697, 713, 1090
BcgI CGANNNNNNTGC 2 cut(s) 143, 177
BciT130I CCWGG 4 cut(s) 126, 449, 926, 1055
BcnI CCSGG 3 cut(s) 426, 672, 1290
BcoDI GTCTC 2 cut(s) 322, 1433
BfaI CTAG 1 cut(s) 245
BfoI RGCGCY 3 cut(s) 647, 707, 1205
BglI GCCNNNNNGGC 1 cut(s) 1058
BisI GCNGC 8 cut(s) 321, 375, 465, 545, 702, 711, 876, 1104
BlsI GCNGC 8 cut(s) 322, 376, 466, 546, 703, 712, 877, 1105
Bme1390I CCNGG 7 cut(s) 126, 426, 449, 672, 926, 1055, 1290
Bme18I GGWCC 1 cut(s) 1274
BmgT120I GGNCC 1 cut(s) 1274
BmiI GGNNCC 1 cut(s) 423
BmrFI CCNGG 7 cut(s) 126, 426, 449, 672, 926, 1055, 1290
BmrI ACTGGG 2 cut(s) 514, 1437
BmuI ACTGGG 2 cut(s) 514, 1437
BoxI GACNNNNGTC 1 cut(s) 597
BpiI GAAGAC 2 cut(s) 110, 597
BplI GAGNNNNNCTC 2 cut(s) 1261, 1293
BpmI CTGGAG 3 cut(s) 876, 992, 1076
BpuMI CCSGG 3 cut(s) 426, 672, 1290
BsaAI YACGTR 1 cut(s) 460
BsaI GGTCTC 1 cut(s) 322
BsaWI WCCGGW 2 cut(s) 767, 821
Bsc4I CCNNNNNNNGG 4 cut(s) 149, 1085, 1294, 1412
Bse118I RCCGGY 1 cut(s) 278
Bse1I ACTGG 5 cut(s) 509, 975, 1163, 1406, 1443
Bse3DI GCAATG 1 cut(s) 699
BseBI CCWGG 4 cut(s) 126, 449, 926, 1055
BseGI GGATG 5 cut(s) 210, 514, 1021, 1151, 1257
BseLI CCNNNNNNNGG 4 cut(s) 149, 1085, 1294, 1412
BseMI GCAATG 1 cut(s) 699
BseMII CTCAG 3 cut(s) 425, 431, 579
BseNI ACTGG 5 cut(s) 509, 975, 1163, 1406, 1443
BseRI GAGGAG 1 cut(s) 208
BseXI GCAGC 7 cut(s) 332, 361, 476, 556, 697, 713, 1090
BshFI GGCC 2 cut(s) 142, 875
BshNI GGYRCC 1 cut(s) 421
BsiHKAI GWGCWC 1 cut(s) 1172
BsiSI CCGG 7 cut(s) 279, 425, 671, 768, 822, 836, 1290
BslFI GGGAC 1 cut(s) 688
BslI CCNNNNNNNGG 4 cut(s) 149, 1085, 1294, 1412
BsmAI GTCTC 2 cut(s) 322, 1433
BsmBI CGTCTC 1 cut(s) 1433
BsmFI GGGAC 1 cut(s) 688
BsmI GAATGC 1 cut(s) 68
BsnI GGCC 2 cut(s) 142, 875
Bso31I GGTCTC 1 cut(s) 322
Bsp1286I GDGCHC 2 cut(s) 588, 1172
Bsp143I GATC 2 cut(s) 784, 1380
Bsp19I CCATGG 1 cut(s) 477
BspACI CCGC 2 cut(s) 876, 1244
BspANI GGCC 2 cut(s) 142, 875
BspCNI CTCAG 3 cut(s) 426, 430, 578
BspLI GGNNCC 1 cut(s) 423
BspT107I GGYRCC 1 cut(s) 421
BspTNI GGTCTC 1 cut(s) 322
BsrDI GCAATG 1 cut(s) 699
BsrFI RCCGGY 1 cut(s) 278
BsrI ACTGG 5 cut(s) 509, 975, 1163, 1406, 1443
BssAI RCCGGY 1 cut(s) 278
BssMI GATC 2 cut(s) 784, 1380
BssT1I CCWWGG 2 cut(s) 477, 1123
Bst2UI CCWGG 4 cut(s) 126, 449, 926, 1055
Bst4CI ACNGT 3 cut(s) 11, 259, 1165
Bst6I CTCTTC 2 cut(s) 1086, 1203
BstAPI GCANNNNNTGC 1 cut(s) 707
BstBAI YACGTR 1 cut(s) 460
BstC8I GCNNGC 1 cut(s) 708
BstDEI CTNAG 4 cut(s) 417, 434, 565, 1319
BstDSI CCRYGG 2 cut(s) 258, 477
BstF5I GGATG 5 cut(s) 210, 514, 1021, 1151, 1257
BstH2I RGCGCY 3 cut(s) 647, 707, 1205
BstHHI GCGC 3 cut(s) 646, 706, 1204
BstKTI GATC 2 cut(s) 787, 1383
BstMAI GTCTC 2 cut(s) 322, 1433
BstMBI GATC 2 cut(s) 784, 1380
BstMWI GCNNNNNNNGC 4 cut(s) 698, 707, 710, 1058
BstNI CCWGG 4 cut(s) 126, 449, 926, 1055
BstNSI RCATGY 1 cut(s) 413
BstPAI GACNNNNGTC 1 cut(s) 597
BstSCI CCNGG 7 cut(s) 124, 424, 447, 670, 924, 1053, 1288
BstV1I GCAGC 7 cut(s) 332, 361, 476, 556, 697, 713, 1090
BstV2I GAAGAC 2 cut(s) 110, 597
BstXI CCANNNNNNTGG 1 cut(s) 640
BsuRI GGCC 2 cut(s) 142, 875
BtgI CCRYGG 2 cut(s) 258, 477
BtgZI GCGATG 2 cut(s) 482, 1076
BtsCI GGATG 5 cut(s) 210, 514, 1021, 1151, 1257
BtsI GCAGTG 1 cut(s) 273
BtsIMutI CAGTG 3 cut(s) 273, 502, 1170
Cac8I GCNNGC 1 cut(s) 708
CaiI CAGNNNCTG 1 cut(s) 707
CfoI GCGC 3 cut(s) 646, 706, 1204
Cfr10I RCCGGY 1 cut(s) 278
Cfr13I GGNCC 1 cut(s) 1274
DdeI CTNAG 4 cut(s) 417, 434, 565, 1319
DpnI GATC 2 cut(s) 786, 1382
DpnII GATC 2 cut(s) 784, 1380
EaeI YGGCCR 2 cut(s) 140, 873
Eam1104I CTCTTC 2 cut(s) 1086, 1203
EarI CTCTTC 2 cut(s) 1086, 1203
EciI GGCGGA 1 cut(s) 1233
Eco130I CCWWGG 2 cut(s) 477, 1123
Eco24I GRGCYC 1 cut(s) 588
Eco31I GGTCTC 1 cut(s) 322
Eco47I GGWCC 1 cut(s) 1274
Eco57I CTGAAG 1 cut(s) 1067
EcoRII CCWGG 4 cut(s) 124, 447, 924, 1053
EcoT14I CCWWGG 2 cut(s) 477, 1123
EcoT22I ATGCAT 1 cut(s) 783
EcoT38I GRGCYC 1 cut(s) 588
ErhI CCWWGG 2 cut(s) 477, 1123
Esp3I CGTCTC 1 cut(s) 1433
FaqI GGGAC 1 cut(s) 688
FblI GTMKAC 3 cut(s) 32, 764, 1155
Fnu4HI GCNGC 8 cut(s) 321, 375, 465, 545, 702, 711, 876, 1104
FokI GGATG 5 cut(s) 217, 521, 1028, 1158, 1244
FriOI GRGCYC 1 cut(s) 588
Fsp4HI GCNGC 8 cut(s) 321, 375, 465, 545, 702, 711, 876, 1104
FspBI CTAG 1 cut(s) 245
GlaI GCGC 3 cut(s) 645, 705, 1203
GluI GCNGC 8 cut(s) 321, 375, 465, 545, 702, 711, 876, 1104
GsuI CTGGAG 3 cut(s) 876, 992, 1076
HaeII RGCGCY 3 cut(s) 647, 707, 1205
HaeIII GGCC 2 cut(s) 142, 875
HapII CCGG 7 cut(s) 279, 425, 671, 768, 822, 836, 1290
HhaI GCGC 3 cut(s) 646, 706, 1204
Hin6I GCGC 3 cut(s) 644, 704, 1202
HinP1I GCGC 3 cut(s) 644, 704, 1202
HindIII AAGCTT 2 cut(s) 611, 1449
HinfI GANTC 5 cut(s) 473, 559, 761, 1347, 1396
HpaII CCGG 7 cut(s) 279, 425, 671, 768, 822, 836, 1290
HphI GGTGA 1 cut(s) 260
Hpy166II GTNNAC 5 cut(s) 33, 251, 765, 1156, 1366
Hpy188I TCNGA 2 cut(s) 435, 959
Hpy188III TCNNGA 4 cut(s) 485, 916, 1231, 1267
Hpy8I GTNNAC 5 cut(s) 33, 251, 765, 1156, 1366
HpyAV CCTTC 3 cut(s) 175, 819, 1144
HpyCH4III ACNGT 3 cut(s) 11, 259, 1165
HpyCH4IV ACGT 2 cut(s) 459, 1047
HpyF10VI GCNNNNNNNGC 4 cut(s) 698, 707, 710, 1058
HpyF3I CTNAG 4 cut(s) 417, 434, 565, 1319
HpySE526I ACGT 2 cut(s) 459, 1047
HspAI GCGC 3 cut(s) 644, 704, 1202
Kzo9I GATC 2 cut(s) 784, 1380
Lsp1109I GCAGC 7 cut(s) 332, 361, 476, 556, 697, 713, 1090
MaeI CTAG 1 cut(s) 245
MaeII ACGT 2 cut(s) 459, 1047
MaeIII GTNAC 2 cut(s) 11, 281
MalI GATC 2 cut(s) 786, 1382
MboI GATC 2 cut(s) 784, 1380
MfeI CAATTG 1 cut(s) 1009
MhlI GDGCHC 2 cut(s) 588, 1172
MlyI GAGTC 2 cut(s) 553, 770
MmeI TCCRAC 1 cut(s) 324
Mph1103I ATGCAT 1 cut(s) 783
MroXI GAANNNNTTC 1 cut(s) 1194
MseI TTAA 2 cut(s) 738, 1453
MslI CAYNNNNRTG 5 cut(s) 386, 507, 635, 638, 1178
MspA1I CMGCKG 1 cut(s) 597
MspI CCGG 7 cut(s) 279, 425, 671, 768, 822, 836, 1290
MspR9I CCNGG 7 cut(s) 126, 426, 449, 672, 926, 1055, 1290
MunI CAATTG 1 cut(s) 1009
Mva1269I GAATGC 1 cut(s) 68
MvaI CCWGG 4 cut(s) 126, 449, 926, 1055
MwoI GCNNNNNNNGC 4 cut(s) 698, 707, 710, 1058
NciI CCSGG 3 cut(s) 426, 672, 1290
NcoI CCATGG 1 cut(s) 477
NdeII GATC 2 cut(s) 784, 1380
NlaIV GGNNCC 1 cut(s) 423
NmuCI GTSAC 1 cut(s) 281
NsiI ATGCAT 1 cut(s) 783
NspI RCATGY 1 cut(s) 413
PctI GAATGC 1 cut(s) 68
PdmI GAANNNNTTC 1 cut(s) 1194
PfeI GAWTC 3 cut(s) 473, 1347, 1396
PflFI GACNNNGTC 1 cut(s) 953
PflMI CCANNNNNTGG 1 cut(s) 1412
PkrI GCNGC 8 cut(s) 322, 376, 466, 546, 703, 712, 877, 1105
PleI GAGTC 2 cut(s) 553, 769
PpsI GAGTC 2 cut(s) 553, 769
Ppu21I YACGTR 1 cut(s) 460
PshAI GACNNNNGTC 1 cut(s) 597
PsiI TTATAA 1 cut(s) 1343
Psp6I CCWGG 4 cut(s) 124, 447, 924, 1053
PspGI CCWGG 4 cut(s) 124, 447, 924, 1053
PspN4I GGNNCC 1 cut(s) 423
PspPI GGNCC 1 cut(s) 1274
PstNI CAGNNNCTG 1 cut(s) 707
PsyI GACNNNGTC 1 cut(s) 953
PvuII CAGCTG 1 cut(s) 597
RseI CAYNNNNRTG 5 cut(s) 386, 507, 635, 638, 1178
SaqAI TTAA 2 cut(s) 738, 1453
SatI GCNGC 8 cut(s) 321, 375, 465, 545, 702, 711, 876, 1104
Sau3AI GATC 2 cut(s) 784, 1380
Sau96I GGNCC 1 cut(s) 1274
SchI GAGTC 2 cut(s) 553, 770
ScrFI CCNGG 7 cut(s) 126, 426, 449, 672, 926, 1055, 1290
SduI GDGCHC 2 cut(s) 588, 1172
SinI GGWCC 1 cut(s) 1274
SmiMI CAYNNNNRTG 5 cut(s) 386, 507, 635, 638, 1178
SsiI CCGC 2 cut(s) 876, 1244
SspI AATATT 2 cut(s) 667, 1193
SspMI CTAG 1 cut(s) 245
StyD4I CCNGG 7 cut(s) 124, 424, 447, 670, 924, 1053, 1288
StyI CCWWGG 2 cut(s) 477, 1123
TaaI ACNGT 3 cut(s) 11, 259, 1165
TaiI ACGT 2 cut(s) 462, 1050
TaqI TCGA 7 cut(s) 486, 525, 588, 618, 688, 1230, 1279
TauI GCSGC 1 cut(s) 878
TfiI GAWTC 3 cut(s) 473, 1347, 1396
Tru1I TTAA 2 cut(s) 738, 1453
Tru9I TTAA 2 cut(s) 738, 1453
TscAI CASTG 3 cut(s) 280, 509, 1170
TseFI GTSAC 1 cut(s) 281
TseI GCWGC 7 cut(s) 320, 374, 464, 544, 701, 710, 1103
Tsp45I GTSAC 1 cut(s) 281
TspRI CASTG 3 cut(s) 280, 509, 1170
Tth111I GACNNNGTC 1 cut(s) 953
Van91I CCANNNNNTGG 1 cut(s) 1412
VpaK11BI GGWCC 1 cut(s) 1274
XapI RAATTY 4 cut(s) 188, 287, 1281, 1314
XceI RCATGY 1 cut(s) 413
XcmI CCANNNNNNNNNTGG 1 cut(s) 577
XmiI GTMKAC 3 cut(s) 32, 764, 1155
XmnI GAANNNNTTC 1 cut(s) 1194
XspI CTAG 1 cut(s) 245
Zsp2I ATGCAT 1 cut(s) 783
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.